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- Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
- Tan LL, Ahmed SA, Ng SK, Citartan M, Raabe CA, Rozhdestvensky TS, Tang TH.
- Food chemistry. 2020 Mar;309()125654.
- A specialized DNA extraction method and a SYBR Green quantitative polymerase chain reaction (SyG-qPCR) assay were combined to generate a ready-to-use kit for rapid detection of porcine admixtures in processed meat products. Our qPCR assay utilized repetitive LINE-1 elements specific to the genome of
- PMID 31678669
- On transposons and totipotency.
- , T T, M M, M M, .
- Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 2020 Mar;375(1795)20190339.
- Our perception of the role of the previously considered 'selfish' or 'junk' DNA has been dramatically altered in the past 20 years or so. A large proportion of this non-coding part of mammalian genomes is repetitive in nature, classified as either satellites or transposons. While repetitive elements
- PMID 32075562
- Human transposon insertion profiling by sequencing (TIPseq) to map LINE-1 insertions in single cells.
- McKerrow W, Tang Z, Steranka JP, Payer LM, Boeke JD, Keefe D, Fenyö D, Burns KH, Liu C.
- Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 2020 Mar;375(1795)20190335.
- Long interspersed element-1 (LINE-1, L1) sequences, which comprise about 17% of human genome, are the product of one of the most active types of mobile DNAs in modern humans. LINE-1 insertion alleles can cause inherited and de novo genetic diseases, and LINE-1-encoded proteins are highly expressed i
- PMID 32075555
- PCR Detection of DNAs of Animal Origin in Feed by Primers Based on Sequences of Short and Long Interspersed Repetitive Elements
- Tajima Kiyoshi,Enishi Osamu,Amari Masahiro [他],MITSUMORI Makoto,KAJIKAWA Hiroshi,KURIHARA Mitsunori,YANAI Satoshi,MATSUI Hiroki,YASUE Hiroshi,MITSUHASHI Tadayoshi,KAWASHIMA Tomoyuki,MATSUMOTO Mitsuo
- Bioscience, biotechnology, and biochemistry 66(10), 2247-2250, 2002-10-23
- … PCR primers for the detection of materials derived from ruminants, pigs, and chickens were newly designed on the basis of sequences of the Art2 short interspersed repetitive element (SINE), PRE-1 SINE, and CR1 long interspersed repetitive element (LINE), respectively. …
- NAID 110002693546
- Genomic Structure of the Rat and Mouse Histamine N-Methyltransferase Gene
- Kitanaka Nobue,Kitanaka Junichi,Oue Tetsuya [他],TADA Yoshihiko,TANAKA Tatsuya,TAKEMURA Motohiko
- The Japanese journal of pharmacology 88(1), 85-92, 2002-01-01
- … Several long interspersed repetitive elements were located in the 5' flanking region of the rat and mouse HMT gene. …
- NAID 10007944851
- The 3' ends of tRNA-derived short interspersed repetitive elements are derived from the 3' ends of long interspersed repetitive elements.
- This channel contains Biotechnology notes of M.Sc. Biotechnology. If you are a biotech student this channel is going to help you. You can use this notes for scoring good marks in your exam. If you ...
- Long Interspersed Nuclear Element Long interspersed nuclear elements (LINEs) are an ancient feature of around 6kbp long that make up 20% of the genome and contain all the necessary information for self-transposition. From: Companion to Psychiatric Studies (Eighth Edition), 2010
- Long interspersed repetitive elements (LINEs) are transposable elements present in many species. In mammals they are difficult to study because most of them are defective and their transposition frequency is low. The I factor of Drosophila melanogaster ...
- long interspersed nuclear element, long interspersed nucleotide element, long interspersed element, long interspersed repetitive elements, LINEs, long interspersed repeat sequences
- quoted from http://en.wikipedia.org/wiki/Long_interspersed_nucleotide_elements#LINEs
- Long interspersed nuclear elements are a group of genetic elements that are found in large numbers in eukaryotic genomes. They are transcribed (or are the evolutionary remains of what was once transcribed) to an RNA using an RNA polymerase II promoter that resides inside the LINE. LINEs code for the enzyme reverse transcriptase, and many LINEs also code for an endonuclease (e.g. RNase H). The reverse transcriptase has a higher specificity for the LINE RNA than other RNA, and makes a DNA copy of the RNA that can be integrated into the genome at a new site.
- The 5' UTR contains the promoter sequence, while the 3' UTR contains a polyadenylation signal (AATAAA) and a poly-A tail. Because LINEs move by copying themselves (instead of moving, like transposons do), they enlarge the genome. The human genome, for example, contains about 20,000-40,000 LINEs, which is roughly 21% of the genome.