関: FBS、FCS、fetal bovine serum

WordNet

fine leather from the skin of a calf (同)calfskin
young of domestic cattle
young of various large placental mammals e.g. whale or giraffe or elephant or buffalo
the muscular back part of the shank (同)sura
of or relating to a fetus; "fetal development" (同)foetal
an amber, watery fluid, rich in proteins, that separates out when blood coagulates (同)blood_serum

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{C}『子牛』 / 〈C〉(象・鯨・アザラシ・鹿などの)幼獣 / 〈U〉=calfskin
ふくらはぎ
リンパ液 / 血清
serial / series / sermon
circa

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/11/01 18:34:10」(JST)

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Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells.^[1] Fetal bovine serum comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications.

The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum. The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and divide.

Production

Fetal bovine serum is a by-product of the Dairy Industry. Fetal bovine serum, as with the vast majority of animal serum used in cell culture, is produced from blood collected at commercial slaughterhouses from dairy cattle that also supply meat destined for human consumption.

The first stage of the production process for fetal bovine serum is the harvesting of blood from the bovine fetus after the fetus is removed from the slaughtered cow. The blood is collected aseptically into a sterile container or blood bag and then allowed to clot. The normal method of collection is cardiac puncture, wherein a needle is inserted into the heart. This minimizes "the danger of serum contamination with micro-organisms from the fetus itself, and the environment".^[1] It is then centrifuged to remove the fibrin clot and the remaining blood cells from the clear yellow (straw) colored serum. The serum is frozen prior to further processing that is necessary to make it suitable for cell culture.

The second stage of processing involves filtration, typically using a filtration chain with the final filtration being triple sterile 0.1 micrometre membrane filters. When processed by a reputable commercial serum supplier, the sterilized fetal bovine serum is subjected to stringent quality control testing and is supplied with a detailed Certificate of Analysis. The certificate gives full test results and information concerning the origin of the serum. Certificates of Analysis vary between commercial suppliers, but each usually includes the following details: filtration statement, country of origin, cell growth performance testing, microbial sterility testing, screening for mycoplasma and virus, endotoxin, haemoglobin, IgG and total protein assays.

Ethics

Ethical questions have been raised regarding the blood collection process due to the potential suffering caused to the fetus. Research^[1]^[2] is conflicting over whether fetal anoxia is likely to cause death prior to serum harvesting and whether bovine embryos are capable of experiencing pain. Ethical concerns surrounding the welfare of the donor animal are weighed against fetal bovine serum's beneficial use in human and animal health research.

Serum use

Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their specific cell type. When changing from batch to batch it is usual to adapt the cells to the new batch of material, for example, by mixing 50% of the old serum with 50% of the new serum and allowing the cells to acclimatise to the new material.

Serum is stored frozen to preserve the stability of components such as growth factors. When serum is thawed, some precipitation may be seen. This is a normal phenomenon and it does not compromise the quality of serum in any way. The precipitate may be removed by transferring the serum to sterile tubes and centrifuging for 5 minutes at 400 x g.

To limit the amount of precipitation, it is recommended that the serum is thawed in a refrigerator at 2-8˚C. The serum should be regularly mixed during this process. Repeated freeze/thaw cycles should be avoided and it is advisable to dispense the serum into single use aliquots before freezing.

Source history

Serum produced for use in the biotechnology industry and research sectors is highly regulated. The collection and movement of all animal derived products globally is strictly controlled. Veterinary control of animal derived products largely follows the regulations set by the EU (DG SANCO) and the US (USDA). The current regulation governing the importation of animal by-products into the EU is covered by Regulation (EU) 1069/2009 and the implementation document Regulation (EU) 142/2011.

FBS is a product collected worldwide with the main collections being centered in the United States, Australia, New Zealand, Canada, Central America, South America and Europe.

Global sales

Sales of FBS in 2008 were estimated to be 700,000 litres globally.

Major companies that collect and sell FBS globally are members of the ISIA, the International Serum Industry Association. Member companies are listed on the ISIA website. Full contact details are available through this portal and the ISIA is able to answer any questions concerning the collection and use of this important resource.

The International Serum Industry Association was incorporated in June 2006 to self-regulate a previously unstandardized industry. The ISIA Mission Statement states: “ISIA shall establish, promote and assure compliance with uncompromised standards of excellence and ethics in the business practices of the global animal serum and animal derived products supply industry. Our primary focus will be on safety and safe use of serum and animal derived products through proper origin traceability, truth in labeling and appropriate standardization and oversight.”^[3]

References

^ ^a ^b ^c Jochems, Carlo; et al. (April–March 2002). "The Use of Fetal Bovine Serum: Ethical or Scientific Problem?". Fund for the Replacement of Animals in Medical Experiments 30 (2): 219–227. Retrieved 24 April 2012. Check date values in: |date= (help)
^ Mellor DJ, Gregory NG (2003). "Responsiveness, behavioural arousal and awareness in fetal and newborn lambs: experimental, practical and therapeutic implications.". N Z Vet J 51 (1): 2–13. doi:10.1080/00480169.2003.36323. PMID 16032283.
^ "Serum Industry". Retrieved 22 February 2013.

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English Journal

A clinically-feasible protocol for using human platelet lysate and mesenchymal stem cells in regenerative therapies.

Warnke PH, Humpe A, Strunk D, Stephens S, Warnke F, Wiltfang J, Schallmoser K, Alamein M, Bourke R, Heiner P, Liu Q.SourceClem Jones Research Centre For Stem Cells & Tissue Regenerative Therapies, Bond University, Gold Coast, QLD, Australia; Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany. Electronic address: pwarnke@bond.edu.au.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery.J Craniomaxillofac Surg.2013 Mar;41(2):153-61. doi: 10.1016/j.jcms.2012.07.003. Epub 2012 Aug 9.
The transplantation of human stem cells seeded on biomaterials holds promise for many clinical applications in cranio-maxillo-facial tissue engineering and regenerative medicine. However, stem cell propagation necessary to produce sufficient cell numbers currently utilizes fetal calf serum (FCS) as
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Coumarin-DPA-Cu(ii) as a chemosensing ensemble towards histidine determination in urine and serum.

Hou JT, Li K, Yu KK, Wu MY, Yu XQ.SourceKey Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China. kli@scu.edu.cn xqyu@scu.edu.cn.
Organic & biomolecular chemistry.Org Biomol Chem.2013 Feb 7;11(5):717-20. doi: 10.1039/c2ob26955d. Epub 2012 Dec 13.
An ensemble-based fluorescent sensor HS-1 + Cu(2+) for detection of histidine is reported. Complex HS-1 + Cu(2+) sensitively senses histidine at pH 7.4 in aqueous media. The quantitative determination of histidine in urine and fetal calf serum is also conducted.
PMID 23238391

Growth factor regulation of intracellular pH homeostasis under hypoxic conditions in isolated equine articular chondrocytes.

Milner PI, Smith HC, Robinson R, Wilkins RJ, Gibson JS.SourceFaculty of Health and Life Sciences, Department of Musculoskeletal Biology, University of Liverpool, Leahurst Campus, Neston CH64 7TE, United Kingdom. p.i.milner@liverpool.ac.uk.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society.J Orthop Res.2013 Feb;31(2):197-203. doi: 10.1002/jor.22221. Epub 2012 Sep 14.
Hypoxia and acidosis are recognized features of inflammatory arthroses. This study describes the effects of IGF-1 and TGF-β(1) on pH regulatory mechanisms in articular cartilage under hypoxic conditions. Acid efflux, reactive oxygen species (ROS), and mitochondrial membrane potential were measured
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Japanese Journal

Survival of Enveloped and Non-Enveloped Viruses on Inanimate Surfaces

Microbes and Environments 30(2), 140-144, 2015
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Survival of Enveloped and Non-Enveloped Viruses on Inanimate Surfaces

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Influence of Valine Analogues on Protein Synthesis of Chicken Embryo Myoblasts

The Journal of Poultry Science 51(2), 191-194, 2014
NAID 130004725699

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★リンクテーブル★

リンク元	「FBS」「ウシ胎児血清」「fetal bovine serum」「FCS」「ウシ胎仔血清」
関連記事	「fetal」「calf」「calf serum」「fetal calf」「cal」