同: SSCP

WordNet

used of flowers having usually only one row or whorl of petals; "single chrysanthemums resemble daisies and may have more than one row of petals"
a base hit on which the batter stops safely at first base (同)bingle
not divided among or brought to bear on more than one object or objective; "judging a contest with a single eye"; "a single devotion to duty"; "undivided affection"; "gained their exclusive attention" (同)undivided, exclusive
existing alone or consisting of one entity or part or aspect or individual; "upon the hill stood a single tower"; "had but a single thought which was to escape"; "a single survivor"; "a single serving"; "a single lens"; "a single thickness"
having uniform application; "a single legal code for all"
hit a single; "the batter singled to left field"
drive (a vessel) ashore
line consisting of a complex of fibers or filaments that are twisted together to form a thread or a rope or a cable
a pattern forming a unity within a larger structural whole; "he tried to pick up the strands of his former life"; "I could hear several melodic strands simultaneously"
a poetic term for a shore (as the area periodically covered and uncovered by the tides)
to make melodious sounds; "The nightingale was singing"
deliver by singing; "Sing Christmas carols"
produce tones with the voice; "She was singing while she was cooking"; "My brother sings very well"
a symmetrical arrangement of the parts of a thing
(chemistry) the existence of different kinds of crystal of the same chemical compound (同)pleomorphism
(biology) the existence of two or more forms of individuals within the same animal species (independent of sex differences)
(genetics) the genetic variation within a population that natural selection can operate on

PrepTutorEJDIC

《名詞の前にのみ用いて》『たった一つ』(『一人』)『の』,単独の;《否定文で》ただ一つ(一人)(もない) / 《名詞の前にのみ用いて》『一人用の』・『独身の』・《名詞の前にのみ用いて》個々の,別々の / 単一の,単式の;(花が)一重咲きの,単弁の / 《英》(切符が)片道の / 〈C〉一人,1個;独身者 / 〈C〉《話》(ホテルの部屋,船室,列車寝台などの)一人用 / 〈C〉(野球で)シングルヒット,単打 / 《複数形で》(テニスなどの)シングルスの試合,単試合 / 《英》片道切符 / 《複数形で》《話》1ドル(ポンド)札 / …‘を'1人(1個)だけ選び出す《+『out』+『名,』+『名』+『out』》 / (野球で)シングルヒットを打つ
〈船〉‘を'座礁させる / 《受動態で》〈人〉‘を'置き去りにする,困った状態におく / 〈船が〉座礁する / 〈人が〉途方に暮れる,立ち往生する / 岸,浜
こ撚(よ)り(何本かより合わせて糸・なわなどにするその一本) / (より合わせた)糸,ひも;(頭髪の)房 / (真珠・ビーズなど)ひもに通したもの
『歌う』;(…に)歌って聞かせる《+『to』(『for』)+『名』》;(楽器に合わせて)歌う《+『to』+『名』》・〈小鳥・虫などが〉『鳴く』,さえずる / 《文》(…を)詩(歌)にする,(詩(歌)を詠んで)賛美する《+『of』+『名』》・〈小川・風・弾丸・湯沸かし・耳などが〉歌うように鳴る・《米語》自白する;密告する・〈歌〉‘を'歌う・《副詞[句]を伴って》歌って…‘を'(…に)する・…‘を'唱える,詠唱する・《文》…‘を'詩(歌)にする,(詩(歌)に詠んで)賛美する・合唱の集い
〈C〉形態,構造;調和のとれた配置(配列) / 〈U〉(型・性格などべの)一至,適合,適応《+『to』+『名』》

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/03/19 10:55:45」(JST)

wiki en

Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows sequences to be distinguished by means of gel electrophoresis, which separates fragments according to their different conformations.^[1]

Physical background

A single nucleotide change in a particular sequence, as seen in a double-stranded DNA, cannot be distinguished by electrophoresis, because the physical properties of the double strands are almost identical for both alleles. After denaturation, single-stranded DNA undergoes a 3-dimensional folding and may assume a unique conformational state based on its DNA sequence. The difference in shape between two single-stranded DNA strands with different sequences can cause them to migrate differently on an electrophoresis gel, even though the number of nucleotides is the same, which is, in fact, an application of SSCP.

Applications in molecular biology

SSCP used to be a way to discover new DNA polymorphisms apart from DNA sequencing, but is now being supplanted by sequencing techniques on account of efficiency and accuracy.^[2] These days, SSCP is most applicable as a diagnostic tool in molecular biology. It can be used in genotyping to detect homozygous individuals of different allelic states, as well as heterozygous individuals that should each demonstrate distinct patterns in an electrophoresis experiment.^[3] SSCP is also widely used in virology to detect variations in different strains of a virus, the idea being that a particular virus particle present in both strains will have undergone changes due to mutation, and that these changes will cause the two particles to assume different conformations and, thus, be differentiable on an SSCP gel.^[4]

References

^ Masato Orita, Hiroyuki Iwahana, Hiroshi Knazawa, Kenshi Hayashi and Takato Sekiya Detection of the polymorphisms of human DNA by gelelectrophoresis as single-strand conformation polymorphisms. Proc. Natd.Acad. Sci. USA 86, 2766-2770, 1989.
^ Tahira, T.; Kukita, Y.; Higasa, K.; Okazaki, Y.; Yoshinaga, A.; Hayashi, K. (2009). "Estimation of SNP allele frequencies by SSCP analysis of pooled DNA.". Methods Mol Biol 578: 193–207. doi:10.1007/978-1-60327-411-1_12. PMID 19768595.
^ Michiei Oto, Satoshi Miyake and Yasuhito Yuasa. Optimization of Nonradioisotopic Single Strand Conformation Polymorphism Analysis with a Conventional Minislab Gel Electrophoresis Apparatus. Analytical Biochemistry, 213, 19-22, 1993.
^ Karen Sumire Kubo, R. M. Stuart, J. Freitas-Astúa1, R. Antonioli-Luizon, E. C. Locali-Fabris, H. D. Coletta-Filho1, M. A. Machado and E. W. Kitajima. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid gene. Archives of Virology, Official Journal of the Virology Division of the International Union of Microbiological Societies, 21 May 2009.

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English Journal

Effects of genetic variants for the calpastatin gene on calpastatin activity and meat tenderness in Hanwoo (Korean cattle).

Chung H, Davis M.SourceAnimal Genetic Improvement Division, National Institute of Animal Science, Seonghwan, Cheonan 333801, Republic of Korea; Bovine Functional Genomics Laboratory ARS/USDA, 10300 Baltimore, Beltsville, MD 20705, USA.
Meat science.Meat Sci.2012 Mar;90(3):711-4. Epub 2011 Nov 4.
This study was designed to investigate the effects of calpastatin genotypes determined by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) on calpastatin activity (CAC) and Warner-Bratzler Shear Force (WBS). Longissimus muscles were prepared from 379 Hanwoo bulls aged app
PMID 22119671

Improvement of RNA-SIP by pyrosequencing to identify putative 4-n-nonylphenol degraders in activated sludge.

Zemb O, Lee M, Gutierrez-Zamora ML, Hamelin J, Coupland K, Hazrin-Chong NH, Taleb I, Manefield M.SourceCentre for Marine BioInnovation (CMB), School of Biotechnology and Biomolecular Sciences (BABS), University of New South Wales (UNSW), Sydney 2052, Australia.
Water research.Water Res.2012 Mar 1;46(3):601-10. Epub 2011 Nov 23.
Nonylphenols (NP) have estrogenic potential because of their phenolic ring, but the organisms involved in the degradation of this alkylated phenol remain unidentified. Using 16S ribosomal RNA (rRNA)-based stable isotope probing (SIP) and a new method based on pyrosequencing, we identified the bacter
PMID 22154106

Japanese Journal

Single-Strand Conformation Polymorphism for Molecular Variability Studies of Six Viroid Species

ELLEUCH Amine,HAMDI Imen,BESSAIES Nabiha [他]
Bioscience, Biotechnology, and Biochemistry 77(1), 182-188, 2013-01-00
NAID 40019562014

Homogeneity and Synchronous Dynamics of Microbial Communities in Particulate Biofilms: from Major Populations to Minor Groups

Gévaudan Gaëlle,Hamelin Jérôme,Dabert Patrick,Godon Jean-Jacques,Bernet Nicolas
Microbes and Environments 27(2), 142-148, 2012
… The microbial community structure was evaluated by Capillary Electrophoresis-Single Strand Conformation Polymorphism (CE-SSCP) fingerprinting and showed extraordinary homogeneity between particles, even though transitory community structures were observed when reactor operating conditions were modified. …
NAID 130001390778

A p53 Gene Mutation in Malignant Fibrous Histiocytoma Associated with Bone Infarction

YAMAMOTO Yasuhiro,TAKAKUWA Yasunari,KURODA Makoto,NAKASHIMA Hiroatsu,WASHIMI Yuki,ISHIMURA Daisuke,YAMADA Harumoto
Tohoku journal of experimental medicine 225(3), 215-220, 2011-11-01
NAID 10030196266

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★リンクテーブル★

リンク元	「SSCP」「一本鎖DNA高次構造多型」
拡張検索	「PCR-single strand conformation polymorphism」
関連記事	「conformation」「single」「strand」「single strand」「sing」