heteroduplex DNA

• ヘテロ二本鎖DNA、ヘテロ二重鎖DNA

関

nucleic acid heteroduplex

WordNet

1. the 4th letter of the Roman alphabet (同)d

PrepTutorEJDIC

1. deuteriumの化学記号
2. deoxyribonucleic acidディオキシリボ核酸

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/10/08 07:47:08」(JST)

wiki en

[Wiki en表示]

A heteroduplex is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms. One such example is the heteroduplex DNA strand formed in hybridization processes, usually for biochemistry-based phylogenetical analyses. Another example is the heteroduplexes formed when non-natural analogs of nucleic acids are used to bind with nucleic acids; these heteroduplexes result from performing antisense techniques using single-stranded peptide nucleic acid, 2'-O-methyl phosphorothioate or Morpholino oligos to bind with RNA.

In meiosis, the process of crossing-over occurs between non-sister chromatids, which results in new allelic combinations of a population. In crossing-over, a Spo11 enzyme makes staggered nicks in a pair of sister chromatid strands (in a tetrad organization of prophase). Subsequent enzymes trim back the 5' ends of the strand and a protein complex binds to the 3' single-stranded ends. Rad51 protein is recruited and binds in a protein complex to search for a complementary sequence analogous to double-strand-break repair. The filament searches for the homologous chromosome, strand invasion occurs where the new chromosome forms a D-loop over the bottom sister chromatid, then the ends are annealed. This process can yield double Holliday Junctions that when cut in a transversal pattern by endonucleases form 2 heteroduplex strand products.

Heteroduplex DNA is also a source of small RNAs (smRNAs), causing post-transcriptional gene silencing.

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English Journal

• The significance of multiplex PCR/heteroduplex analysis-based TCR-γ gene rearrangement combined with laser-capture microdissection in the diagnosis of early mycosis fungoides.

• Yang H, Xu C, Tang Y, Wan C, Liu W, Wang L.SourceDepartment of Dermatovenerology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China Department of Dermatovenerology, Chongqing the Ninth Hospital, Chongqing, P. R. China Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China Dermatology Hospital of Jiangxi Province, Nanchang, Jiangxi, P. R. China.
• Journal of cutaneous pathology.J Cutan Pathol.2012 Mar;39(3):337-46. doi: 10.1111/j.1600-0560.2011.01842.x.
• Background: The diagnosis of early mycosis fungoides (MF) is a big challenge to dermatologists and dermatopathologists because it lacks specific clinicopathologic features. Methods: Fifty-two paraffin-embedded skin samples from 50 patients, including 31 with suspected MF, 10 with typical MF and 9 wi
• PMID 22335593

• Use of Polymorphisms in the γ-Gliadin Gene of Spelt and Wheat as a Tool for Authenticity Control.

• Mayer F, Haase I, Graubner A, Heising F, Paschke-Kratzin A, Fischer M.SourceHamburg School of Food Science, Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
• Journal of agricultural and food chemistry.J Agric Food Chem.2012 Feb 15;60(6):1350-7. Epub 2012 Feb 7.
• Partial sequencing of the γ-gliadin gene of 62 spelt and 14 soft wheat cultivars was performed. Fifty-six of the 62 spelt cultivars and 13 of the 14 soft wheat cultivars were shown to exhibit the typical spelt or soft wheat γ-gliadin sequence, respectively. Exceptions were ascribed to crossbreedin
• PMID 22264072

Japanese Journal

• Development of a practical NF1 genetic testing method through the pilot analysis of five Japanese families with neurofibromatosis type 1

• Okumura Akiko,Ozaki Mamoru,Niida Yo
• Brain and Development xx(xx), xxxx-xxxx, 2015
• … Methods: Our original method, CEL endonuclease mediated heteroduplex incision with polyacrylamide gel electrophoresis and silver staining (CHIPS) was optimized for NF1 mutation screening, and reverse transcription polymerase chain reaction (RT-PCR) was performed to determine the effect of transcription. … Also, we employed DNA microarray analysis to evaluate the break points of the large deletion. …
• NAID 120005593951

• Screening Methods to Identify TALEN-Mediated Knockout Mice

• Nakagawa Yoshiko,Yamamoto Takashi,Suzuki Ken-Ichi,Araki Kimi,Takeda Naoki,Ohmuraya Masaki,Sakuma Tetsushi
• Experimental Animals 63(1), 79-84, 2014
• … Here, we genotyped TALEN-derived enhanced green fluorescent protein (eGFP) knockout mice using a combination of approaches, including fluorescence observation, heteroduplex mobility assay, restriction fragment length polymorphism analysis and DNA sequencing. …
• NAID 130003391606

• High-resolution melting analysis for a reliable and two-step scanning of mutations in the tyrosine kinase domain of the chimerical bcr-abl gene

• DOI Yuko,SASAKI Daisuke,TERADA Chiharu,MORI Sayaka,TSURUDA Kazuto,MATSUO Emi,MIYAZAKI Yasushi,NAGAI Kazuhiro,HASEGAWA Hiroo,YANAGIHARA Katsunori,YAMADA Yasuaki,KAMIHIRA Shimeru
• International journal of hematology 90(1), 37-43, 2009-07-15
• … Recently, high-resolution melting (HRM) technology was developed, which allows gene scanning using an inexpensive generic heteroduplex-detecting dsDNA-binding dye. … This issue was improved by the addition of exogenous wild-type DNA after PCR. …
• NAID 10025162376

Related Links

• heteroduplex DNA - definition of heteroduplex DNA by The ...

DNA A. adenine T. thymine C. cytosine G. guanine DNA (dē′ĕn-ā′) n. A nucleic acid that carries the genetic information in cells and some viruses, consisting of two long chains of nucleotides twisted into a double helix and joined by ...

• heteroduplex DNA – Dictionary definition of heteroduplex DNA ...

heteroduplex DNA Double-stranded DNA in which the two strands are derived from different DNA molecules. Heteroduplex DNA is formed during genetic recombination (see Holliday intermediate) and can be produced in vitro in DNA ...

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リンク元	「ヘテロ二本鎖DNA」「ヘテロ二重鎖DNA」「nucleic acid heteroduplex」
関連記事	「D」「DNA」「heteroduplex」

「ヘテロ二本鎖DNA」

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英

heteroduplex DNA

関

核酸ヘテロ二重鎖、ヘテロ二重鎖DNA

「ヘテロ二重鎖DNA」

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英

heteroduplex DNA

関

ヘテロ二本鎖DNA

「nucleic acid heteroduplex」

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• 核酸ヘテロ二重鎖

関

heteroduplex DNA

「D」

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• アスパラギン酸
• ジヒドロウリジン
• 遠位の distal,
• うつ病 depression
• 下行結腸 descending colon(大腸内視鏡検査の結果を記載するときに用いる)

　 　

「DNA」

　　[★]

英

deoxyribonucleic acid

同

デオキシリボ核酸

関

リボ核酸 RNA

　

「heteroduplex」

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• ヘテロ二本鎖、ヘテロ二重鎖