- 関
- PCR、PCR amplification、polymerase chain reaction
WordNet
- a quantitative or qualitative test of a substance (especially an ore or a drug) to determine its components; frequently used to test for the presence or concentration of infectious agents or antibodies etc.
- analyze (chemical substances)
- a substance that is undergoing an analysis of its components
- an appraisal of the state of affairs; "they made an assay of the contents"; "a check on its dependability under stress" (同)check
- a written report of the results of an analysis of the composition of some substance
- hardy and sure-footed animal smaller and with longer ears than the horse
- a pompous fool
- the 16th letter of the Roman alphabet (同)p
PrepTutorEJDIC
- (金属の品質・鉱石の金属含有量・薬品の)分析,試金 / 試金物,分析物 / …'を'試金する,分析する
- ロバ / ばか者
- 《米俗》しり,けつ(《英俗》arse)
- Peace Corps 平和部隊
- parking
- phosphorusの化学記号
UpToDate Contents
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English Journal
- [The testing strategy for detection of biologically relevant infection of human papillomavirus in head and neck tumors for routine pathological analysis].
- Kašpírková J, Ondič O, Cerná K, Skálová A.AbstractThere is a subgroup among head and neck squamous cell carcinomas, which is etiologically linked to the infection of high-risk human papillomavirus (HPV). In recent studies, HPV related squamous cell carcinomas have been placed in a separate group because of their different epidemiology, distinctive histopathological characteristics, therapeutic response and clinical outcome. The reported prevalence of high-risk HPV in head and neck tumors varies in different studies. This fact occurs mainly due to the absence of a widely accepted consensus for HPV detection in head and neck malignancies. We present a methodological algorithm for detection of biologically relevant HPV infection: a combination of an immunohistochemical staining of the p16 protein - a surrogate marker for a transforming HPV infection, and a molecular genetic identification of HPV DNA by three different polymerase chain reactions (PCR). The study group consisted of 41 patients with a tumor in head and neck region. A verification of detection of biologically relevant HPV infection has been performed in 10 available samples using an alternative approach, which comprised the detection of RNA transcript of HPV by reverse transcription followed by PCR (RT-PCR), and further in situ hybridization (ISH) with a commercial high-risk HPV probe. We have found a high correlation between HPV DNA detection using triple-PCR approach and strong diffuse positivity of the p16 protein (correlation coefficient 0.94) and have confirmed the validity of this algorithm. In 94 % of HPV related squamous cell carcinomas HPV type 16 was detected. In one case HPV type 33 was identified. That is in agreement with earlier published data. A more appropriate alternative method for the detection of biologically relevant- transforming HPV infection seems to be RT-PCR, which proved 100 % agreement with the original methodological approach of p16 determination and PCR status. Interpretation of the ISH has been complicated by frequent nonspecific staining of the sample and its routine usage in the diagnostic algorithm of our laboratory is currently not feasible.Keywords: HPV - squamous cell carcinoma - head and neck tumors - p16.
- Ceskoslovenská patologie.Cesk Patol.2013 Dec;49(1):29-34.
- There is a subgroup among head and neck squamous cell carcinomas, which is etiologically linked to the infection of high-risk human papillomavirus (HPV). In recent studies, HPV related squamous cell carcinomas have been placed in a separate group because of their different epidemiology, distinctive
- PMID 23432073
- RNA blood levels of osteopontin splice variants are cancer markers.
- Hartung F, Weber GF.SourceUniversity of Cincinnati Academic Health Center, College of Pharmacy, 3225 Eden Avenue, Cincinnati, OH 45267-0004 USA.
- SpringerPlus.Springerplus.2013 Dec;2(1):110. Epub 2013 Mar 14.
- PURPOSE: Despite a sizeable and continuously growing literature on osteopontin and cancer the molecule has not yet found entry into clinical diagnostics. Our identification of spliced variants that are more specific for cancer than the full-length transcript has opened new possibilities for reaching
- PMID 23543917
- Cold tolerance in Osmotin transgenic tomato (Solanum lycopersicum L.) is associated with modulation in transcript abundance of stress responsive genes.
- Patade VY, Khatri D, Kumari M, Grover A, Mohan Gupta S, Ahmed Z.SourceMolecular Biology and Genetic Engineering Division, Defence Institute of Bio-Energy Research, Haldwani, 263 139 Uttarakhand INDIA.
- SpringerPlus.Springerplus.2013 Dec;2(1):117. Epub 2013 Mar 19.
- Our containment trials have established cold tolerance in Nicotiana tabacum osmotin (Nt Osm) transgenic tomato (Solanum lycopersicum L. cv. Pusa Ruby). Though, the stress tolerance mechanisms have been studied at physio-biochemical levels, molecular mechanisms underlying the tolerant response are st
- PMID 23543825
Japanese Journal
- PCR-Invaderを用いたHCV NS5A領域Y93H変異検出法の開発
- Takano Shingo,Ishikawa Eiichi,Matsuda Masahide,Yamamoto Tetsuya,Matsumura Akira
- International journal of oncology 45(5), 1837-1846, 2014-11
- … The effect of IFN-β for these cell lines were evaluated by means of proliferation (MTT assay), conditioned medium induced HUVEC migration, VEGF and interferon inducible protein 10 (IP10, angiogenesis inhibitor) expression by RT-PCR and western blot analysis. …
- NAID 120005496691
- 2012/2013シーズンにおけるインフルエンザウイルスA型流行株に認められたneuraminidase遺伝子変異とインフルエンザウイルスPCR検査法の改良
- 栁田 光利,桑村 良隆,木下 悟 [他]
- 臨床病理 : 日本臨床検査医学会誌 62(10), 937-941, 2014-10
- NAID 40020267257
★リンクテーブル★
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- 英
- PCR、PCR assay、PCR amplification、polymerase chain reaction
- 関
- 酵素連鎖反応法、ポリメラーゼ連鎖反応法、遺伝子増幅法、核酸増幅法、PCR増幅、核酸増幅検査、ポリメラーゼ連鎖反応、polymerase chain reaction法
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- 関
- PCR、PCR assay、polymerase chain reaction
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- 英
- PCR assay
- 関
- PCR法
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- 関
- anal、analyse、analyses、analysis、analytical、analyze、determination、determine、dissect、method、method for measurement、-metry、quantitative determination、statistical method、test
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ポリメラーゼ連鎖反応 polymerase chain reaction