クリオピリン周期熱症候群
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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2018/01/15 00:05:57」(JST)
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"CAPS" redirects here. For other uses, see Caps (disambiguation).
The cleaved amplified polymorphic sequence or CAPS method is a technique in molecular biology for the analysis of genetic markers. It is an extension to the Restriction Fragment Length Polymorphism (RFLP) method, using polymerase chain reaction (PCR) to more quickly analyse the results.
Like RFLP, CAPS works on the principle that genetic differences between individuals can create or abolish restriction endonuclease restriction sites, and that these differences can be detected in the resulting DNA fragment length after digestion.
In the CAPS method, PCR amplification is directed across the altered restriction site, and the products digested with the restriction enzyme. When fractionated by agarose or acrylamide gel electrophoresis, the digested PCR products will give readily distinguishable patterns of bands. Alternatively, the amplified segment can be analyzed by Allele specific oligonucleotide (ASO) probes, a process that can often be done by a simple dot blot.
See also
References
External links
- https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechCAPS.shtml
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English Journal
- Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration.
- Meskinfam M1, Bertoldi S2, Albanese N3, Cerri A3, Tanzi MC4, Imani R5, Baheiraei N6, Farokhi M7, Farè S8.
- Materials science & engineering. C, Materials for biological applications.Mater Sci Eng C Mater Biol Appl.2018 Jan 1;82:130-140. doi: 10.1016/j.msec.2017.08.064. Epub 2017 Aug 17.
- PMID 29025641
- Uncovering the strategies of green development in a Chinese province driven by reallocating the emission caps of multiple pollutants among industries.
- Guo Y1, Zeng Z1, Tian J2, Xu F3, Chen L4, Zhou A5.
- The Science of the total environment.Sci Total Environ.2017 Dec 31;607-608:1487-1496. doi: 10.1016/j.scitotenv.2017.06.234. Epub 2017 Aug 5.
- PMID 28787800
- Application of RT-PCR and MALDI-TOF MS for the detection of RNA luteovirus.
- Kajiwara H1, Murakami R2.
- Analytical biochemistry.Anal Biochem.2017 Dec 15;539:45-47. doi: 10.1016/j.ab.2017.10.003. Epub 2017 Oct 6.
- PMID 28993140
Japanese Journal
- 臨床研究 一般臨床で行う色覚異常程度分類試案 : Farnsworth Dichotomous Test D-15のキャップ数と彩度のシミュレーション
- Identification and Parentage Analysis of Citrus Cultivars Developed in Japan by CAPS Markers
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