WordNet
- intend (something) to move towards a certain goal; "He aimed his fists towards his opponents face"; "criticism directed at her superior"; "direct your anger towards others, not towards yourself" (同)aim, place, direct, point
- sports equipment consisting of an object set up for a marksman or archer to aim at (同)butt
- a reference point to shoot at; "his arrow hit the mark" (同)mark
- the location of the target that is to be hit (同)target area
- the 4th letter of the Roman alphabet (同)d
PrepTutorEJDIC
- (鉄砲などの)『標的』,的,攻撃目標 / (批評・軽べつなどの)的,種《+of+名》 / (行為などの)目標,到達目標
- deuteriumの化学記号
- deoxyribonucleic acidディオキシリボ核酸
UpToDate Contents
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English Journal
- Naked eye detection of trace cancer biomarkers based on biobarcode and enzyme-assisted DNA recycling hybrid amplifications.
- Zhou W, Su J, Chai Y, Yuan R, Xiang Y.Author information Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.AbstractNaked eye-based detection has received increasing research interest due to the simplicity nature of this type of assay. However, improving the sensitivity of the naked eye detection method for the monitoring of trace amount of target molecules remains a major challenge. Herein, we describe a biobarcode and an enzyme-assisted DNA recycling hybrid amplification strategy for naked eye detection of sub-picomolar carcinoembryonic antigen (CEA), a cancer biomarker. The presence of CEA and the corresponding antibodies results in the formation of immunocomplexes and the capture of the biobarcodes in a microplate. The massive barcode DNAs released from the biobarcodes hybridize with the G-quadruplex inactive hairpin DNA probes and form catalytic nicking sites for N.BstNBI endonuclease, which cleaves the barcode DNA/hairpin partial dsDNA, releases the G-quadruplex active sequences and recycles the barcode DNA. Due to the barcode DNA recycling process, numerous G-quadruplex active sequences are generated and associate with hemin to form peroxidase mimicking enzymes, which convert colorless ABTS(2-) to green color intensified ABTS(•-) to achieve naked eye detection of CEA down to 0.025ngmL(-1) (0.14pM). The naked eye detection strategy reported herein can be applied also to complicated serum sample matrix, making this approach hold great promise for point-of-care diagnostic applications.
- Biosensors & bioelectronics.Biosens Bioelectron.2014 Mar 15;53:494-8. doi: 10.1016/j.bios.2013.10.020. Epub 2013 Oct 24.
- Naked eye-based detection has received increasing research interest due to the simplicity nature of this type of assay. However, improving the sensitivity of the naked eye detection method for the monitoring of trace amount of target molecules remains a major challenge. Herein, we describe a biobarc
- PMID 24211463
- A label-free and PCR-free electrochemical assay for multiplexed microRNA profiles by ligase chain reaction coupling with quantum dots barcodes.
- Zhu W, Su X, Gao X, Dai Z, Zou X.Author information School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China; College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.AbstractThe profiling of microRNAs (miRNAs) is greatly significant for cellular events or disease diagnosis. Electrochemical methods for miRNAs analysis mostly can only measure one kind of miRNA, which is unambiguous to indicate the disease type and state. Here a label-free and PCR-free electrochemical method is presented for multiplexed evaluation of miRNAs in a single-tube experiment. The method is based on the combination of the high base-mismatch selectivity of ligase chain reaction (LCR) and the remarkable voltammetric signature of electrochemical QDs barcodes. Two reporting probes of RP1 and RP2 were labeled with PbS and CdS quantum dots (QDs) to prepare PbS-RP1 and CdS-RP2 conjugates, and two capture probes of CP1 and CP2 were co-immobilized on magnetic beads (MBs) to fabricate MB-CP1CP2 conjugate. The miRNAs samples were simply incubated with MB-CP1CP2, PbS-RP1, and CdS-RP2 conjugates, and then added with T4 DNA ligase. After release of the disjoined QDs barcodes from the MB-conjugates, two target miRNAs of miR-155 and miR-27b were simultaneously detected by square wave voltammetry with linear ranges of 50fM-30pM and 50fM-1050pM, and limits of detection (LODs) of 12fM and 31fM (S/N=3). The method fulfilled the assay in less than 70min, and showed acceptable testing recoveries for the determination of miRNAs in biological matrix. Currently there are rare reports about electrochemical multiplexed quantification of miRNAs. The method is likely to provide a new platform for identification of multiple miRNAs in a simple way.
- Biosensors & bioelectronics.Biosens Bioelectron.2014 Mar 15;53:414-9. doi: 10.1016/j.bios.2013.10.023. Epub 2013 Oct 24.
- The profiling of microRNAs (miRNAs) is greatly significant for cellular events or disease diagnosis. Electrochemical methods for miRNAs analysis mostly can only measure one kind of miRNA, which is unambiguous to indicate the disease type and state. Here a label-free and PCR-free electrochemical meth
- PMID 24201005
- Metal sulfide-functionalized DNA concatamer for ultrasensitive electronic monitoring of ATP using a programmable capillary-based aptasensor.
- Liu B, Zhang B, Chen G, Yang H, Tang D.Author information Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education & Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.AbstractA new flow-through electrochemical aptasensor was designed for ultrasensitive monitoring of adenosine triphosphate (ATP) by coupling microvalve-programmable capillary column with CdS-functionalized DNA concatamer for signal amplification. Initially, a layer of primary DNA-conjugated polyacrylamide hydrogel was covalently linked onto the internal surface of capillary column, and then an automated sequenctial injection format with a syringe pump was employed for development of the programmable capillary-based aptasensor. In the presence of target DNA aptamer, the immobilized primary DNA hybridized with partial bases of the aptamer. The excess aptamer fregment could trigger the formation of DNA concatamer between auxiliary DNA1 and CdS-labeled auxiliary DNA2. Upon target ATP introduction, a specific ATP-aptamer reaction was excuated, thereby resulting in the release of CdS-functionalized DNA concatamer from the capillary. Subsenquent anodic stripping voltammetric detection of cadmium released under acidic conditions from the released CdS nanoparticles could be conducted in a homemade detection cell. Under optimal conditions, the dynamic concentration range spanned from 0.1pM to 10nM ATP with a detection limit of 0.06pM ATP. The electrochemical aptasensor showed good reproducibility, selectivity, and stability. In addition, the methodology was evaluated for the analysis of ATP spiked serum samples, and the recoveries was 81-140%.
- Biosensors & bioelectronics.Biosens Bioelectron.2014 Mar 15;53:390-8. doi: 10.1016/j.bios.2013.10.022. Epub 2013 Oct 24.
- A new flow-through electrochemical aptasensor was designed for ultrasensitive monitoring of adenosine triphosphate (ATP) by coupling microvalve-programmable capillary column with CdS-functionalized DNA concatamer for signal amplification. Initially, a layer of primary DNA-conjugated polyacrylamide h
- PMID 24201002
Japanese Journal
- Genetic Analysis of T Cell Lymphomas in Carbon Ion-Irradiated Mice Reveals Frequent Interstitial Chromosome Deletions: Implications for Second Cancer Induction in Normal Tissues During Carbon Ion Radiotherapy
- Benjamin Blyth,柿沼 志津子,砂押 正章,甘崎 佳子,坂入 しのぶ,小川 佳那依,白神 綾奈,尚 奕,鶴岡 千鶴,西村 まゆみ,島田 義也
- PLOS ONE, 2015-06
- … Monitoring mice exposed to carbon ion radiotherapy provides an indirect method to evaluate the potential for second cancer induction innormal tissues outside the radiotherapy target volume, since such estimates are not yet possible from historical patient data. …
- NAID 120005622701
- Single-crystal gallium oxide-based biomolecular modified diode for nucleic acid sensing
- Rahman Tanzilur,Masui Takekazu,Ichiki Takanori
- Jpn. J. Appl. Phys. 54(4S), 04DL08, 2015-03-26
- … The surface of a β-Ga<inf>2</inf>O<inf>3</inf>substrate was silanized with 3-aminopropyl-triethoxysilane (APTES) and modified with N-succinimidyl-6-maleimidylhexanoate (EMCS) to immobilize the capture probe DNA (thiol-terminated single-stranded DNA). …
- NAID 150000110410
- A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector.
- Ebina Hirotaka,Kanemura Yuka,Misawa Naoko,Sakuma Tetsushi,Kobayashi Tomoko,Yamamoto Takashi,Koyanagi Yoshio
- PLOS ONE 10(3), 2015-03-17
- … DNA-editing technology has made it possible to rewrite genetic information in living cells. … Human immunodeficiency virus (HIV) provirus, an integrated form of viral complementary DNA in host chromosomes, could be a potential target for this technology. …
- NAID 120005619759
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★リンクテーブル★
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- 英
- target DNA
- 関
- 標的DNA
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[★]
- 英
- deoxyribonucleic acid
- 同
- デオキシリボ核酸
- 関
- リボ核酸 RNA
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ターゲティング、標的指向化