DNA合成

WordNet

the process of producing a chemical compound (usually by the union of simpler chemical compounds)
the combination of ideas into a complex whole (同)synthetic thinking
the 4th letter of the Roman alphabet (同)d

PrepTutorEJDIC

〈U〉総合,統合[すること] / 〈C〉総合体,統合されたもの / 〈U〉合成
deuteriumの化学記号
deoxyribonucleic acidディオキシリボ核酸

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/07/31 19:44:32」(JST)

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DNA synthesis is the natural or artificial creation of deoxyribonucleic acid (DNA) molecules. The term DNA synthesis can refer to any of the following in various contexts:

DNA replication - DNA biosynthesis (in vivo DNA amplification)
Polymerase chain reaction - enzymatic DNA synthesis (in vitro DNA amplification)
Gene synthesis - physically creating artificial gene sequences

DNA replication

In nature, such molecules are created by all living cells through the process of DNA replication, with replication initiator proteins splitting the existing DNA of the cell and making a copy of each split strand, with the copied strands then being joined together with their template strand into a new DNA molecule. Various means also exist to artificially stimulate the replication of naturally occurring DNA, or to create artificial gene sequences.

Polymerase chain reaction

A polymerase chain reaction is a form of enzymatic DNA synthesis in the laboratory, using cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA.

Gene synthesis

Artificial gene synthesis is the process of synthesizing a gene in vitro without the need for initial template DNA samples. In 2010 J. Craig Venter and his team were the first to use entirely synthesized DNA to create a self-replicating microbe, dubbed Mycoplasma laboratorium.^[1]

Oligonucleotide synthesis

Oligonucleotide synthesis is the chemical synthesis of sequences of nucleic acids. The process has been fully automated since the late 1970s and can be used to form desired genetic sequences as well as for other uses in medicine and molecular biology.

Base pair synthesis

Recent research has demonstrated the possibility of creating new nucleobase pairs in addition to the naturally occurring pairs, A-T (adenine - thymine) and G-C (guanine - cytosine). A third base pair could dramatically expand the number of amino acids that can be encoded by DNA, from the existing 20 amino acids to a theoretically possible 172.^[1]

References

^ ^a ^b Fikes, Bradley J. (May 8, 2014). "Life engineered with expanded genetic code". San Diego Union Tribune. Retrieved 8 May 2014.

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English Journal

Facile electrochemical biosensor based on a new bifunctional probe for label-free detection of CGG trinucleotide repeat.

He H, Xia J, Peng X, Chang G, Zhang X, Wang Y, Nakatani K, Lou Z, Wang S.SourceCollege of Chemistry and Chemical Engineer, Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecule, Hubei University, Youyi Road 368, Wuchang, Wuhan, Hubei 430062, PR China.
Biosensors & bioelectronics.Biosens Bioelectron.2013 Nov 15;49:282-9. doi: 10.1016/j.bios.2013.05.022. Epub 2013 May 28.
We have developed a simple and label-free electrochemical assay to detect CGG trinucleotide repeat. For this purpose, a new bifunctional probe (FecNCD2) was developed, in which a recognition part (naphthyridine carbamate dimmer, NCD) was connected with an electro-active part (ferrocenyl group) using
PMID 23774165

Facile synthesis of hierarchically aloe-like gold micro/nanostructures for ultrasensitive DNA recognition.

Shi L, Chu Z, Liu Y, Jin W, Chen X.SourceState Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
Biosensors & bioelectronics.Biosens Bioelectron.2013 Nov 15;49:184-91. doi: 10.1016/j.bios.2013.05.012. Epub 2013 May 14.
Well-defined hierarchically aloe-like gold micro/nanostructures (HAG) are one-step electrochemically fabricated without introducing any template or surfactant. The formation kinetics of the HAG can be described as a nucleation and three-dimensional growth process controlled by the reactant diffusion
PMID 23747359

Dendrimer-encapsulated copper as a novel oligonucleotides label for sensitive electrochemical stripping detection of DNA hybridization.

Gao H, Jiang X, Dong YJ, Tang WX, Hou C, Zhu NN.SourceDepartment of Chemistry, College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China.
Biosensors & bioelectronics.Biosens Bioelectron.2013 Oct 15;48:210-5. doi: 10.1016/j.bios.2013.04.021. Epub 2013 Apr 28.
This paper describes the synthesis and characterization of a novel electrochemical label for sensitive electrochemical stripping detection of DNA hybridization based on dendrimer-encapsulated copper. The generation 4.5 (G 4.5) carboxyl-terminated poly(amidoamine) dendrimer with a trimesyl core was u
PMID 23685561

Japanese Journal

リン原子修飾オリゴ核酸の立体選択的合成

有機合成化学協会誌 74(1), 35-48, 2016-01
NAID 40020709429

Signal Transduction Mechanism for Serotonin 5-HT2B Receptor-Mediated DNA Synthesis and Proliferation in Primary Cultures of Adult Rat Hepatocytes

Biological & pharmaceutical bulletin 39(1), 121-129, 2016-01
NAID 40020690803

Serotonin 5-HT2B Receptor-Stimulated DNA Synthesis and Proliferation Are Mediated by Autocrine Secretion of Transforming Growth Factor-α in Primary Cultures of Adult Rat Hepatocytes