primary culture

• 初代培養

関

primary-cultured

WordNet

1. of first rank or importance or value; direct and immediate rather than secondary; "primary goals"; "a primary effect"; "primary sources"; "a primary interest"
2. a preliminary election where delegates or nominees are chosen (同)primary_election
3. one of the main flight feathers projecting along the outer edge of a birds wing (同)primary feather, primary quill
4. (astronomy) a celestial body (especially a star) relative to other objects in orbit around it
5. not derived from or reducible to something else; basic; "a primary instinct"
6. grow in a special preparation; "the biologist grows microorganisms"
7. (biology) the growing of microorganisms in a nutrient medium (such as gelatin or agar); "the culture of cells in a Petri dish"
8. the raising of plants or animals; "the culture of oysters"
9. a particular society at a particular time and place; "early Mayan civilization" (同)civilization, civilisation
10. the attitudes and behavior that are characteristic of a particular social group or organization; "the developing drug culture"; "the reason that the agency is doomed to inaction has something to do with the FBI culture"
11. the tastes in art and manners that are favored by a social group

PrepTutorEJDIC

1. 『第一の』,『主要な』 / 『初期の』,『初等の』,初級の / 『根本的な』,基本的な,本来の,直接的な / 《名詞の前にのみ用いて》(回路・コイル・巻き・電流などが)一次の / 原色(三原色の一つ) / 《米》=primary election
2. 〈U〉『教養』;修養;教化 / 〈C〉〈U〉『文化』(主として精神面をさし,生活・習慣・物の考え方などを含む)Greek cultureギリシア文化 / 〈U〉(…の)『耕作』;『栽培』,養殖,培養《+『of』+『名』》
3. 教養のある,洗練された,上品な / 栽培(養殖)された

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/11/30 13:50:50」(JST)

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Cell culture in a special tissue culture dish

Epithelial cells in culture, stained for keratin (red) and DNA (green)

Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. Cell culture conditions can vary for each cell type, but artificial environments consist of a suitable vessel with substrate or medium that supplies the essential nutrients (amino acids,carbohydrates, vitamins, minerals), growth factors, hormones, and gases (CO2, O2), and regulates the physio-chemical environment (pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture).

In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses.

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.

History

The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body. In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture.

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures.

Concepts in mammalian cell culture

Isolation of cells

Cells can be isolated from tissues for ex vivo culture in several ways. Cells can be easily purified from blood; however, only the white cells are capable of growth in culture. Mononuclear cells can be released from soft tissues by enzymatic digestion with enzymes such as collagenase, trypsin, or pronase, which break down the extracellular matrix. Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. This method is known as explant culture.

Cells that are cultured directly from a subject are known as primary cells. With the exception of some derived from tumors, most primary cell cultures have limited lifespan.

An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. Numerous cell lines are well established as representative of particular cell types.

Maintaining cells in culture

For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit).

Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO₂ for mammalian cells) in a cell incubator. Culture conditions vary widely for each cell type, and variation of conditions for a particular cell type can result in different phenotypes.

A bottle of DMEM cell culture medium

Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. Recipes for growth media can vary in pH, glucose concentration, growth factors, and the presence of other nutrients. The growth factors used to supplement media are often derived from the serum of animal blood, such as fetal bovine serum (FBS), bovine calf serum, equine serum, and porcine serum. One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. Current practice is to minimize or eliminate the use of these ingredients wherever possible and use human platelet lysate (hPL). This eliminates the worry of cross-species contamination when using FBS with human cells. hPL has emerged as a safe and reliable alternative as a direct replacement for FBS or other animal serum. In addition, chemically defined media can be used to eliminate any serum trace (human or animal), but this cannot always be accomplished with different cell types. Alternative strategies involve sourcing the animal blood from countries with minimum BSE/TSE risk, such as The United States, Australia and New Zealand, and using purified nutrient concentrates derived from serum in place of whole animal serum for cell culture.

Plating density (number of cells per volume of culture medium) plays a critical role for some cell types. For example, a lower plating density makes granulosa cells exhibit estrogen production, while a higher plating density makes them appear as progesterone-producing theca lutein cells.

Cells can be grown either in suspension or adherent cultures. Some cells naturally live in suspension, without being attached to a surface, such as cells that exist in the bloodstream. There are also cell lines that have been modified to be able to survive in suspension cultures so they can be grown to a higher density than adherent conditions would allow. Adherent cells require a surface, such as tissue culture plastic or microcarrier, which may be coated with extracellular matrix (such as collagen and laminin) components to increase adhesion properties and provide other signals needed for growth and differentiation. Most cells derived from solid tissues are adherent. Another type of adherent culture is organotypic culture, which involves growing cells in a three-dimensional (3-D) environment as opposed to two-dimensional culture dishes. This 3D culture system is biochemically and physiologically more similar to in vivo tissue, but is technically challenging to maintain because of many factors (e.g. diffusion).

Components of cell culture media

Component	Function
Carbon source (glucose/glutamine)	Source of energy
Amino acid	Building blocks of protein
Vitamins	Promote cell survival and growth
Balanced salt solution	It is an isotonic mixture of ions to act as cofactors for enzymatic reactions, cell adhesion etc.
Phenol red dye	pH indicator. The color of phenol red changes from orange/red at pH 7-7.4 to yellow at acidic(lower) pH and purple at basic (higher) pH.
Bicarbonate /HEPES buffer	It is used to maintain a balanced pH in the media

Growth conditions

Parameter
Temperature	37°C
CO2	5%
Humidity	95%

Cell line cross-contamination

Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line. Problems with cell line cross-contamination have even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. Major cell line repositories, including the American Type Culture Collection (ATCC), the European Collection of Cell Cultures (ECACC) and the German Collection of Microorganisms and Cell Cultures (DSMZ), have received cell line submissions from researchers that were misidentified by them. Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions. ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.

To address this problem of cell line cross-contamination, researchers are encouraged to authenticate their cell lines at an early passage to establish the identity of the cell line. Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis.

One significant cell-line cross contaminant is the immortal HeLa cell line.

Other technical issues

As cells generally continue to divide in culture, they generally grow to fill the available area or volume. This can generate several issues:

• Nutrient depletion in the growth media
• Changes in pH of the growth media
• Accumulation of apoptotic/necrotic (dead) cells
• Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition.
• Cell-to-cell contact can stimulate cellular differentiation.
• Genetic and epigenetic alterations, with a natural selection of the altered cells potentially leading to overgrowth of abnormal, culture-adapted cells with decreased differentiation and increased proliferative capacity.

Manipulation of cultured cells

Among the common manipulations carried out on culture cells are media changes, passaging cells, and transfecting cells. These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid contamination with bacteria, yeast, or other cell lines. Manipulations are typically carried out in a biosafety hood or laminar flow cabinet to exclude contaminating micro-organisms. Antibiotics (e.g. penicillin and streptomycin) and antifungals (e.g.amphotericin B) can also be added to the growth media.

As cells undergo metabolic processes, acid is produced and the pH decreases. Often, a pH indicator is added to the medium to measure nutrient depletion.

Media changes

In the case of adherent cultures, the media can be removed directly by aspiration, and then is replaced. Media changes in non-adherent cultures involve centrifuging the culture and resuspending the cells in fresh media.

Passaging cells

Main article: Passaging

Passaging (also known as subculture or splitting cells) involves transferring a small number of cells into a new vessel. Cells can be cultured for a longer time if they are split regularly, as it avoids the senescence associated with prolonged high cell density. Suspension cultures are easily passaged with a small amount of culture containing a few cells diluted in a larger volume of fresh media. For adherent cultures, cells first need to be detached; this is commonly done with a mixture of trypsin-EDTA; however, other enzyme mixes are now available for this purpose. A small number of detached cells can then be used to seed a new culture. Some cell cultures, such as RAW cells are mechanically scraped from the surface of their vessel with rubber scrapers.

Transfection and transduction

Main articles: Transfection and Transformation (genetics)

Another common method for manipulating cells involves the introduction of foreign DNA by transfection. This is often performed to cause cells to express a gene of interest. More recently, the transfection of RNAi constructs have been realized as a convenient mechanism for suppressing the expression of a particular gene/protein. DNA can also be inserted into cells using viruses, in methods referred to as transduction, infection or transformation. Viruses, as parasitic agents, are well suited to introducing DNA into cells, as this is a part of their normal course of reproduction.

Established human cell lines

Cultured HeLa cells have been stained with Hoechst turning their nuclei blue, and are one of the earliest human cell lines descended from Henrietta Lacks, who died of cervical cancer from which these cells originated.

Cell lines that originate with humans have been somewhat controversial in bioethics, as they may outlive their parent organism and later be used in the discovery of lucrative medical treatments. In the pioneering decision in this area, the Supreme Court of California held in Moore v. Regents of the University of California that human patients have no property rights in cell lines derived from organs removed with their consent.

For more details on this topic, see Hybridoma.

It is possible to fuse normal cells with an immortalised cell line. This method is used to produce monoclonal antibodies. In brief, lymphocytes isolated from the spleen (or possibly blood) of an immunised animal are combined with an immortal myeloma cell line (B cell lineage) to produce a hybridoma which has the antibody specificity of the primary lymphocyte and the immortality of the myeloma. Selective growth medium (HA or HAT) is used to select against unfused myeloma cells; primary lymphoctyes die quickly in culture and only the fused cells survive. These are screened for production of the required antibody, generally in pools to start with and then after single cloning.

Cell strains

A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or characteristics which must be defined. Cell strains are cells that have been adapted to culture but, unlike cell lines, have a finite division potential. Non-immortalized cells stop dividing after 40 to 60 population doublings and, after this, they lose their ability to proliferate (a genetically determined event known as senescence).

Applications of cell culture

Mass culture of animal cell lines is fundamental to the manufacture of viral vaccines and other products of biotechnology. Culture of human stem cells is used to expand the number of cells and differentiate the cells into various somatic cell types for transplantation. Stem cell culture is also used to harvest the molecules and exosomes that the stem cells release for the purposes of therapeutic development.

Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes, synthetic hormones, immunobiologicals (monoclonal antibodies, interleukins, lymphokines), and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures, more complex proteins that are glycosylated (carbohydrate-modified) currently must be made in animal cells. An important example of such a complex protein is the hormone erythropoietin. The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants, use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene expression and confocal microscopy observation is one of its applications. It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the process.

Cell culture is also a key technique for cellular agriculture, which aims to provide both new products and new ways of producing existing agricultural products like milk, (cultured) meat, fragrances, and rhino horn from cells and microorganisms. It is therefore considered one means of achieving animal-free agriculture.

Cell culture in two dimensions

Research in tissue engineering, stem cells and molecular biology primarily involves cultures of cells on flat plastic dishes. This technique is known as two-dimensional (2D) cell culture, and was first developed by Wilhelm Roux who, in 1885, removed a portion of the medullary plate of an embryonic chicken and maintained it in warm saline for several days on a flat glass plate. From the advance of polymer technology arose today's standard plastic dish for 2D cell culture, commonly known as the Petri dish. Julius Richard Petri, a German bacteriologist, is generally credited with this invention while working as an assistant to Robert Koch. Various researchers today also utilize culturing laboratory flasks, conicals, and even disposable bags like those used in single-use bioreactors.

Aside from Petri dishes, scientists have long been growing cells within biologically derived matrices such as collagen or fibrin, and more recently, on synthetic hydrogels such as polyacrylamide or PEG. They do this in order to elicit phenotypes that are not expressed on conventionally rigid substrates. There is growing interest in controlling matrix stiffness, a concept that has led to discoveries in fields such as:

• Stem cell self-renewal
• Lineage specification
• Cancer cell phenotype
• Fibrosis
• Hepatocyte function
• Mechanosensing

Cell culture in three dimensions

Cell culture in three dimensions has been touted as "Biology's New Dimension". At present, the practice of cell culture remains based on varying combinations of single or multiple cell structures in 2D. Currently, there is an increase in use of 3D cell cultures in research areas including drug discovery, cancer biology, regenerative medicine and basic life science research. There are a variety of platforms used to facilitate the growth of three-dimensional cellular structures including scaffold systems such as hydrogel matrices and solid scaffolds, and scaffold-free systems such as low-adhesion plates, nanoparticle facilitated magnetic levitation, and hanging drop plates.

3D cell culture in hydrogels

As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of cells, different hydrogel culture matrices mimicking natural ECM structure are seen as potential approaches to in vivo –like cell culturing. Hydrogels are composed of interconnected pores with high water retention, which enables efficient transport of substances such as nutrients and gases. Several different types of hydrogels from natural and synthetic materials are available for 3D cell culture, including animal ECM extract hydrogels, protein hydrogels, peptide hydrogels, polymer hydrogels, and wood-based nanocellulose hydrogel.

3D Cell Culturing by Magnetic Levitation

The 3D Cell Culturing by Magnetic Levitation method (MLM) is the application of growing 3D tissue by inducing cells treated with magnetic nanoparticle assemblies in spatially varying magnetic fields using neodymium magnetic drivers and promoting cell to cell interactions by levitating the cells up to the air/liquid interface of a standard petri dish. The magnetic nanoparticle assemblies consist of magnetic iron oxide nanoparticles, gold nanoparticles, and the polymer polylysine. 3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high-throughput low volume systems.

Tissue culture and engineering

Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers dramatic improvements in low cost medical care for hundreds of thousands of patients annually.

Vaccines

Vaccines for polio, measles, mumps, rubella, and chickenpox are currently made in cell cultures. Due to the H5N1 pandemic threat, research into using cell culture for influenza vaccines is being funded by the United States government. Novel ideas in the field include recombinant DNA-based vaccines, such as one made using human adenovirus (a common cold virus) as a vector, and novel adjuvants.

Culture of non-mammalian cells

Plant cell culture methods

Main article: Plant tissue culture

See also: Tobacco BY-2 cells

Plant cell cultures are typically grown as cell suspension cultures in a liquid medium or as callus cultures on a solid medium. The culturing of undifferentiated plant cells and calli requires the proper balance of the plant growth hormones auxin and cytokinin.

Insect cell culture

Cells derived from Drosophila melanogaster (most prominently, Schneider 2 cells) can be used for experiments which may be hard to do on live flies or larvae, such as biochemical studies or studies using siRNA. Cell lines derived from the army worm Spodoptera frugiperda, including Sf9 and Sf21, and from the cabbage looper Trichoplusia ni, High Five cells, are commonly used for expression of recombinant proteins using baculovirus.

Bacterial and yeast culture methods

Main article: Microbiological culture

For bacteria and yeasts, small quantities of cells are usually grown on a solid support that contains nutrients embedded in it, usually a gel such as agar, while large-scale cultures are grown with the cells suspended in a nutrient broth.

Viral culture methods

Main article: Viral culture

The culture of viruses requires the culture of cells of mammalian, plant, fungal or bacterial origin as hosts for the growth and replication of the virus. Whole wild type viruses, recombinant viruses or viral products may be generated in cell types other than their natural hosts under the right conditions. Depending on the species of the virus, infection and viral replication may result in host cell lysis and formation of a viral plaque.

Common cell lines

Human cell lines

• DU145 (prostate cancer)
• H295R (adrenocortical cancer)
• HeLa (cervical cancer)
• KBM-7 (chronic myelogenous leukemia)
• LNCaP (prostate cancer)
• MCF-7 (breast cancer)
• MDA-MB-468 (breast cancer)
• PC3 (prostate cancer)
• SaOS-2 (bone cancer)
• SH-SY5Y (neuroblastoma, cloned from a myeloma)
• T47D (breast cancer)
• THP-1 (acute myeloid leukemia)
• U87 (glioblastoma)
• National Cancer Institute's 60 cancer cell line panel (NCI60)

Primate cell lines

• Vero (African green monkey Chlorocebus kidney epithelial cell line)

Mouse cell lines

• MC3T3 (embryonic calvarium)

Rat tumor cell lines

• GH3 (pituitary tumor)
• PC12 (pheochromocytoma)

Plant cell lines

• Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell)

Other species cell lines

• Dog MDCK kidney epithelial
• Xenopus A6 kidney epithelial
• Zebrafish AB9

List of cell lines

This list is incomplete; you can help by expanding it.

Cell line	Meaning	Organism	Origin tissue	Morphology	Links
3T3-L1	"3-day transfer, inoculum 3 x 10^5 cells"	Mouse	Embryo	Fibroblast	ECACC Cellosaurus
4T1		Mouse	Mammary gland		ATCC Cellosaurus
9L		Rat	Brain	Glioblastoma	ECACC Cellosaurus
A172		Human	Brain	Glioblastoma	ECACC Cellosaurus
A20		Mouse	B lymphoma	B lymphocyte	Cellosaurus
A253		Human	Submandibular duct	Head and neck carcinoma	ATCC Cellosaurus
A2780		Human	Ovary	Ovarian carcinoma	ECACC Cellosaurus
A2780ADR		Human	Ovary	Adriamycin-resistant derivative of A2780	ECACC Cellosaurus
A2780cis		Human	Ovary	Cisplatin-resistant derivative of A2780	ECACC Cellosaurus
A431		Human	Skin epithelium	Squamous cell carcinoma	ECACC Cellosaurus
A549		Human	Lung	Lung carcinoma	ECACC Cellosaurus
AB9		Zebrafish	Fin	Fibroblast	ATCC Cellosaurus
AHL-1	Armenian Hamster Lung-1	Hamster	Lung		ECACC Cellosaurus
ALC		Mouse	Bone marrow	Stroma	PMID 2435412 Cellosaurus
B16		Mouse	Melanoma		ECACC Cellosaurus
B35		Rat	Neuroblastoma		ATCC Cellosaurus
BCP-1		Human	PBMC	HIV+ primary effusion lymphoma	ATCC Cellosaurus
BEAS-2B	Bronchial epithelium + Adenovirus 12-SV40 virus hybrid (Ad12SV40)	Human	Lung	Epithelial	ECACC Cellosaurus
bEnd.3	Brain Endothelial 3	Mouse	Brain/cerebral cortex	Endothelium	Cellosaurus
BHK-21	Baby Hamster Kidney-21	Hamster	Kidney	Fibroblast	ECACC Cellosaurus
BOSC23	Packaging cell line derived from HEK 293	Human	Kidney (embryonic)	Epithelium	Cellosaurus
BT-20	Breast Tumor-20	Human	Breast epithelium	Breast carcinoma	ATCC Cellosaurus
BxPC3	Biopsy xenograft of Pancreatic Carcinoma line 3	Human	Pancreatic adenocarcinoma	Epithelial	ECACC Cellosaurus
C2C12		Mouse	Myoblast		ECACC Cellosaurus
C3H-10T1/2		Mouse	Embryonic mesenchymal cell line		ECACC Cellosaurus
C6		Rat	Brain astrocyte	Glioma	ECACC Cellosaurus
C6/36		Insect - Asian tiger mosquito	Larval tissue		ECACC Cellosaurus
Caco-2		Human	Colon	Colorectal carcinoma	ECACC Cellosaurus
Cal-27		Human	Tongue	Squamous cell carcinoma	ATCC Cellosaurus
CGR8		Mouse	Embryonic stem cells		ECACC Cellosaurus
CHO	Chinese Hamster Ovary	Hamster	Ovary	Epithelium	ECACC Cellosaurus
CML T1	Chronic myeloid leukemia T lymphocyte 1	Human	CML acute phase	T cell leukemia	DSMZ Cellosaurus
CMT12	Canine Mammary Tumor 12	Dog	Mammary gland	Epithelium	Cellosaurus
COR-L23		Human	Lung	Lung carcinoma	ECACC Cellosaurus
COR-L23/5010		Human	Lung	Lung carcinoma	ECACC Cellosaurus
COR-L23/CPR		Human	Lung	Lung carcinoma	ECACC Cellosaurus
COR-L23/R23-		Human	Lung	Lung carcinoma	ECACC Cellosaurus
COS-7	Cercopithecus aethiops, origin-defective SV-40	Old World monkey - Cercopithecus aethiops (Chlorocebus)	Kidney	Fibroblast	ECACC Cellosaurus
COV-434		Human	Ovary	Ovarian granulosa cell carcinoma	PMID 8436435 ECACC Cellosaurus
CT26		Mouse	Colon	Colorectal carcinoma	Cellosaurus
D17		Dog	Lung metastasis	Osteosarcoma	ATCC Cellosaurus
DH82		Dog	Histiocytosis	Monocyte/macrophage	ECACC Cellosaurus
DU145		Human	Androgen insensitive prostate carcinoma		ATCC Cellosaurus
DuCaP	Dura mater cancer of the Prostate	Human	Metastatic prostate carcinoma	Epithelial	PMID 11317521 Cellosaurus
E14Tg2a		Mouse		Embryonic stem cells	ECACC Cellosaurus
EL4		Mouse		T cell leukemia	ECACC Cellosaurus
EM-2		Human	CML blast crisis	Ph+ CML line	DSMZ Cellosaurus
EM-3		Human	CML blast crisis	Ph+ CML line	DSMZ Cellosaurus
EMT6/AR1		Mouse	Mammary gland	Epithelial-like	ECACC Cellosaurus
EMT6/AR10.0		Mouse	Mammary gland	Epithelial-like	ECACC Cellosaurus
FM3		Human	Lymph node metastasis	Melanoma	ECACC Cellosaurus
GL261	Glioma 261	Mouse	Brain	Glioma	Cellosaurus
H1299		Human	Lung	Lung carcinoma	ATCC Cellosaurus
HaCaT		Human	Skin	Keratinocyte	CLS Cellosaurus
HCA2		Human	Colon	Adenocarcinoma	ECACC Cellosaurus
HEK 293	Human Embryonic Kidney 293	Human	Kidney (embryonic)	Epithelium	ECACC Cellosaurus
HEK 293T	HEK 293 derivative	Human	Kidney (embryonic)	Epithelium	ECACC Cellosaurus
HeLa	"Henrietta Lacks"	Human	Cervix epithelium	Cervical carcinoma	ECACC Cellosaurus
Hepa1c1c7	Clone 7 of clone 1 hepatoma line 1	Mouse	Hepatoma	Epithelial	ECACC Cellosaurus
Hep G2		Human	Liver	Hepatoblastoma	ECACC Cellosaurus
High Five		Insect (moth) - Trichoplusia ni	Ovary		Cellosaurus
HL-60	Human Leukemia-60	Human	Blood	Myeloblast	ECACC Cellosaurus
HT-1080		Human		Fibrosarcoma	ECACC Cellosaurus
HT-29		Human	Colon epithelium	Adenocarcinoma	ECACC Cellosaurus
J558L		Mouse	Myeloma	B lymphocyte cell	ECACC Cellosaurus
Jurkat		Human	White blood cells	T cell leukemia	ECACC Cellosaurus
JY		Human	Lymphoblastoid	EBV-transformed B cell	ECACC Cellosaurus
K562		Human	Lymphoblastoid	CML blast crisis	ECACC Cellosaurus
KBM-7		Human	Lymphoblastoid	CML blast crisis	Cellosaurus
KCL-22		Human	Lymphoblastoid	CML	DSMZ Cellosaurus
KG1		Human	Lymphoblastoid	AML	ECACC Cellosaurus
Ku812		Human	Lymphoblastoid	Erythroleukemia	ECACC Cellosaurus
KYO-1	Kyoto-1	Human	Lymphoblastoid	CML	DSMZ Cellosaurus
L1210		Mouse	Lymphocytic leukemia	Ascitic fluid	ECACC Cellosaurus
L243		Mouse	Hybridoma	Secretes L243 mAb (against HLA-DR)	ATCC Cellosaurus
LNCaP	Lymph Node Cancer of the Prostate	Human	Prostatic adenocarcinoma	Epithelial	ECACC Cellosaurus
MA2.1		Mouse	Hybridoma	Secretes MA2.1 mAb (against HLA-A2 and HLA-B17)	ATCC Cellosaurus
Ma-Mel 1, 2, 3....48		Human	Skin	A range of melanoma cell lines	ECACC Cellosaurus
MC-38	Mouse Colon-38	Mouse	Colon	Adenocarcinoma	Cellosaurus
MCF-7	Michigan Cancer Foundation-7	Human	Breast	Invasive breast ductal carcinoma ER+, PR+	ECACC Cellosaurus
MCF-10A	Michigan Cancer Foundation-10A	Human	Breast epithelium		ATCC Cellosaurus
MDA-MB-157	M.D. Anderson - Metastatic Breast-157	Human	Pleural effusion metastasis	Breast carcinoma	ECACC Cellosaurus
MDA-MB-231	M.D. Anderson - Metastatic Breast-231	Human	Pleural effusion metastasis	Breast carcinoma	ECACC Cellosaurus
MDA-MB-361	M.D. Anderson - Metastatic Breast-361	Human		Melanoma (contaminated by M14)	ECACC Cellosaurus
MDA-MB-468	M.D. Anderson - Metastatic Breast-468	Human	Pleural effusion metastasis	Breast carcinoma	ATCC Cellosaurus
MDCK II	Madin Darby Canine Kidney II	Dog	Kidney	Epithelium	ECACC Cellosaurus
MG63		Human	Bone	Osteosarcoma	ECACC Cellosaurus
MOR/0.2R		Human	Lung	Lung carcinoma	ECACC Cellosaurus
Mono-Mac-6		Human	White blood cells	Myeloid metaplasic AML	DSMZ Cellosaurus
MRC-5	Medical Research Council cell strain 5	Human	Lung (fetal)	Fibroblast	ECACC Cellosaurus
MTD-1A		Mouse		Epithelium	Cellosaurus
MyEnd	Myocardial Endothelial	Mouse		Endothelium	Cellosaurus
NCI-H69		Human	Lung	Lung carcinoma	ECACC Cellosaurus
NCI-H69/CPR		Human	Lung	Lung carcinoma	ECACC Cellosaurus
NCI-H69/LX10		Human	Lung	Lung carcinoma	ECACC Cellosaurus
NCI-H69/LX20		Human	Lung	Lung carcinoma	ECACC Cellosaurus
NCI-H69/LX4		Human	Lung	Lung carcinoma	ECACC Cellosaurus
Neuro-2a		Mouse	Nerve/neuroblastoma	Neuronal stem cells	ECACC Cellosaurus
NIH-3T3	NIH, 3-day transfer, inoculum 3 x 105 cells	Mouse	Embryo	Fibroblast	ECACC Cellosaurus
NALM-1		Human	Peripheral blood	Blast-crisis CML	ATCC Cellosaurus
Neuro2a		Mouse	Nerve/neuroblastoma	Neuronal stem cells	Cellosaurus
NK-92		Human	Leukemia/lymphoma		ATCC Cellosaurus
NTERA-2		Human	Lung metastasis	Embryonal carcinoma	ECACC Cellosaurus
NW-145		Human	Skin	Melanoma	ESTDAB Cellosaurus
OK	Opossum Kidney	Virginia opossum - Didelphis virginiana	Kidney		ECACC Cellosaurus
OPCN / OPCT cell lines		Human	Prostate	Range of prostate tumour lines	Cellosaurus
P3X63Ag8		Mouse	Myeloma		ECACC Cellosaurus
PC12		Rat	Adrenal medulla	Pheochromocytoma	ECACC Cellosaurus
PC-3	Prostate Cancer-3	Human	Bone metastasis	Prostate carcinoma	ECACC Cellosaurus
Peer		Human	T cell leukemia		DSMZ Cellosaurus
PNT1A		Human	Prostate	SV40-transformed tumour line	ECACC Cellosaurus
PNT2		Human	Prostate	SV40-transformed tumour line	ECACC Cellosaurus
Pt K2	The second cell line derived from Potorous tridactylis	Long-nosed potoroo - Potorous tridactylus	Kidney	Epithelial	ECACC Cellosaurus
Raji		Human	B lymphoma	Lymphoblast-like	ECACC Cellosaurus
RBL-1	Rat Basophilic Leukemia-1	Rat	Leukemia	Basophil cell	ECACC Cellosaurus
RenCa	Renal Carcinoma	Mouse	Kidney	Renal carcinoma	ATCC Cellosaurus
RIN-5F		Mouse	Pancreas		ECACC Cellosaurus
RMA-S		Mouse		T cell tumour	Cellosaurus
S2	Schneider 2	Insect - Drosophila melanogaster	Late stage (20–24 hours old) embryos		ATCC Cellosaurus
SaOS-2	Sarcoma OSteogenic-2	Human	Bone	Osteosarcoma	ECACC Cellosaurus
Sf21	Spodoptera frugiperda 21	Insect (moth) - Spodoptera frugiperda	Ovary		ECACC Cellosaurus
Sf9	Spodoptera frugiperda 9	Insect (moth) - Spodoptera frugiperda	Ovary		ECACC Cellosaurus
SH-SY5Y		Human	Bone marrow metastasis	Neuroblastoma	ECACC Cellosaurus
SiHa		Human	Cervix epithelium	Cervical carcinoma	ATCC Cellosaurus
SK-BR-3	Sloan-Kettering Breast cancer 3	Human	Breast	Breast carcinoma	DSMZ Cellosaurus
SK-OV-3	Sloan-Kettering Ovarian cancer 3	Human	Ovary	Ovarian carcinoma	ECACC Cellosaurus
T2		Human		T cell leukemia/B cell line hybridoma	ATCC Cellosaurus
T-47D		Human	Breast	Breast ductal carcinoma	ECACC Cellosaurus
T84		Human	Lung metastasis	Colorectal carcinoma	ECACC Cellosaurus
T98G		Human	Glioblastoma-astrocytoma	Epithelium	ECACC Cellosaurus
THP-1		Human	Monocyte	Acute monocytic leukemia	ECACC Cellosaurus
U373		Human	Glioblastoma-astrocytoma	Epithelium	ECACC Cellosaurus
U87		Human	Glioblastoma-astrocytoma	Epithelial-like	ECACC Cellosaurus
U937		Human	Leukemic monocytic lymphoma		ECACC Cellosaurus
VCaP	Vertebral Cancer of the Prostate	Human	Vertebra metastasis	Prostate carcinoma	ECACC Cellosaurus
Vero	Vero (truth)	African green monkey - Chlorocebus sabaeus	Kidney epithelium		ECACC Cellosaurus
VG-1		Human		Primary effusion lymphoma	Cellosaurus
WM39		Human	Skin	Melanoma	ESTDAB Cellosaurus
WT-49		Human	Lymphoblastoid		ECACC Cellosaurus
YAC-1		Mouse	Lymphoma		ECACC Cellosaurus
YAR		Human	Lymphoblastoid	EBV-transformed B cell	Human Immunology ECACC Cellosaurus

See also

• Biological immortality
• Cell culture assays
• Electric cell-substrate impedance sensing
• List of contaminated cell lines

References and notes

• Public Health England, Public Health England Culture Collections (ECACC)
• MacLeod, R. A. F.; Dirks, Wilhelm G.; Matsuo, Yoshinobu; Kaufmann, Maren; Milch, Herbert; Drexler, Hans G. (1999). "Widespread intraspecies cross-contamination of human tumour cell lines". International Journal of Cancer. 83 (4): 555–563. doi:10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2. PMID 10508494. 
• Masters, John R. (2002). "HeLa cells 50 years on: the good, the bad and the ugly". Nature Reviews Cancer. 2 (4): 315–319. doi:10.1038/nrc775. PMID 12001993. 
• Recently invented was the 3D Petri dish, the first 3D cell culture offering.

External links

Library resources about Cell culture

Resources in your library Resources in other libraries

• Table of common cell lines from Alberts 4th ed.
• Cancer Cells in Culture
• Evolution of Cell Culture Surfaces
• Hypertext version of the Cell Line Data Base
• Microcarrier Cell Culture Handbook by GE Healthcare Life Sciences
• Cell Culture Applications - Resources including application notes and protocols to create an ideal environment for growing cells, right from the start.
• Cell Culture Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique including basic cell culture protocols and video training
• Database of Who's Who in Cell Culture and Related Research
• Witkowski, Jan A. (2012). "Experimental pathology and the origins of tissue culture: Leo Loeb's contribution". Medical History. 27 (3): 269–88. doi:10.1017/S0025727300042964. PMC 1139336. PMID 6353093. 
• Coriell Cell Repositories
• Strategies for Protein Purification Handbook
• An Introduction To Cell Culture. This webinar introduces the history, theory, basic techniques, and potential pit-falls of mammalian cell culture.
• The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc.
• Pacey, Laura; Stead, Shelley; Gleave, Jacqueline; Tomczyk, Kasia; Doering, Laurie (2006). "Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation". Protocol Exchange. doi:10.1038/nprot.2006.215. 
• Gilabert, Juan A.; Montalvo, Gema B.; Artalejo, Antonio R. (2006). "Rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays". Protocol Exchange. doi:10.1038/nprot.2006.294. 

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Related Pictures

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★リンクテーブル★

リンク元	「初代培養」
拡張検索	「primary cultured neuron」「primary-cultured」「primary cultured cell」
関連記事	「primary」「culture」

「初代培養」

　　[★]

英

primary culture、primary-cultured

「primary cultured neuron」

　　[★]

• 初代培養神経細胞、初代培養ニューロン

「primary-cultured」

　　[★]

• 初代培養した

関

primary culture

「primary cultured cell」

　　[★]

• 初代・養細胞、初代細胞

「primary」

　　[★]

• adj.

• 一次の、第一次の、主要な、(化学)第一級の、一級の、初生の、(病名)原発性の、原発の、プライマリーの

関

cardinal、chief、first-order、foremost、main、mainly、major、master、primarily、principal、principally

　 　 　 　 　 　

「culture」

　　[★]

• n.

• 文化、培地