WordNet

a substance from which another substance is formed (especially by a metabolic reaction)
a person who goes before or announces the coming of another (同)forerunner
any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein"
(pathology) a waxy translucent complex protein resembling starch that results from degeneration of tissue
a non-nitrogenous food substance consisting chiefly of starch; any substance resembling starch

PrepTutorEJDIC

(…の)前朕,前触れ《+『of』+『名』》
蛋白(たんばく)質

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/02/13 20:04:14」(JST)

wiki en

The metal-binding domain of APP with a bound copper ion. The side chains of the two histidine and one tyrosine residues that play a role in metal coordination are shown in the Cu(I) bound, Cu(II) bound, and unbound conformations, which differ by only small changes in orientation.

The extracellular E2 domain, a dimeric coiled coil and one of the most highly conserved regions of the protein from Drosophila to humans. This domain, which resembles the structure of spectrin, is thought to bind heparan sulfate proteoglycans.^[2]

(a) A low magnification image immediately after co-injection of red negatively charged and green glycine-conjugated beads showing the injection site, marked with an oil droplet, appearing as a round yellow sphere. Overlap of red and green fluorescence produces a yellow image. (b) At 50 min after injection, the red carboxylated beads have progressed in the anterograde direction (to the right) while the green glycine-conjugated beads have made no progress. (c)–(e) An axon co-injected with red APP-C beads and green glycine beads and imaged for 100 frames at 4 s intervals at 40× magnification. (c) Red channel (left) first frame; (center) 50 frames superimposed; (right) all 100 frames superimposed. Note the progression of individual beads towards the right, anterograde, side of the injection site heading towards the presynaptic terminal. (d) Two images of the green channel from the same video sequence; (left) first frame; (center) 100 frames superimposed. Note the lack of significant movement of the green glycine beads. (e) Both red and green channels from 100 frames superimposed of the same video as in (c) and (d). (f) Single bead trajectories at high magnification from a set of superimposed frames showing movements of beads.

Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation,^[3] neural plasticity^[4] and iron export.^[5] APP is best known as the precursor molecule whose proteolysis generates beta amyloid (Aβ), a polypeptide containing 37 to 49 amino acid residues whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients.

Genetics

APP is an ancient and highly conserved protein.^[6] In humans, the gene for APP is located on chromosome 21 and contains 18 exons spaning 290 kilobases.^[7]^[8] Several alternative splicing isoforms of APP have been observed in humans, ranging in length from 365 to 770 amino acids, with certain isoforms preferentially expressed in neurons; changes in the neuronal ratio of these isoforms have been associated with Alzheimer's disease.^[9] Homologous proteins have been identified in other organisms such as Drosophila (fruit flies), C. elegans (roundworms), and all mammals.^[10] The amyloid beta region of the protein, located in the membrane-spanning domain, is not well conserved across species and has no obvious connection with APP's native-state biological functions.^[10]

Mutations in critical regions of Amyloid Precursor Protein, including the region that generates amyloid beta (Aβ), cause familial susceptibility to Alzheimer's disease.^[11]^[12]^[13] For example, several mutations outside the Aβ region associated with familial Alzheimer's have been found to dramatically increase production of Aβ.^[14]

A mutation (A673T) in the APP gene protects against Alzheimer’s disease. This substitution is adjacent to the beta secretase cleavage site and results in a 40% reduction in the formation of amyloid beta in vitro.^[15]

Structure

A number of distinct, largely independently-folding structural domains have been identified in the APP sequence. The extracellular region, much larger than the intracellular region, is divided into the E1 and E2 domains, linked by an acidic domain (AcD); E1 contains two subdomains including a growth factor-like domain (GFLD) and a copper-binding domain (CuBD) interacting tightly together.^[16] A serine protease inhibitor domain, absent from the isoform differentially expressed in the brain, is found between acidic region and E2 domain.^[17] The complete crystal structure of APP has not yet been solved; however, individual domains have been successfully crystallized, the growth factor-like domain,^[18] the copper-binding domain,^[19] the complete E1 domain^[16] and the E2 domain.^[2]

Post-translational processing

APP undergoes extensive post-translational modification including glycosylation, phosphorylation, sialylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments.^[20] It is commonly cleaved by proteases in the secretase family; alpha secretase and beta secretase both remove nearly the entire extracellular domain to release membrane-anchored carboxy-terminal fragments that may be associated with apoptosis.^[10] Cleavage by gamma secretase within the membrane-spanning domain after beta-secretase cleavage generates the amyloid-beta fragment; gamma secretase is a large multi-subunit complex whose components have not yet been fully characterized, but include presenilin, whose gene has been identified as a major genetic risk factor for Alzheimer's.^[21]

The amyloidogenic processing of APP has been linked to its presence in lipid rafts. When APP molecules occupy a lipid raft region of membrane, they are more accessible to and differentially cleaved by beta secretase, whereas APP molecules outside a raft are differentially cleaved by the non-amyloidogenic alpha secretase.^[22] Gamma secretase activity has also been associated with lipid rafts.^[23] The role of cholesterol in lipid raft maintenance has been cited as a likely explanation for observations that high cholesterol and apolipoprotein E genotype are major risk factors for Alzheimer's disease.^[24]

Biological function

Although the native biological role of APP is of obvious interest to Alzheimer's research, thorough understanding has remained elusive.

Synaptic formation and repair

The most-substantiated role for APP is in synaptic formation and repair;^[3] its expression is upregulated during neuronal differentiation and after neural injury. Roles in cell signaling, long-term potentiation, and cell adhesion have been proposed and supported by as-yet limited research.^[10] In particular, similarities in post-translational processing have invited comparisons to the signaling role of the surface receptor protein Notch.^[25]

APP knockout mice are viable and have relatively minor phenotypic effects including impaired long-term potentiation and memory loss without general neuron loss.^[26] On the other hand, transgenic mice with upregulated APP expression have also been reported to show impaired long-term potentiation.^[27]

The logical inference is that because Aβ accumulates excessively in Alzheimer's disease its precursor, APP, would be elevated as well. However, neuronal cell bodies contain less APP as a function of their proximity to amyloid plaques.^[28] The data indicate that this deficit in APP results from a decline in production rather than an increase in catalysis. Loss of a neuron's APP may affect physiological deficits that contribute to dementia.

Anterograde neuronal transport

Molecules synthesized in the cell bodies of neurons must be conveyed outward to the distal synapses. This is accomplished via fast anterograde transport. It has been found that APP can mediate interaction between cargo and kinesin and thus facilitate this transport. Specifically, a short peptide 15-amino-acid sequence from the cytoplasmic carboxy-terminus is necessary for interaction with the motor protein.^[29]

Additionally, it has been shown that the interaction between APP and kinesin is specific to the peptide sequence of APP.^[30] In a recent experiment involving transport of peptide-conjugated colored beads, controls were conjugated to a single amino acid, glycine, such that they display the same terminal carboxylic acid group as APP without the intervening 15-amino-acid sequence mentioned above. The control beads were not motile, which demonstrated that the terminal COOH moiety of peptides is not sufficient to mediate transport.

Iron export

A different perspective on Alzheimer's is revealed by a mouse study that has found that APP possesses ferroxidase activity similar to ceruloplasmin, facilitating iron export through interaction with ferroportin; it seems that this activity is blocked by zinc trapped by accumulated Aβ in Alzheimer's.^[5] It has been shown that a single nucleotide polymorphism in the 5'UTR of APP mRNA can disrupt its translation.^[31]

The hypothesis that APP has ferroxidase activity in its E2 domain and facilitates export of Fe(II) is possibly incorrect since the proposed ferroxidase site of APP located in the E2 domain does not have ferroxidase activity.^[32]^[33]

As APP does not possess ferroxidase activity within its E2 domain, the mechanism of APP-modulated iron efflux from ferroportin has come under scrutiny. One model suggests that APP acts to stabilize the iron efflux protein ferroportin in the plasma membrane of cells thereby increasing the total number of ferroportin molecules at the membrane. These iron-transporters can then be activated by known mammalian ferroxidases (i.e. ceruloplasmin or hephaestin).^[34]

Hormonal regulation

The amyloid-β precursor protein (AβPP) and all associated secretases are expressed early in development and plays a key role in the endocrinology of reproduction – with the differential processing of AβPP by secretases regulating human embryonic stem cell (hESC) proliferation as well as their differentiation into neural precursor cells (NPC). The pregnancy hormone human chorionic gonadotropin (hCG) increases AβPP expression^[35] and hESC proliferation while progesterone directs AβPP processing towards the non-amyloidogenic pathway, which promotes hESC differentiation into NPC.^[36]^[37]^[38]

AβPP and its cleavage products do not promote the proliferation and differentiation of post-mitotic neurons; rather, the overexpression of either wild-type or mutant AβPP in post-mitotic neurons induces apoptotic death following their re-entry into the cell cycle.^[39] It is postulated that the loss of sex steroids (including progesterone) but the elevation in luteinizing hormone, the adult equivalent of hCG, post-menopause and during andropause drives amyloid-β production^[40] and re-entry of post-mitotic neurons into the cell cycle.

Interactions

Amyloid precursor protein has been shown to interact with:

APBA1,^[41]^[42]
APBA2,^[41]^[43]^[44]
APBA3,^[41]^[45]
APBB1,^[42]^[46]^[47]^[48]^[49]
APPBP1,^[50]
APPBP2,^[51]
BCAP31,^[52]
BLMH^[53]
CLSTN1,^[43]^[54]
CAV1,^[55]
COL25A1,^[56]
FBLN1,^[57]
GSN,^[58]
HSD17B10,^[59] and
SHC1.^[60]

APP interacts with reelin, a protein implicated in a number of brain disorders, including Alzheimer's disease.^[61]

References

^ Hynes TR, Randal M, Kennedy LA, Eigenbrot C, Kossiakoff AA (Oct 1990). "X-ray crystal structure of the protease inhibitor domain of Alzheimer's amyloid beta-protein precursor". Biochemistry 29 (43): 10018–22. doi:10.1021/bi00495a002. PMID 2125487.
^ ^a ^b PDB: 1RW6; Wang Y, Ha Y (Aug 2004). "The X-ray structure of an antiparallel dimer of the human amyloid precursor protein E2 domain". Molecular Cell 15 (3): 343–53. doi:10.1016/j.molcel.2004.06.037. PMID 15304215.
^ ^a ^b Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J (Jul 2006). "Synapse formation and function is modulated by the amyloid precursor protein". The Journal of Neuroscience 26 (27): 7212–21. doi:10.1523/JNEUROSCI.1450-06.2006. PMID 16822978.
^ Turner PR, O'Connor K, Tate WP, Abraham WC (May 2003). "Roles of amyloid precursor protein and its fragments in regulating neural activity, plasticity and memory". Progress in Neurobiology 70 (1): 1–32. doi:10.1016/S0301-0082(03)00089-3. PMID 12927332.
^ ^a ^b Duce JA, Tsatsanis A, Cater MA, James SA, Robb E, Wikhe K, Leong SL, Perez K, Johanssen T, Greenough MA, Cho HH, Galatis D, Moir RD, Masters CL, McLean C, Tanzi RE, Cappai R, Barnham KJ, Ciccotosto GD, Rogers JT, Bush AI (Sep 2010). "Iron-export ferroxidase activity of β-amyloid precursor protein is inhibited by zinc in Alzheimer's disease". Cell 142 (6): 857–67. doi:10.1016/j.cell.2010.08.014. PMC 2943017. PMID 20817278.
^ Tharp WG, Sarkar IN (April 2013). "Origins of amyloid-β". BMC Genomics 14 (1): 290. doi:10.1186/1471-2164-14-290. PMID 23627794.
^ Yoshikai S, Sasaki H, Doh-ura K, Furuya H, Sakaki Y (Mar 1990). "Genomic organization of the human amyloid beta-protein precursor gene". Gene 87 (2): 257–63. doi:10.1016/0378-1119(90)90310-N. PMID 2110105.
^ Lamb BT, Sisodia SS, Lawler AM, Slunt HH, Kitt CA, Kearns WG, Pearson PL, Price DL, Gearhart JD (Sep 1993). "Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic mice [corrected]". Nature Genetics 5 (1): 22–30. doi:10.1038/ng0993-22. PMID 8220418.
^ Matsui T, Ingelsson M, Fukumoto H, Ramasamy K, Kowa H, Frosch MP, Irizarry MC, Hyman BT (Aug 2007). "Expression of APP pathway mRNAs and proteins in Alzheimer's disease". Brain Research 1161: 116–23. doi:10.1016/j.brainres.2007.05.050. PMID 17586478.
^ ^a ^b ^c ^d Zheng H, Koo EH (2006). "The amyloid precursor protein: beyond amyloid". Molecular Neurodegeneration 1 (1): 5. doi:10.1186/1750-1326-1-5. PMC 1538601. PMID 16930452.
^ Goate A, Chartier-Harlin MC, Mullan M, Brown J, Crawford F, Fidani L, Giuffra L, Haynes A, Irving N, James L (Feb 1991). "Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease". Nature 349 (6311): 704–6. doi:10.1038/349704a0. PMID 1671712.
^ Murrell J, Farlow M, Ghetti B, Benson MD (Oct 1991). "A mutation in the amyloid precursor protein associated with hereditary Alzheimer's disease". Science 254 (5028): 97–9. doi:10.1126/science.1925564. PMID 1925564.
^ Chartier-Harlin MC, Crawford F, Houlden H, Warren A, Hughes D, Fidani L, Goate A, Rossor M, Roques P, Hardy J (Oct 1991). "Early-onset Alzheimer's disease caused by mutations at codon 717 of the beta-amyloid precursor protein gene". Nature 353 (6347): 844–6. doi:10.1038/353844a0. PMID 1944558.
^ Citron M, Oltersdorf T, Haass C, McConlogue L, Hung AY, Seubert P, Vigo-Pelfrey C, Lieberburg I, Selkoe DJ (Dec 1992). "Mutation of the beta-amyloid precursor protein in familial Alzheimer's disease increases beta-protein production". Nature 360 (6405): 672–4. doi:10.1038/360672a0. PMID 1465129.
^ Jonsson T, Atwal JK, Steinberg S, Snaedal J, Jonsson PV, Bjornsson S, Stefansson H, Sulem P, Gudbjartsson D, Maloney J, Hoyte K, Gustafson A, Liu Y, Lu Y, Bhangale T, Graham RR, Huttenlocher J, Bjornsdottir G, Andreassen OA, Jönsson EG, Palotie A, Behrens TW, Magnusson OT, Kong A, Thorsteinsdottir U, Watts RJ, Stefansson K (Aug 2012). "A mutation in APP protects against Alzheimer's disease and age-related cognitive decline". Nature 488 (7409): 96–9. doi:10.1038/nature11283. PMID 22801501. Lay summary – The New York Times.
^ ^a ^b Dahms SO, Hoefgen S, Roeser D, Schlott B, Gührs KH, Than ME (Mar 2010). "Structure and biochemical analysis of the heparin-induced E1 dimer of the amyloid precursor protein". Proceedings of the National Academy of Sciences of the United States of America 107 (12): 5381–6. doi:10.1073/pnas.0911326107. PMC 2851805. PMID 20212142. ; see also PDB ID 3KTM
^ Sisodia SS, Koo EH, Hoffman PN, Perry G, Price DL (Jul 1993). "Identification and transport of full-length amyloid precursor proteins in rat peripheral nervous system". The Journal of Neuroscience 13 (7): 3136–42. PMID 8331390.
^ Rossjohn J, Cappai R, Feil SC, Henry A, McKinstry WJ, Galatis D, Hesse L, Multhaup G, Beyreuther K, Masters CL, Parker MW (Apr 1999). "Crystal structure of the N-terminal, growth factor-like domain of Alzheimer amyloid precursor protein". Nature Structural Biology 6 (4): 327–31. doi:10.1038/7562. PMID 10201399. ; see also PDB ID 1MWP
^ Kong GK, Adams JJ, Harris HH, Boas JF, Curtain CC, Galatis D, Masters CL, Barnham KJ, McKinstry WJ, Cappai R, Parker MW (Mar 2007). "Structural studies of the Alzheimer's amyloid precursor protein copper-binding domain reveal how it binds copper ions". Journal of Molecular Biology 367 (1): 148–61. doi:10.1016/j.jmb.2006.12.041. PMID 17239395. ; See also 2007 PDB IDs 2FJZ, 2FK2, 2FKL.
^ De Strooper B, Annaert W (Jun 2000). "Proteolytic processing and cell biological functions of the amyloid precursor protein". Journal of Cell Science 113 (11): 1857–70. PMID 10806097.
^ Chen F, Hasegawa H, Schmitt-Ulms G, Kawarai T, Bohm C, Katayama T, Gu Y, Sanjo N, Glista M, Rogaeva E, Wakutani Y, Pardossi-Piquard R, Ruan X, Tandon A, Checler F, Marambaud P, Hansen K, Westaway D, St George-Hyslop P, Fraser P (Apr 2006). "TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity". Nature 440 (7088): 1208–12. doi:10.1038/nature04667. PMID 16641999.
^ Ehehalt R, Keller P, Haass C, Thiele C, Simons K (Jan 2003). "Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts". The Journal of Cell Biology 160 (1): 113–23. doi:10.1083/jcb.200207113. PMC 2172747. PMID 12515826.
^ Vetrivel KS, Cheng H, Lin W, Sakurai T, Li T, Nukina N, Wong PC, Xu H, Thinakaran G (Oct 2004). "Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes". The Journal of Biological Chemistry 279 (43): 44945–54. doi:10.1074/jbc.M407986200. PMC 1201506. PMID 15322084.
^ Riddell DR, Christie G, Hussain I, Dingwall C (Aug 2001). "Compartmentalization of beta-secretase (Asp2) into low-buoyant density, noncaveolar lipid rafts". Current Biology 11 (16): 1288–93. doi:10.1016/S0960-9822(01)00394-3. PMID 11525745.
^ Selkoe D, Kopan R (2003). "Notch and Presenilin: regulated intramembrane proteolysis links development and degeneration". Annual Review of Neuroscience 26 (1): 565–97. doi:10.1146/annurev.neuro.26.041002.131334. PMID 12730322.
^ Phinney AL, Calhoun ME, Wolfer DP, Lipp HP, Zheng H, Jucker M (1999). "No hippocampal neuron or synaptic bouton loss in learning-impaired aged beta-amyloid precursor protein-null mice". Neuroscience 90 (4): 1207–16. doi:10.1016/S0306-4522(98)00645-9. PMID 10338291.
^ Matsuyama S, Teraoka R, Mori H, Tomiyama T (2007). "Inverse correlation between amyloid precursor protein and synaptic plasticity in transgenic mice". Neuroreport 18 (10): 1083–7. doi:10.1097/WNR.0b013e3281e72b18. PMID 17558301.
^ Barger SW, DeWall KM, Liu L, Mrak RE, Griffin WS (Aug 2008). "Relationships between expression of apolipoprotein E and beta-amyloid precursor protein are altered in proximity to Alzheimer beta-amyloid plaques: potential explanations from cell culture studies". Journal of Neuropathology and Experimental Neurology 67 (8): 773–83. doi:10.1097/NEN.0b013e318180ec47. PMC 3334532. PMID 18648325.
^ Satpute-Krishnan P, DeGiorgis JA, Conley MP, Jang M, Bearer EL (Oct 2006). "A peptide zipcode sufficient for anterograde transport within amyloid precursor protein". Proceedings of the National Academy of Sciences of the United States of America 103 (44): 16532–7. doi:10.1073/pnas.0607527103. PMC 1621108. PMID 17062754.
^ Seamster PE, Loewenberg M, Pascal J, Chauviere A, Gonzales A, Cristini V, Bearer EL (Oct 2012). "Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon". Physical Biology 9 (5): 055005. doi:10.1088/1478-3975/9/5/055005. PMC 3625656. PMID 23011729.
^ Rogers JT, Bush AI, Cho HH, Smith DH, Thomson AM, Friedlich AL, Lahiri DK, Leedman PJ, Huang X, Cahill CM (Dec 2008). "Iron and the translation of the amyloid precursor protein (APP) and ferritin mRNAs: riboregulation against neural oxidative damage in Alzheimer's disease". Biochemical Society Transactions 36 (Pt 6): 1282–7. doi:10.1042/BST0361282. PMC 2746665. PMID 19021541.
^ Ebrahimi KH, Hagedoorn PL, Hagen WR (2012). "A synthetic peptide with the putative iron binding motif of amyloid precursor protein (APP) does not catalytically oxidize iron". PLOS ONE 7 (8): e40287. doi:10.1371/journal.pone.0040287. PMC 3419245. PMID 22916096.
^ Honarmand Ebrahimi K, Dienemann C, Hoefgen S, Than ME, Hagedoorn PL, Hagen WR (2013). "The amyloid precursor protein (APP) does not have a ferroxidase site in its E2 domain". PLOS ONE 8 (8): e72177. doi:10.1371/journal.pone.0072177. PMID 23977245.
^ McCarthy RC, Park YH, Kosman DJ (Jul 2014). "sAPP modulates iron efflux from brain microvascular endothelial cells by stabilizing the ferrous iron exporter ferroportin". EMBO Reports 15 (7): 809–15. doi:10.15252/embr.201338064. PMC 4196985. PMID 24867889.
^ Porayette P, Gallego MJ, Kaltcheva MM, Meethal SV, Atwood CS (Dec 2007). "Amyloid-beta precursor protein expression and modulation in human embryonic stem cells: a novel role for human chorionic gonadotropin". Biochemical and Biophysical Research Communications 364 (3): 522–7. doi:10.1016/j.bbrc.2007.10.021. PMID 17959150.
^ Porayette P, Gallego MJ, Kaltcheva MM, Bowen RL, Vadakkadath Meethal S, Atwood CS (Aug 2009). "Differential processing of amyloid-beta precursor protein directs human embryonic stem cell proliferation and differentiation into neuronal precursor cells". The Journal of Biological Chemistry 284 (35): 23806–17. doi:10.1074/jbc.M109.026328. PMC 2749153. PMID 19542221.
^ Gallego MJ, Porayette P, Kaltcheva MM, Meethal SV, Atwood CS (Jun 2009). "Opioid and progesterone signaling is obligatory for early human embryogenesis". Stem Cells and Development 18 (5): 737–40. doi:10.1089/scd.2008.0190. PMC 2891507. PMID 18803462.
^ Gallego MJ, Porayette P, Kaltcheva MM, Bowen RL, Vadakkadath Meethal S, Atwood CS (2010). "The pregnancy hormones human chorionic gonadotropin and progesterone induce human embryonic stem cell proliferation and differentiation into neuroectodermal rosettes". Stem Cell Research & Therapy 1 (4): 28. doi:10.1186/scrt28. PMC 2983441. PMID 20836886.
^ McPhie DL, Coopersmith R, Hines-Peralta A, Chen Y, Ivins KJ, Manly SP, Kozlowski MR, Neve KA, Neve RL (Jul 2003). "DNA synthesis and neuronal apoptosis caused by familial Alzheimer disease mutants of the amyloid precursor protein are mediated by the p21 activated kinase PAK3". The Journal of Neuroscience 23 (17): 6914–27. PMID 12890786.
^ Bowen RL, Verdile G, Liu T, Parlow AF, Perry G, Smith MA, Martins RN, Atwood CS (May 2004). "Luteinizing hormone, a reproductive regulator that modulates the processing of amyloid-beta precursor protein and amyloid-beta deposition". The Journal of Biological Chemistry 279 (19): 20539–45. doi:10.1074/jbc.M311993200. PMID 14871891.
^ ^a ^b ^c Biederer T, Cao X, Südhof TC, Liu X (Sep 2002). "Regulation of APP-dependent transcription complexes by Mint/X11s: differential functions of Mint isoforms". The Journal of Neuroscience 22 (17): 7340–51. PMID 12196555.
^ ^a ^b Borg JP, Ooi J, Levy E, Margolis B (Nov 1996). "The phosphotyrosine interaction domains of X11 and FE65 bind to distinct sites on the YENPTY motif of amyloid precursor protein". Molecular and Cellular Biology 16 (11): 6229–41. doi:10.1128/mcb.16.11.6229. PMC 231626. PMID 8887653.
^ ^a ^b Araki Y, Tomita S, Yamaguchi H, Miyagi N, Sumioka A, Kirino Y, Suzuki T (Dec 2003). "Novel cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein-mediated stabilization of amyloid beta-protein precursor metabolism". The Journal of Biological Chemistry 278 (49): 49448–58. doi:10.1074/jbc.M306024200. PMID 12972431.
^ Tomita S, Ozaki T, Taru H, Oguchi S, Takeda S, Yagi Y, Sakiyama S, Kirino Y, Suzuki T (Jan 1999). "Interaction of a neuron-specific protein containing PDZ domains with Alzheimer's amyloid precursor protein". The Journal of Biological Chemistry 274 (4): 2243–54. doi:10.1074/jbc.274.4.2243. PMID 9890987.
^ Tanahashi H, Tabira T (Feb 1999). "X11L2, a new member of the X11 protein family, interacts with Alzheimer's beta-amyloid precursor protein". Biochemical and Biophysical Research Communications 255 (3): 663–7. doi:10.1006/bbrc.1999.0265. PMID 10049767.
^ Zambrano N, Buxbaum JD, Minopoli G, Fiore F, De Candia P, De Renzis S, Faraonio R, Sabo S, Cheetham J, Sudol M, Russo T (Mar 1997). "Interaction of the phosphotyrosine interaction/phosphotyrosine binding-related domains of Fe65 with wild-type and mutant Alzheimer's beta-amyloid precursor proteins". The Journal of Biological Chemistry 272 (10): 6399–405. doi:10.1074/jbc.272.10.6399. PMID 9045663.
^ Guénette SY, Chen J, Jondro PD, Tanzi RE (Oct 1996). "Association of a novel human FE65-like protein with the cytoplasmic domain of the beta-amyloid precursor protein". Proceedings of the National Academy of Sciences of the United States of America 93 (20): 10832–7. doi:10.1073/pnas.93.20.10832. PMC 38241. PMID 8855266.
^ Tanahashi H, Tabira T (Feb 1999). "Molecular cloning of human Fe65L2 and its interaction with the Alzheimer's beta-amyloid precursor protein". Neuroscience Letters 261 (3): 143–6. doi:10.1016/S0304-3940(98)00995-1. PMID 10081969.
^ Trommsdorff M, Borg JP, Margolis B, Herz J (Dec 1998). "Interaction of cytosolic adaptor proteins with neuronal apolipoprotein E receptors and the amyloid precursor protein". The Journal of Biological Chemistry 273 (50): 33556–60. doi:10.1074/jbc.273.50.33556. PMID 9837937.
^ Chow N, Korenberg JR, Chen XN, Neve RL (May 1996). "APP-BP1, a novel protein that binds to the carboxyl-terminal region of the amyloid precursor protein". The Journal of Biological Chemistry 271 (19): 11339–46. doi:10.1074/jbc.271.19.11339. PMID 8626687.
^ Zheng P, Eastman J, Vande Pol S, Pimplikar SW (Dec 1998). "PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein". Proceedings of the National Academy of Sciences of the United States of America 95 (25): 14745–50. doi:10.1073/pnas.95.25.14745. PMC 24520. PMID 9843960.
^ Wang B, Nguyen M, Breckenridge DG, Stojanovic M, Clemons PA, Kuppig S, Shore GC (Apr 2003). "Uncleaved BAP31 in association with A4 protein at the endoplasmic reticulum is an inhibitor of Fas-initiated release of cytochrome c from mitochondria". The Journal of Biological Chemistry 278 (16): 14461–8. doi:10.1074/jbc.M209684200. PMID 12529377.
^ Lefterov IM, Koldamova RP, Lazo JS (Sep 2000). "Human bleomycin hydrolase regulates the secretion of amyloid precursor protein". FASEB Journal 14 (12): 1837–47. doi:10.1096/fj.99-0938com. PMID 10973933.
^ Araki Y, Miyagi N, Kato N, Yoshida T, Wada S, Nishimura M, Komano H, Yamamoto T, De Strooper B, Yamamoto K, Suzuki T (Jun 2004). "Coordinated metabolism of Alcadein and amyloid beta-protein precursor regulates FE65-dependent gene transactivation". The Journal of Biological Chemistry 279 (23): 24343–54. doi:10.1074/jbc.M401925200. PMID 15037614.
^ Ikezu T, Trapp BD, Song KS, Schlegel A, Lisanti MP, Okamoto T (Apr 1998). "Caveolae, plasma membrane microdomains for alpha-secretase-mediated processing of the amyloid precursor protein". The Journal of Biological Chemistry 273 (17): 10485–95. doi:10.1074/jbc.273.17.10485. PMID 9553108.
^ Hashimoto T, Wakabayashi T, Watanabe A, Kowa H, Hosoda R, Nakamura A, Kanazawa I, Arai T, Takio K, Mann DM, Iwatsubo T (Apr 2002). "CLAC: a novel Alzheimer amyloid plaque component derived from a transmembrane precursor, CLAC-P/collagen type XXV". The EMBO Journal 21 (7): 1524–34. doi:10.1093/emboj/21.7.1524. PMC 125364. PMID 11927537.
^ Ohsawa I, Takamura C, Kohsaka S (Mar 2001). "Fibulin-1 binds the amino-terminal head of beta-amyloid precursor protein and modulates its physiological function". Journal of Neurochemistry 76 (5): 1411–20. doi:10.1046/j.1471-4159.2001.00144.x. PMID 11238726.
^ Chauhan VP, Ray I, Chauhan A, Wisniewski HM (May 1999). "Binding of gelsolin, a secretory protein, to amyloid beta-protein". Biochemical and Biophysical Research Communications 258 (2): 241–6. doi:10.1006/bbrc.1999.0623. PMID 10329371.
^ Yan SD, Fu J, Soto C, Chen X, Zhu H, Al-Mohanna F, Collison K, Zhu A, Stern E, Saido T, Tohyama M, Ogawa S, Roher A, Stern D (Oct 1997). "An intracellular protein that binds amyloid-beta peptide and mediates neurotoxicity in Alzheimer's disease". Nature 389 (6652): 689–95. doi:10.1038/39522. PMID 9338779.
^ Tarr PE, Roncarati R, Pelicci G, Pelicci PG, D'Adamio L (May 2002). "Tyrosine phosphorylation of the beta-amyloid precursor protein cytoplasmic tail promotes interaction with Shc". The Journal of Biological Chemistry 277 (19): 16798–804. doi:10.1074/jbc.M110286200. PMID 11877420.
^ Hoe HS, Lee KJ, Carney RS, Lee J, Markova A, Lee JY, Howell BW, Hyman BT, Pak DT, Bu G, Rebeck GW (Jun 2009). "Interaction of reelin with amyloid precursor protein promotes neurite outgrowth". The Journal of Neuroscience 29 (23): 7459–73. doi:10.1523/JNEUROSCI.4872-08.2009. PMC 2759694. PMID 19515914. Lay summary – Alzheimer Research Forum.

External links

GeneReviews/NCBI/NIH/UW entry on Early-Onset Familial Alzheimer Disease
Amyloid Protein Precursor at the US National Library of Medicine Medical Subject Headings (MeSH)
Entrez Gene: APP amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease)

UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

1. アミロイド疾患の遺伝的要因 genetic factors in the amyloid diseases
2. アルツハイマー病の遺伝学 genetics of alzheimer disease
3. 脳アミロイドアンギオパチー cerebral amyloid angiopathy
4. アミロイドーシスの概要 an overview of amyloidosis
5. 続発性（AA）アミロイドーシスの病因 pathogenesis of secondary aa amyloidosis

English Journal

Expression pattern of a single transgene cassette located in endogenous GLIS3 of cloned pigs; a nested situation.

Jakobsen JE, Dantoft TM, Johansen MG, Jørgensen AL.AbstractOne of the main focus areas in transgenesis is the choice of a promoter driving stable expression over time of the gene of interest. Besides promoter identity, the genomic environment of the transgene plays a pivotal role in transcription regulation. Studies in higher mammals describing transgene expression from a defined locus are very limited. We set out to determine the expression pattern of two transgene promoters, the human PDGFβ and the viral SV40, in a single cassette positioned in the largest intron of the porcine GLIS3 locus. The PDGFβ promoter drives a variant of the amyloid precursor protein gene named APP695sw and the SV40 promoter drives the neomycin resistant gene, Neo. The nested gene scenario was investigated in three transgenic cloned pigs sacrificed at 3months, 2years and 3years of age. With identical genetic make-up and same environment, the three individual pigs are considered representative of 3year lifespan of a single pig. Selected organs from the pigs were analyzed by quantitative RT-PCR for transgene promoter activity as well as endogenous GLIS3 promoter activity. No apparent effect of the transgene cassette was observed on endogenous GLIS3 expression. In addition, one year old homozygous pigs showed no phenotypic signs of dysfunctional GLIS3. Both transgene promoters showed and retained their tissue specificity with stable expression over time. Our study indicates that transgenes inserted in a nested situation might be applicable for faithful and long term transgene expression.
Gene.Gene.2012 Jul 10;502(2):94-8. Epub 2012 Apr 25.
One of the main focus areas in transgenesis is the choice of a promoter driving stable expression over time of the gene of interest. Besides promoter identity, the genomic environment of the transgene plays a pivotal role in transcription regulation. Studies in higher mammals describing transgene ex
PMID 22555020

Genetic testing in familial and young-onset Alzheimer's disease: mutation spectrum in a Serbian cohort.

Dobricic V, Stefanova E, Jankovic M, Gurunlian N, Novakovic I, Hardy J, Kostic V, Guerreiro R.SourceInstitute of Neurology, Clinical Center of Serbia, Belgrade, Serbia.
Neurobiology of aging.Neurobiol Aging.2012 Jul;33(7):1481.e7-1481.e12. Epub 2012 Jan 4.
Alzheimer's disease (AD) is the most common form of dementia. To date, more than 200 mutations in three genes have been identified as cause of early-onset autosomal dominant inherited AD. The aim of this study was to characterize the mutation spectrum and describe genotype-phenotype correlations in
PMID 22221884

Japanese Journal

Continuation of Exercise Is Necessary to Inhibit High Fat Diet-Induced β-Amyloid Deposition and Memory Deficit in Amyloid Precursor Protein Transgenic Mice.

Maesako Masato,Uemura Kengo,Iwata Ayana,Kubota Masakazu,Watanabe Kiwamu,Uemura Maiko,Noda Yasuha,Asada-Utsugi Megumi,Kihara Takeshi,Takahashi Ryosuke,Shimohama Shun,Kinoshita Ayae
PloS one 8(9), 2013-09-04
… It has been previously reported that HFD clearly worsens cognitive function in amyloid precursor protein (APP) transgenic mice. … On the other hand, we have demonstrated that voluntary exercise in an enriched environment is an effective intervention to rescue HFD-induced β-amyloid (Aβ) deposition and memory deficit. …
NAID 120005324901

Modeling Alzheimer's Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness

Kondo Takayuki,Asai Masashi,Tsukita Kayoko,Kutoku Yumiko,Ohsawa Yutaka,Sunada Yoshihide,Imamura Keiko,Egawa Naohiro,Yahata Naoki,Okita Keisuke,Takahashi Kazutoshi,Asaka Isao,Aoi Takashi,Watanabe Akira,Watanabe Kaori,Kadoya Chie,Nakano Rie,Watanabe Dai,Maruyama Kei,Hori Osamu,Hibino Satoshi,Choshi Tominari,Nakahata Tatsutoshi,Hioki Hiroyuki,Kaneko Takeshi,Naitoh Motoko,Yoshikawa Katsuhiro,Yamawaki Satoko,Suzuki Shigehiko,Hata Ryuji,Ueno Shu-ichi,Seki Tsuneyoshi,Kobayashi Kazuhiro,Toda Tatsushi,Murakami Kazuma,Irie Kazuhiro,Klein William L.,Mori Hiroshi,Asada Takashi,Takahashi Ryosuke,Iwata Nobuhisa,Yamanaka Shinya,Inoue Haruhisa
Cell Stem Cell 12(4), 487-496, 2013-04-04
… Oligomeric forms of amyloid-β peptide (Aβ) are thought to play a pivotal role in the pathogenesis of Alzheimer's disease (AD), but the mechanism involved is still unclear. … Aβ oligomers accumulated in iPSC-derived neurons and astrocytes in cells from patients with a familial amyloid precursor protein (APP)-E693Δ mutation and sporadic AD, leading to endoplasmic reticulum (ER) and oxidative stress. …
NAID 120005231417

Protective Effects of Ferulic Acid in Amyloid Precursor Protein Plus Presenilin-1 Transgenic Mouse Model of Alzheimer Disease

Yan Ji-Jing,Jung Jun-Sub,Kim Taek-Keun [他]
Biological & pharmaceutical bulletin 36(1), 140-143, 2013-01-00
NAID 40019536220

Related Pictures

★リンクテーブル★

リンク元	「アミロイド前駆体蛋白質」「APP」「アミロイド前駆体タンパク」
拡張検索	「amyloid precursor protein secretase」「amyloid precursor protein gene」「amyloid precursor proteins」
関連記事	「precursor」「precursor protein」「amyloid」

「アミロイド前駆体蛋白質」

Amyloid beta (A4) precursor protein
	PDB rendering based on 1aap
Available structures
PDB	Ortholog search: PDBe, RCSB
List of PDB id codes
	1AAP, 1AMB, 1AMC, 1AML, 1BA4, 1BA6, 1BJB, 1BJC, 1BRC, 1CA0, 1HZ3, 1IYT, 1MWP, 1OWT, 1QCM, 1QWP, 1QXC, 1QYT, 1TAW, 1TKN, 1UO7, 1UO8, 1UOA, 1UOI, 1X11, 1Z0Q, 1ZE7, 1ZE9, 1ZJD, 2BEG, 2BOM, 2BP4, 2FJZ, 2FK1, 2FK2, 2FK3, 2FKL, 2FMA, 2G47, 2IPU, 2LFM, 2LLM, 2LMN, 2LMO, 2LMP, 2LMQ, 2LNQ, 2LOH, 2LP1, 2LZ3, 2LZ4, 2M4J, 2M9R, 2M9S, 2MGT, 2MJ1, 2MPZ, 2MVX, 2MXU, 2OTK, 2R0W, 2WK3, 2Y29, 2Y2A, 2Y3J, 2Y3K, 2Y3L, 3AYU, 3BAE, 3BKJ, 3DXC, 3DXD, 3DXE, 3GCI, 3IFL, 3IFN, 3IFO, 3IFP, 3JQ5, 3JQL, 3JTI, 3KTM, 3L33, 3L81, 3MOQ, 3MXC, 3MXY, 3NYJ, 3NYL, 3OVJ, 3OW9, 3SV1, 3U0T, 3UMH, 3UMI, 3UMK, 4HIX, 4JFN, 4M1C, 4MDR, 4NGE, 4OJF, 4ONF, 4ONG, 4PQD, 4PWQ
Identifiers
Symbols	APP ; AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2
External IDs	OMIM: 104760 MGI: 88059 HomoloGene: 56379 ChEMBL: 2487 GeneCards: APP Gene
Gene ontology
Molecular function	• DNA binding; • serine-type endopeptidase inhibitor activity; • receptor binding; • protein binding; • heparin binding; • peptidase activator activity; • enzyme binding; • acetylcholine receptor binding; • identical protein binding; • transition metal ion binding; • PTB domain binding; • growth factor receptor binding;
Cellular component	• extracellular region; • extracellular space; • nuclear envelope lumen; • cytoplasm; • endosome; • smooth endoplasmic reticulum; • Golgi apparatus; • cytosol; • plasma membrane; • integral component of plasma membrane; • coated pit; • cell-cell junction; • cell surface; • integral component of membrane; • ER to Golgi transport vesicle; • axon; • platelet alpha granule lumen; • neuromuscular junction; • endosome lumen; • trans-Golgi network membrane; • ciliary rootlet; • terminal bouton; • dendritic spine; • dendritic shaft; • intracellular membrane-bounded organelle; • receptor complex; • membrane raft; • apical part of cell; • synapse; • perinuclear region of cytoplasm; • spindle midzone; • extracellular exosome;
Biological process	• suckling behavior; • platelet degranulation; • mRNA polyadenylation; • regulation of translation; • protein phosphorylation; • cellular copper ion homeostasis; • post-Golgi vesicle-mediated transport; • endocytosis; • response to oxidative stress; • cell adhesion; • regulation of epidermal growth factor-activated receptor activity; • Notch signaling pathway; • axonogenesis; • blood coagulation; • mating behavior; • locomotory behavior; • axon cargo transport; • cholesterol metabolic process; • adult locomotory behavior; • visual learning; • negative regulation of endopeptidase activity; • positive regulation of peptidase activity; • positive regulation of G2/M transition of mitotic cell cycle; • axon midline choice point recognition; • neuron remodeling; • dendrite development; • platelet activation; • extracellular matrix organization; • forebrain development; • neuron projection development; • ionotropic glutamate receptor signaling pathway; • nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway; • regulation of multicellular organism growth; • regulation of protein binding; • innate immune response; • negative regulation of neuron differentiation; • positive regulation of mitotic cell cycle; • positive regulation of transcription from RNA polymerase II promoter; • collateral sprouting in absence of injury; • regulation of synapse structure or activity; • neuromuscular process controlling balance; • synaptic growth at neuromuscular junction; • neuron apoptotic process; • smooth endoplasmic reticulum calcium ion homeostasis; • membrane organization;
	Sources: Amigo / QuickGO
RNA expression pattern
	More reference expression data
Orthologs
	v; t; e;

　　[★]

英: amyloid precursor protein, amyloid precursor proteins, APP
同: アミロイドβ蛋白質前駆体 amyloid β-protein precursor β-APP

[show details]

アミロイド前駆体蛋白質 : 約 36,300 件
アミロイド前駆体蛋白 : 約 32,100 件
アミロイド前駆蛋白質 : 約 8,680 件
アミロイド前駆蛋白 : 約 6,700 件

アミロイド前駆体タンパク質 : 約 68,500 件
アミロイド前駆体タンパク : 約 10,700 件
アミロイド前駆タンパク質 : 約 8,680 件
アミロイド前駆タンパク : 99 件