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1. ヘモグロビン分析法およびヘモグロビン異常症を特定するための検査法methods for hemoglobin analysis and hemoglobinopathy testing [show details]
… RBCs at different liquid densities based on hemoglobin density . A capillary tube is filled with solutions of different densities (two liquids in the SCD-AMPS-2… their net charge within a pH gradient on a gel medium (eg, polyacrylamide, thin-layer agarose). Hbs migrate through a pH gradient to a point at which their net charge is zero (ie,… and isoelectric focusing (IEF) have become the first-line methods of choice.…
2. 鎌状赤血球障害の診断diagnosis of sickle cell disorders [show details]
… Thin-layer isoelectric focusing is a highly accurate and cost effective tool for the diagnosis of sickle or other hemoglobin variants . The bands on isoelectric focusing are sharper… diagnosis of SCD are being developed, including paper-based tests that quantify sickle hemoglobin, a density-based rapid test using aqueous multiphase systems, and lateral flow immunoassays . These techniques …
3. α-1アンチトリプシン欠乏症の臨床症状、診断、および自然経過clinical manifestations diagnosis and natural history of alpha 1 antitrypsin deficiency [show details]
… Isoelectric focusing – Isoelectric focusing is the gold standard blood test for identifying AAT variant proteins, which migrate differently under isoelectric focusing.… have been used to predict mortality in patients with AAT-deficiency . As an example, decreased lung density as assessed by chest computed tomographic (CT) scan was associated with increased mortality in a …
4. リポ蛋白糸球体症lipoprotein glomerulopathy [show details]
… high cholesterol levels are observed in very low-density lipoprotein (VLDL) and intermediate density lipoprotein (IDL) fractions .… They differ based upon their migration properties as determined by isoelectric focusing polyacrylamide gel electrophoresis (IEF).…
5. 巨大気腫性嚢胞の評価および内科的マネージメントevaluation and medical management of giant bullae [show details]
…in combination with confirmation of a severely deficient genotype, generally determined by isoelectric focusing or genotyping. Arterial blood gases are usually only necessary in patients with severe … extruding from the wall of the bulla, a nodule or mass confined within the lumen, a soft-tissue density thickening the wall of the bulla, a pneumothorax, an air-fluid level, and hemorrhage . For patients …

「gradient gel isoelectric focusing」

「等電点」

英: isoelectric point pI

分子全体の電荷の総和が0となるpHを指す
該当するpHはpIで表す

	塩基性タンパク質	酸性タンパク質
優勢なアミノ酸	塩基性アミノ酸	酸性アミノ酸
想定される側鎖の末端	NH2	COOH
等電点(pI)	<7.4 酸性側	>7.4 アルカリ性側
pH7.4での電荷	-	+
代表的な蛋白質	アルブミン	ヒストン