phosphate-buffered saline

リン酸緩衝食塩水

関

PBS

WordNet

1. add a buffer (a solution); "buffered saline solution for the eyes"
2. (chemistry) an ionic compound that resists changes in its pH
3. (computer science) a part of RAM used for temporary storage of data that is waiting to be sent to a device; used to compensate for differences in the rate of flow of data between components of a computer system (同)buffer storage, buffer store
4. a cushion-like device that reduces shock due to an impact (同)fender
5. a power tool used to buff surfaces (同)polisher
6. a salt of phosphoric acid (同)orthophosphate, inorganic_phosphate
7. carbonated drink with fruit syrup and a little phosphoric acid
8. containing salt; "a saline substance "; "salty tears"

PrepTutorEJDIC

1. (鉄道車両などの衝突の衝撃を弱める)緩衝器 / 間に立って争いや衝突の働撃を弱める人(物) / 物をみがく人(道具) / …'を'緩和する,和らげる / …'を'緩衝液で処理する
2. ばかな老人 / やつ
3. 〈U〉リン酸塩 / 《複数形で》リン酸肥料
4. 塩の,塩分のある / (薬用の)塩類,含塩物;〈C〉含塩下剤

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/12/28 18:25:42」(JST)

wiki en

[Wiki en表示]

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing sodium dihydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium hydrogen phosphate. The osmolarity and ion concentrations of the solutions match those of the human body (isotonic).

Applications

PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing. PBS with EDTA is also used to disengage attached and clumped cells. Divalent metals such as zinc, however, cannot be added as this will result in precipitation. For these types of applications, Good's buffers are recommended.

Preparation

There are many different ways to prepare PBS. Some formulations do not contain potassium, while others contain calcium or magnesium.

The most common composition of PBS (1X)

Salt	Concentration (mmol/L)	Concentration (g/L)
NaCl	137	8.0
KCl	2.7	0.2
Na2HPO4	10	1.42
KH2PO4	1.8	0.24

Start with 800 mL of distilled water to dissolve all salts. Adjust the pH to 7.4 with HCl. Add distilled water to a total volume of 1 liter. The resultant 1x PBS should have a final concentration of 10 mM PO₄³⁻, 137 mM NaCl, and 2.7 mM KCl.

Cold Spring Harbor Protocol

reagent	MW	mass (g) 10X	[M] 10X	mass (g) 5X	[M] 5X	mass (g) 1X	[M] 1X
Na2HPO4	141.95897	14.1960	0.1000	7.0980	0.0500	1.41960	0.0100
KH2PO4	136.08569	2.4496	0.0180	1.2248	0.0090	0.24496	0.0018
NaCl	58.44300	80.0669	1.3700	40.0335	0.6850	8.00669	0.1370
KCl	74.55150	2.0129	0.0270	1.0064	0.0135	0.20129	0.0027
pH = 7.4

The simplest way to prepare a PBS solution is to use PBS buffer tablets or pouches. They are formulated to give a ready-to-use PBS solution upon dissolution in a specified quantity of distilled water. They are available in the standard volumes: 100, 200, 500 and 1000 mL, and 10, 25, 50 and 100 L.

If used in cell culturing, the solution can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle). Sterilization may not be necessary depending on its use. PBS can be stored at room temperature or in the fridge. However, concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.

See also

• Borate-buffered saline
• Tris-buffered saline

References

Cold Spring Harbor Protocols

UpToDate Contents

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English Journal

• A direct competitive enzyme-linked immunosorbent assay for rapid detection of anilofos residues in agricultural products and environmental samples.

• Zhang Y, Gao AH, Liu B, Sheng W, Tan C, Yuan M, Wang S.Sourcea Key Laboratory of Food Nutrition and Safety, Ministry of Education of China , Tianjin University of Science and Technology , Tianjin , China.
• Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.J Environ Sci Health B.2013;48(1):1-8. doi: 10.1080/03601234.2012.716667.
• A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed to measure anilofos levels in agricultural and environmental samples. The ELISA was developed using rabbit polyclonal antibodies against a hapten-protein conjugate of anilofos-bovine serum albumin. The limit of detection
• PMID 23030434

• Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples.

• Zhou Y, Tian XL, Li YS, Pan FG, Zhang YY, Zhang JH, Wang XR, Ren HL, Lu SY, Li ZH, Liu ZS, Chen QJ, Liu JQ.SourceKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China. zhouyu69@sina.com
• Food chemistry.Food Chem.2012 Dec 15;135(4):2661-5. doi: 10.1016/j.foodchem.2012.07.047. Epub 2012 Jul 15.
• Abrin is a plant toxin, which can be easily isolated from the seeds of Abrus precatorius. It may be used as a biological warfare agent. In order to detect abrin in food samples, a two-layer sandwich format enzyme-linked immunosorbent assay based on the monoclonal antibody (mAb) (as capture antibody)
• PMID 22980855

Japanese Journal

• Differential-Mode Biosensor Using Dual Extended-Gate Metal--Oxide--Semiconductor Field-Effect Transistors

• Choi Jinhyeon,Lee Hee Ho,Ahn Jungil [他],Seo Sang-Ho,Shin Jang-Kyoo
• Jpn J Appl Phys 51(6), 06FG05-06FG05-5, 2012-06-25
• … The outputs were measured using a semiconductor parameter analyzer in a phosphate buffered saline (PBS; …
• NAID 150000104200

• Identification of a New IgE-Binding Epitope of Peanut Oleosin That Cross-Reacts with Buckwheat

• KOBAYASHI Shoko,KATSUYAMA Shinta,WAGATSUMA Tamae,OKADA Shinji,TANABE Soichi
• Bioscience, biotechnology, and biochemistry 76(6), 1182-1188, 2012-06-23
• … The phosphate-buffered saline-soluble fraction of buckwheat inhibited the binding between IgE and the peanut allergen. …
• NAID 10030818299

Related Links

• PBS（Phosphate Buffered Salts）タブレット 大容量品｜タカラ ...

Phosphate Buffered Saline (PBS) Tablets, pH7.4（製品コード T9181） Phosphate Buffered Saline (PBS) Tablets without Potassium, pH7.4（製品コード T9182） ・ELISA、ウェスタンブロッテイング、免疫組織染色における洗浄バッファー

• Phosphate buffered saline | Protocols Online

Introduction Phosphate buffered saline (abbreviated as PBS) is a buffer solution commonly used in biological research. It is a salty solution containing sodium chloride, sodium phosphate, and (in some formulations) potassium chloride ...

Related Pictures

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★リンクテーブル★

リンク元	「PBS」「リン酸緩衝食塩水」
関連記事	「phosphate」「saline」「buffer」

「PBS」

　　[★]

• リン酸緩衝食塩水

関

phosphate-buffered saline

「リン酸緩衝食塩水」

　　[★]

英

phosphate-buffered saline、PBS

「phosphate」

　　[★]

• n.

• (化合物)リン酸塩、リン酸エステル、ホスフェート、フォスフェート、リン酸

関

inorganic phosphate、orthophosphate、orthophosphoric acid、phospho、phosphoester、phosphoric、phosphoric acid、phosphoric acid ester、phosphorus

　 　 　

「saline」

　　[★]

• n.

• 生理食塩水、食塩水、食塩液、塩類溶液、生理的食塩水、生食水

• adj.

• 塩類の

関

isotonic sodium chloride solution、physiological saline、saline solution、salt、salt solution

　 　

「buffer」

　　[★]

• 緩衝液、緩衝剤、バッファー、緩衝、緩衝する

関

ampholyte、buffer agent、buffer solution、buffering、buffering solution