肝実質細胞、肝細胞

関: hepatic cell、hepatocellular、hepatocyte、hepatocytic、liver cell

WordNet

small room in which a monk or nun lives (同)cubicle
a device that delivers an electric current as the result of a chemical reaction (同)electric cell
a room where a prisoner is kept (同)jail cell, prison cell
(biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals
any small compartment; "the cells of a honeycomb"
a small unit serving as part of or as the nucleus of a larger political movement (同)cadre
the primary tissue of higher plants composed of thin-walled cells that remain capable of cell division even when mature; constitutes the greater part of leaves, roots, the pulp of fruits, and the pith of stems
animal tissue that constitutes the essential part of an organ as contrasted with e.g. connective tissue and blood vessels
pertaining to or affecting the liver; "hepatic ducts"; "hepatic cirrhosis"

PrepTutorEJDIC

(刑務所の)『独房』;(修道院の)小さい独居室 / (ミツバチの)みつ房,巣穴 / 小さい部屋 / 『細胞』 / 電池 / 花粉室 / (共産党などの)細胞
肝臓の;肝臓にきく,肝臓のような色の

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2018/06/27 08:42:01」(JST)

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[Wiki en表示]

A hepatocyte is a cell of the main parenchymal tissue of the liver. Hepatocytes make up 70-85% of the liver's mass. These cells are involved in:

Protein synthesis
Protein storage
Transformation of carbohydrates
Synthesis of cholesterol, bile salts and phospholipids
Detoxification, modification, and excretion of exogenous and endogenous substances
Initiation of formation and secretion of bile

Structure

The typical hepatocyte is cubical with sides of 20-30 µm, (in comparison, a human hair has a diameter of 17 to 180 µm).^[1] The typical volume of a hepatocyte is 3.4 x 10⁻⁹ cm³.^[2]

Smooth endoplasmic reticulum is abundant in hepatocytes, whereas most cells in the body have only small amounts.

Microanatomy

Hepatocytes display an eosinophilic cytoplasm, reflecting numerous mitochondria, and basophilic stippling due to large amounts of rough endoplasmic reticulum and free ribosomes. Brown lipofuscin granules are also observed (with increasing age) together with irregular unstained areas of cytoplasm; these correspond to cytoplasmic glycogen and lipid stores removed during histological preparation. The average life span of the hepatocyte is 5 months; they are able to regenerate.

Hepatocyte nuclei are round with dispersed chromatin and prominent nucleoli. Anisokaryosis (or variation in the size of the nuclei) is common and often reflects tetraploidy and other degrees of polyploidy, a normal feature of 30-40% of hepatocytes in the adult human liver.^[3] Binucleate cells are also common.

Hepatocytes are organised into plates separated by vascular channels (sinusoids), an arrangement supported by a reticulin (collagen type III) network. The hepatocyte plates are one cell thick in mammals and two cells thick in the chicken. Sinusoids display a discontinuous, fenestrated endothelial cell lining. The endothelial cells have no basement membrane and are separated from the hepatocytes by the space of Disse, which drains lymph into the portal tract lymphatics.

Kupffer cells are scattered between endothelial cells; they are part of the reticuloendothelial system and phagocytose spent erythrocytes. Stellate (Ito) cells store vitamin A and produce extracellular matrix and collagen; they are also distributed amongst endothelial cells but are difficult to visualise by light microscopy.

Function

Protein synthesis

The hepatocyte is a cell in the body that manufactures serum albumin, fibrinogen, and the prothrombin group of clotting factors (except for Factors 3 and 4).

It is the main site for the synthesis of lipoproteins, ceruloplasmin, transferrin, complement, and glycoproteins. Hepatocytes manufacture their own structural proteins and intracellular enzymes.

Synthesis of proteins is by the rough endoplasmic reticulum (RER), and both the rough and smooth endoplasmic reticulum (SER) are involved in secretion of the proteins formed.

The endoplasmic reticulum (ER) is involved in conjugation of proteins to lipid and carbohydrate moieties synthesized by, or modified within, the hepatocytes.

Carbohydrate metabolism

The liver forms fatty acids from carbohydrates and synthesizes triglycerides from fatty acids and glycerol.^[4] Hepatocytes also synthesize apoproteins with which they then assemble and export lipoproteins (VLDL, HDL).

The liver is also the main site in the body for gluconeogenesis, the formation of carbohydrates from precursors such as alanine, glycerol, and oxaloacetate.

Lipid metabolism

The liver receives many lipids from the systemic circulation and metabolizes chylomicron remnants. It also synthesizes cholesterol from acetate and further synthesizes bile salts. The liver is the sole site of bile salts formation.

Detoxification

Hepatocytes have the ability to metabolize, detoxify, and inactivate exogenous compounds such as drugs, (drug metabolism), and insecticides, and endogenous compounds such as steroids.

The drainage of the intestinal venous blood into the liver requires efficient detoxification of miscellaneous absorbed substances to maintain homeostasis and protect the body against ingested toxins.

One of the detoxifying functions of hepatocytes is to modify ammonia into urea for excretion.

The most abundant organelle in liver cell is the smooth endoplasmic reticulum.

Society and culture

Use in research

Primary hepatocytes are commonly used in cell biological and biopharmaceutical research. In vitro model systems based on hepatocytes have been of great help to better understand the role of hepatocytes in (patho)physiological processes of the liver. In addition, pharmaceutical industry has heavily relied on the use of hepatocytes in suspension or culture to explore mechanisms of drug metabolism and even predict in vivo drug metabolism. For these purposes, hepatocytes are usually isolated from animal or human^[5] whole liver or liver tissue by collagenase digestion, which is a two-step process. In the first step, the liver is placed in an isotonic solution, in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent. Next, a solution containing collagenase is added to separate the hepatocytes from the liver stroma. This process creates a suspension of hepatocytes, which can be seeded in multi-well plates and cultured for many days or even weeks. For optimal results, culture plates should first be coated with an extracellular matrix (e.g. collagen, Matrigel) to promote hepatocyte attachment (typically within 1-3 hr after seeding) and maintenance of the hepatic phenotype. In addition, and overlay with an additional layer of extracellular matrix is often performed to establish a sandwich culture of hepatocytes. The application of a sandwich configuration supports prolonged maintenance of hepatocytes in culture.^[6]^[7] Freshly-isolated hepatocytes that are not used immediately can be cryopreserved and stored.^[8] They do not proliferate in culture. Hepatocytes are intensely sensitive to damage during the cycles of cryopreservation including freezing and thawing. Even after the addition of classical cryoprotectants there is still damage done while being cryopreserved.^[9] Nevertheless, recent cryopreservation and resuscitation protocols support application of cryopreserved hepatocytes for most biopharmaceutical applications.^[10]

Additional images

Schemic diagram of Biliary system

References

^ Ley, Brian (1999). "Diameter of a Human Hair". Archived from the original on 2010-06-28. Retrieved 2010-06-28.
^ Lodish, H., Berk, A., Zipursky, S. L., Matsudaira, P., Baltimore, D., Darnell, J. E. Molecular Cell Biology (Fifth Edition). W. H. Freeman and Company. New York, 2000, pp 10.
^ Celton-Morizur, S; Merlen, G; Couton, D; Desdouets, C (1 February 2010). "Polyploidy and liver proliferation: central role of insulin signaling" (PDF). Cell cycle (Georgetown, Tex.). 9 (3): 460–6. doi:10.4161/cc.9.3.10542. PMID 20090410. Archived from the original (PDF) on 2012-04-25.
^ Ali ES, Hua J, Wilson CH, Tallis GA, Zhou FH, Rychkov GY, Barritt GJ (2016). "The glucagon-like peptide-1 analogue exendin-4 reverses impaired intracellular Ca2+ signalling in steatotic hepatocytes". BBA − Molecular Cell Research. 1863: 2135–46. doi:10.1016/j.bbamcr.2016.05.006. PMID 27178543.
^ Lecluyse EL, Alexandre E (2010). "Isolation and culture of primary hepatocytes from resected human liver tissue". Methods Mol Biol. 640: 57–82. doi:10.1007/978-1-60761-688-7_3. PMID 20645046.
^ Dunn JC, Yarmush ML, Koebe HG, Tompkins RG (Feb 1989). "Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration". FASEB J. 3 (2): 174–7. Erratum in: FASEB J 1989 May;3(7):1873. PMID 2914628.
^ De Bruyn T, Chatterjee S, Fattah S, Keemink J, Nicolaï J, Augustijns P, Annaert P (May 2013). "Sandwich-cultured hepatocytes: utility for in vitro exploration of hepatobiliary drug disposition and drug-induced hepatotoxicity". Expert Opin Drug Metab Toxicol. 9 (5): 589–616. doi:10.1517/17425255.2013.773973. PMID 23452081.
^ Li Albert P (2001). "Screening for human ADME/Tox drug properties in Drug Discovery". Drug Discovery Today. 6 (7): 357–366. doi:10.1016/s1359-6446(01)01712-3.
^ Hamel, F.; Grondin, M. L.; Denizeau, F.; Averill-Bates, D. A.; Sarhan, F. (2006). "Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes". Biotechnology and Bioengineering. 95 (4): 661–670. doi:10.1002/bit.20953. PMID 16927246.
^ De Bruyn T, Ye ZW, Peeters A, Sahi J, Baes M, Augustijns PF, Annaert PP (Jul 2011). "Determination of OATP-, NTCP- and OCT-mediated substrate uptake activities in individual and pooled batches of cryopreserved human hepatocytes". Eur J Pharm Sci. 43 (4): 297–307. doi:10.1016/j.ejps.2011.05.002. PMID 21605667.

External links

Histology image: 22101ooa – Histology Learning System at Boston University - "Ultrastructure of the Cell: hepatocytes and sinusoids"
Hepatic Histology: Hepatocytes (Colorado State University

UpToDate Contents

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English Journal

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We have prepared a novel nanobiotherapeutic, Poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase], which not only transports both oxygen and carbon dioxide but also a therapeutic antioxidant. Our previous study in a severe sustained 90 min hemorrhagic shock rat model shows that it ha
PMID 27263665

Japanese Journal

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NAID 120006373804

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NAID 130006827256

Related Pictures

★リンクテーブル★

リンク元	「肝実質細胞」「hepatocellular」「liver cell」「hepatocytic」「hepatic cell」
関連記事	「hepatic」「parenchymal」「parenchyma」「cell」「parenchymal cell」

「肝実質細胞」

Hepatocyte
	Hepatocyte and sinusoid (venule) in a section of rat liver
Details
Location	Liver
Identifiers
TH	H3.04.05.0.00006
	Anatomical terms of microanatomy; [edit on Wikidata]