心筋幹細胞

WordNet

grow out of, have roots in, originate in; "The increase in the national debt stems from the last war"
stop the flow of a liquid; "staunch the blood flow"; "stem the tide" (同)stanch, staunch, halt
remove the stem from; "for automatic natural language processing, the words must be stemmed"
the tube of a tobacco pipe
cause to point inward; "stem your skis"
small room in which a monk or nun lives (同)cubicle
a device that delivers an electric current as the result of a chemical reaction (同)electric cell
a room where a prisoner is kept (同)jail cell, prison cell
(biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals
any small compartment; "the cells of a honeycomb"
a small unit serving as part of or as the nucleus of a larger political movement (同)cadre
of or relating to the heart; "cardiac arrest"
the opening into the stomach and that part of the stomach connected to the esophagus

PrepTutorEJDIC

(草の)『茎』,(木の)『幹』 / 葉柄,花梗(かこう) / 『茎状のもの』;(杯・グラスの)『脚』,(パイプ・さじの)柄,(時計の)りゅうず / (単語の)語幹,語根 / 船首;船首材 / …‘の'茎(軸)を取り去る,へたをとる / (…から)生じる,(…に)由来する《+『from』+『名』(do『ing』)》
〈流れなど〉‘を'止める / 〈風・水流など〉‘に'逆らって進む,抵抗する / 〈攻撃・反対など〉‘を'くい止める,押さえる
(刑務所の)『独房』;(修道院の)小さい独居室 / (ミツバチの)みつ房,巣穴 / 小さい部屋 / 『細胞』 / 電池 / 花粉室 / (共産党などの)細胞
心臓の

UpToDate Contents

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1. ST上昇型心筋梗塞の非急性期マネージメントの概要 overview of the non acute management of st elevation myocardial infarction
2. 幹細胞の概要 overview of stem cells
3. 心不全に対する試験中および新規の治療 investigational and emerging therapies for heart failure
4. 造血幹細胞の概要 overview of hematopoietic stem cells
5. 造血幹細胞の分布 sources of hematopoietic stem cells

English Journal

Cardiac Fibroblasts Support Endothelial Cell Proliferation and Sprout Formation but not the Development of Multicellular Sprouts in a Fibrin Gel Co-Culture Model.

Twardowski RL1, Black LD 3rd.Author information 1Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02155, USA.AbstractA primary impediment to cardiac tissue engineering lies in the inability to adequately vascularize the constructs to optimize survival upon implantation. During normal angiogenesis, endothelial cells (ECs) require a support cell to form mature patent lumens and it has been demonstrated that pericytes, vascular smooth muscle cells and mesenchymal stem cells (MSCs) are all able to support the formation of mature vessels. In the heart, cardiac fibroblasts (CFs) provide important electrical and mechanical functions, but to date have not been sufficiently studied for their role in angiogenesis. To study CFs role in angiogenesis, we co-cultured different concentrations of various cell types in fibrin hemispheres with appropriate combinations of their specific media, to determine the optimal conditions for EC growth and sprout formation through DNA analysis, flow cytometry and immunohistology. ECs proliferated best when co-cultured with CFs and analysis of immunohistological images demonstrated that ECs formed the longest and most numerous sprouts with CFs as compared to MSCs. However, ECs were able to produce more multicellular sprouts when in culture with the MSCs. Moreover, these effects were dependent on the ratio of support cell to EC in co-culture. Overall, CFs provide a good support system for EC proliferation and sprout formation; however, MSCs allow for more multicellular sprouts, which is more indicative of the in vivo process.
Annals of biomedical engineering.Ann Biomed Eng.2014 May;42(5):1074-84. doi: 10.1007/s10439-014-0971-2. Epub 2014 Jan 17.
A primary impediment to cardiac tissue engineering lies in the inability to adequately vascularize the constructs to optimize survival upon implantation. During normal angiogenesis, endothelial cells (ECs) require a support cell to form mature patent lumens and it has been demonstrated that pericyte
PMID 24435656

Micropattern width dependent sarcomere development in human ESC-derived cardiomyocytes.

Salick MR1, Napiwocki BN2, Sha J3, Knight GT2, Chindhy SA4, Kamp TJ5, Ashton RS2, Crone WC6.Author information 1Wisconsin Institutes for Discovery, 330 N Orchard St, Madison, WI 53715, USA; Department of Engineering Physics, University of Wisconsin - Madison, 1500 Engineering Drive, Madison, WI 53706, USA; Materials Science Program, University of Wisconsin - Madison, 1509 University Ave, Madison, WI 53706, USA.2Wisconsin Institutes for Discovery, 330 N Orchard St, Madison, WI 53715, USA; Department of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA.3Wisconsin Institutes for Discovery, 330 N Orchard St, Madison, WI 53715, USA; School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai, China.4Department of Medicine, School of Medicine and Public Health, University of Wisconsin - Madison, 750 Highland Ave, Madison, WI 53706, USA.5Wisconsin Institutes for Discovery, 330 N Orchard St, Madison, WI 53715, USA; Department of Medicine, School of Medicine and Public Health, University of Wisconsin - Madison, 750 Highland Ave, Madison, WI 53706, USA; WiCell Institute, 614 Walnut Street, Madison, WI 53726, USA; Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin - Madison, 1300 University Ave, Madison, WI 53706, USA.6Wisconsin Institutes for Discovery, 330 N Orchard St, Madison, WI 53715, USA; Department of Engineering Physics, University of Wisconsin - Madison, 1500 Engineering Drive, Madison, WI 53706, USA; Materials Science Program, University of Wisconsin - Madison, 1509 University Ave, Madison, WI 53706, USA; Department of Biomedical Engineering, University of Wisconsin - Madison, 1550 Engineering Drive, Madison, WI 53706, USA. Electronic address: crone@engr.wisc.edu.AbstractIn this study, human embryonic stem cell-derived cardiomyocytes were seeded onto controlled two-dimensional micropatterned features, and an improvement in sarcomere formation and cell alignment was observed in specific feature geometries. High-resolution photolithography techniques and microcontact printing were utilized to produce features of various rectangular geometries, with areas ranging from 2500 μm(2) to 160,000 μm(2). The microcontact printing method was used to pattern non-adherent poly(ethylene glycol) regions on gold coated glass slides. Matrigel and fibronectin extracellular matrix (ECM) proteins were layered onto the gold-coated glass slides, providing a controlled geometry for cell adhesion. We used small molecule-based differentiation and an antibiotic purification step to produce a pure population of immature cardiomyocytes from H9 human embryonic stem cells (hESCs). We then seeded this pure population of human cardiomyocytes onto the micropatterned features of various sizes and observed how the cardiomyocytes remodeled their myofilament structure in response to the feature geometries. Immunofluorescence was used to measure α-actinin expression, and phalloidin stains were used to detect actin presence in the patterned cells. Analysis of nuclear alignment was also used to determine how cell direction was influenced by the features. The seeded cells showed clear alignment with the features, dependent on the width rather than the overall aspect ratio of the features. It was determined that features with widths between 30 μm and 80 μm promoted highly aligned cardiomyocytes with a dramatic increase in sarcomere alignment relative to the long axis of the pattern. This creation of highly-aligned cell aggregates with robust sarcomere structures holds great potential in advancing cell-based pharmacological studies, and will help researchers to understand the means by which ECM geometries can affect myofilament structure and maturation in hESC-derived cardiomyocytes.
Biomaterials.Biomaterials.2014 May;35(15):4454-64. doi: 10.1016/j.biomaterials.2014.02.001. Epub 2014 Feb 28.
In this study, human embryonic stem cell-derived cardiomyocytes were seeded onto controlled two-dimensional micropatterned features, and an improvement in sarcomere formation and cell alignment was observed in specific feature geometries. High-resolution photolithography techniques and microcontact
PMID 24582552

Oscillatory shear stress created by fluid pulsatility versus flexed specimen configurations.

Salinas M1, Schmidt DE, Libera M, Lange RR, Ramaswamy S.Author information 1a Tissue Engineered Mechanics, Imaging and Materials (TEMIM) Laboratory, Department of Biomedical Engineering , Florida International University , Miami , FL , USA.AbstractOscillatory shear stress (OSS), caused by time-varying flow environments, may play a critical role in the production of engineered tissue by bone marrow-derived stem cells. This is particularly relevant in heart valve tissue engineering (HVTE), owing to the intense haemodynamic environments that surround native valves. In this study, we examined and quantified the role that (i) physiologically relevant scales of pulsatility and (ii) changes in geometry as a function of specimen flexure have in creating OSS conditions. A U-shaped bioreactor capable of producing flow, stretch and flexure was modelled with housed specimens, and computational fluid dynamic simulations were performed. We found that physiologically relevant OSS can be maximised by the application of pulsatile flow to straight, non-moving specimens in a uniform manner. This finding reduces a substantial layer of complexity in dynamic HVTE protocols in which traditionally, time-varying flow has been promoted through specimen movement in custom-made bioreactors.
Computer methods in biomechanics and biomedical engineering.Comput Methods Biomech Biomed Engin.2014 May;17(7):728-39. doi: 10.1080/10255842.2012.715157. Epub 2012 Aug 24.
Oscillatory shear stress (OSS), caused by time-varying flow environments, may play a critical role in the production of engineered tissue by bone marrow-derived stem cells. This is particularly relevant in heart valve tissue engineering (HVTE), owing to the intense haemodynamic environments that sur
PMID 22920330