WordNet

put an end to; "The terrible news ended our hopes that he had survived"
a final state; "he came to a bad end"; "the so-called glorious experiment came to an inglorious end" (同)destruction, death
the point in time at which something ends; "the end of the year"; "the ending of warranty period" (同)ending
bring to an end or halt; "She ended their friendship when she found out that he had once been convicted of a crime"; "The attack on Poland terminated the relatively peaceful period after WW I" (同)terminate
a piece of cloth that is left over after the rest has been used or sold (同)remainder, remnant, oddment
(American football) a position on the line of scrimmage; "no one wanted to play end"
the part you are expected to play; "he held up his end"
the concluding parts of an event or occurrence; "the end was exciting"; "I had to miss the last of the movie" (同)last, final_stage
either extremity of something that has length; "the end of the pier"; "she knotted the end of the thread"; "they rode to the end of the line"; "the terminals of the anterior arches of the fornix" (同)terminal
(football) the person who plays at one end of the line of scrimmage; "the end managed to hold onto the pass"
a boundary marking the extremities of something; "the end of town"
a final part or section; "we have given it at the end of the section since it involves the calculus"; "Start at the beginning and go on until you come to the end"
one of two places from which people are communicating to each other; "the phone rang at the other end"; "both ends wrote at the same time"
the surface at either extremity of a three-dimensional object; "one end of the box was marked `This side up"
have an end, in a temporal, spatial, or quantitative sense; either spatial or metaphorical; "the bronchioles terminate in a capillary bed"; "Your rights stop where you infringe upon the rights of other"; "My property ends by the bushes"; "The symphony ends in a pianissimo" (同)stop, finish, terminate, cease
be the end of; be the last or concluding part of; "This sad scene ended the movie" (同)terminate
event whose occurrence ends something; "his death marked the ending of an era"; "when these final episodes are broadcast it will be the finish of the show" (同)conclusion, finish
the end of a word (a suffix or inflectional ending or final morpheme); "I dont like words that have -ism as an ending" (同)termination
the 8th letter of the Roman alphabet (同)h
half the width of an em (同)nut

PrepTutorEJDIC

(細いものの)『端』,先端《+『of』+『名』》 / (物語などの)『終り』,終結部《+『of』+『名』》 / (物事・期間の)『最後』《+『of』+『名』》;(…に)結末をつけるもの《+『to』+『名』》 / (…の)端の部分,末端部《『of』+『名』》 / 《しばしば複数形で》『目的』(purpose),目標(aim) / 《遠回しに》死,滅亡 / 《しばしば複数形で》切れ端,くず,残りもの / (事業などの)部門(part) / (フットボールで)エンド)前衛両端の選手または位置) / …‘を'『終わらせる』,終える / 〈物事が〉…‘の'終りとなる,‘を'締めくくる / 『終わる』,終了する(come to an end)
終り,終了;(物語・映画などの)結末 / (文の)語尾
hydrogenの化学記号
鉛筆の硬度 / 《俗》heroin

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/12/29 20:11:03」(JST)

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[Wiki en表示]

The enzyme Endoglycosidase H (Endo-β-N-acetylglucosaminidase H, (EC 3.2.1.96) is a highly specific endoglycosidase which cleaves asparagine-linked mannose rich oligosaccharides, but not highly processed complex oligosaccharides from glycoproteins. It is used for research purposes to deglycosylate glycoproteins.

Structure and Activity

Endoglycosidase H is isolated from Streptomyces plicatus or Streptomyces griseus. Its molecular weight is 29 000 Daltons. The primary structure is described by Robbins 1984^[1]

Endoglycosidase H cleaves the bond in the diacetylchitobiose core of the oligosaccharide between two N-acetylglucosamine (GlcNAc) subunits directly proximal to the asparagine residue, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine.^[2]

It deglycosylates mannose glycoproteins, but the extent and rate of the deglycosylation depends to a high degree on the nature of the glycoproteins. The deglycosylation rate can be increased by denaturation of the glycoproteins (e.g., by carboxymethylation, sulfitolysis or by heating in the presence of sodium dodecyl sulfate). Over 0.02% SDS may inactivate the enzyme. The addition of 0.1 M 2-mercaptoethanol highly increases enzyme activity against glycoproteins containing inter- or intra-molecular disulfide bridges, unlike detergents like Triton X-100, n- Octylglucoside, or zwitterionic detergents.^[3]

Biochemical Applications

Endoglycosidase H (Endo H) is commonly used by cell biologists to monitor posttranslational modification in the Golgi apparatus. Most proteins destined for the cell surface are translated by ribosomes in the rough endoplasmic reticulum (ER) and translocated into the Golgi. Upon entering the ER a molecule containing 14 sugar subunits is linked en bloc to an asparagine in a selective manner by the enzyme oligosaccharyl transferase. It is this oligosaccharide molecule which is modified by a series of enzymes as the protein moves through the different compartments of the Golgi apparatus. Endoglycosidase H is able to cleave each structure of this oligosaccharide as it is processed until the enzyme Golgi alpha-mannosidase II removes two mannose subunits. Since all later oligosaccharide structures are resistant to Endo H cleavage the enzyme is widely used to report the extent of oligosaccharide processing a protein of interest has undergone.^[4]

References

^ Robbins P. W., R. B. Trimble, D. F. Wirth, C. Hering, F.Maley , G. F. Maley, R. Das, B. W. Gibson, N. Royal and K. Biemann. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H. J Biol Chem 259:7577-7583 (1984).
^ Endoglycosidase H, North Star
^ Trimble, R. B. & Maley, F. (1984) Anal. Biochem. 141, 515–522
^ Alberts et al. (2002) Molecular Biology of the Cell, Fourth Edition ISBN 0-8153-4072-9

External links

IUBMB Enzyme Nomenclature - EC 3.2.1.96

Close enzymes

Endoglycosidases F and D, cleave Glc-Nac

PNGase F (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine_amidase)

This enzyme-related article is a stub. You can help Wikipedia by expanding it.

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English Journal

Deterioration of pre-existing hemiparesis due to an ipsilateral internal capsule infarction after a contralateral stroke.

Kobayashi Z1, Akaza M2, Endo H3, Numasawa Y4, Tomimitsu H2, Shintani S2.
Journal of the neurological sciences.J Neurol Sci.2015 Jul 15;354(1-2):140-1. doi: 10.1016/j.jns.2015.05.015. Epub 2015 May 21.
PMID 26025748

Development of nodules on the glans penis due to hyaluronic acid filler injection.

Fukuda H1, Endo H1, Katsuzaki J2, Mukai H1.
European journal of dermatology : EJD.Eur J Dermatol.2015 Jun 17. [Epub ahead of print]
PMID 26081014

Radiation sensitivity assay with a panel of patient-derived spheroids of small cell carcinoma of the cervix.

Nakajima A1, Endo H, Okuyama H, Kiyohara Y, Kimura T, Kamiura S, Hiraoka M, Inoue M.
International journal of cancer. Journal international du cancer.Int J Cancer.2015 Jun 15;136(12):2949-60. doi: 10.1002/ijc.29349. Epub 2014 Dec 11.
Small cell carcinoma of the uterine cervix (SCCC) is a rare cancer with a poor prognosis for which no standard treatment exists. Here, we successfully established panels of patient-derived spheroid cultures from six SCCC patient samples by cancer tissue-originated spheroids (CTOS) method. To assess
PMID 25408479

Japanese Journal

中国広西における諸言語の無声側面摩擦音の地理分布と形成過程 (短期研究プロジェクト報告)

遠藤光暁,エンドウミツアキ,Endo Mitsuaki
経済研究 5, 21-30, 20130300-00-00
NAID 120005308416

フレッシャーズセミナー(2012年度前期) (教育の部)

遠藤光暁,エンドウミツアキ,Endo Mitsuaki
青山スタンダード論集 8, 3-14, 20130100-00-00
NAID 120005291033

Highly Oriented Polymer Field-Effect Transistors with High Electrical Stability

Endo Toshiyuki,Nagase Takashi,Kobayashi Takashi,Naito Hiroyoshi
Jpn J Appl Phys 52(12), 121601-121601-4, 2013-12-25
Oriented polymer field-effect transistors (FETs) with a top-gate configuration have been fabricated using amorphous fluoropolymers as gate insulators. The oriented poly(9,9-dioctylfluorene-co-bithioph …
NAID 150000107833