挿入配列
WordNet
- determine the order of constituents in; "They sequenced the human genome"
- a following of one thing after another in time; "the doctor saw a sequence of patients" (同)chronological sequence, succession, successiveness, chronological succession
- film consisting of a succession of related shots that develop a given subject in a movie (同)episode
- serial arrangement in which things follow in logical order or a recurrent pattern; "the sequence of names was alphabetical"; "he invented a technique to determine the sequence of base pairs in DNA"
- several repetitions of a melodic phrase in different keys
- arrange in a sequence
- the act of putting one thing into another (同)introduction, intromission
PrepTutorEJDIC
- 〈U〉〈C〉(時間の上の,また因果関係のつながりによる)『連続』,続き / 〈C〉《a~》(…の)一連のもの《+『of』+『名』》 / 〈U〉(起こる)『順序』(order),筋道 / 〈C〉(…に対する)結果《+『to』+『名』》
- 〈U〉挿入,差し込み / 〈C〉挿入物;(新物の)折り込み広告;(語句の)書き加え;(レース刺繍(ししゅう)の)縫いつけ,はめ込み
- 配列,接続;(特に時間の)調整
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2016/10/31 05:16:47」(JST)
[Wiki en表示]
An insertion sequence (also known as an IS, an insertion sequence element, or an IS element) is a short DNA sequence that acts as a simple transposable element. Insertion sequences have two major characteristics: they are small relative to other transposable elements (generally around 700 to 2500 bp in length) and only code for proteins implicated in the transposition activity (they are thus different from other transposons, which also carry accessory genes such as antibiotic resistance genes). These proteins are usually the transposase which catalyses the enzymatic reaction allowing the IS to move, and also one regulatory protein which either stimulates or inhibits the transposition activity. The coding region in an insertion sequence is usually flanked by inverted repeats. For example, the well-known IS911 (1250 bp) is flanked by two 36bp inverted repeat extremities and the coding region has two genes partially overlapping orfA and orfAB, coding the transposase (OrfAB) and a regulatory protein (OrfA). [1] A particular insertion sequence may be named according to the form ISn, where n is a number (e.g. IS1, IS2, IS3, IS10, IS50, IS911, IS26 etc.); this is not the only naming scheme used, however. Although insertion sequences are usually discussed in the context of prokaryotic genomes, certain eukaryotic DNA sequences belonging to the family of Tc1/mariner transposable elements may be considered to be, insertion sequences. [2]
Diagram illustrating the role of insertion sequences ("IS") in a composite transposon
In addition to occurring autonomously, insertion sequences may also occur as parts of composite transposons; in a composite transposon, two insertion sequences flank one or more accessory genes, such as an antibiotic resistance gene (e.g. Tn10, Tn5). Nevertheless, there exist another sort of transposons, called unit transposons, that do not carry insertion sequences at their extremities (e.g. Tn7).
A complex transposon does not rely on flanking insertion sequences for resolvase. The resolvase is part of the tns genome and cuts at flanking inverted repeats.
See also
References
- Campbell, Neil A. and Reece, Jane B. (2002). Biology (6th ed.), pp. 345–346. San Francisco: Benjamin Cummings. ISBN 0-8053-6624-5.
- Mahillon, Jacques and Chandler, Michael (1998). "Insertion sequences". Microbiology and Molecular Biology Reviews 62 (3), 725-774. Retrieved January 11, 2006.
- Prescott, Lansing M.; Harley, John P.; and Klein, Donald A. (2002). Microbiology (5th ed.), pp. 298–299. New York: McGraw-Hill. ISBN 0-07-232041-9.
- Shuler, Michael L. and Kargi, Fikret (2002). Bioprocess Engineering: Basic Concepts (2nd ed.), p. 220. Upper Saddle River, NJ: Prentice Hall PTR. ISBN 0-13-081908-5.
External links
UpToDate Contents
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English Journal
- CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining.
- Bachu R1, Bergareche I1, Chasin LA2.
- Biotechnology and bioengineering.Biotechnol Bioeng.2015 Oct;112(10):2154-62. doi: 10.1002/bit.25629. Epub 2015 Jul 7.
- Mammalian cells are widely used for the production of therapeutic recombinant proteins, as these cells facilitate accurate folding and post-translational modifications often essential for optimum activity. Targeted insertion of a plasmid harboring a gene of interest into the genome of mammalian cell
- PMID 25943095
- Detection and quantification of bovine signal joint T-cell receptor excision circles.
- Hisazumi R1, Kayumi M2, Kikukawa R3, Nasu T4, Yasuda M5.
- Veterinary immunology and immunopathology.Vet Immunol Immunopathol.2015 Sep 15;167(1-2):86-90. doi: 10.1016/j.vetimm.2015.06.010. Epub 2015 Jun 25.
- A signal joint (sj) T-cell receptor excision circle (TREC) is produced by T-cell receptor (TCR) gene rearrangements during αβ T-cell maturation in the thymus. sjTREC have been studied as a marker of thymic function in several spices. We designed specific primers for δrec-ψJα sj region to identi
- PMID 26143006
- A hydrolase-based reporter system to uncover the protein splicing performance of an archaeal intein.
- von der Heyde A1, Lockhauserbäumer J, Uetrecht C, Elleuche S.
- Applied microbiology and biotechnology.Appl Microbiol Biotechnol.2015 Sep;99(18):7613-24. doi: 10.1007/s00253-015-6689-8. Epub 2015 May 31.
- Extein amino acid residues around the splice site junctions affect the functionality of inteins. To identify an optimal sequence context for efficient protein splicing of an intein from the thermoacidophilic archaeon Picrophilus torridus, single extein amino acid residues at the splice site junction
- PMID 26026939
Japanese Journal
- 音節継続時間を利用した直線検出に基づく音声検索語検出
- 大野哲平 ,秋葉 友良
- 情報処理学会論文誌 54(2), 484-494, 2013-02-15
- 情報通信網の発展とデータ記録コストの低減により,音声を含むマルチメディアコンテンツが増大している.現在主流となっているマルチメディアデータに対する検索システムが検索の根拠としているファイル名やタグ情報等の人手によるメタデータ付与は,人的コストが非常に高い.そこで,音声データから求めたい情報になるべく早く,低コストでアクセスできる検索技術が求められている.音声検索語検出(Spoken Term De …
- NAID 110009537043
- Optimization of Gas-Switching Sequence for InGaAs/GaAsP Superlattice Structures Using In situ Wafer Curvature Monitoring
- Ma ShaoJun,Sodabanlu Hassanet,Watanabe Kentaroh [他],Sugiyama Masakazu,Nakano Yoshiaki
- Jpn J Appl Phys 51(10), 10ND09-10ND09-3, 2012-10-25
- … By high-accuracy in situ curvature measurement during the growth of InGaAs/GaAsP superlattice structures by metal organic vapor phase epitaxy, we have successfully observed the effect of thin GaAs insertion layers between InGaAs wells and GaAsP barriers on strain control. … By analyzing curvature transients, we found that an inadequate gas-switching sequence induces the carry over of indium from the InGaAs layer to the overlying GaAs insertion layer. …
- NAID 40019456575
- Effects of endogenous proteins and microRNA target sequence in a positive feedback system.
- Kanda Genki N,Togashi Ryohei,Harashima Hideyoshi,Kamiya Hiroyuki
- Biological & pharmaceutical bulletin 35(9), 1534-1538, 2012-09-00
- … This hypothesis was examined by the following three strategies, designed to avoid the putative immunoreactions: (i) use of the endogenous secreted alkaline phosphatase (SEAP) protein as a reporter, (ii) replacement of vp16 with endogenous transcription factors, and (iii) insertion of the target sequence of microRNA expressed in cells of hematopoietic origin, to suppress GAL4-vp16 expression in antigen-presenting cells. …
- NAID 120005228269
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- Sources of Bacterial Insertion Sequences Bacterial insertion sequences were initially identified during studies of model genetic systems by their capacity to generate mutations as a result of their translocation. Interest ...
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挿入配列 insertion sequence
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- 英
- insertion sequence, IS
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- 関
- a sequence of、arrange、arrangement、array、barrage、consecutive、consequence、constellation、continually、continue、continuous、order、ordinal、outcome、output、product、result、resultant、sequencing、sequential、serial、series、thread
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- 関
- sequence