エタノール沈殿
- 関
- ethanol precipitation、precipitate with ethanol
UpToDate Contents
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English Journal
- Treatment with bisphenol A and methoxychlor results in the growth of human breast cancer cells and alteration of the expression of cell cycle-related genes, cyclin D1 and p21, via an estrogen receptor-dependent signaling pathway.
- Lee HR1, Hwang KA, Park MA, Yi BR, Jeung EB, Choi KC.
- International journal of molecular medicine.Int J Mol Med.2012 May;29(5):883-90. doi: 10.3892/ijmm.2012.903. Epub 2012 Feb 3.
- Various endocrine disrupting chemicals (EDCs) are exogenous compounds found in the environment and have the potential to interfere with the endocrine system and hormonal regulation. Among EDCs, bisphenol A (BPA) and 1,1,1-trichloro-2,2-bis(4-methoxyphenol)-ethane
- PMID 22307313
- Ethanol impairs estrogen receptor signaling resulting in accelerated activation of senescence pathways, whereas estradiol attenuates the effects of ethanol in osteoblasts.
- Chen JR1, Lazarenko OP, Haley RL, Blackburn ML, Badger TM, Ronis MJ.
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.J Bone Miner Res.2009 Feb;24(2):221-30. doi: 10.1359/jbmr.081011.
- Epidemiological and animal studies have suggested that chronic alcohol consumption is a major risk factor for osteoporosis. Using bone from cycling female rats infused chronically with ethanol (EtOH) in vivo and osteoblastic cells in vitro, we found that EtOH significantly increased estrogen recepto
- PMID 18847333
- Comparison of two rapid cortisol radioimmunoassays for use in the fetal sheep.
- Magyar DM, Elsner CW, Nathanielsz PW, Lowe KC, Buster JE.
- Steroids.Steroids.1979 Jul;34(1):111-9.
- Cortisol radioimmunoassays (RIA's) utilizing highly specific antisera combined with a simple ethanol protein precipitation procedure (ETOH-PPT) are widely utilized to measure cortisol in human plasma. This same type of RIA has been assumed specific for measurement of cortisol in the plasma of severa
- PMID 483333
Japanese Journal
- Carcinostatic Effect of Extracts from Human Gastric Cancer
- 船岡 宏明,江崎 治夫,荒木 文雄,杉原 芳夫
- Journal of radiation research 5(3-4,3,4), 159-165, 1964-09
- … Saline extracts from human gastric cancer divided into two parts, one of which was irradiated by X-ray of 2000 r, before homogenization, were fractionated with EtOH, in which S_1 was 30% vol. ppt. … , S_2 was 30-70% vol. ppt. … Further, S_1 and S_2 were fractionated with (NH_4)_2SO_4 into P_1 (30% sat. ppt.), P_2 (30-50% sat. ppt.), P_3 (50-7O% sat. ppt.), and P_4 (supernatant). …
- NAID 110002322897
- Urinary Gonadotropins from Goats : II. Purification by Liquid Chromotography
- 今井 清,長谷川 靖彦,中条 誠一
- 日本畜産学会報 33(1), 59-64, 1962
- … The precipitate was named (GA+GB) EtOH ppt. … and stored in a desiccator until it was subjected to biological assay.When (GA+GB) EtOH ppt. …
- NAID 130000740454
Related Links
- >>POP辞書.com PC版 >>英語ニュースで練習 >>携帯ウィキペディア検索 結果: EtOH ppt: 1)《略》エタノール沈殿 EtOH: 1)《略》エタノール Ethiopia: 1)エチオピア(アフリカ 東部 の 国;首都 はAddis Ababa;旧称 はAbyssinia) ...
- Common methods of experiment of molecular biology ... '01-9-7-Fri. Experiment Basic method Ethanol precipitation (EtOH ppt.) P/C -> C -> etc. Add 100% cold EtOH ( 2 × vol.) Add NaOAc (sodium acetate) (1/10 × vol.)
★リンクテーブル★
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- 英
- ethanol precipitation、EtOH ppt、precipitate with ethanol
- 関
- エタ沈、エタノール沈澱
伝統的なプロトコール
- x ul of DNA soln.
- add 1/10 volume of 3M NaOAc
- add 2.5 volume of 100% EtOH
- mix well
- cfg. max speed for 20? min at 4C ?
- ppt
- add 100? ul of 70% EtOH
- cfg. max speed for 10? min at 4C ?
- ppt
- dry up
- add 20 ul of TE and dissolve
エタノール沈殿における3M NaOAcのpH
- (参考1)
- pH5.2: RNA
- pH7.5: DNA
- pH5.2をDNAに使っても問題ないという噂だが。
参考
- http://oshiete.goo.ne.jp/qa/6065293.html
[★]
- 関
- EtOH ppt、precipitate with ethanol
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- 関
- ethanol precipitation、EtOH ppt
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- 10の-12乗
- 関
- pico
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- 関
- ethanol
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プロトロンビン時間