- 関
- co-purify、copurification、copurify
WordNet
- a ceremonial cleansing from defilement or uncleanness by the performance of appropriate rites (同)purgation
- the act of cleaning by getting rid of impurities
- the act of purging of sin or guilt; moral or spiritual cleansing; "purification through repentance"
- major food fish of Arctic and cold-temperate waters (同)codfish
- lean white flesh of important North Atlantic food fish; usually baked or poached (同)codfish
- lettuce with long dark-green leaves in a loosely packed elongated head (同)cos_lettuce, romaine, romaine lettuce
PrepTutorEJDIC
- 清めること,浄化
- =codfish 1
- 〈人〉'を'ばかにする
- cosine
- =because
- Colorado
- cobaltの化学記号
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/07/31 20:32:21」(JST)
[Wiki en表示]
Copurification in a chemical or biochemical context is the physical separation by chromatography or other purification technique of two or more substances of interest from other contaminating substances. For substances to co-purify usually implies that these substances attract each other to form a non-covalent complex such as in a protein complex.[1]
However, when fractionating mixtures, especially mixtures containing large numbers of components (for example a cell lysate), it is possible by chance that some components may copurify even though they don't form complexes. In this context the term copurification is sometimes used to denote when two biochemical activities or some other property are isolated together after purification but it is not certain if the sample has been purified to homogeneity (i.e., contains only one molecular species or one molecular complex). Hence these activities or properties are likely but not guaranteed to reside on the same molecule or in the same molecular complex.
Applications
Copurification procedures, such as co-immunoprecipitation, are commonly used to analyze interactions between proteins.[2] Copurification is one method used to map the interactome of living organisms.[3]
References
- ^ Golemis, Erica (2002). Protein-protein interactions: a molecular cloning manual. Plainview, N.Y: Cold Spring Harbor Laboratory Press. ISBN 0-87969-628-1.
- ^ Schon, Eric A.; Pon, Liza A. (2001). Mitochondria. Methods in Cell Biology 65. Boston: Academic Press. pp. 218–219. ISBN 0-12-544169-X.
- ^ Stumpf MP, Thorne T, de Silva E, Stewart R, An HJ, Lappe M, Wiuf C (May 2008). "Estimating the size of the human interactome". Proc. Natl. Acad. Sci. U.S.A. 105 (19): 6959–64. Bibcode:2008PNAS..105.6959S. doi:10.1073/pnas.0708078105. PMC 2383957. PMID 18474861.
UpToDate Contents
全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.
English Journal
- Generation of Recombinant Polioviruses Harboring RNA Affinity Tags in the 5' and 3' Noncoding Regions of Genomic RNAs.
- Flather D1, Cathcart AL2,3, Cruz C4, Baggs E5, Ngo T6, Gershon PD7, Semler BL8.
- Viruses.Viruses.2016 Feb 4;8(2). pii: E39. doi: 10.3390/v8020039.
- Despite being intensely studied for more than 50 years, a complete understanding of the enterovirus replication cycle remains elusive. Specifically, only a handful of cellular proteins have been shown to be involved in the RNA replication cycle of these viruses. In an effort to isolate and identify
- PMID 26861382
- Up-regulation of cytosolic tryparedoxin in Amp B resistant isolates of Leishmania donovani and its interaction with cytosolic tryparedoxin peroxidase.
- Suman SS1, Equbal A1, Zaidi A1, Ansari MY2, Singh KP1, Singh K3, Purkait B4, Sahoo GC5, Bimal S6, Das P4, Ali V7.
- Biochimie.Biochimie.2016 Feb;121:312-25. doi: 10.1016/j.biochi.2015.12.017. Epub 2015 Dec 30.
- Leishmania is a unicellular protozoan parasite which causes leishmaniasis, a neglected tropical disease. It possess a unique thiol metabolism comprising of several proteins among which, tryparedoxin (cTXN) and tryparedoxin peroxidase (cTXNPx), function in concert as oxidoreductases, utilizing trypan
- PMID 26743980
- Ubiquitin-Activated Interaction Traps (UBAITs) identify E3 ligase binding partners.
- O'Connor HF1, Lyon N1, Leung JW1, Agarwal P1, Swaim CD1, Miller KM1, Huibregtse JM2.
- EMBO reports.EMBO Rep.2015 Dec;16(12):1699-712. doi: 10.15252/embr.201540620. Epub 2015 Oct 27.
- We describe a new class of reagents for identifying substrates, adaptors, and regulators of HECT and RING E3s. UBAITs (Ubiquitin-Activated Interaction Traps) are E3-ubiquitin fusion proteins and, in an E1- and E2-dependent manner, the C-terminal ubiquitin moiety forms an amide linkage to proteins th
- PMID 26508657
Japanese Journal
- Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis
- Ushimaru Yuji,Konno Aru,Kaizu Maiko [他],Ogawa Kazuo,Satoh Nori,Inaba Kazuo,稲葉 一男
- Zoological science 23(8), 679-687, 2006-08-25
- … Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. … This co-purification was not affected by the absence of Mg2+ in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. …
- NAID 110006151750
- NADP-malic Enzyme and Hsp70 : Co-purification of Both Proteins and Modification of NADP-malic Enzyme Properties by Association with Hsp70
- Lara Maria V.,Drincovich Maria F.,Muller Gabriela L. [他],MAURINO Veronica G.,ANDREO Carlos S.
- Plant and cell physiology 46(6), 997-1006, 2005-06-01
- The flexibility of the Multi-Link (ML) PENTA stent with platform 0.09 to 0.12-mm-thick struts and 12% to 16% metal/artery coverage was improved to facilitate safe delivery in complex coronary lesions. …
- NAID 10016454964
- A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase
- UCHIDA H
- Nucleosides Nucleotides Nucleic Acids 20, 1647-1654, 2001
- NAID 30002042727
Related Links
- メディア掲載レビューL`Arc~en~Cielのドラマー、yukihiroのソロ・プロジェクト、acid androidの、セカンド・アルバム。2006年4月発表の先行シングル「let`s dance」を含む楽曲を収録。
- Purification Products Limited are a world class manufacturer of filtration Products used in precision industries around the globe. The Company Please note that Purification Products has ceased production. For textile based activated ...
Related Pictures
★リンクテーブル★
[★]
- 関
- co-purification、co-purify、copurification
[★]
- 英
- copurification、co-purification、copurify、co-purify
- 関
- 共精製
[★]
- 関
- co-purification、co-purify、copurify
[★]
- 関
- co-purification、copurification、copurify
[★]
- 関
- cleanup、purify、refine、refinement
[★]
[★]
タラ
- 関
- Gadiformes
[★]
コバルト cobalt
[★]