heteroduplex

• ヘテロ二本鎖、ヘテロ二重鎖

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/10/19 09:33:19」(JST)

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A heteroduplex is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms.

One such example is the heteroduplex DNA strand formed in hybridization processes, usually for biochemistry-based phylogenetical analyses. Another is the heteroduplexes formed when non-natural analogs of nucleic acids are used to bind with nucleic acids; these heteroduplexes result from performing antisense techniques using single-stranded peptide nucleic acid, 2'-O-methyl phosphorothioate or Morpholino oligos to bind with RNA.

In meiosis, the process of crossing-over occurs between non-sister chromatids, which results in new allelic combinations of a population. In crossing-over, a Spo11 enzyme makes staggered nicks in a pair of sister chromatid strands (in a tetrad organization of prophase). Subsequent enzymes trim back the 5' ends of the strand and a protein complex binds to the 3' single-stranded ends. Rad51 protein is recruited and binds in a protein complex to search for a complementary sequence analogous to double-strand-break repair. The filament searches for the homologous chromosome, strand invasion occurs where the new chromosome forms a D-loop over the bottom sister chromatid, then the ends are annealed. This process can yield double Holliday junctions that when cut in a transversal pattern by endonucleases form 2 heteroduplex strand products.

Heteroduplex DNA is also a source of small RNAs (smRNAs), causing post-transcriptional gene silencing.

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• 1. 遺伝学およびゲノミクスのためのツール：細胞遺伝学および分子遺伝学 tools for genetics and genomics cytogenetics and molecular genetics
• 2. 脳脊髄液リンパ球増加症（HaNDL）を伴う一過性の頭痛および神経障害の症候群 syndrome of transient headache and neurologic deficits with cerebrospinal fluid lymphocytosis handl
• 3. 着床前遺伝子診断 preimplantation genetic diagnosis

English Journal

• Screening Human Genes for Small Alterations Performing an Enzymatic Cleavage Mismatched Analysis (ECMA) Protocol.

• Vogiatzakis N, Kekou K, Sophocleous C, Kitsiou S, Mavrou A, Bakoula C, Kanavakis E.SourceDepartment of Medical Genetics, University of Athens, Athens, Greece.
• Molecular biotechnology.Mol Biotechnol.2013 Sep;55(1):1-9. doi: 10.1007/s12033-007-0062-9. Epub 2007 Aug 14.
• Many human diseases are caused by small alterations in the genes and in the majority of cases sophisticated protocols are required for their detection. In this study we estimated the efficacy of an enzymatic protocol, which using a new mismatch-specific DNA plant endonuclease from celery (CEL family
• PMID 23934597

• Geometric Effect on the Photocrosslinking Reaction between 3-Cyanovinylcarbazole Nucleoside and Pyrimidine Base in DNA/RNA Heteroduplex.

• Fujimoto K, Kishi S, Sakamoto T.SourceSchool of Materials Science, Japan Advanced Institute of Science and Technology, Nomi-shi, Japan; Research Center for Bio-Architecture, Japan Advanced Institute of Science and Technology, Nomi-shi, Japan.
• Photochemistry and photobiology.Photochem Photobiol.2013 Sep;89(5):1095-9. doi: 10.1111/php.12118. Epub 2013 Jul 29.
• To clarify the geometric effect of the ultra-fast photocrosslinking reaction of photoreactive oligodeoxyribonucleotide containing 3-cyanovinylcarbazole nucleoside ((CNV) K) on uridine in complementary RNA strands, pseudouridine (Ψ), which is an isomer of uridine with a C5-C1' glycosidic bond, was i
• PMID 23789821

• Inferring viral population structures using heteroduplex mobility and DNA sequence analyses.

• Shankarappa R, Mullins JI.SourceDepartment of Microbiology, University of Washington School of Medicine, Seattle, WA 98195-8070, United States.
• Journal of virological methods.J Virol Methods.2013 Aug 29. pii: S0166-0934(13)00344-3. doi: 10.1016/j.jviromet.2013.08.012. [Epub ahead of print]
• Heteroduplex mobility (HMA) and tracking assays (HTA) are used to assess genetic relationships between DNA molecules. While distinguishing relationships between clonal or nearly clonal molecules is relatively straightforward, inferring population structures is more complex. To address this issue, HI
• PMID 23994080

Japanese Journal

• High-resolution melting analysis for a reliable and two-step scanning of mutations in the tyrosine kinase domain of the chimerical bcr-abl gene.

• Doi Yuko,Sasaki Daisuke,Terada Chiharu,Mori Sayaka,Tsuruda Kazuto,Matsuo Emi,Miyazaki Yasushi,Nagai Kazuhiro,Hasegawa Hiroo,Yanagihara Katsunori,Yamada Yasuaki,Kamihira Shimeru
• International journal of hematology 90(1), 37-43, 2009-07-15
• … Recently, high-resolution melting (HRM) technology was developed, which allows gene scanning using an inexpensive generic heteroduplex-detecting dsDNA-binding dye. …
• NAID 10025162376

• Efficient Site-selective RNA Activation and Scission Achieved by Geometry Control of Acridine Intercalation in RNA/DNA Heteroduplex

• KUZUYA Akinori,SHI Yun,TANAKA Keita,MACHIDA Kenzo,KOMIYAMA Makoto
• Chemistry letters 38(5), 432-433, 2009-05-05
• NAID 10025121696

• 輸入落花生におけるアフラトキシンBG群汚染とその原因菌類としての Aspergillus section Flavi について

• 岡野 清志,富田 常義,久米田 裕子,松丸 恵子,一戸 正勝
• マイコトキシン 58(2), 107-114, 2008-07-31
• … を超え，AFBG 総量規制では0.4-1.1 ％が規制値を超えていて，ほとんど同様であった．南アフリカ産落花生でも同様で，AFB1 単独規制では0.3-1.0 ％，AFBG 総量規制で0.3-1.2 ％が規制値を超えていた．輸入落花生由来菌につき，AF汚染の原因となるAspergillus section Flavi に所属する菌について形態的，AF およびシクロピアゾン酸の産生性，heteroduplex panel analysis（HPA）による識別を検討したところ，中国産AFBG 検出試料の汚染原因はA. …
• NAID 10021920513

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heteroduplex [-do o ′pleks] Etymology: Gk, heteros + L, duoplicare, to double a DNA molecule in which the two strands are derived from different individuals, with the result that some base pairs may not be complementary. het·er·o·du ...

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ヘテロ二重鎖

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nucleic acid heteroduplex

「nucleic acid heteroduplex」

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