関: PCR、polymerase chain reaction

WordNet

inhabit a nest, usually after building; "birds are nesting outside my window every Spring"
a kind of gun emplacement; "a machine-gun nest"; "a nest of snipers"
furniture pieces made to fit close together
a cosy or secluded retreat
a gang of people (criminals or spies or terrorists) assembled in one locality; "a nest of thieves"
a structure in which animals lay eggs or give birth to their young
fit together or fit inside; "nested bowls"
gather nests
the 16th letter of the Roman alphabet (同)p

PrepTutorEJDIC

(鳥・昆虫・魚類・小動物などの)『巣』,巣穴 / 《集合的に》巣の中の鳥(動物など),(昆虫などの)群れ / 居ごこちよい場所,落ちつける場所 / (犯罪などの)温床;(悪党などの)巣窟(そうくつ) / (入れ子式・重箱式などになった物の)一組,一そろい《+『of』+『名』》 / 巣を作る,巣ごもる / …‘を'巣に入れる / (巣に入れるように)…‘を'すっぽり収める / …‘を'入れ子式にする,重ね合わせる
Peace Corps 平和部隊
parking
phosphorusの化学記号

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/07/17 18:28:45」(JST)

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A diagram illustrating the method of nested PCR.

Nested polymerase chain reaction is a modification of polymerase chain reaction intended to reduce non-specific binding in products due to the amplification of unexpected primer binding sites.

Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature-mediated DNA polymerase. The products can be used for sequencing or analysis, and this process is a key part of many genetics research laboratories, along with uses in DNA fingerprinting for forensics and other human genetic cases. Conventional PCR requires primers complementary to the termini of the target DNA. A commonly occurring problem is primers binding to incorrect regions of the DNA, giving unexpected products.

Nested polymerase chain reaction involves two sets of primers, used in two successive runs of polymerase chain reaction, the second set intended to amplify a secondary target within the first run product.

Processes[edit]

The target DNA undergoes the first run of polymerase chain reaction with the first set of primers, shown in green. The selection of alternative and similar primer binding sites gives a selection of products, only one containing the intended sequence.
The product from the first reaction undergoes a second run with the second set of primers, shown in red. It is very unlikely that any of the unwanted PCR products contain binding sites for both the new primers, ensuring the product from the second PCR has little contamination from unwanted products of primer dimers, hairpins, and alternative primer target sequences.

Resources[edit]

Books at your local library about nested polymerase chain reacitons
Scholarly articles on nested polymerase chain reactions

UpToDate Contents

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1. ポリメラーゼ連鎖反応(PCR) polymerase chain reaction
2. ハンタウイルス感染の疫学および診断 epidemiology and diagnosis of hantavirus infections
3. Q熱の臨床症状および診断 clinical manifestations and diagnosis of q fever
4. ツツガ虫病：臨床的特徴および診断 scrub typhus clinical features and diagnosis
5. 梅毒の診断的検査 diagnostic testing for syphilis

English Journal

Circulating and disseminated tumor cells in the management of advanced prostate cancer.

Kruck S, Gakis G, Stenzl A.SourceDepartment of Urology, Eberhard-Karls University, Hoppe Seyler-Strase 3, 72076 TUbingen, Germany.
Advances in urology.Adv Urol.2012;2012:135281. Epub 2011 Aug 21.
Management of prostate cancer is recognized as one of the most important medical problems. Latest findings concerning the role of circulating (CTC) and disseminated tumor cells (DTC) have provided new insights into the biology of metastasis with important implications for the clinical management of
PMID 21869883

Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats.

Yan S, Zheng C, Chen ZQ, Liu R, Li GG, Hu WK, Pei H, Li B.SourceDepartment of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jie-fang Road, Wuhan, Hubei Province 430030, China.
Experimental diabetes research.Exp Diabetes Res.2012;2012:743780. Epub 2011 Aug 28.
Recent reports show that ER stress plays an important role in diabetic retinopathy (DR), but ER stress is a complicated process involving a network of signaling pathways and hundreds of factors, What factors involved in DR are not yet understood. We selected 89 ER stress factors from more than 200,
PMID 21904541

Japanese Journal

周産期領域におけるG 型肝炎ウイルスの臨床的意義─同じフラビウイルス科に属するC 型肝炎ウイルスと比較して─

西川正能,大島教子,林田綾子,林田志峯,石川和明,岡嶋祐子,北澤正文,深澤一雄,渡辺博,高見澤裕吉,稻葉憲之
Dokkyo journal of medical sciences 38(1), 49-57, 2011-03-25
… 当科を受診した妊婦3,738 名(HGV),4,023 名(HCV)とキャリア妊婦の出生児14 名(HGV),24 名(HCV)である.HGV RNA は RT-PCR 法(定性)k,real-time PCR(定量)及びcycle-sequence 法(genome sequence)により,HGV-E2 抗体はELISA 法を用いて,またHCV RNA はnested RT-PCR(定性),real- time PCR(定量)により,またHCV-Ab は2nd 及び3rd generation EIA を用いて測定した.対象児の血清サンプルは臍帯血から …
NAID 110008427652

Genetically Diverse Group C Rotaviruses Cause Sporadic Infection in Korean Calves

PARK Sang-Ik,JEONG Young-Ju,KIM Hyun-Jeong,PARK Jun-Gyu,KANG Shien-Young,WOO Sang-Kyu,KIM Chae-Hyeon,JUNG Cheong-Hwan,KANG Mun-Il,CHO Kyoung-Oh
Journal of Veterinary Medical Science 73(4), 479-482, 2011
… This study examined the prevalence and genetic diversity of the bovine group C rotaviruses (GCRVs) in a total of 127 diarrhea fecal samples of calves from 52 Korean native beef calf herds using RT-PCR and nested PCR. … Overall, seven of the 127 fecal samples (5.5%) from seven of the 52 herds (13.5%) tested positive for bovine GCRVs only by nested PCR. …
NAID 130000444461