WordNet
- (microscopy) a dye or other coloring material that is used in microscopy to make structures visible
- color for microscopic study; "The laboratory worker dyed the specimen"
- color with a liquid dye or tint; "Stain this table a beautiful walnut color"; "people knew how to stain glass a beautiful blue in the middle ages"
- produce or leave stains; "Red wine stains the table cloth"
- a soiled or discolored appearance; "the wine left a dark stain" (同)discoloration, discolouration
- marked or dyed or discolored with foreign matter; "a badly stained tablecloth"; "tear-stained cheeks"
- having a coating of stain or varnish (同)varnished
- the act of spotting or staining something (同)spotting, maculation
- (histology) the use of a dye to color specimens for microscopic study
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- (…で)…‘を'『汚す』,‘に'しみをつける《+『名』+『with』+『名』》 / 〈ガラス・木材・布など〉‘に'『着色する』 / (…で)〈人格・名声など〉‘を'汚す,‘に'傷をつける《+『名』+『with』+『名』》 / 汚れる,しみがつく / 〈C〉〈U〉(…についた)『しみ』,汚れ《+『on』+『名』》 / 〈U〉〈C〉着色剤,染料 / 〈C〉(人格・名声などに対する)汚点,傷《+『on』(『upon』)+『名』》
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/10/03 22:15:55」(JST)
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Giemsa stained
Trypanosoma parasites (Chagas disease pathogen)
Whirling disease section stained with Giemsa
Giemsa stain, named after Gustav Giemsa, an early German microbiologist, is used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.[1]
Contents
- 1 Uses
- 2 Generation
- 3 See also
- 4 References
Uses[edit]
It is specific for the phosphate groups of DNA and attaches itself to regions of DNA where there are high amounts of adenine-thymine bonding. Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create an idiogram. It can identify chromosomal aberrations such as translocations and rearrangements.
Giemsa stain is also a differential stain. It can be used to study the adherence of pathogenic bacteria to human cells. It differentially stains human and bacterial cells purple and pink respectively. It can be used for histopathological diagnosis of malaria[2] and some other spirochete and protozoan blood parasites. It is also used in Wolbach's tissue stain.
Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens. Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta.
Giemsa stain is also used to visualize chromosomes.
Giemsa stains the fungus histoplasma, chlamydia bacteria, and can be used to identify Mast cells.[3]
Generation[edit]
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder.
A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.[4]
See also[edit]
- Biological stains and staining protocols
- Histology
- Microscopy
- Romanowsky stain
- Wright's stain
References[edit]
- ^ Giemsa G (1904 Eine Vereinfachung und Vervollkommnung meiner Methylenblau-Eosin-Färbemethode zur Erzielung der Romanowsky-Nocht’schen Chromatinfärbung. Centralblatt für Bakteriologie I Abteilung 32, 307–313.
- ^ Shapiro HM, Mandy F (September 2007). "Cytometry in malaria: moving beyond Giemsa". Cytometry. Part A : the journal of the International Society for Analytical Cytology 71 (9): 643–5. doi:10.1002/cyto.a.20453. PMID 17712779.
- ^ Damsgaard TE, Olesen AB, Sørensen FB, Thestrup-Pedersen K, Schiøtz PO (April 1997). "Mast cells and atopic dermatitis. Stereological quantification of mast cells in atopic dermatitis and normal human skin". Arch. Dermatol. Res. 289 (5): 256–60. doi:10.1007/s004030050189. PMID 9164634.
- ^ "4.2.2.2".
Stains
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Iron/Hemosiderin |
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Lipids |
- Sudan stain
- Sudan II
- Sudan III
- Sudan IV
- Oil Red O
- Sudan Black B
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Carbohydrates |
- Periodic acid-Schiff stain
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Amyloid |
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Bacteria |
- Gram staining
- Methyl violet/Gentian violet
- Safranin
- Ziehl–Neelsen stain/acid-fast
- Carbol fuchsin/Fuchsine
- Methylene blue
- Auramine-rhodamine stain
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Connective tissue |
- trichrome stain: Masson's trichrome stain/Lillie's trichrome
- Light Green SF yellowish
- Biebrich scarlet
- Phosphomolybdic acid
- Fast Green FCF
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Other |
- H&E stain
- Silver stain
- Grocott's methenamine silver stain
- Warthin–Starry stain
- Methyl blue
- Wright's stain
- Giemsa stain
- Gömöri trichrome stain
- Neutral red
- Janus Green B
- Alcian blue stain
- Movat's stain
|
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Tissue stainability |
- Acidophilic
- Basophilic
- Chromophobic
|
|
UpToDate Contents
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English Journal
- Prospective randomized controlled study comparing cell block method and conventional smear method for pancreatic juice cytology.
- Noda Y, Fujita N, Kobayashi G, Ito K, Horaguchi J, Obana T, Koshita S, Kanno Y, Yamashita Y, Kato Y, Ogawa T, Tsuchiya T, Oikawa M, Sawai T, Kanno H, Kurose A.SourceDepartments of Gastroenterology Surgery, Sendai City Medical Center, Sendai First Department of Pathology, Iwate Medical University, Morioka, Japan.
- Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society.Dig Endosc.2012 May;24(3):168-74. doi: 10.1111/j.1443-1661.2011.01180.x. Epub 2011 Jul 13.
- Aim: To elucidate the diagnostic efficacy of the cell block (CB) method by comparing it with that of conventional smear cytology for pancreatic juice obtained by endoscopic retrograde cholangiopancreatography (ERCP) in a randomized controlled trial fashion. Methods: A total of 170 patients wit
- PMID 22507091
Japanese Journal
- Rapid-Air-Dry Papanicolaou Stain in Canine and Feline Tumor Cytology: A Quantitative Comparison with the Giemsa Stain
- SAWA Mariko,YABUKI Akira,MIYOSHI Noriaki [他],ARAI Kou,YAMATO Osamu
- Journal of Veterinary Medical Science 74(9), 1133-1138, 2012
- … The Papanicolaou stain is a gold-standard staining method for tumor diagnosis in human cytology. … Currently, a rapid Papanicolaou stain using air-dried smears is utilized in human cytology, but usefulness of this rapid-air-dry Papanicolaou (RAD-Pap) stain in the veterinary field has not been fully evaluated. … The purpose of this study was to evaluate the usefulness of the RAD-Pap stain by using quantitative analysis. …
- NAID 130001879750
- Fluorescence in situ hybridization(FISH)の末梢血塗抹標本への応用
- 生田 幹博,大神 明子,高松 泰 [他],阿南 建一,三浦 偉久男,加藤 純子,石田 寛美,二田 優子,川島 博信,松永 彰,Ikuta Mikihiro,Ogami Akiko,Takamatsu Yasushi,Anami Ken'ichi,Miura Ikuo,Kato Junko,Ishida Hiromi,Futata Yuko,Kawashima Hironobu,Matsunaga Akira,イクタ ミキヒロ,オガミ アキコ,タカマツ ヤスシ,アナミ ケンイチ,ミウラ イクオ,カトウ ジュンコ,イシダ ヒロミ,フタタ ユウコ,カワシマ ヒロノブ,マツナガ アキラ
- 福岡医学雑誌 102(11), 318-324, 2011-11-25
- … Whether peripheral-blood preparations after May-Grunwald Giemsa staining could be used for fluorescence in situ hybridization (FISH). …
- NAID 40019177299
Related Links
- 4 Garcia (Giemsa Stain) The morphology of these changes in the life cycle and within the RBCs can cause confusion when examining blood films prepared from this blood. a. Stippling (Schüffner’s dots) may not be visible. b. The ...
- ( http://www.abnova.com ) - Wright and Giemsa stains are used to stain peripheral blood and bone marrow smears. The components are oxidized eosin Y, methylen... ... Uploaded on Dec 30, 2010 ( http://www.abnova ...
Related Pictures
★リンクテーブル★
[★]
- 英
- Giemsa stain, Giemsa staining
- 同
- Giemsa染色、ギームザ染色
- 関
- Giemsa液、ギムザ単染色 Giemsa単染色
- 染色液はアズールIIとアズールII・エオジンからなる
- アズールII:塩基性色素:青紫色:核のDNA、細胞質のDNA、アズール顆粒
- エオジン:酸性色素:赤橙色:ヘモグロビン、好酸性顆粒
微生物の染色
- グラム染色が困難なのでギムザ染色を長時間行う
[★]
アズール色素
- 関
- Giemsa stain
[★]
メイ・グリュンワルト・ギムザ染色
[★]
- 関
- coloring agent、dye、staining、tissue stain