- 関
- ethanol precipitation、EtOH ppt
WordNet
- separate as a fine suspension of solid particles
- a precipitated solid substance in suspension or after settling or filtering
- fall from clouds; "rain, snow and sleet were falling"; "Vesuvius precipitated its fiery, destructive rage on Herculaneum" (同)come_down, fall
- bring about abruptly; "The crisis precipitated by Russias revolution"
- fall vertically, sharply, or headlong; "Our economy precipitated into complete ruin"
- hurl or throw violently; "The bridge broke and precipitated the train into the river below"
- a message whose ingenuity or verbal skill or incongruity has the power to evoke laughter (同)humor, humour, witticism, wittiness
- bringing on suddenly or abruptly; "the completion of the railroad was the precipitating cause in the extinction of waterborne commerce"
PrepTutorEJDIC
- …‘を'思いがけなく引き起こす,‘の'致来を早める / (…の中に)…‘を'投げ落とす,(…の状態に)…‘を'陥らせる《+『名』+『into』+『名』》 / 〈流動体〉‘を'沈殿させる / (雨・雪・霧などに)〈水蒸気〉‘を'凝結させる / 〈水蒸気が〉凝結して雨(雪など)となる / 〈流動体が〉沈殿して固体になる / 大急ぎの;まっしぐらの / (化学で)沈殿物
- 《随伴・協調》…『と』,と共に,といっしょに / 《所有・所持》…『を持っている』,がある,の付いた / 《道具・手段》…『で』,を使って / 《財料・供給物》…『で』,を与えて / …を支持して,に賛成して / …『に反対(敵対)して』,を相手として / …『と同時に』,と同様に / …『に比べて』,に比例して / 《様態》《名詞を伴い副詞句として》『…で』,を示して,をもって / 《付帯状競》…『して』,しながら / 《譲歩》…がありながら,にもかかわらず / 『…が原因で』,のために,…で / …に任せて,のもとにあずけて,のところに / …に関して,について / …から離れて / 《連結・混合》…と / 《副詞と共に命令文で》
- 知る(know)
- 〈U〉『機知』,機転,ウィット / 〈C〉『機知に富む人』,機転のきく人 / 《複数形で;単数扱い》『理解(表現)力』,知性 / 《複数形で》(物事を見抜いたりもくろんだりする)才覚,才知 / 《複数形で;単数扱い》正気
- =alcohol
UpToDate Contents
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English Journal
- A competent extraction method of plant proteins for 2-D gel electrophoresis.
- Ashoub A, Berberich T, Beckhaus T, Bruggemann W.SourceBiodiversity and Climate Research Centre (BiK-F), Frankfurt am Main, Germany; Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt. ashoub@em.uni-frankfurt.de.
- Electrophoresis.Electrophoresis.2011 Oct 14. doi: 10.1002/elps.201100150. [Epub ahead of print]
- The efficient extraction of high-quality proteins is a key factor for a successful proteomic analysis approach. In the method suggested here, absolute ethanol containing 10?mM DTT was used to precipitate the proteins in plant tissue homogenates followed by their resuspension in a urea-/thiourea- a
- PMID 21997354
- Drug specificity in extended access cocaine and heroin self-administration.
- Lenoir M, Guillem K, Koob GF, Ahmed SH.SourceUniversite de Bordeaux, Institut des Maladies Neurodegeneratives, France CNRS, Institut des Maladies Neurodegeneratives, France Behavioral Neuroscience Branch, National Institute on Drug Abuse-Intramural Research Program, NIH, Baltimore, MD, USA Committee on the Neurobiology of Addictive Disorder, The Scripps Research Institute, La Jolla, CA, USA.
- Addiction biology.Addict Biol.2011 Oct 13. doi: 10.1111/j.1369-1600.2011.00385.x. [Epub ahead of print]
- Increased drug availability can precipitate a rapid escalation of drug consumption in both vulnerable humans and laboratory animals. Drug intake escalation is observed across a broad spectrum of drugs of abuse, including stimulants, opiates, ethanol and phencyclidine. Whether and to what extent the
- PMID 21995515
Japanese Journal
- Abe Kazuhiro,Higuchi Tsuyoshi,Mizunuma Hideki,Kakizaki Ikuko,Endo Masahiko,Suto Shinichirou
- 弘前醫學 61(2/3/4), 97-103, 2011
- … The aim of study is to analyze functional sugar chains, glycosaminoglycans (GAGs) in urine frompostmenopausal women with osteoporosis. … The urine was concentrated by dialysis and evaporation, thenprecipitated with ethanol. … The precipitate was then reconstituted into NaCl solution, precipitated again by adding 10% cetylpyridinium chloride and the precipitation was used as complex GAGs fraction. …
- NAID 110008425033
- Preparation of Optically Active Allothreonine by Separating from a Diastereoisomeric Mixture with Threonine
- YAJIMA Tatsuo,ICHIMURA Serina,HORII Shirabe,SHIRAIWA Tadashi
- Bioscience, biotechnology, and biochemistry 74(10), 2106-2109, 2010-10-23
- … A mixture of N-acetyl-D-allothreonine (Ac-D-aThr) and N-acetyl-L-threonine (Ac-L-Thr) was converted to a mixture of their ammonium salts and then treated with ethanol to precipitate ammonium N-acetyl-L-threoninate (Ac-L-Thr·NH3) as the less-soluble diastereoisomeric salt. …
- NAID 10027560788
Related Links
- ... precipitation. From Wikipedia, the free encyclopedia. Jump to: navigation, search. Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions . ...
Related Pictures
★リンクテーブル★
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- 英
- ethanol precipitation、EtOH ppt、precipitate with ethanol
- 関
- エタ沈、エタノール沈澱
伝統的なプロトコール
- x ul of DNA soln.
- add 1/10 volume of 3M NaOAc
- add 2.5 volume of 100% EtOH
- mix well
- cfg. max speed for 20? min at 4C ?
- ppt
- add 100? ul of 70% EtOH
- cfg. max speed for 10? min at 4C ?
- ppt
- dry up
- add 20 ul of TE and dissolve
エタノール沈殿における3M NaOAcのpH
- (参考1)
- pH5.2: RNA
- pH7.5: DNA
- pH5.2をDNAに使っても問題ないという噂だが。
参考
- http://oshiete.goo.ne.jp/qa/6065293.html
[★]
- 関
- EtOH ppt、precipitate with ethanol
[★]
エタノール沈殿
- 関
- ethanol precipitation、precipitate with ethanol
[★]
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- the combined negative inotropic and chronotropic effects may precipitate heart failure.
- we report the first case of myocardial infarction in a hemophilia patient which was not directly precipitated by infusion of a clotting factor.
- 真っ逆さまの。突進する
- 大あわての、軽率な。(十分考えず)突然の
- 関
- challenge、elicit、elicitation、evocation、evoke、induce、induction、inductive、precipitation、provocation、provoke、sediment、sedimentation、spark