CO2インキュベーター

WordNet

the 3rd letter of the Roman alphabet (同)c
(music) the keynote of the scale of C major
a general-purpose programing language closely associated with the UNIX operating system
apparatus consisting of a box designed to maintain a constant temperature by the use of a thermostat; used for chicks or premature infants (同)brooder

PrepTutorEJDIC

carbonの化学記号
ふ卵器,早産児保育器;細菌培養器
Colorado

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/09/10 21:25:45」(JST)

wiki en

[Wiki en表示]

A Bacteriological incubator

Interior of a CO₂ incubator used in cell culture

In biology, an incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO₂) and oxygen content of the atmosphere inside. Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial as well as eukaryotic cells.

Incubators are also used in the poultry industry to act as a substitute for hens. This often results in higher hatch rates due to the ability to control both temperature and humidity. Various brands of incubators are commercially available to breeders.

The simplest incubators are insulated boxes with an adjustable heater, typically going up to 60 to 65 °C (140 to 150 °F), though some can go slightly higher (generally to no more than 100 °C). The most commonly used temperature both for bacteria such as the frequently used E. coli as well as for mammalian cells is approximately 37 °C, as these organisms grow well under such conditions. For other organisms used in biological experiments, such as the budding yeast Saccharomyces cerevisiae, a growth temperature of 30 °C is optimal.

More elaborate incubators can also include the ability to lower the temperature (via refrigeration), or the ability to control humidity or CO₂ levels. This is important in the cultivation of mammalian cells, where the relative humidity is typically >80% to prevent evaporation and a slightly acidic pH is achieved by maintaining a CO₂ level of 5%.

Louis Pasteur used the small opening underneath his staircase as an incubator.

References

UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

1. 一酸化炭素拡散能 diffusing capacity for carbon monoxide
2. 一酸化炭素中毒 carbon monoxide poisoning
3. 肺症状を伴うHIV感染患者に対するアプローチ approach to the hiv infected patient with pulmonary symptoms
4. 高圧酸素療法 hyperbaric oxygen therapy
5. HIV陽性患者における腎臓病の概要 overview of kidney disease in hiv positive patients

English Journal

Successful "in-flight" activation of natural killer cells during long-distance shipping.

Koepsell SA, Kadidlo DM, Fautsch S, McCullough J, Klingemann H, Wagner JE, Miller JS, McKenna DH Jr.SourceDepartment of Laboratory Medicine and Pathology, Division of Transfusion Medicine, University of Minnesota Medical School, St. Paul, USA.
Transfusion.Transfusion.2013 Feb;53(2):398-403. doi: 10.1111/j.1537-2995.2012.03695.x. Epub 2012 May 11.
BACKGROUND: Natural killer (NK) cells have shown promise in the treatment of malignancy. However, the widespread use of these cells may be limited by both the lack of resources and the expertise needed to manufacture them and the apparent need to use only fresh cells. The NHLBI-sponsored Production
PMID 22574659

Neuroprotective effects of SNX-185 in an In Vitro model of TBI with a second insult.

Shahlaie K, Gurkoff GG, Lyeth BG, Muizelaar JP, Berman RF.SourceDepartment of Neurological Surgery, University of California, Davis, CA, USA.
Restorative neurology and neuroscience.Restor Neurol Neurosci.2013 Jan 1;31(2):141-53. doi: 10.3233/RNN-120243.
Purpose: Second insults following traumatic brain injury (TBI), such as ischemia and hypoxia, significantly worsen outcome in patients and in experimental models of TBI. Following TBI there is a pathological increase in intracellular calcium, triggering cellular mechanisms of dysfunction and death.
PMID 23247061

[The optimization of method for lentiviral vector to transfect CD34+ hematopoietic stem cells from human cord].

Zhou RQ, Gong YP, Lin J, He Q, Wan YF.SourceDepartment of Hematology and Research Lab of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition.Sichuan Da Xue Xue Bao Yi Xue Ban.2013 Jan;44(1):130-4.
OBJECTIVE: To identify the best transfect conditions for lentiviral vector to transfect CD34+ stem cells from human cord blood.METHODS: CD34+ hematopoietic stem cells from human cord blood were transduced with pTRIPdU3-RNAiTALh-EF1a-GFP plasmid expressing GFP by the second generation and third gener
PMID 23600227

Japanese Journal

リアルタイム細胞分析システムによる破骨細胞定量法を用いたエピガロカテキンガレートの細胞分化抑制効果

江守永,龍家圭,小口勝司 [他],岩井信市,天野均
昭和学士会雑誌 74(1), 73-80, 2014
… 細胞分化に対するEGCGの抑制効果の定量を試みた．雄性ddyマウス（5～8週齢）の大腿骨および脛骨より，骨髄細胞を採取した．骨髄細胞を100,000/wellで播種させた．EGCGを1µM，10µM，100µMの濃度で培地に添加させ，CO2インキュベーターで培養し，骨髄細胞を破骨細胞へと誘導した．計188時間の破骨細胞培養を行い，24時間後および72時間後に培地の交換およびEGCGの添加を行った．酒石酸抵抗性酸性ホスファターゼ（TRAP） …
NAID 130004692145

An Attempt of Cryopreservation of Mouse Embryos at the ACTREC Laboratory Animal Facility in India

THORAT Rahul,INGLE Arvind
Experimental animals 61(2), 139-145, 2012-04-01
… Out of 2,650 viable embryos, 2,359 embryos (89%) developed to the blastocyst stage after 24 h of incubation in a CO2 incubator. …
NAID 10030555892

A Self-Contained Culture Platform Using Carbon Dioxide Produced from a Chemical Reaction Supports Mouse Blastocyst Development In Vitro

Swain Jason E.
The Journal of reproduction and development 57(4), 551-555, 2011-08-01
… Elevated CO2 is required for in vitro embryo culture to maintain proper media pH and to supply embryo metabolic pathways. … As an alternative to current approaches using gas cylinders, we examined use of a chemical reaction to supply CO2. … A closed culture system was constructed and chemicals added to generate CO2, which was then supplied to developing embryos. …
NAID 10029593540