RNAリガーゼ
- 関
- tRNA ligase
WordNet
- the 18th letter of the Roman alphabet (同)r
PrepTutorEJDIC
- resistance / 17歳以下父兄同伴映画の表示 / rook
- ribonucleic acid・リボ核酸
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/04/13 22:51:45」(JST)
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RNA ligase (ATP) |
Identifiers |
EC number |
6.5.1.3 |
CAS number |
37353-39-2 |
Databases |
IntEnz |
IntEnz view |
BRENDA |
BRENDA entry |
ExPASy |
NiceZyme view |
KEGG |
KEGG entry |
MetaCyc |
metabolic pathway |
PRIAM |
profile |
PDB structures |
RCSB PDB PDBe PDBsum |
Gene Ontology |
AmiGO / EGO |
Search |
PMC |
articles |
PubMed |
articles |
NCBI |
proteins |
|
In enzymology, an RNA ligase (ATP) (EC 6.5.1.3) is an enzyme that catalyzes the chemical reaction
- ATP + (ribonucleotide)n + (ribonucleotide)m AMP + diphosphate + (ribonucleotide)n+m
The 3 substrates of this enzyme are ATP, (ribonucleotide)n, and (ribonucleotide)m, whereas its 3 products are AMP, diphosphate, and (ribonucleotide)n+m.
This enzyme belongs to the family of ligases, specifically those forming phosphoric-ester bonds. The systematic name of this enzyme class is poly(ribonucleotide):poly(ribonucleotide) ligase (AMP-forming). Other names in common use include polyribonucleotide synthase (ATP), RNA ligase, polyribonucleotide ligase, and ribonucleic ligase.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1VDX and 2C5U.
References
- Silber R, Malathi VG, Hurwitz J (1972). "Purification and properties of bacteriophage T4-induced RNA ligase". Proc. Natl. Acad. Sci. U.S.A. 69 (10): 3009–13. doi:10.1073/pnas.69.10.3009. PMC 389696. PMID 4342972.
UpToDate Contents
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English Journal
- Functional characterization of the human translocator protein (18kDa) gene promoter in human breast cancer cell lines.
- Batarseh A, Barlow KD, Martinez-Arguelles DB, Papadopoulos V.SourceThe Research Institute of the McGill University Health Centre and the Department of Medicine, McGill University, Montreal, Quebec, Canada H3G 1A4.
- Biochimica et biophysica acta.Biochim Biophys Acta.2012 Jan;1819(1):38-56. Epub 2011 Sep 18.
- The translocator protein (18kDa; TSPO) is a mitochondrial drug- and cholesterol-binding protein that has been implicated in several processes, including steroidogenesis, cell proliferation, and apoptosis. Expression of the human TSPO gene is elevated in several cancers. To understand the molecular m
- PMID 21958735
- Ubiquitin-mediated modulation of the cytoplasmic viral RNA sensor RIG-I.
- Oshiumi H, Matsumoto M, Seya T.SourceDepartment of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku Sapporo 060-8638, Japan.
- Journal of biochemistry.J Biochem.2012 Jan;151(1):5-11. Epub 2011 Sep 2.
- RIG-I-like receptors, including RIG-I, MDA5 and LGP2, recognize cytoplasmic viral RNA. The RIG-I protein consists of N-terminal CARDs, central RNA helicase and C-terminal domains. RIG-I activation is regulated by ubiquitination. Three ubiquitin ligases target the RIG-I protein. TRIM25 and Riplet ubi
- PMID 21890623
- Methods for small RNA preparation for digital gene expression profiling by next-generation sequencing.
- Linsen SE, Cuppen E.SourceHubrecht Institute, Utrecht, The Netherlands.
- Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2012;822:205-17.
- Digital gene expression (DGE) profiling techniques are playing an eminent role in the detection, localization, and differential expression quantification of many small RNA species, including microRNAs (1-3). Procedures in small RNA library preparation techniques typically include adapter ligation by
- PMID 22144201
Japanese Journal
- The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.
- Oshiumi Hiroyuki,Miyashita Moeko,Inoue Naokazu,Okabe Masaru,Matsumoto Misako,Seya Tsukasa
- Cell Host & Microbe 8(6), 496-509, 2010-12-16
- … RNA virus infection is recognized by the RIG-I-likereceptors RIG-I and MDA5, which induce antiviralresponses including the production of type I inter-ferons (IFNs) and proinflammatory cytokines. … Fibroblasts, macro-phages, and conventional dendritic cells from Riplet-deficient animals were defective for the production ofIFN and other cytokines in response to infection withseveral RNA viruses. …
- NAID 120002725554
- The transDSL Ligase Ribozyme Can Utilize Various Forms of Modules to Clamp Its Substrate and Enzyme Units
- ISHIKAWA Junya,ISOMOTO Nana,FUJITA Yuki,FURUTA Hiroyuki,IKAWA Yoshiya
- Bioscience, biotechnology, and biochemistry 74(4), 872-874, 2010-04-23
- … TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. …
- NAID 10027554302
- Nedd4-Interacting Protein 2, a Short Half-life Membrane Protein Degraded in Lysosomes, Negatively Controls Down-Regulation of Connexin43
- Ohzono Chiho,Etoh Sachise,Matsumoto Masaki,Nakayama Keiichi I,Hirota Yuko,Tanaka Yoshitaka,Fujita Hideaki
- Biological & Pharmaceutical Bulletin 33(6), 951-957, 2010
- … It has been postulated that NDFIP2 acts as an adaptor for the ubiquitylation of substrates with Nedd4 ubiquitin ligase. … Intriguingly, knockdown (KD) of NDFIP2 with small interfering RNA (siRNA) impaired both the formation and function of gap junctions. …
- NAID 130000247960
Related Links
- 二価カチオン Mg 2+ (至適濃度:20 mM) 受容体がDNAの場合はATP再成系に必要なMn 2+ の方がよい。 DMSO 10~20%(40% DMSOでも活性あり) pCpを用いたRNAの末端ラベルで10% DMSOは2~3倍の活性化効果がある DNA ...
- T4 RNA Ligaseは、ATPに依存して、1本鎖RNAまたはDNAの分子間および分子内の連結を行い、1本鎖RNAまたはDNAの5′-末端のリン酸基と3′-末端の水酸基を結合します(1,2)。同様に、1本鎖RNAに[5′-32P]nucleoside 3′,5′-bis(phosphate ...
Related Pictures
★リンクテーブル★
[★]
- 英
- RNA ligase
- 関
- tRNAリガーゼ
[★]
トリプトファンtRNAリガーゼ
- 関
- tryptophanyl-tRNA synthetase
[★]
アスパラギン酸tRNAリガーゼ
- 関
- aspartyl-tRNA synthetase
[★]
システインtRNAリガーゼ
- 関
- cysteinyl-tRNA synthetase
[★]
チロシンtRNAリガーゼ
- 関
- tyrosyl-tRNA synthetase
[★]
tRNAリガーゼ
- 関
- RNA ligase
[★]
[★]
- 同
- リボ核酸 ribonucleic acid RNA
- 関
- DNA
[★]
リガーゼ、連結酵素
- 関
- synthetase