免疫放射定量測定法 IRMA
WordNet
- a quantitative or qualitative test of a substance (especially an ore or a drug) to determine its components; frequently used to test for the presence or concentration of infectious agents or antibodies etc.
- analyze (chemical substances)
- a substance that is undergoing an analysis of its components
- an appraisal of the state of affairs; "they made an assay of the contents"; "a check on its dependability under stress" (同)check
- a written report of the results of an analysis of the composition of some substance
- hardy and sure-footed animal smaller and with longer ears than the horse
- a pompous fool
PrepTutorEJDIC
- (金属の品質・鉱石の金属含有量・薬品の)分析,試金 / 試金物,分析物 / …'を'試金する,分析する
- ロバ / ばか者
- 《米俗》しり,けつ(《英俗》arse)
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2016/01/31 12:20:21」(JST)
[Wiki en表示]
Immunoradiometric assay (IRMA) is an assay that uses radiolabeled antibodies. It differs from conventional radioimmunoassay (RIA) in that the compound to be measured combines immediately with the radiolabeled antibodies, rather than displacing another antigen by degrees over some period.
Introduction
Fluorescent and radioactive antibodies have been used to locate or measure solid-phase antigens for many years. However, only recently has the labeled antibody been applied to measurement of antigen to sample. The method converts the unknown antigen into a tracable radioactive product. Immuno radiometric assay (IRMA) was first introduced by "Miles and Hales" in 1968.
Principle
In IRMA, the antibodies are labeled with radioisotopes which are used to bind antigens present in the specimen. When a positive sample is added to the tubes, radioactively labeled (labeled with I125 or I135 radioisotopes) antibodies bind to the free epitopes of antigens and form an antigen-antibody complex. Unbound labeled antibodies are removed by a second reaction with a solid phase antigen. The amount of radioactive remaining in the solution is direct function of the antigen concentration.
Medical tests: Immunologic techniques and tests (CPT 86000–86849)
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Immunologic techniques
and tests
serology/
diagnostic immunology
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Immunoprecipitation |
- Chromatin immunoprecipitation
- Immunodiffusion
- Ouchterlony double immunodiffusion
- Radial immunodiffusion
- Immunoelectrophoresis
- Counterimmunoelectrophoresis
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Immunoassay |
- ELISA
- ELISPOT
- Enzyme Multiplied Immunoassay Technique
- RAST test
- Radioimmunoassay
- Radiobinding assay
- Immunofluorescence
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Agglutination |
- Hemagglutination/Hemagglutinin
- Latex fixation test
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Other |
- Nephelometry
- Complement fixation test
- Immunocytochemistry
- Immunohistochemistry
- Direct fluorescent antibody
- Epitope mapping
- Skin allergy test
- Patch test
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Inflammation |
- C-reactive protein
- Procalcitonin
- Total complement activity
- MELISA
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Index of the immune system
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Description |
- Physiology
- cells
- autoantigens
- autoantibodies
- complement
- surface antigens
- IG receptors
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Disease |
- Allergies
- Immunodeficiency
- Immunoproliferative immunoglobulin disorders
- Hypersensitivity and autoimmune disorders
- Neoplasms and cancer
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Treatment |
- Procedures
- Drugs
- antihistamines
- immunostimulants
- immunosuppressants
- monoclonal antibodies
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UpToDate Contents
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English Journal
- Association of postmenopausal endogenous sex hormones with global methylation level of leukocyte DNA among Japanese women.
- Iwasaki M, Ono H, Kuchiba A, Kasuga Y, Yokoyama S, Onuma H, Nishimura H, Kusama R, Yoshida T, Tsugane S.AbstractABSTRACT:
- BMC cancer.BMC Cancer.2012 Jul 29;12(1):323. [Epub ahead of print]
- ABSTRACT:BACKGROUND: Although global hypomethylation of leukocyte DNA has been associated with an increased risk of several sites of cancer, including breast cancer, determinants of global methylation level among healthy individuals remain largely unexplored. Here, we examined whether postmenopausal
- PMID 22839213
- Follicular fluid concentrations of IGF-I, IGF-II, IGFBP-3, VEGF, AMH, and inhibin-B in women undergoing controlled ovarian hyperstimulation using GnRH agonist or GnRH antagonist.
- Kaya A, Atabekoglu CS, Kahraman K, Taskin S, Ozmen B, Berker B, Sonmezer M.SourceAnkara University School of Medicine, Department of Obstetrics and Gynecology, 06100 Ankara, Turkey.
- European journal of obstetrics, gynecology, and reproductive biology.Eur J Obstet Gynecol Reprod Biol.2012 Jul 7. [Epub ahead of print]
- OBJECTIVE: To compare follicular fluid concentrations of IGF-I, IGF-II, IGFBP-3, inhibin-B, VEGF, and AMH in women undergoing controlled ovarian hyperstimulation with a long-luteal GnRH agonist protocol or multiple-dose GnRH antagonist protocol.STUDY DESIGN: A total of 80 cycles were included; long-
- PMID 22776750
Japanese Journal
- アドレノメデュリン (広範囲 血液・尿化学検査 免疫学的検査(第7版・4)その数値をどう読むか) -- (内分泌学的検査 その他)
- 第30回イムノアッセイ検査全国コントロールサーベイ成績報告要旨(2008年)
- 社団法人 日本アイソトープ協会 医学・薬学部会 インビトロテスト専門委員会イムノアッセイ研究会,家入蒼 生夫,桑 克彦,池田 斉,小田桐 恵美,片上 秀喜,亀子 光明,竹岡 啓子,武田 京子,日高 洋
- Radioisotopes 58(10), 655-708, 2009-10-15
- NAID 10025536418
- 第30回イムノアッセイ検査 全国コントロールサーベイ成績報告要旨(2008年)
- 社団法人 日本アイソトープ協会 医学・薬学部会 インビトロテスト専門委員会 イムノアッセイ研究会
- RADIOISOTOPES 58(10), 655-708, 2009
- 第30回イムノアッセイ検査全国コントロールサーベイ結果を報告した。参加施設数は108,サーベイ対象は,ホルモン,腫瘍マーカー,薬物など35項目であった。non-RI法による参加が約90%を占めた。試料は,市販の管理血清5種類及び20施設に対してはヒトプール血清を同時に配布し,調査した。結果は,全項目キット内・キット間変動ともほぼ例年通りのばらつきを示した。今回と前回とでは測定試料が変わっている項目 …
- NAID 130004802868
Related Links
- im·mu·no·ra·di·o·met·ric as·say an assay that differs from conventional radioimmunoassay in that the compound to be measured combines directly with radioactively labeled antibodies. immunoradiometric assay [-rā′dē·ōmet′rik] a ...
- Immunoradiometric assay information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues. ... Introduction: Immunoradiometric assay Description of Immunoradiometric assay ...
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