The curved leucine-rich repeat region of Toll-like receptors, represented here by TLR3
Toll-like receptors (TLRs) are a class of proteins that play a key role in the innate immune system. They are single, membrane-spanning, non-catalytic receptors usually expressed in sentinel cells such as macrophages and dendritic cells, that recognize structurally conserved molecules derived from microbes. Once these microbes have breached physical barriers such as the skin or intestinal tract mucosa, they are recognized by TLRs, which activate immune cell responses. The TLRs include TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10, TLR11, TLR12, and TLR13, though the latter three are not found in human.
They received their name from their similarity to the protein coded by the toll gene identified in Drosophila in 1985 by Christiane Nüsslein-Volhard.[1] The researchers were so surprised that they spontaneously shouted out in German, "Das ist ja toll!" which translates as "That's great!"[2]
Contents
- 1 Diversity
- 2 Discovery
- 3 Extended family
- 3.1 TLRs in Drosophila immunity
- 3.2 TLR2
- 3.3 TLR3
- 3.4 TLR11
- 4 Ligands
- 5 Signaling
- 5.1 MyD88-dependent pathway
- 5.2 TRIF-dependent pathway
- 6 Summary of known mammalian TLRs
- 7 Activation and effects
- 8 Drugs interactions
- 9 Notes and references
- 10 See also
- 11 External links
Diversity
TLRs are a type of pattern recognition receptor (PRR) and recognize molecules that are broadly shared by pathogens but distinguishable from host molecules, collectively referred to as pathogen-associated molecular patterns (PAMPs). TLRs together with the Interleukin-1 receptors form a receptor superfamily, known as the "interleukin-1 receptor / toll-like receptor superfamily"; all members of this family have in common a so-called TIR (toll-IL-1 receptor) domain.
Three subgroups of TIR domains exist. Proteins with subgroup 1 TIR domains are receptors for interleukins that are produced by macrophages, monocytes, and dendritic cells and all have extracellular Immunoglobulin (Ig) domains. Proteins with subgroup 2 TIR domains are classical TLRs, and bind directly or indirectly to molecules of microbial origin. A third subgroup of proteins containing TIR domains consists of adaptor proteins that are exclusively cytosolic and mediate signaling from proteins of subgroups 1 and 2.
TLRs are present in vertebrates, as well as in invertebrates. Molecular building blocks of the TLRs are represented in bacteria and in plants, and plant pattern recognition receptors are well known to be required for host defence against infection. The TLRs thus appear to be one of the most ancient, conserved components of the immune system.
In recent years TLRs were identified also in the mammalian nervous system. Members of the TLR family were detected on glia, neurons and on neural progenitor cells in which they regulate cell-fate decision.[3]
Discovery
When microbes were first recognized as the cause of infectious diseases, it was immediately clear that multicellular organisms must be capable of recognizing them when infected and, hence, capable of recognizing molecules unique to microbes. A large body of literature, spanning most of the last century, attests to the search for the key molecules and their receptors. More than 100 years ago, Richard Pfeiffer, a student of Robert Koch, coined the term "endotoxin" to describe a substance produced by Gram-negative bacteria that could provoke fever and shock in experimental animals. In the decades that followed, endotoxin was chemically characterized and identified as a lipopolysaccharide (LPS) produced by most Gram-negative bacteria. This lipopolysaccharide is an integral part of the gram-negative membrane and is released upon destruction of the bacterium. Other molecules (bacterial lipopeptides, flagellin, and unmethylated DNA) were shown in turn to provoke host responses that are normally protective. However, these responses can be detrimental if they are excessively prolonged or intense. It followed logically that there must be receptors for such molecules, capable of alerting the host to the presence of infection, but these remained elusive for many years.
Toll-like receptors are now counted among the key molecules that alert the immune system to the presence of microbial infections. They are named for their similarity to toll, a receptor first identified in the fruit fly Drosophila melanogaster, and originally known for its developmental function in that organism. In 1996, toll was found by Jules A. Hoffmann and his colleagues to have an essential role in the fly's immunity to fungal infection,[4] which it achieved by activating the synthesis of antimicrobial peptides. The plant homologs were discovered by Pamela Ronald in 1995 (rice XA21)[5] and Thomas Boller in 2000 (Arabidopsis FLS2).[6]
The first reported human toll-like receptor was described by Nomura and colleagues in 1994,[7] mapped to a chromosome by Taguchi and colleagues in 1996.[8] Because the immune function of toll in Drosophila was not then known, it was assumed that TIL (now known as TLR1) might participate in mammalian development. However, in 1991 (prior to the discovery of TIL) it was observed that a molecule with a clear role in immune function in mammals, the interleukin-1 (IL-1) receptor, also had homology to drosophila toll; the cytoplasmic portions of both molecules were similar.[9]
In 1997, Charles Janeway and Ruslan Medzhitov showed that a toll-like receptor now known as TLR4 could, when artificially ligated using antibodies, induce the activation of certain genes necessary for initiating an adaptive immune response.[10] TLR 4 function as an LPS sensing receptor was discovered by Bruce A. Beutler and colleagues.[11] These workers used positional cloning to prove that mice that could not respond to LPS had mutations that abolished the function of TLR4. This identified TLR4 as one of the key components of the receptor for LPS.
In turn, the other TLR genes were ablated in mice by gene targeting, largely in the laboratory of Shizuo Akira and colleagues. Each TLR is now believed to detect a discrete collection of molecules – some of microbial origin, and some products of cell damage – and to signal the presence of infections.
On October 3, 2011, Dr. Beutler and Dr. Hoffmann were awarded the Nobel Prize in Medicine or Physiology for their work.[12] Drs. Hoffmann and Akira received the Canada Gairdner International Award in 2011.[13]
Extended family
It has been estimated that most mammalian species have between ten and fifteen types of toll-like receptors. Thirteen TLRs (named simply TLR1 to TLR13) have been identified in humans and mice together, and equivalent forms of many of these have been found in other mammalian species.[14][15][16] However, equivalents of certain TLR found in humans are not present in all mammals. For example, a gene coding for a protein analogous to TLR10 in humans is present in mice, but appears to have been damaged at some point in the past by a retrovirus. On the other hand, mice express TLRs 11, 12, and 13, none of which is represented in humans. Other mammals may express TLRs that are not found in humans. Other non-mammalian species may have TLRs distinct from mammals, as demonstrated by TLR14, which is found in the Takifugu pufferfish.[17] This may complicate the process of using experimental animals as models of human innate immunity.
TLRs in Drosophila immunity
Drosophila melanogaster has only innate immune responses. Response to fungal or bacterial infection occurs through two distinct signalling cascades, one of which is toll pathway and the other is immune deficiency (IMD) pathway. The toll pathway is similar to mammalian TLR signalling, but unlike mammalian TLRs, toll is not activated directly by PAMPs. Its receptor ectodomain recognizes cleaved form of the cytokine Spätzle, which is secreted in the haemolymph as inactive dimeric precursor. Toll receptor shares the cytoplasmatic TIR domain with mammalian TLRs, but ectodomain and intracytoplasmatic tail are different. This difference might reflect a function of these receptors as cytokine receptors rather than PRRs. Toll pathway is activated by different stimuli, such as Gram positive bacteria, fungi and virulence factors. First, the Spätzle processing enzyme (SPE) is activated in response to infection and cleaves Spätzle. Cleaved Spätzle then binds to toll receptor and crosslinks its ectodomains. This triggers conformational changes in receptor resulting in signalling through toll. Signalling now is very similar to mammalian signalling through TLRs. Toll-induced signalling complex (TICS) is formed, comprising MyD88, Tube and Pelle( the orthologue of mammalian IRAK). Signal from TICS is then transduced to Cactus (homologue of mammalian IκB), phosphorylated Cactus is polyubiquitylated and degraded, allowing nuclear translocation of DIF (dorsal-related immunity facor; a homologue of mammalian NF-κB) and induction of transcription of genes for antimicrobial peptides (AMPs) such as Drosomycin.[18]
TLR2
Main article: TLR2
TLR2 has also been designated as CD282 (cluster of differentiation 282).
TLR3
TLR3 is the only toll-like receptor which does not use the MyD88 dependent pathway. Its ligand is retroviral double-stranded RNA (dsRNA), which activates the TRIF dependent signalling pathway. To explore the role of this pathway in retroviral reprograming, knock down techniques of TLR3 or TRIF were prepared, and results showed that only the TLR3 pathway is required for full induction of target gene expression by the retrovirus expression vector. This retroviral expression of four transcriptional factors (Oct4, Sox2, Klf4 and c-Myc; OSKM) induces pluripotency in somatic cells. This is supported by study, which shows, that efficiency and amount of human iPSC generation, using retroviral vectors, is reduced by knockdown of the pathway with peptide inhibitors or shRNA knockdown of TLR3 or its adaptor protein TRIF. Taken together, stimulation of TLR3 causes great changes in chromatin remodeling and nuclear reprogramming, and activation of inflammatory pathways is required for these changes, induction of pluripotency genes and generation of human induced pluripotent stem cells (iPSC) colonies.[19]
TLR11
As mentioned above, human cells do not express TLR11, but mice do. It has been characterized, that mouse-specific TLR11 recognizes uropathogenic E.coli and apicomplexan parasite Toxoplasma gondii. Its ligand is profilin protein in case of Toxoplasma, but ligand for E. coli is still not known. Recently there was found another enteropathogen which ligand is bound by TLR11. It is Salmonella spp. This gram-negative flagellated bacteria causes food- and waterborne gastroenteritis and typhoid fever in humans. TLR11 in mouse intestine recognizes this flagellin which causes dimerization of the receptor, activation of NF-κB and production of inflammatory cytokines. TLR11 deficient mice (Knockout mouse) are efficiently infected with orally administered Salmonella Typhi. S. Typhi does not normally infect mice, it is human obligatory pathogen that causes typhoid fever, which affects more than 20 million people and causes more than 220 thousand deaths per year. Because of this, studies were carried out and it was found that tlr-/- mice can be immunized against S. Typhi and they are used as an animal model for studying immune responses against this pathogen and for the development of vaccines, that could be possibly used in the future.[20]
Ligands
Because of the specificity of toll-like receptors (and other innate immune receptors) they cannot easily be changed in the course of evolution, these receptors recognize molecules that are constantly associated with threats (i.e., pathogen or cell stress) and are highly specific to these threats (i.e., cannot be mistaken for self molecules that are normally expressed under physiological conditions). Pathogen-associated molecules that meet this requirement are thought to be critical to the pathogen's function and difficult to change through mutation; they are said to be evolutionarily conserved. Somewhat conserved features in pathogens include bacterial cell-surface lipopolysaccharides (LPS), lipoproteins, lipopeptides, and lipoarabinomannan; proteins such as flagellin from bacterial flagella; double-stranded RNA of viruses; or the unmethylated CpG islands of bacterial and viral DNA; and also of the CpG islands found in the promoters of eukaryotic DNA; as well as certain other RNA and DNA molecules. For most of the TLRs, ligand recognition specificity has now been established by gene targeting (also known as "gene knockout"): a technique by which individual genes may be selectively deleted in mice.[21][22] See the table below for a summary of known TLR ligands.
Endogenous ligands
The stereotypic inflammatory response provoked by toll Like-Receptor activation has prompted speculation that endogenous activators of toll-like receptors might participate in autoimmune diseases. TLRs have been suspected of binding to host molecules including fibrinogen (involved in blood clotting), heat shock proteins (HSPs), HMGB1, extracellular matrix components and self DNA (it is normally degraded by nucleases, but under inflammatory and autoimmune conditions it can form a complex with endogenous proteins, become resistant to these nucleases and gain access to endosomal TLRs as TLR7 or TLR9). These endogenous ligands are usually produced as a result of non-physiological cell death.[23]
Different TLRs can recognize different antigens as listed below.
TLR-1:- Bacterial lipoprotein and peptidoglycans
TLR-2:- Bacterial peptidoglycans
TLR-3:- Double stranded RNA
TLR-4:- Lipopolysaccharides
TLR-5:- Bacterial flagella
TLR-6:- Bacterial lipoprotein
TLR-7:- Single stranded RNA
TLR-8:- Single stranded RNA
TLR-9:- CpG DNA
TLR-10:- Unknown
Signaling
Signaling pathway of toll-like receptors. Dashed grey lines represent unknown associations.
TLRs are believed to function as dimers. Though most TLRs appear to function as homodimers, TLR2 forms heterodimers with TLR1 or TLR6, each dimer having a different ligand specificity. TLRs may also depend on other co-receptors for full ligand sensitivity, such as in the case of TLR4's recognition of LPS, which requires MD-2. CD14 and LPS-Binding Protein (LBP) are known to facilitate the presentation of LPS to MD-2.
A set of endosomal TLRs comprising TLR3, TLR7, TLR8 and TLR9 recognize nucleic acid derived from viruses as well as endogenous nucleic acids in context of pathogenic events. Activation of these receptor leads to production of inflammatory cytokines as well as type I interferons (interferon type I) to help fighting viral infection.
The adapter proteins and kinases that mediate TLR signaling have also been targeted. In addition, random germline mutagenesis with ENU has been used to decipher the TLR signaling pathways. When activated, TLRs recruit adapter molecules within the cytoplasm of cells in order to propagate a signal. Four adapter molecules are known to be involved in signaling. These proteins are known as MyD88, Tirap (also called Mal), Trif, and Tram (toll-like receptor 4 adaptor protein).[24][25][26]
TLR signaling is divided into two distinct signaling pathways, the MyD88-dependent and TRIF-dependent pathway.
MyD88-dependent pathway
The MyD88-dependent response occurs on dimerization of the TLR receptor, and is utilized by every TLR except TLR3. Its primary effect is activation of NFκB and Mitogen-activated protein kinase. Ligand binding and conformational change that occurs in the receptor recruits the adaptor protein MyD88, a member of the TIR family. MyD88 then recruits IRAK4, IRAK1 and IRAK2. IRAK kinases then phosphorylate and activate the protein TRAF6, which in turn polyubiquinates the protein TAK1, as well as itself in order to facilitate binding to IKK-β. On binding, TAK1 phosphorylates IKK-β, which then phosphorylates IκB causing its degradation and allowing NFκB to diffuse into the cell nucleus and activate transcription and consequent induction of inflammatory cytokines.[23]
TRIF-dependent pathway
Both TLR3 and TLR4 utilize the TRIF-dependent pathway, which is triggered by dsRNA and LPS, respectively. For TLR3, dsRNA leads to activation of the receptor, recruiting the adaptor TRIF. TRIF activates the kinases TBK1 and RIPK1, which creates a branch in the signaling pathway. The TRIF/TBK1 signaling complex phosphorylates IRF3 allowing its translocation into the nucleus and production of Interferon type I. Meanwhile, activation of RIPK1 causes the polyubiquitination and activation of TAK1 and NFκB transcription in the same manner as the MyD88-dependent pathway.[23]
TLR signaling ultimately leads to the induction or suppression of genes that orchestrate the inflammatory response. In all, thousands of genes are activated by TLR signaling, and collectively, the TLRs constitute one of the most pleiotropic yet tightly regulated gateways for gene modulation.
TLR4 is the only TLR that uses all four adaptors. Complex consisting of TLR4, MD2 and LPS recruits TIR domain-containing adaptors TIRAP and MyD88 and thus initiates activation of NFκB (early phase) and MAPK. TLR4-MD2-LPS complex then undergoes endocytosis and in endosome it forms a signalling complex with TRAM and TRIF adaptors. This TRIF-dependent pathway again leads to IRF3 activation and production of type I interferons, but it also activates late-phase NFκB activation. Both late and early phase activation of NFκB is required for production of inflammatory cytokines.[23]
Summary of known mammalian TLRs
Toll-like receptors bind and become activated by different ligands, which, in turn, are located on different types of organisms or structures. They also have different adapters to respond to activation and are located sometimes at the cell surface and sometimes to internal cell compartments. Furthermore, they are expressed by different types of leucocytes or other cell types:
| Receptor |
Ligand(s)[27] |
Ligand location[27] |
Adapter(s) |
Location |
Cell types[27] |
| TLR 1 |
multiple triacyl lipopeptides |
Bacterial lipoprotein |
MyD88/MAL |
cell surface |
- monocytes/macrophages
- a subset of dendritic cells
- B lymphocytes
|
| TLR 2 |
multiple glycolipids |
Bacterial peptidoglycans |
MyD88/MAL |
cell surface |
- monocytes/macrophages
- neutrophils [28]
- Myeloid dendritic cells[29]
- Mast cells
|
| multiple lipopeptides |
Bacterial peptidoglycans |
| multiple lipoproteins |
Bacterial peptidoglycans |
| lipoteichoic acid |
Gram-positive bacteria |
| HSP70 |
Host cells |
| zymosan (Beta-glucan) |
Fungi |
| Numerous others |
|
| TLR 3 |
double-stranded RNA, poly I:C |
viruses |
TRIF |
cell compartment |
- Dendritic cells
- B lymphocytes
|
| TLR 4 |
lipopolysaccharide |
Gram-negative bacteria |
MyD88/MAL/TRIF/TRAM |
cell surface |
- monocytes/macrophages
- neutrophils [28]
- Myeloid dendritic cells[29]
- Mast cells
- B lymphocytes [30]
- Intestinal epithelium
|
| several heat shock proteins |
Bacteria and host cells |
| fibrinogen |
host cells |
| heparan sulfate fragments |
host cells |
| hyaluronic acid fragments |
host cells |
| nickel |
|
| Various opioid drugs |
|
| TLR 5 |
Bacterial flagellin |
Bacteria |
MyD88 |
cell surface |
- monocyte/macrophages
- a subset of dendritic cells
- Intestinal epithelium
|
| [[profilin[31]]] |
Toxoplasma gondii |
| TLR 6 |
multiple diacyl lipopeptides |
Mycoplasma |
MyD88/MAL |
cell surface |
- monocytes/macrophages
- Mast cells
- B lymphocytes
|
| TLR 7 |
imidazoquinoline |
small synthetic compounds |
MyD88 |
cell compartment |
- monocytes/macrophages
- Plasmacytoid dendritic cells[29]
- B lymphocytes
|
| loxoribine (a guanosine analogue) |
| bropirimine |
| single-stranded RNA |
RNA viruses |
| TLR 8 |
small synthetic compounds; single-stranded RNA |
|
MyD88 |
cell compartment |
- monocytes/macrophages
- a subset of dendritic cells
- Mast cells
|
| TLR 9 |
unmethylated CpG Oligodeoxynucleotide DNA |
Bacteria, DNA viruses |
MyD88 |
cell compartment |
- monocytes/macrophages
- Plasmacytoid dendritic cells[29]
- B lymphocytes
|
| TLR 10 |
unknown |
|
unknown |
? |
|
| TLR 11 |
Profilin |
Toxoplasma gondii[32] |
MyD88 |
cell compartment[33] |
- monocytes/macrophages
- liver cells
- kidney
- urinary bladder epithelium
|
| TLR 12 |
Profilin |
Toxoplasma gondii[34] |
MyD88 |
|
- Neurons[35]
- plasmacytoid dendritic cells
- conventional dendritic cells
- macrophages
|
| TLR 13 [36][37] |
bacterial ribosomal RNA sequence "CGGAAAGACC" |
Virus, bacteria |
MyD88, TAK-1 |
cell compartment |
- monocytes/macrophages
- conventional dendritic cells
|
Activation and effects
Following activation by ligands of microbial origin, several reactions are possible. Immune cells can produce signalling factors called cytokines, which trigger inflammation. In the case of a bacterial factor, the pathogen might be phagocytosed and digested, and its antigens presented to CD4+ T cells. In the case of a viral factor, the infected cell may shut off its protein synthesis and may undergo programmed cell death (apoptosis). Immune cells that have detected a virus may also release anti-viral factors such as interferons.
The discovery of the toll-like receptors finally identified the innate immune receptors that are responsible for many of the innate immune functions that had been studied for many years. It is interesting to note that TLRs seem to be involved only in the cytokine production and cellular activation in response to microbes, and do not play a significant role in the adhesion and phagocytosis of microorganisms.
Schmidt et al. demonstrated that TLR4 is involved in the development of contact allergy to nickel in humans.[38] By binding to two non-conserved histidines, H456 and H458, Ni2+ cross-links the two receptor monomers, TLR4, and MD2, triggering formation of a dimer that structurally resembles the one induced by Lipopolysaccharide. That, in turn, activates the proinflammatory intracellular signal transduction cascades.
Said et al. showed that TLR ligands cause an IL-10-dependent inhibition of CD4 T-cell expansion and function by up-regulating PD-1 levels on monocytes, which leads to IL-10 production by monocytes after binding of PD-1 by PD-L.[39]
Toll-like receptors have also been shown to be an important link between innate and adaptive immunity through their presence in dendritic cells. The TLRs 3 and 4 are present on the surface of monocyte derived dendritic cells and use the Myd88-dependent pathway to produce interleukins 12 and 18 which signal naive T-cells to mature into type 1 helper T cells. These TLRs also use the TRIF pathway to upregulate costimulatory proteins which aid in the differentiation of T-cells. TLRs 7 and 9 are present on the endosome of plasmacytoid dendritic cells. These proteins solely make use of the Myd88 dependent pathway to produce interleukins for the maturation of naive T-cells to type 1 helper T-cells. Toll-like receptors have also been shown to be expressed on immune cells like T cells.[40] Flagellin, a TLR-5 ligand induces cytokine secretion on interacting with TLR-5 on human T cells.[40]
Evelyn A. Kurt-Jones et al. also demonstrated TLR4s role in the innate immune response to the respiratory syncytial virus. Cytokine production increased upon exposing human monocytes to RSV. After knocking out CD14, TLR4's coactivator, this response was significantly diminished, as were the responses in C3H/HeJ mice, a mouse strain with reduced immune responses, and mice deficient in TLR4.
Drugs interactions
Imiquimod (cardinally used in dermatology), and its successor resiquimod, are ligands for TLR7 and TLR8.[41]
The lipid A analogon eritoran acts as a TLR4 antagonist. As of December 2009[update], it is being developed as a drug against severe sepsis.[42]
Notes and references
- ^ Hansson GK, Edfeldt K (2005). "Toll to be paid at the gateway to the vessel wall". Arterioscler. Thromb. Vasc. Biol. 25 (6): 1085–7. doi:10.1161/01.ATV.0000168894.43759.47. PMID 15923538.
- ^ http://www.aerzteblatt.de/archiv/55316/
- ^ Rolls A., Shechter R., London A. et al. (September 2007). "Toll-like receptors modulate adult hippocampal neurogenesis". Nat. Cell Biol. 9 (9): 1081–8. doi:10.1038/ncb1629. PMID 17704767.
- ^ Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA (September 1996). "The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults". Cell 86 (6): 973–83. doi:10.1016/S0092-8674(00)80172-5. PMID 8808632.
- ^ Song, W.Y.; Wang, G.-L.; Chen, L.-L.; Kim, H.-S.; Pi, L.-Y.; Holsten, T.; Gardner, J.; Wang, B.; Zhai, W.-X.; Zhu, L.-H.; Fauquet, C.; Ronald, P. et al. (1995). "A receptor kinase-like protein encoded by the rice disease resistance gene, XA21". Science 270 (5243): 1804–1806. Bibcode:1995Sci...270.1804S. doi:10.1126/science.270.5243.1804. PMID 8525370.
- ^ Gomez-Gomez, L.; Boller, Thomas et al. (2000). "FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis". Molecular Cell 5 (6): 1003–1011. doi:10.1016/S1097-2765(00)80265-8. PMID 10911994.
- ^ Nomura N, Miyajima N, Sazuka T et al. (1994). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1" (– Scholar search). DNA Res. 1 (1): 27–35. doi:10.1093/dnares/1.1.27. PMID 7584026. [dead link]
- ^ Taguchi T, Mitcham JL, Dower SK, Sims JE, Testa JR (March 1996). "Chromosomal localization of TIL, a gene encoding a protein related to the Drosophila transmembrane receptor Toll, to human chromosome 4p14". Genomics 32 (3): 486–8. doi:10.1006/geno.1996.0150. PMID 8838819.
- ^ Gay NJ, Keith FJ; Keith (May 1991). "Drosophila Toll and IL-1 receptor". Nature 351 (6325): 355–6. Bibcode:1991Natur.351..355G. doi:10.1038/351355b0. PMID 1851964.
- ^ Medzhitov R, Preston-Hurlburt P, Janeway CA (July 1997). "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity". Nature 388 (6640): 394–7. doi:10.1038/41131. PMID 9237759.
- ^ Poltorak A, He X, Smirnova I; He; Smirnova; Liu; Van Huffel; Du; Birdwell; Alejos; Silva; Galanos; Freudenberg; Ricciardi-Castagnoli; Layton; Beutler et al. (December 1998). "Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene". Science 282 (5396): 2085–8. Bibcode:1998Sci...282.2085P. doi:10.1126/science.282.5396.2085. PMID 9851930.
- ^ http://www.nobelprize.org/nobel_prizes/medicine/laureates/2011/press.html
- ^ Mitchell, Bob (23 March 2011). "B.C. doctor wins prestigious medical prize". The Star.
- ^ Du X, Poltorak A, Wei Y, Beutler B (September 2000). "Three novel mammalian toll-like receptors: gene structure, expression, and evolution". Eur. Cytokine Netw. 11 (3): 362–71. PMID 11022119.
- ^ Chuang TH, Ulevitch RJ (September 2000). "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9". Eur. Cytokine Netw. 11 (3): 372–8. PMID 11022120.
- ^ Tabeta K, Georgel P, Janssen E; Georgel; Janssen; Du; Hoebe; Crozat; Mudd; Shamel; Sovath; Goode; Alexopoulou; Flavell; Beutler et al. (March 2004). "Toll-like receptors 9 and 3 as essential components of innate immune defense against mouse cytomegalovirus infection". Proc. Natl. Acad. Sci. U.S.A. 101 (10): 3516–21. Bibcode:2004PNAS..101.3516T. doi:10.1073/pnas.0400525101. PMC 373494. PMID 14993594.
- ^ Roach JC, Glusman G, Rowen L, Kaur A, Purcell MK, Smith KD, Hood LE, Aderem A; Glusman; Rowen; Kaur; Purcell; Smith; Hood; Aderem (2005). "The evolution of vertebrate Toll-like receptors" (PDF). Proc Natl Acad Sci USA 102 (27): 9577–9582. Bibcode:2005PNAS..102.9577R. doi:10.1073/pnas.0502272102. PMC 1172252. PMID 15976025.
- ^ Ferrandon D, Imler JL, Hetru C, Hoffmann JA (2007) The Drosophila systemic immune response: sensing and signalling during bacterial and fungal infections. Nat Rev Immunol 7 (11):862-74. doi:10.1038/nri2194 PMID 17948019
- ^ Lee J, Sayed N, Hunter A, Au KF, Wong WH, Mocarski ES et al. (2012) Activation of innate immunity is required for efficient nuclear reprogramming. Cell 151 (3):547-58. doi:10.1016/j.cell.2012.09.034 PMID 23101625
- ^ Mathur R, Oh H, Zhang D, Park SG, Seo J, Koblansky A et al. (2012) A mouse model of Salmonella typhi infection. Cell 151 (3):590-602. doi:10.1016/j.cell.2012.08.042 PMID 23101627
- ^ Hoebe K, Du X, Georgel P; Du; Georgel; Janssen; Tabeta; Kim; Goode; Lin; Mann; Mudd; Crozat; Sovath; Han; Beutler et al. (August 2003). "Identification of Lps2 as a key transducer of MyD88-independent TIR signalling". Nature 424 (6950): 743–8. Bibcode:2003Natur.424..743H. doi:10.1038/nature01889. PMID 12872135.
- ^ Hemmi H, Takeuchi O, Kawai T et al. (December 2000). "A Toll-like receptor recognizes bacterial DNA". Nature 408 (6813): 740–5. doi:10.1038/35047123. PMID 11130078.
- ^ a b c d Kawai, Taro; Shizuo Akira (20 April 2010). "The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors". Nature Immunology 11 (5). doi:10.1038/ni.1863.
- ^ Shigeoka AA, Holscher TD, King AJ et al. (May 2007). "TLR2 is constitutively expressed within the kidney and participates in ischemic renal injury through both MyD88-dependent and -independent pathways". J. Immunol. 178 (10): 6252–8. doi:10.4049/jimmunol.178.10.6252. PMID 17475853.
- ^ Yamamoto M, Sato S, Hemmi H et al. (November 2003). "TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway". Nat. Immunol. 4 (11): 1144–50. doi:10.1038/ni986. PMID 14556004.
- ^ Yamamoto M, Sato S, Hemmi H; Sato; Hemmi; Sanjo; Uematsu; Kaisho; Hoshino; Takeuchi; Kobayashi; Fujita; Takeda; Akira et al. (November 2002). "Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4". Nature 420 (6913): 324–9. Bibcode:2002Natur.420..324Y. doi:10.1038/nature01182. PMID 12447441.
- ^ a b c Unless else specified in boxes then ref is: Waltenbaugh C, Doan T, Melvold R, Viselli S (2008). Immunology. Lippincott's Illustrated reviews. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. p. 17. ISBN 0-7817-9543-5.
- ^ a b Sabroe, Ian; Dower SK; Whyte MKB (2005). "The Role of Toll-Like Receptors in the Regulation of Neutrophil Migration, Activation, and Apoptosis". Clinical Infectious Diseases. 41 Suppl 7: S421–426. doi:10.1086/431992. PMID 16237641.
- ^ a b c d Sallusto F, Lanzavecchia A (2002). "The instructive role of dendritic cells on T-cell responses". Arthritis Res. 4 Suppl 3: S127–32. doi:10.1186/ar567. PMC 3240143. PMID 12110131.
- ^ Gerondakis, Steve; Grumont RJ; Banerjee A (2007). "Regulating B-cell activation and survival in response to TLR signals". Immunology and Cell Biology 85 (6): 471–475. doi:10.1038/sj.icb.7100097. PMID 17637697. Retrieved 21 August 2011.
- ^ http://www.ncbi.nlm.nih.gov/pubmed/?term=Toxoplasma+gondii-Derived+Profilin+Triggers+Human+Toll-Like+Receptor+5-Dependent+Cytokine+Production
- ^ Yarovinsky F, Zhang D, Andersen JF, Bannenberg GL, Serhan CN, Hayden MS, Hieny S, Sutterwala FS, Flavell RA, Ghosh S, Sher A. (2005). "TLR11 activation of dendritic cells by a protozoan profilin-like protein". Science 308 (5728): 1626–9. doi:10.1126/science.1109893. PMID 15860593.
- ^ Pifer R, Benson A, Sturge CR and Yarovinsky F (November 2010). "UNC93B1 is essential for TLR11 activation and IL-12 dependent host resistance to Toxoplasma Gondii". Journal of Biological Chemistry.doi:10.1074/jbc.M110.171025 PMID 21097503
- ^ Koblansky AA, Jankovic D, Oh H, Hieny S, Sungnak W, Mathur R, Hayden MS, Akira S, Sher A, Ghosh S. (2012). "Recognition of Profilin by Toll-like Receptor 12 Is Critical for Host Resistance to Toxoplasma gondii". Immunity 38 (1): 119–30. doi:10.1016/j.immuni.2012.09.016. PMID 23246311.
- ^ Mishra BB, Gundra UM, Teale JM (2008). "Expression and distribution of Toll-like receptors 11-13 in the brain during murine neurocysticercosis". Journal of Neuroinflammation 5: 53. doi:10.1186/1742-2094-5-53. PMC 2631477. PMID 19077284.
- ^ Shi Z, Cai Z, Sanchez A et al. (February 2011). "A novel Toll-like receptor that recognizes vesicular stomatitis virus". Journal of Biological Chemistry 286 (6): 4517–24. doi:10.1074/jbc.M110.159590. PMC 3039399. PMID 21131352.
- ^ Oldenburg M, Kruger A, Ferstl R; Krüger; Ferstl; Kaufmann; Nees; Sigmund; Bathke; Lauterbach; Suter; Dreher; Koedel; Akira; Kawai; Buer; Wagner; Bauer; Hochrein; Kirschning et al. (August 2012). "TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification". Science 337 (6098): 1111–5. Bibcode:2012Sci...337.1111O. doi:10.1126/science.1220363. PMID 22821982.
- ^ M. Schmidt, B. Raghavan, V. Müller, T. Vogl, G. Fejer, S. Tchaptchet, S. Keck, C. Kalis, P. J. Nielsen, C. Galanos, J. Roth, A. Skerra, S. F. Martin, M. A. Freudenberg, M. Goebeler. Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel. Nature Immunology 11: 814-819 (2010).
- ^ Elias A. Said et al. 2009, PD-1 Induced IL10 Production by Monocytes Impairs T-cell Activation in a Reversible Fashion" Nature Medicine 2010; 452-9.
- ^ a b Sharma, N et al. (2013). "Sphingosine-1-phosphate suppresses TLR-induced CXCL8 secretion from human T cells.". J Leukoc Biol 93 (4): 521–528. doi:10.1189/jlb.0712328. PMID 23345392.
- ^ Peter Fritsch (2004). Dermatologie Venerologie : Grundlagen. Klinik. Atlas. (in German). Berlin: Springer. ISBN 3-540-00332-0.
- ^ Tidswell, M; Tillis, W; Larosa, SP; Lynn, M; Wittek, AE; Kao, R; Wheeler, J; Gogate, J et al. (2010). "Phase 2 trial of eritoran tetrasodium (E5564), a Toll-like receptor 4 antagonist, in patients with severe sepsis". Critical Care Medicine 38 (1): 72–83. doi:10.1097/CCM.0b013e3181b07b78. PMID 19661804.
See also
- NOD-like receptor
- Immunologic adjuvant
External links
- Toll-Like Receptors at the US National Library of Medicine Medical Subject Headings (MeSH)
- TollML: Toll-like receptors and ligands database at University of Munich
- The Toll-Like Receptor Family of Innate Immune Receptors (pdf)
- Toll-Like receptor Pathway
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Transmembrane receptors: immunoglobulin superfamily immune receptors
|
|
Antibody receptor:
Fc receptor |
| Epsilon (ε) |
- FcεRI
- (FcεRII is C-type lectin)
|
|
| Gamma (γ) |
- FcγRI
- FcγRII
- FcγRIII
- Neonatal
|
|
| Alpha (α)/mu (μ) |
|
|
| Secretory |
- Polymeric immunoglobulin receptor
|
|
|
| Antigen receptor |
| B cells |
| Antigen receptor |
|
|
| Co-receptor |
|
|
| Accessory molecules |
|
|
|
| T cells |
| Ligands |
|
|
| Antigen receptor |
|
|
| Co-receptors |
- CD8 (with two glycoprotein chains CD8α and CD8β)
- CD4
|
|
| Accessory molecules |
- CD3
- CD3γ
- CD3δ
- CD3ε
- ζ-chain (also called CD3ζ and TCRζ)
|
|
|
|
| Cytokine receptor |
|
|
| Killer-cell IG-like receptors |
- KIR2DL1
- KIR2DL2
- KIR2DL3
- KIR2DL4
- KIR2DL5A
- KIR2DL5B
- KIR2DS1
- KIR2DS2
- KIR2DS3
- KIR2DS4
- KIR2DS5
- KIR3DL1
- KIR3DL2
- KIR3DL3
- KIR3DS1
|
|
| Leukocyte IG-like receptors |
- LILRA1
- LILRA2
- LILRA3
- LILRA4
- LILRA5
- LILRA6
- LILRB1
- LILRB2
- LILRB3
- LILRB4
- LILRB5
- LILRA6
- LILRA5
|
|
|
Index of signal transduction
|
|
| Description |
- Intercellular
- neuropeptides
- growth factors
- cytokines
- hormones
- Cell surface receptors
- ligand-gated
- enzyme-linked
- G protein-coupled
- immunoglobulin superfamily
- integrins
- neuropeptide
- growth factor
- cytokine
- Intracellular
- adaptor proteins
- GTP-binding
- MAP kinase
- Calcium signaling
- Lipid signaling
- Pathways
- hedgehog
- Wnt
- TGF beta
- MAPK ERK
- notch
- JAK-STAT
- apoptosis
- hippo
- TLR
|
|
Index of the immune system
|
|
| Description |
- Physiology
- cells
- autoantigens
- autoantibodies
- complement
- surface antigens
- IG receptors
|
|
| Disease |
- Allergies
- Immunodeficiency
- Immunoproliferative immunoglobulin disorders
- Hypersensitivity and autoimmune disorders
- Neoplasms and cancer
|
|
| Treatment |
- Procedures
- Drugs
- antihistamines
- immunostimulants
- immunosuppressants
- monoclonal antibodies
|
|
|
|
Signaling pathway: TLR signaling pathway
|
|
| Receptor |
|
|
| Other external |
|
|
| Internal |
- adaptor: MYD88
- TRIF
- TIRAP
- TRAF6
- TOLLIP
|
|
|
|
|
|
|
|
|
|
|
|
|
Index of signal transduction
|
|
| Description |
- Intercellular
- neuropeptides
- growth factors
- cytokines
- hormones
- Cell surface receptors
- ligand-gated
- enzyme-linked
- G protein-coupled
- immunoglobulin superfamily
- integrins
- neuropeptide
- growth factor
- cytokine
- Intracellular
- adaptor proteins
- GTP-binding
- MAP kinase
- Calcium signaling
- Lipid signaling
- Pathways
- hedgehog
- Wnt
- TGF beta
- MAPK ERK
- notch
- JAK-STAT
- apoptosis
- hippo
- TLR
|
|
|
