- 関
- co-purification、co-purify、copurify
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2014/12/22 20:38:27」(JST)
[Wiki en表示]
Copurification in a chemical or biochemical context is the physical separation by chromatography or other purification technique of two or more substances of interest from other contaminating substances. For substances to co-purify usually implies that these substances attract each other to form a non-covalent complex such as in a protein complex.[1]
However when fractionating mixtures, especially mixtures containing large numbers of components (for example a cell lysate), it is possible by chance that some components may copurify even though they don't form complexes. In this context the term copurification is sometimes used to denote when two biochemical activities or some other property are isolated together after purification but it is not certain if the sample has been purified to homogeneity (i.e., contains only one molecular species or one molecular complex). Hence these activities or properties are likely but not guaranteed to reside on the same molecule or in the same molecular complex.
Applications
Copurification procedures, such as co-immunoprecipitation, are commonly used to analyze interactions between proteins.[2] Copurification is one method used to map the interactome of living organisms.[3]
References
- ^ Golemis, Erica (2002). Protein-protein interactions: a molecular cloning manual. Plainview, N.Y: Cold Spring Harbor Laboratory Press. ISBN 0-87969-628-1.
- ^ Schon, Eric A.; Pon, Liza A. (2001). Mitochondria. Methods in Cell Biology 65. Boston: Academic Press. pp. 218–219. ISBN 0-12-544169-X.
- ^ Stumpf MP, Thorne T, de Silva E, Stewart R, An HJ, Lappe M, Wiuf C (May 2008). "Estimating the size of the human interactome". Proc. Natl. Acad. Sci. U.S.A. 105 (19): 6959–64. Bibcode:2008PNAS..105.6959S. doi:10.1073/pnas.0708078105. PMC 2383957. PMID 18474861.
UpToDate Contents
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English Journal
- Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.
- Fukumura T1, Makino F1, Dietsche T2, Kinoshita M1, Kato T1, Wagner S2,3, Namba K1,4, Imada K5, Minamino T1.
- PLoS biology.PLoS Biol.2017 Aug 3;15(8):e2002281. doi: 10.1371/journal.pbio.2002281. eCollection 2017 Aug.
- PMID 28771466
- Signal Peptide Hydrophobicity Modulates Interaction with the Twin-Arginine Translocase.
- Huang Q1, Palmer T2.
- mBio.MBio.2017 Aug 1;8(4). pii: e00909-17. doi: 10.1128/mBio.00909-17.
- PMID 28765221
- The sole LSm complex in Cyanidioschyzon merolae associates with pre-mRNA splicing and mRNA degradation factors.
- Reimer KA1, Stark MR1, Aguilar LC2, Stark SR1, Burke RD3, Moore J4, Fahlman RP4,5, Yip CK6, Kuroiwa H7, Oeffinger M2,8, Rader SD1.
- RNA (New York, N.Y.).RNA.2017 Jun;23(6):952-967. doi: 10.1261/rna.058487.116. Epub 2017 Mar 21.
- PMID 28325844
Japanese Journal
- Development of Series of Gateway Binary Vectors, pGWBs, for Realizing Efficient Construction of Fusion Genes for Plant Transformation(PLANT BIOTECHNOLOGY)
- Copurification of casein kinase II with transcription factor ATF/E4TF3
- Copurification of glcosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1- phosphate uridyltransferase activities of Escherichia coli: Characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis
Related Links
- copurification.org
- Definition of copurification in the Definitions.net dictionary. Meaning of copurification. What does copurification mean? Information and translations of copurification in the most comprehensive dictionary definitions resource on the web.
Related Pictures
![Copurification de protéines Séparation](https://1mg.info/0/5/1/2956.jpg)
![Rac1 is deactivated at integrin activation](https://1mg.info/0/5/1/2957.jpg)
![méthodologies : Copurification de](https://1mg.info/0/5/1/2958.jpg)
![Figure 2. Basic MasterPure™ Complete Kit](https://1mg.info/0/5/1/2959.jpg)
![Protein Complex Analysis combining a one](https://1mg.info/0/5/1/2960.jpg)
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