gradient gel isoelectric focusing

密度勾配等電点電気泳動法

WordNet

1. a graded change in the magnitude of some physical quantity or dimension
2. the property possessed by a line or surface that departs from the horizontal; "a five-degree gradient" (同)slope
3. a colloid in a more solid form than a sol (同)colloidal gel
4. become a gel; "The solid, when heated, gelled"
5. cut off the testicles (of male animals such as horses); "the vet gelded the young horse" (同)cut
6. a unit of force equal to the force exerted by gravity; used to indicate the force to which a body is subjected when it is accelerated (同)gee, g-force

PrepTutorEJDIC

1. (道路・鉄道などの)勾配(こうばい),傾斜度,グラジエント・斜面
2. ゲル(コロイド溶液の凝固した状態;ゼラチン・寒天など) / =jell
3. 〈動物,特に馬〉‘を'去勢する

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• 1. 異常ヘモグロビン症の検査室診断 laboratory diagnosis of the hemoglobinopathies
• 2. デュシェンヌ型およびベッカー型筋ジストロフィーの臨床的特徴および診断 clinical features and diagnosis of duchenne and becker muscular dystrophy
• 3. 鎌状赤血球障害の診断 diagnosis of sickle cell disorders
• 4. α-1アンチトリプシン欠乏症の臨床症状、診断、および自然経過 clinical manifestations diagnosis and natural history of alpha 1 antitrypsin deficiency
• 5. ガラクトース血症：臨床的特徴および診断 galactosemia clinical features and diagnosis

English Journal

• A tunable isoelectric focusing via moving reaction boundary for two-dimensional gel electrophoresis and proteomics.

• Guo CG1, Shang Z1, Yan J1, Li S1, Li GQ1, Liu RZ2, Qing Y3, Fan LY1, Xiao H4, Cao CX5.
• Talanta.Talanta.2015 May;137:197-203. doi: 10.1016/j.talanta.2015.01.038. Epub 2015 Feb 3.
• Routine native immobilized pH gradient isoelectric focusing (IPG-IEF) and two-dimensional gel electrophoresis (2DE) are still suffering from unfortunate reproducibility, poor resolution (caused by protein precipitation) and instability in characterization of intact protein isoforms and posttranslati
• PMID 25770625

• In-gel peptide IEF sample preparation for LC/MS analysis.

• Berkelman T1, Bandhakavi S, Paulus A.
• Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2015;1295:369-79. doi: 10.1007/978-1-4939-2550-6_26.
• The technique of proteolytically digesting a sample and identifying its protein components by liquid chromatography followed by mass spectrometry (LC-MS) is a widely used analytical tool. Prior fractionation by isoelectric focusing (IEF) may be performed to increase the depth of proteome coverage. H
• PMID 25820734

• OFFGEL fractionation of peptides: where really is your sample?

• Franchin C1, Pivato M2, Rattazzi M3, Arrigoni G4, Millioni R5.
• Journal of chromatography. A.J Chromatogr A.2014 Aug 15;1355:278-83. doi: 10.1016/j.chroma.2014.05.077. Epub 2014 Jun 5.
• Shotgun proteomics of complex samples is generally coupled with at least one peptide fractionation step and, to this effect, peptide isoelectric focusing (IEF) in immobilized pH gradient (IPG) is one of the most used techniques. Fractionation with the OFFGEL 3100 Agilent Technologies apparatus allow
• PMID 24939089

Japanese Journal

• 全自動二次元電気泳動装置（Auto2D）における狭小幅イモビラインpH 勾配ゲルの評価

• 電気泳動 59(1), 9-12, 2015
• NAID 130005077626

• 一次元目にアガロースゲル等電点電気泳動を用いた蛍光ディファレンスゲル二次元電気泳動

• 生物物理化学 50(3Special), 173-178, 2006
• NAID 130003607378

• ゲノミクス・プロテオミクスを中心に‐ｏｍｉｃｓへの電気泳動法の応用　　プロテオミクス

• 生物物理化学 50(2), 33-36, 2006
• NAID 130003607367

Related Links

• Isoelectric Focusing | National Diagnostics

Isoelectric focusing employs a pH gradient extending the length of an electrophoresis gel. A protein stops migrating when it enters the zone in which the surrounding pH equals its isoelectric point, pI. At any other point in the gradient ...

• Isoelectric focusing - AES Electrophoresis Society

AES Application Focus Isoelectric Focusing Page 4 electrolyte strips. IPG strips for 2-D PAGE are often run with the gel facing down in dedicated IEF cells. Protocol 1. Casting gels for isoelectric focusing Equipment and ...

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★リンクテーブル★

リンク元	「密度勾配等電点電気泳動法」
関連記事	「isoelectric focusing」「gradient」「isoelectric」「g」

「密度勾配等電点電気泳動法」

　　[★]

英

gradient gel isoelectric focusing

関

等電点電気泳動法

「isoelectric focusing」

　　[★]

• 等電点電気泳動、等電点分離法

関

chromatofocusing、electrofocusing、IEF

同

IEF＆antibody heterogeneity

「gradient」

　　[★]

• 勾配、グラジエント

関

ramp、slope

　 　 　

「isoelectric」

　　[★]

• 等電点の、等電の

関

isoelectric point、pI

「g」

　　[★] グラム

関

gram