|
This article needs more medical references for verification or relies too heavily on primary sources. Please review the contents of the article and add the appropriate references if you can. Unsourced or poorly sourced material may be removed. (December 2012) |
|
Conjugated linoleic acids (CLA) are a family of at least 28[1] isomers of linoleic acid found mostly in the meat and dairy products derived from ruminants. In humans, CLAs are produced from linoleic acid by bacteria of the gastrointestinal tract.[2] CLAs can be either cis- or trans-fats and the double bonds of CLAs are conjugated and separated by a single bond between them.
Contents
- 1 History
- 2 Biochemistry
- 3 Diet and health
- 3.1 Possible beneficial effects of CLA supplements in humans
- 3.2 Possible adverse effects of CLA (supplements) in humans
- 3.3 Dietary sources
- 4 See also
- 5 References
|
History [edit]
In 1979, researchers from the University of Wisconsin applied a beef extract to mice skin. The mice were then exposed to a strong carcinogen. When the researchers counted the number of tumors developed by the mice 16 weeks later, they found to their surprise that the mice exposed to the beef extract had 20% fewer tumors. The identity of this anticarcinogen was not discovered until almost a decade later, in 1987. Michael Pariza, the scientist who discovered CLA, later remarked that "few anticarcinogens, and certainly no other known fatty acids, are as effective as CLA in inhibiting carcinogenesis in these models."[3][4] Although CLA is known for its anticancer properties, researchers have also found that the cis-9, trans-11 form of CLA can reduce the risk for cardiovascular disease and help fight inflammation.[5][6]
The Nutritional Immunology and Molecular Medicine Laboratory (NIMML) demonstrated that oral CLA treatment prevents or ameliorates inflammatory bowel disease by activating the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR gamma).[7] In 2012, NIMML conducted a human clinical trial administering 6 grams/day of a CLA supplement to Crohn's disease patients and reported a remarkable improvement in Crohn's disease activity index and quality of life measures.[8] These clinical improvements paralleled modulation of T cell responses in blood subjects that received CLA supplement.
CLA is also known for its body weight management properties, which include reducing body fat and increasing lean muscle mass. Over 30 clinical studies have been published investigating the effect of CLA on weight management. The trials have quite variable designs, which leads to inconsistency. However a meta-analysis conducted in 2007 concluded CLA has a small impact on fat mass.[9]
The United States Food and Drug Administration categorizes CLA as generally recognized as safe (GRAS) status for certain food categories, including fluid milk, yogurt, meal replacement shakes, nutritional bars, fruit juices and soy milk.[citation needed] With GRAS status, food companies are able to add CLA to products in these food categories.
Biochemistry [edit]
Most studies of CLAs have used a mixture of isomers wherein the isomers c9,t11-CLA and t10,c12-CLA were the most abundant.[10] More recent studies using individual isomers indicate that the two isomers have very different health effects.[5][11]
Conjugated linoleic acid is both a trans fatty acid and a cis fatty acid. The cis bond causes a lower melting point and ostensibly also the observed beneficial health effects. Unlike other trans fatty acids, it may have beneficial effects on human health.[12] CLA is conjugated, and in the United States, trans linkages in a conjugated system are not counted as trans fats for the purposes of nutritional regulations and labeling.[citation needed] CLA and some trans isomers of oleic acid are produced by microorganisms in the rumens of ruminants. Non-ruminants, including humans, produce certain isomers of CLA from trans isomers of oleic acid, such as vaccenic acid, which is converted to CLA by delta-9-desaturase.[13][14]
Diet and health [edit]
Possible beneficial effects of CLA supplements in humans [edit]
Anticancer properties have been attributed to CLA, and studies on mice and rats show encouraging results in hindering the growth of tumors in mammary (except HER2 breast cancer), skin, and colon tissues.[15][16][17][18][19][20][21][22][23][24][25][26][27][28] It has been reported that CLA can up-regulate the tumor suppressor gene PTPRG, and may have anticancer properties.[16][29]
A European team led by the Swiss scientist Lukas Rist has found that mothers consuming mostly organic milk and meat products have about 50% higher levels of rumenic acid in their breast milk.[30]
According to studies that targeted the effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs, the supplemental CLA usage had a positive effect on the improvement of belly firmness and may provide a nutritional solution to carcass fat and belly firmness problems.[31]
The most promising science around CLA concerns its effect on weight management. Thirty-five intervention studies have been conducted using CLA in humans to investigate the effects of CLA on weight management. These studies, which vary widely in CLA dose and duration, show the most significant effect of CLA on weight management is on body composition, a reduction in total body fat and an increase in lean body mass. The effect of CLA on fat mass is modest and at the recommended dosage of 3.2 g/day produces a statistically significant 90 g fat loss per week (about 1 lb in 5 wk) as shown by a 2007 meta-analysis.[32] Doses higher than the recommended 3.2g do not seem to have any additional effects on body fat reduction. Another meta-analysis found that CLA supplementation produces about 1% increased growth in targeted lean body mass per week [33] With the simultaneous decrease in fat mass coupled with increases in lean body mass, often the net change in weight is small. However, the effects of CLA on body composition is a healthy effect, since the degree of fat mass is related to many causes of mortality[34] and lean body mass burns more calories than fat mass, which may help to increase resting metabolic rates. CLA use itself is not an answer to the prevalence of obesity, but it can be a useful tool in addition to a healthy lifestyle and exercise program to achieve and maintain a healthy body weight.
Some studies have found no significant effects of CLA supplementation on fat mass loss.[35][36][37][38][39][40][37][38][39] CLA has also been used in combination with other ingredients which may skew results.[41]
Possible adverse effects of CLA (supplements) in humans [edit]
There are concerns that the use of CLA supplements by extremely overweight people may tend to cause or to aggravate insulin resistance, which may increase their risk of developing diabetes.[11] Commercially available supplements contain equal mixtures of two CLA isomers: the cis-9, trans-11 isomer (also known as rumenic acid, the predominant CLA isomer in milk and beef), as well as the trans-10, cis-12 (t10c12) isomer. All other isomers ratios found in the scientific literature are not commercially available. The trans-10, cis-12 isomer is linked to many adverse side effects. Research indicates that supplementation with t10c12 CLA dramatically increases rates of oxidative stress, to levels considerably higher than that observed in heavy smokers.[11]
However, the evidence is controversial, and some studies using a mixture of c9t11 and t10c12 CLA showed no changes in insulin sensitivity.[42][43] A study in 2007 used the euglycemic hyperinsulinemic clamp method, which is the gold standard, to evaluate insulin resistance. The study performed on 49 obese or overweight individuals taking 3.2g CLA per day for six months found no adverse effects on blood glucose management. In addition, the long-term studies of one and two years have found CLA supplementation to be well tolerated, but there was an increase in some markers associated with inflammation and cardiovascular disease risk, and two of 134 subjects had increased levels of transaminases.[44] In one study, t10c12 CLA produced a 32% increase in biliary cholesterol concentration, which increases the chance of gallstone formation.[45] In 2006, a study by the US Department of Agriculture suggested CLA can induce essential fatty acid redistribution in mice. Changes in docosahexaenoic acid (DHA) and arachidonic acid (AA) levels were observed in some organs. For instance, the t10,c12 CLA reduced the DHA content of heart tissue by 25%, while in the spleen, DHA content rose, and AA fell. DHA is an omega-3 fatty acid important to cardiovascular health, and the dramatic reduction of DHA in heart tissue can have serious health consequences. In contrast, c9,t11 CLA did not alter DHA content in the heart, but did reduce spleen DHA slightly.[10] A study of CLA supplementation (equal amounts of c9,t11 and t10,c12) in hatchling chicks showed high mortality and low hatchability rates among CLA-supplemented groups, and also a decrease in brain DHA levels of CLA-treated chicks.[46] These studies raise the question of whether CLA may increase the risk of cardiovascular and inflammatory diseases, but whether such changes occur in humans and are clinically relevant have yet to be established.
The general consensus is that the use of this supplement should be carefully examined if the person using the supplement is greatly overweight.[47]
A CLA supplement containing the t10,c12 isomer may be deleterious to women whose breast epithelium or tumor overexpresses Her2/ErbB2, as well, potentially, to other women at risk for breast cancer. [48]
Dietary sources [edit]
Kangaroo meat may have the highest concentration of CLA.[49] Food products from grass-fed ruminants (e.g. mutton and beef) are good sources of CLA, and contain much more of it than those from grain-fed animals.[50] In fact, meat and dairy products from grass-fed animals can produce 300-500% more CLA than those of cattle fed the usual diet of 50% hay and silage, and 50% grain.[51]
Eggs from chickens that have been fed CLA are also rich in CLA, and CLA in eggs has been shown to survive the temperatures encountered during frying.[52]
Some mushrooms, such as Agaricus bisporus and Agaricus subrufescens, are rare nonanimal sources of CLA.[53][54] A commercial mixture of CLA, called Tonalin, is sourced from natural safflower oil [55]
See also [edit]
References [edit]
- ^ Banni S (June 2002). "Conjugated linoleic acid metabolism". Curr. Opin. Lipidol. 13 (3): 261–6. doi:10.1097/00041433-200206000-00005. PMID 12045395.
- ^ M. Coakley, R.P. Ross, M. Nordgren, G. Fitzgerald, R. Devery, C. Stanton (January 2003). "Conjugated linoleic acid biosynthesis by human-derived Bifidobacterium species". Journal of Applied Microbiology 94 (1): 138–145. doi:10.1046/j.1365-2672.2003.01814.x. PMID 12492934.
- ^ Ha YL, Grimm NK, Pariza MW (1987). "Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid". Carcinogenesis 8 (12): 1881–7. doi:10.1093/carcin/8.12.1881. PMID 3119246.
- ^ Williams, Lane; Publishing, Woodland (1999-01). CLA: Conjugated Linoleic Acid - Google Book Search. Woodland Publishing. ISBN 978-1-58054-008-7.
- ^ a b Tricon S, Burdge GC, Kew S et al. (September 2004). "Opposing effects of cis-9,trans-11 and trans-10,cis-12 conjugated linoleic acid on blood lipids in most healthy humans". Am. J. Clin. Nutr. 80 (3): 614–20. PMID 15321800.
- ^ Zulet MA, Marti A, Parra MD, Martínez JA (September 2005). "Inflammation and conjugated linoleic acid: mechanisms of action and implications for human health". J. Physiol. Biochem. 61 (3): 483–94. doi:10.1007/BF03168454. PMID 16440602.
- ^ Bassaganya-Riera, J; Reynolds, K; Martino-Catt, S; Cui, Y; Hennighausen, L; Gonzalez, F; Rohrer, J; Benninghoff, AU et al. (2004). "Activation of PPAR gamma and delta by conjugated linoleic acid mediates protection from experimental inflammatory bowel disease". Gastroenterology 127 (3): 777–91. doi:10.1053/j.gastro.2004.06.049. PMID 15362034.
- ^ "Researchers discover novel therapy for Crohn’s disease" (Press release). Marketing and Communications, Virginia Bioinformatics Institute. March 16, 2012.
- ^ Whigham L et al. (January 2007). "Efficacy of conjugated linoleic acid for reducing fat mass:a meta-analysis in humans". Am. J. Clin. Nutr. 85 (5): 1203–11. PMID 17490954.
- ^ a b "Fatty Acid Profiles of Liver, Adipose Tissue, Speen, and Heart of Mice Fed Diets Containing T10, C-12-, and C9, T11-Conjugated Linoleic Adic".
- ^ a b c Ulf Risérus, MMed; Samar Basu, PhD; Stefan Jovinge, MD, PhD; Gunilla Nordin Fredrikson, PhD; Johan Ärnlöv, MD; Bengt Vessby, MD, PhD (September 2002). "Supplementation With Conjugated Linoleic Acid Causes Isomer-Dependent Oxidative Stress and Elevated C-Reactive Protein". American Heart Association Journals 106 (15): 1925–9. doi:10.1161/01.CIR.0000033589.15413.48. PMID 12370214. 01.CIR.0000033589.15413.48v1. Retrieved 2007-02-19.
- ^ II International Congress on CLA from Experimental Models to Human Application
- ^ Kuhnt K, Kraft J, Moeckel P, Jahreis G (April 2006). "Trans-11-18 : 1 is effectively Delta9-desaturated compared with trans-12-18 : 1 in humans". Br J Nutr. 95 (4): 752–761. doi:10.1079/BJN20051680. PMID 16571155.
- ^ Banni S, Angioni E, Murru E, Carta G, Melis M, Bauman D, Dong Y, Ip C (2001). "Vaccenic acid feeding increases tissue levels of conjugated linoleic acid and suppresses development of premalignant lesions in rat mammary gland". Nutr Cancer 41 (1–2): 91–7. doi:10.1207/S15327914NC41-1&2_12 (inactive 2010-09-04). PMID 12094634.
- ^ Belury, M.A. (October 2002). "Inhibition of carcinogenesis by conjugated linoleic acid: Potential mechanisms of action". Journal of Nutrition 132 (10): 2995–2998. PMID 12368384.
- ^ a b Amarù DL, Field CJ. (2009). "Conjugated Linoleic Acid Decreases MCF-7 Human Breast Cancer Cell Growth and Insulin-Like Growth Factor-1 Receptor Levels". Lipids 26 (5): 449–58. doi:10.1007/s11745-009-3288-4. PMID 19266226.
- ^ Lee Y, Thompson JT, de Lera AR, Vanden Heuvel JP. (2008). "Isomer-specific effects of conjugated linoleic acid on gene expression in RAW 264.7". J Nutr Biochem 26 (11): 848–59, 859.e1–5. doi:10.1016/j.jnutbio.2008.07.013. PMID 18993052.
- ^ Coakley M, Banni S, Johnson MC, Mills S, Devery R, Fitzgerald G, Paul Ross R, Stanton C. (2009). "Inhibitory Effect of Conjugated alpha-Linolenic Acid from Bifidobacteria of Intestinal Origin on SW480 Cancer Cells". Lipids 44 (3): 249–56. doi:10.1007/s11745-008-3269-z. PMID 19048324.
- ^ Ip C, Scimeca JA, Thompson HJ. (1994). "Conjugated linoleic acid. A powerful anticarcinogen from animal fat sources". Cancer 74 (3): 1050–4. doi:10.1002/1097-0142(19940801)74:3+<1050::AID-CNCR2820741512>3.0.CO;2-I. PMID 8039138.
- ^ Kritchevsky D. (May 2000). "Antimutagenic and some other effects of conjugated linoleic acid". Br J Nutr. 83 (5): 459–65. PMID 10953669.
- ^ Pariza MW, Park Y, Cook ME. (July 2001). "The biologically active isomers of conjugated linoleic acid". Prog Lipid Res. 40 (4): 283–98. doi:10.1016/S0163-7827(01)00008-X. PMID 11412893.
- ^ Bhattacharya A, Banu J, Rahman M, Causey J, Fernandes G. (December 2006). "Biological effects of conjugated linoleic acids in health and disease". J Nutr Biochem. 17 (12): 789–810. doi:10.1016/j.jnutbio.2006.02.009. PMID 16650752.
- ^ Donnelly C, Olsen AM, Lewis LD, Eisenberg BL, Eastman A, Kinlaw WB. (2009). "Conjugated Linoleic Acid (CLA) inhibits expression of the Spot 14 (THRSP) and fatty acid synthase genes and impairs the growth of human breast cancer and liposarcoma cells". Nutr Cancer. 61 (1): 114–22. doi:10.1080/01635580802348666. PMC 2872989. PMID 19116881.
- ^ Islam MA, Kim YS, Jang WJ, Lee SM, Kim HG, Kim SY, Kim JO, Ha YL. (2008). "A mixture of trans, trans conjugated linoleic acid induces apoptosis in MCF-7 human breast cancer cells with reciprocal expression of Bax and Bcl-2". J Agric Food Chem (Korea) 56 (14): 5970–6. doi:10.1021/jf8004977. PMID 18570428.
- ^ Kim YS, Cerbo RM, Hah CK, Bahn KN, Kim JO, Ha YL. (2008). "Growth inhibition of osteosarcoma cell MG-63 by a mixture of trans,trans conjugated linoleic acid isomers: possible mechanistic actions". J Food Sci. (Korea) 73 (1): T7–15. doi:10.1111/j.1750-3841.2007.00584.x. PMID 18211379.
- ^ Kelley NS, Hubbard NE, Erickson KL. (2007). "Conjugated linoleic acid isomers and cancer". J Nutr (UC Davis, Ca, USA) 137 (12): 2599–607. PMID 18029471.
- ^ Fite A, Goua M, Wahle KW, Schofield AC, Hutcheon AW, Heys SD. (2007). "Potentiation of the anti-tumour effect of docetaxel by conjugated linoleic acids (CLAs) in breast cancer cells in vitro". Prostaglandins Leukot Essent Fatty Acids. (Scotland, UK) 77 (2): 87–96. doi:10.1016/j.plefa.2007.08.004. PMID 17900885.
- ^ Ou L, Ip C, Lisafeld B, Ip MM. (2007). "Conjugated linoleic acid induces apoptosis of murine mammary tumor cells via Bcl-2 loss". Biochem Biophys Res Commun. (NY, USA) 356 (4): 1044–9. doi:10.1016/j.bbrc.2007.03.096. PMC 1992442. PMID 17400188.
- ^ Wang LS, Huang YW, Sugimoto Y (2006), "Conjugated linoleic acid (CLA) up-regulates the estrogen-regulated cancer suppressor gene, protein tyrosine phosphatase {gamma} (PTP{gamma}), in human breast cells.", Anticancer Res 26: 27–34, PMID 16475675
- ^ Lukas Rist, Andre Mueller, Christiane Barthel, Bianca Snijders, Margje Jansen, A. Paula Simoes-Wust, Machteld Huber, Ischa Kummeling, Ursula von Mandach, Hans Steinhart, and Carel Thijs. (June 2007). "Influence of organic diet on the amount of conjugated linoleic acids in breast milk". British Journal of Nutrition.
- ^ S. T. Larsen, B. R. Wiegand,2, F. C. Parrish, Jr., J. E. Swan and J. C. Sparks (2009). "Dietary conjugated linoleic acid changes belly and bacon quality from pigs fed varied lipid sources". Journal of Animal Science (American Society of Animal Science) 87 (1): 285–295. doi:10.2527/jas.2008-1213. PMID 18820159
- ^ Whingham LD, Watras CA, Scholler DA (2007). "Efficacy of conjugated linoleic acid for reducing fat mass: a meta-analysis in humans. Am". J Clin Nutr 85 (5): 1203–1200.
- ^ Steck SE, Chalecki AM, Miller P, Conway J, Austin GL, Hardin JW, Albright CD, Thuillier P. (2007). "Conjugated linoleic acid supplementation for twelve weeks increases lean body mass in obese humans.". J Nutr. 137 (5): 1188–93. PMID 17449580.
- ^ Heitmann BL, Erikson H, Ellsinger BM, Mikkelsen KL, Larrson B (2000). "Mortality associated with body fat, fat-free mass and body mass index among 60-year-old swedish men - a 22-year follow-up. The study of men born in 1913". Int J Obes Relat Metab Disord 24 (1): 33–37. doi:10.1038/sj.ijo.0801082. PMID 10702748.
- ^ Lambert EV, Goedecke JH, Bluett K, Heggie K, Claassen A, Rae DE, West S, Dugas J, Dugas L (2007). "Conjugated linoleic acid versus high-oleic acid sunflower oil: effects on energy metabolim, glucose tolerance, blood lipids, appetite and body composition in regularly exercising individuals". Br J Nutr 97 (5): 1001–1011. doi:10.1017/S0007114507172822. PMID 17381964.
- ^ Nazare JA, Perriere AB, Bonnet F, Desage M, Peyrat J, Maitrepierre C, Louche-Pelissier C, Bruzeau J, Goudable J (2007). "Daily intake of conjugated linoleic acid-enriched yoghurts:effects on energy metabolism and adipose tissue gene expression in healthy subjects". Br J Nutr 97 (2): 273–280. doi:10.1017/S0007114507191911. PMID 17298695.
- ^ a b Petridou A, Mougios V, Sagredos A (2003). "Supplementation with CLA: isomer incorporation into serum lipids and effect on body fat of women". Lipids 38 (8): 805–11. doi:10.1007/s11745-003-1129-2. PMID 14577658.
- ^ a b * Berven G, Amund B, Ottar H, Blankson H, Fagertun H, Thom E, Wadstein J, Gudmundson O (2000). "Safety of conjugated linoleic acid (CLA) in overweight or obese human volunteers". Eur J Lipid Sci Technol 102 (7): 455–462. doi:10.1002/1438-9312(200008)102:7<455::AID-EJLT455>3.0.CO;2-V.
- ^ a b * Whigham LD, O'Shea M, Mohede IC, Walaski HP, Atkinson RL (2004). "Safety profile of conjugated linoleic acids in a 12-month trial in obese humans". Food Chem Toxicol 42 (10): 1701–9. doi:10.1016/j.fct.2004.06.008. PMID 15354322.
- ^ Atkinson RL et al. Conjugated linoleic acid for altering body composition and treating obesity. In: Advances in conjugated linoleic acid research, Vol. 1. Eds M.P. Yurawecz, M.M Mossoba, J.K.G. Kramer, M.W. Pariza, G.J. Nelson, AOCS Press, Champaign Illinois (USA) 1999, pp. 384-35.
- ^ * Diaz ML, Watkins BA, Li Y, Anderson RA, Campbell WW (2008). "Chromium pocolinate and conjugated linoleic acid do not synergistically influence diet- and exercise-induced changes in body composition and health indexes in overweight women". J Nutr Biochem 19 (1): 61–68. doi:10.1016/j.jnutbio.2007.01.006. PMID 17531459.
- ^ Watras, AC; Buchholz, AC; Close, RN; Zhang, Z; Schoeller, DA (2006-08-22). "The role of conjugated linoleic acid in reducing body fat and preventing holiday weight gain". International Journal of Obesity 31 (3): 481–7. doi:10.1038/sj.ijo.0803437. PMID 16924272. Retrieved 2008-09-30.
- ^ Syvertsen, C; Halse, J; Høivik, HO; Gaullier, JM; Nurminiemi, M; Kristiansen, K; Einerhand, A; O'Shea, M et al. (2006-10-10). "The effect of 6 months supplementation with conjugated linoleic acid on insulin resistance in overweight and obese". International Journal of Obesity 31 (7): 1148–54. doi:10.1038/sj.ijo.0803482. PMID 17031391. Retrieved 2008-09-30.
- ^ Gaullier JM, Hals J, Hoye K, Kristiansen K, Fagertun H, Vik H, Gudmundsen O (2005). "Supplementation with conjugated linoleic acid for 24 months is well tolerated by and reduces body fat mass in healthy, overweight humans". J Nutr 135 (4): 778–84. PMID 15795434.
- ^ 33.^ * Nazare JA, Perriere AB, Bonnet F, Desage M, Peyrat J, Maitrepierre C, Louche-Pelissier C, Bruzeau J, Goudable J (2007). "Daily intake of conjugated linoleic acid-enriched yoghurts:effects on energy metabolism and adipose tissue gene expression in healthy subjects". Br J Nutr 97 (2): 273–280. doi:10.1017/S0007114507191911. PMID 17298695.
- ^ Cherian, G.; Ai, W.; Goeger, M. P. (2005). "Maternal dietary conjugated linoleic acid alters hepatic triacylglycerol and tissue fatty acids in hatched chicks". Lipids 40 (2): 131–136. PMID 15884760. edit
- ^ "Weight Loss & Diet Plans". WebMD.
- ^ Meng X, Shoemaker SF, McGee SO, Ip MM. (2008). "t10,c12-Conjugated linoleic acid stimulates mammary tumor progression in Her2/ErbB2 mice through activation of both proliferative and survival pathways". Carcinogenesis (NY, USA) 29 (5): 1013–21. doi:10.1093/carcin/bgn035. PMC 2777529. PMID 18339686.
- ^ "Kangaroo meat - health secret revealed" (Press release). Commonwealth Scientific and Industrial Research Organisation (CSIRO). 2004-04-23. Retrieved 2007-01-23.
- ^ T. R. Dhiman, L. D. Satter, M. W. Pariza, M. P. Galli, K. Albright, and M. X. Tolosa (1 May 2000). "Conjugated Linoleic Acid (CLA) Content of Milk from Cows Offered Diets Rich in Linoleic and Linolenic Acid". Journal of Dairy Science 83 (5): 1016–1027. doi:10.3168/jds.S0022-0302(00)74966-6. PMID 10821577. Retrieved 2006-05-27.
- ^ T. R. Dhiman (2001). "Role of diet on conjugated linoleic acid content of milk and meat" (PDF). Journal of Animal Science 79. Retrieved 2007-03-09.
- ^ Lin Yang, Ying Cao, Zhen-Yu Chen (2004). "Stability of conjugated linoleic acid isomers in egg yolk lipids during frying". Food Chemistry (Elsevier) 86 (4): 531–535. doi:10.1016/j.foodchem.2003.09.006.
- ^ Chen, S.; Oh, SR; Phung, S; Hur, G; Ye, JJ; Kwok, SL; Shrode, GE; Belury, M et al. (2006). "Anti-aromatase activity of phytochemicals in white button mushrooms (Agaricus bisporus)". Cancer Res. 66 (24): 12026–12034. doi:10.1158/0008-5472.CAN-06-2206. PMID 17178902.
- ^ W. J. Jang S. W. Hyung. Production of natural c9,t11 conjugated linoleic acid (c9,t11 CLA) by submerged liquid culture of mushrooms. Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.
- ^ http://www.tonalin.com/about.htm Retrieved 2013 - 04 - 07