NBT還元試験
WordNet
- undergo a test; "She doesnt test well"
- any standardized procedure for measuring sensitivity or memory or intelligence or aptitude or personality etc; "the test was standardized on a large sample of students" (同)mental test, mental testing, psychometric test
- the act of undergoing testing; "he survived the great test of battle"; "candidates must compete in a trial of skill" (同)trial
- the act of testing something; "in the experimental trials the amount of carbon was measured separately"; "he called each flip of the coin a new trial" (同)trial, run
- a hard outer covering as of some amoebas and sea urchins
- put to the test, as for its quality, or give experimental use to; "This approach has been tried with good results"; "Test this recipe" (同)prove, try, try out, examine, essay
- achieve a certain score or rating on a test; "She tested high on the LSAT and was admitted to all the good law schools"
- determine the presence or properties of (a substance)
- show a certain characteristic when tested; "He tested positive for HIV"
- an examination of the characteristics of something; "there are laboratories for commercial testing"; "it involved testing thousands of children for smallpox"
- the act of subjecting to experimental test in order to determine how well something works; "they agreed to end the testing of atomic weapons"
- a usually soluble substance for staining or coloring e.g. fabrics or hair (同)dyestuff
- color with dye; "Please dye these shoes"
- in or associated with the process of passing from life or ceasing to be; "a dying man"; "his dying wish"; "a dying fire"; "a dying civilization"
- tested and proved useful or correct; "a tested method" (同)tried, well-tried
- tested and proved to be reliable (同)time-tested, tried, tried and true
PrepTutorEJDIC
- (人の能力などの)『試験』,考査,テスト / (物事の)『試験』,検済,試錬,実験《+of+名》 / 化学分析;試薬 / =test match / …‘を'『試験する』,検査する / …‘を'化学分析する / (…の)試験を受ける,試験をする《+for+名》
- (髪・布などの)『染料』 / 染め色,色合い / …‘を'『染める』,‘に'色をつける / 〈布などが〉『染まる』
- dieの現在分詞 / 死にかかっている,ひん死の / 消えかかっている,滅びかけている / 臨終の
UpToDate Contents
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English Journal
- Antifilarial effects of polyphenol rich ethanolic extract from the leaves of Azadirachta indica through molecular and biochemical approaches describing reactive oxygen species (ROS) mediated apoptosis of Setaria cervi.
- Mukherjee N1, Mukherjee S1, Saini P1, Roy P1, Sinha Babu SP2.Author information 1Parasitology Laboratory, Department of Zoology, Centre for Advanced Studies, Visva-Bharati University, Santiniketan 731 235, West Bengal, India.2Parasitology Laboratory, Department of Zoology, Centre for Advanced Studies, Visva-Bharati University, Santiniketan 731 235, West Bengal, India. Electronic address: spsinhababu@gmail.com.AbstractLymphatic filariasis, a global cause of morbidity needs much more attention in developing potent therapeutics that can be effective against both microfilariae (mf) and adults. Efficient botanicals that can induce apoptosis of filarial parasites possibly can provide a direction towards developing new class of antifilarials. In this work we have evaluated the antifilarial efficacy of an optimized polyphenol rich ethanolic extract of Azadirachta indica leaves (EEA). A. indica A. Juss has been widely used in the traditional Indian medicinal system 'Ayurveda' for the treatment of a variety of ailments. A thorough investigation towards biochemical and molecular mechanisms describing ROS mediated apoptosis in Setaria cervi was performed. Motility reduction, MTT reduction assay and dye exclusion test have confirmed the micro- and macrofilaricidal potential of EEA. Alterations were visible in mf and trichrome stained section of EEA-treated adult worms. We have found cellular disturbances in EEA-treated parasites characterized by chromatin condensation, in situ DNA fragmentation and nucleosomal DNA laddering. Depletion in worm GSH level and elevation in parasite GST, SOD, catalase, GPx and superoxide anion indicated the generation of ROS. Our results provided experimental evidence supporting that EEA causes a decreased expression of anti-apoptotic genes and increased pro-apoptotic gene expression at the level of both transcription and translation. Here we are reporting for the first time that antifilarial activity of EEA is mediated by ROS up regulation and apoptosis.
- Experimental parasitology.Exp Parasitol.2014 Jan;136:41-58. doi: 10.1016/j.exppara.2013.11.006. Epub 2013 Nov 23.
- Lymphatic filariasis, a global cause of morbidity needs much more attention in developing potent therapeutics that can be effective against both microfilariae (mf) and adults. Efficient botanicals that can induce apoptosis of filarial parasites possibly can provide a direction towards developing new
- PMID 24275557
- Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils.
- Heroor S, Beknal AK, Mahurkar N.Author information Department of Pharmacognosy and Phytochemistry, HKES's MTR Institute of Pharmaceutical Sciences, Gulbarga, Karnataka, India.AbstractOBJECTIVE: To evaluate the immunomodulatory activity of methanolic extracts of fruit and bark of Ficus glomerata Roxb. on cyclophosphamide-induced myelosuppression in mice and the phagocytic effect on human neutrophils.
- Indian journal of pharmacology.Indian J Pharmacol.2013 Mar-Apr;45(2):130-5. doi: 10.4103/0253-7613.108287.
- OBJECTIVE: To evaluate the immunomodulatory activity of methanolic extracts of fruit and bark of Ficus glomerata Roxb. on cyclophosphamide-induced myelosuppression in mice and the phagocytic effect on human neutrophils.MATERIALS AND METHODS: Methanolic extracts of fruits and bark of Ficus glomerata
- PMID 23716887
- A new look on protein-polyphenol complexation during honey storage: is this a random or organized event with the help of dirigent-like proteins?
- Brudzynski K, Sjaarda C, Maldonado-Alvarez L.Author information Department of Drug Discovery and Development, Bee-Biomedicals Inc., St. Catharines, Ontario, Canada ; Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.AbstractHoney storage initiates melanoidin formation that involves a cascade of seemingly unguided redox reactions between amino acids/proteins, reducing sugars and polyphenols. In the process, high molecular weight protein-polyphenol complexes are formed, but the mechanism involved remains unknown. The objective of this study was twofold: to determine quantitative and qualitative changes in proteins in honeys stored for prolonged times and in different temperatures and to relate these changes to the formation of protein-polyphenol complexes. Six -month storage decreased the protein content by 46.7% in all tested honeys (t-test, p<0.002) with the rapid reduction occurring during the first three month. The changes in protein levels coincided with alterations in molecular size and net charge of proteins on SDS -PAGE. Electro-blotted proteins reacted with a quinone-specific nitro blue tetrazolium (NBT) on nitrocellulose membranes indicating that quinones derived from oxidized polyphenols formed covalent bonds with proteins. Protein-polyphenol complexes isolated by size-exclusion chromatography differed in size and stoichiometry and fall into two categories: (a) high molecular weight complexes (230-180 kDa) enriched in proteins but possessing a limited reducing activity toward the NBT and (b) lower molecular size complexes (110-85 kDa) enriched in polyphenols but strongly reducing the dye. The variable stoichiometry suggest that the large, "protein-type" complexes were formed by protein cross-linking, while in the smaller, "polyphenol-type" complexes polyphenols were first polymerized prior to protein binding. Quinones preferentially bound a 31 kDa protein which, by the electrospray quadrupole time of flight mass spectrometry (ESI-Qtof-MS) analysis, showed homology to dirigent-like proteins known for assisting in radical coupling and polymerization of phenolic compounds. These findings provide a new look on protein-polyphenol interaction in honey where the reaction of quinones with proteins and polyphenols could possibly be under assumed guidance of dirigent proteins.
- PloS one.PLoS One.2013 Aug 30;8(8):e72897. doi: 10.1371/journal.pone.0072897. eCollection 2013.
- Honey storage initiates melanoidin formation that involves a cascade of seemingly unguided redox reactions between amino acids/proteins, reducing sugars and polyphenols. In the process, high molecular weight protein-polyphenol complexes are formed, but the mechanism involved remains unknown. The obj
- PMID 24023654
Japanese Journal
- ウサギ下顎骨感染モデルの多形核白血球に関する実験的研究
- 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases 72(5), 526-535, 1998-05-20
- NAID 10008718047
- ウサギ下顎骨感染モデルの多形核白血球に関する実験的研究
- 新生児, 低出生体重児のNitroblue-tetrazolium (NBT) 色素還元試験:第1編NBT色素還元試験の基礎的条件設定
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- 英
- nitroblue tetrazolium dye test NBT test, nitroblue tetrazolium test NBT dye test
- 同
- NBT色素還元能検査、ニトロブルー・テトラゾリウム試験、NBT還元能試験
- 関
- 白血球、ニトロブルーテトラゾリウム nitroblue tetrazolium、NBT還元能
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- 関
- assessment、data quality、exam、examination、examine、experimental design、matched group、measurement、research design、scoring method、test、trial
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染める
- 関
- coloring agent、dye stuff、pigment、stain、tissue stain
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- 関
- moribund