全反射照明蛍光顕微鏡、全反射照明蛍光顕微鏡観察、全反射照明蛍光顕微鏡法、全反射照明蛍光顕微法
- 関
- TIRFM
WordNet
- the phenomenon of a propagating wave (light or sound) being thrown back from a surface (同)reflexion
- (mathematics) a transformation in which the direction of one axis is reversed
- the image of something as reflected by a mirror (or other reflective material); "he studied his reflection in the mirror" (同)reflexion
- the ability to reflect beams or rays (同)reflexion, reflectivity
- determine the sum of; "Add all the people in this town to those of the neighboring town" (同)tot, tot up, sum, sum_up, summate, tote up, add, add together, tally, add up
- damage beyond the point of repair; "My son totaled our new car"; "the rock star totals his guitar at every concert"
- add up in number or quantity; "The bills amounted to $2,000"; "The bill came to $2,000" (同)number, add up, come, amount
- work as an intern; "The young doctor is interning at the Medical Center this year"
- an advanced student or graduate in medicine gaining supervised practical experience (`houseman is a British term) (同)interne, houseman, medical intern
- deprive of freedom; "During WW II, Japanese were interned in camps in the West"
- happening or arising or located within some limits or especially surface; "internal organs"; "internal mechanism of a toy"; "internal party maneuvering"
- occurring within an institution or community; "intragroup squabbling within the corporation" (同)intragroup
- research with the use of microscopes
PrepTutorEJDIC
- 〈U〉(光・熱などの)『反射』,(音の)反響;反射光(熱),反響音《+『of』+『名』》 / 〈C〉(鏡・水面などに映った)『映像』;(親などの)生き写し《+『of』+『名』》 / 〈C〉(気持ち・性格などの)表れ,反映《+『of』+『名』》 / 〈U〉(…についての)『熟考』,沈思《+『on』(『upon』)+『名』》 / 〈C〉《しばしば複数形で》(熟考して得られた)(…についての)意見,考え《+『on』(『upon』)+『名』》 / 〈C〉(…に対する)非難,小言;(…にとって)不名誉となるもの《+『on』(『upon』)+『名』》
- 『総計の』,全体の / 『全くの』,完全な(absolute) / 『合計』,総額,総数,総量(sum) / …‘を'合計する / 合計…‘に'なる,総額が…‘に'のぼる(amount to)
- (特に戦時中)〈人・船など〉‘を'強制収容する,抑留する
- 『内側の』,内部にある / 『国内の』(domestic) / 内からとる,内用の
- 顕微鏡使用[法] / 顕微鏡による検査
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/04/05 17:17:36」(JST)
[Wiki en表示]
(Cis-)total internal reflection fluorescence microscope (TIRFM) diagram
- Specimen
- Evanescent wave range
- Cover slip
- Immersion oil
- Objective
- Emission beam (signal)
- Excitation beam
(Trans-)total internal reflection fluorescence microscope (TIRFM) diagram
- Objective
- Emission beam (signal)
- Immersion oil
- Cover slip
- Specimen
- Evanescent wave range
- Excitation beam
- Quartz prism
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nm can be observed.
Contents
- 1 Background
- 2 Overview
- 3 References
- 4 External links
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Background
In cell and molecular biology, a large number of molecular events in cellular surfaces such as cell adhesion, binding of cells by hormones, secretion of neurotransmitters, and membrane dynamics have been studied with conventional fluorescence microscopes. However, fluorophores that are bound to the specimen surface and those in the surrounding medium exist in an equilibrium state. When these molecules are excited and detected with a conventional fluorescence microscope, the resulting fluorescence from those fluorophores bound to the surface is often overwhelmed by the background fluorescence due to the much larger population of non-bound molecules.
Overview
The idea of using total internal reflection to illuminate cells contacting the surface of glass was first described by Ambrose in 1956.[1] This idea was then extended by Daniel Axelrod[2] at the University of Michigan, Ann Arbor in the early 1980s as TIRFM. A TIRFM uses an evanescent wave to selectively illuminate and excite fluorophores in a restricted region of the specimen immediately adjacent to the glass-water interface. The evanescent wave is generated only when the incident light is totally internally reflected at the glass-water interface. The evanescent electromagnetic field decays exponentially from the interface, and thus penetrates to a depth of only approximately 100 nm into the sample medium. Thus the TIRFM enables a selective visualization of surface regions such as the basal plasma membrane (which are about 7.5 nm thick) of cells as shown in the figure above. Note, however, that the region visualised is at least a few hundred nanometers wide, so the cytoplasmic zone immediately beneath the plasma membrane is necessarily visualised in addition to the plasma membrane during TIRF microscopy. The selective visualisation of the plasma membrane renders the features and events on the plasma membrane in living cells with high axial resolution.
TIRF can also be used to observe the fluorescence of a single molecule,[3][4] making it an important tool of biophysics and quantitative biology.
References
- ^ Ambrose, EJ (24). "A surface contact microscope for the study of cell movements.". Nature. 178(4543) (4543): 1194. Bibcode:1956Natur.178.1194A. doi:10.1038/1781194a0.
- ^ Axelrod, D. (1 April 1981). "Cell-substrate contacts illuminated by total internal reflection fluorescence". The Journal of Cell Biology 89 (1): 141–145. doi:10.1083/jcb.89.1.141. PMC 2111781. PMID 7014571. Retrieved 16 January 2012.
- ^ Yanagida, Toshio; Sako, Yasushi, Minoghchi, Shigeru (10 February 2000). "Single-molecule imaging of EGFR signalling on the surface of living cells". Nature Cell Biology 2 (3): 168–172. doi:10.1038/35004044.
- ^ Andre et al. Cross-correlated tirf/afm reveals asymmetric distribution of forcegenerating heads along self-assembled, synthetic myosin filaments. Biophysical Journal, 96:1952–1960, 2009.
- Axelrod, Daniel (1 November 2001). "Total Internal Reflection Fluorescence Microscopy in Cell Biology". Traffic 2 (11): 764–774. doi:10.1034/j.1600-0854.2001.21104.x.
External links
- Interactive Fluorescence Dye and Filter Database Carl Zeiss Interactive Fluorescence Dye and Filter Database.
- cell^TIRF - Multicolor TIRF - Olympus commercial TIRF microscope systems
- Leica Microsystems commercial TIRF microscope systems
- TIRF Microscopy: Introduction and Applications TIRF Tutorial from Microscopy U
- TIRF Microscopy: Overview TIRF Tutorial from Olympus Microscopy Resource Center
- Olympus TIRFM Microscopes commercial TIRF microscope systems
- Carl Zeiss Laser TIRF 3 commercial TIRF microscope systems
- Lightguide- and prism-based TIRF microscopy Commercial TIRF Microscopy, TIRF Spectroscopy, TIRF ElectroChemistry, and TIRF Dielectrophoresis systems
- TIRF FLIM microscopy Lambert Instruments TIRF - FLIM microscopy
- TIRF Microscopy:Leica Science Lab
Optical microscopy
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- Microscope
- Optical microscopy
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Illumination and
contrast methods |
- Bright field microscopy
- Köhler illumination
- Dark field microscopy
- Phase contrast
- Differential interference contrast (DIC)
- Dispersion staining
- Second harmonic imaging (SHIM)
- 4Pi microscope
- Structured illumination
- Sarfus
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Fluorescence methods |
- Fluorescence microscopy
- Confocal microscopy
- Two-photon excitation microscopy
- Multiphoton microscopy
- Image deconvolution
- Total internal reflection fluorescence microscopy (TIRF)
- Lightsheet microscopy (LSFM/SPIM)
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Sub-diffraction
limit techniques |
- Diffraction limit
- Stimulated emission depletion (STED)
- Photo-activated localization microscopy (PALM)
- Near-field (NSOM/SNOM)
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UpToDate Contents
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English Journal
- The Na+ /H+ -exchanger (NHE1) generates pH nanodomains at focal adhesions.
- Ludwig FT, Schwab A, Stock C.SourceInstitute of Physiology II, University of Münster, Robert-Koch-Str. 27b, Münster, Germany. f_ludw01@uni-muenster.de
- Journal of cellular physiology.J Cell Physiol.2013 Jun;228(6):1351-8. doi: 10.1002/jcp.24293.
- Many tumor cells are characterized by an increased net acid production. They extrude the excess protons mainly through the Na(+) /H(+) -exchanger NHE1. An increased NHE1 activity elevates the metastatic potential of tumor cells. Cell migration, a key step in the metastatic cascade, requires the form
- PMID 23184727
- An initial and rapid step of lytic granule secretion precedes microtubule organizing center polarization at the cytotoxic T lymphocyte/target cell synapse.
- Bertrand F, Müller S, Roh KH, Laurent C, Dupré L, Valitutti S.SourceSection Dynamique Moléculaire des Interactions Lymphocytaires, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 1043, Centre de Physiopathologie de Toulouse Purpan, 31024 Toulouse, France.
- Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2013 Apr 9;110(15):6073-8. doi: 10.1073/pnas.1218640110. Epub 2013 Mar 27.
- It is presently assumed that lethal hit delivery by cytotoxic T lymphocytes (CTLs) is mechanistically linked to centrosome polarization toward target cells, leading to dedicated release of lytic granules within a confined secretory domain. Here we provide three lines of evidence showing that this me
- PMID 23536289
- Drosophila homologues of Adenomatous polyposis coli (APC) and the formin Diaphanous collaborate by a conserved mechanism to stimulate actin filament assembly.
- Jaiswal R, Stepanik V, Rankova A, Molinar O, Goode BL, McCartney B.SourceBrandeis University, United States;
- The Journal of biological chemistry.J Biol Chem.2013 Apr 4. [Epub ahead of print]
- Adenomatous polyposis coli (APC) is a large multi-domain protein that regulates the cytoskeleton. Recently, it was shown that vertebrate APC through its Basic domain directly collaborates with the formin mDia1 to stimulate actin filament assembly in the presence of nucleation barriers. However, it h
- PMID 23558679
Japanese Journal
- Optical Observation of Deep Bulk Damage in Amorphous Perfluorocarbon Films Produced by UV Photons Emitted from Low-Pressure Argon Plasma
- Ono Takao,Iizuka Ryo,Akagi Takanori [他]
- Journal of photopolymer science and technology 27(3), 393-398, 2014
- NAID 40020133215
- 1J2-1 全反射蛍光顕微鏡とフローインジェクション型無線無電極QCMシステムの融合(測定技術,映像法,非破壊検査)
- 福島 政比古,上杉 研太郎,荻 博次,八木 寿梓,後藤 裕児,平尾 雅彦
- 超音波エレクトロニクスの基礎と応用に関するシンポジウム講演論文集 (33), 1-2, 2012-11-13
- NAID 110009619702
- Novel Situations of Endothelial Injury in Stroke-Mechanisms of Stroke and Strategy of Drug Development : Novel Mechanism of the Expression and Amplification of Cell Surface-Associated Fibrinolytic Activity Demonstrated by Real-Time Imaging Analysis
- Suzuki Yuko,Urano Tetsumei
- Journal of pharmacological sciences 116(1), 19-24, 2011-05-20
- … We have recently demonstrated its unique secretory dynamics in GFP-tagged tPA expressing VECs using total internal reflection fluorescence microscopy. …
- NAID 10029893747
Related Pictures
★リンクテーブル★
[★]
- 英
- total internal reflection fluorescence microscopy
- (方法)total internal reflection fluorescence microscopy、TIRFM
- 関
- 全反射照明蛍光顕微鏡法、全反射照明蛍光顕微鏡観察、全反射照明蛍光顕微法
[★]
- 英
- total internal reflection fluorescence microscopy TIRFM
- 関
- 全反射照明蛍光顕微鏡法、全反射照明蛍光顕微鏡観察、全反射照明蛍光顕微鏡
[★]
- 英
- total internal reflection fluorescence microscopy
- 関
- 全反射照明蛍光顕微鏡観察、全反射照明蛍光顕微法、全反射照明蛍光顕微鏡
[★]
- 英
- total internal reflection fluorescence microscopy
- 関
- 全反射照明蛍光顕微鏡法、全反射照明蛍光顕微法、全反射照明蛍光顕微鏡
[★]
- 関
- total internal reflection fluorescence microscopy
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- 関
- light microscopy、microscope、microscopic、microscopic examination、microscopic test、microscopical
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- 関
- all、altogether、common、entire、entirety、gross、overall、total amount、whole
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蛍光顕微鏡、蛍光顕微鏡観察、蛍光顕微鏡法、蛍光顕微法
- 関
- fluorescence microscope、fluoroscopically、immunofluorescence microscopy
[★]
- 関
- endo、in vivo、interior、internally、intra
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- reflect、reflective、reflex