copurification

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co-purification、co-purify、copurify

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/12/22 20:38:27」(JST)

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Copurification in a chemical or biochemical context is the physical separation by chromatography or other purification technique of two or more substances of interest from other contaminating substances. For substances to co-purify usually implies that these substances attract each other to form a non-covalent complex such as in a protein complex.

However when fractionating mixtures, especially mixtures containing large numbers of components (for example a cell lysate), it is possible by chance that some components may copurify even though they don't form complexes. In this context the term copurification is sometimes used to denote when two biochemical activities or some other property are isolated together after purification but it is not certain if the sample has been purified to homogeneity (i.e., contains only one molecular species or one molecular complex). Hence these activities or properties are likely but not guaranteed to reside on the same molecule or in the same molecular complex.

Applications

Copurification procedures, such as co-immunoprecipitation, are commonly used to analyze interactions between proteins. Copurification is one method used to map the interactome of living organisms.

References

UpToDate Contents

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• 1. 血漿分画製剤および組換えDNA産生凝固因子 plasma derivatives and recombinant dna produced coagulation factors
• 2. 血友病の治療 treatment of hemophilia

English Journal

• Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.

• Fukumura T1, Makino F1, Dietsche T2, Kinoshita M1, Kato T1, Wagner S2,3, Namba K1,4, Imada K5, Minamino T1.
• PLoS biology.PLoS Biol.2017 Aug 3;15(8):e2002281. doi: 10.1371/journal.pbio.2002281. eCollection 2017 Aug.
• PMID 28771466

• Signal Peptide Hydrophobicity Modulates Interaction with the Twin-Arginine Translocase.

• Huang Q1, Palmer T2.
• mBio.MBio.2017 Aug 1;8(4). pii: e00909-17. doi: 10.1128/mBio.00909-17.
• PMID 28765221

• The sole LSm complex in Cyanidioschyzon merolae associates with pre-mRNA splicing and mRNA degradation factors.

• Reimer KA1, Stark MR1, Aguilar LC2, Stark SR1, Burke RD3, Moore J4, Fahlman RP4,5, Yip CK6, Kuroiwa H7, Oeffinger M2,8, Rader SD1.
• RNA (New York, N.Y.).RNA.2017 Jun;23(6):952-967. doi: 10.1261/rna.058487.116. Epub 2017 Mar 21.
• PMID 28325844

Japanese Journal

• Development of Series of Gateway Binary Vectors, pGWBs, for Realizing Efficient Construction of Fusion Genes for Plant Transformation(PLANT BIOTECHNOLOGY)

• Journal of bioscience and bioengineering 104(1), 34-41, 2007-07-25
• NAID 110006368805

• Copurification of casein kinase II with transcription factor ATF/E4TF3

• Nucleic Acids Res. 24, 876-884, 1996
• NAID 30018406199

• Copurification of glcosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1- phosphate uridyltransferase activities of Escherichia coli: Characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis

• J. Bacteriol. 176, 5788-5795, 1994
• NAID 80007810103

Related Links

• copurification.org

copurification.org

• What does copurification mean? - Definitions.net

Definition of copurification in the Definitions.net dictionary. Meaning of copurification. What does copurification mean? Information and translations of copurification in the most comprehensive dictionary definitions resource on the web.

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リンク元	「copurify」「同時精製」「co-purify」「co-purification」「共精製」

「copurify」

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co-purification、co-purify、copurification

「同時精製」

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英

copurification、co-purification、copurify、co-purify

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共精製

「co-purify」

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• 同時精製する

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co-purification、copurification、copurify

「co-purification」

　　[★]

• 同時精製

関

co-purify、copurification、copurify

「共精製」

　　[★]

英

copurification、copurify

関

同時精製