"COX" redirects here. For other uses, see Cox (disambiguation).
| prostaglandin-endoperoxide synthase |
| Identifiers |
| EC number |
1.14.99.1 |
| CAS number |
9055-65-6 |
| Databases |
| IntEnz |
IntEnz view |
| BRENDA |
BRENDA entry |
| ExPASy |
NiceZyme view |
| KEGG |
KEGG entry |
| MetaCyc |
metabolic pathway |
| PRIAM |
profile |
| PDB structures |
RCSB PDB PDBe PDBsum |
| Gene Ontology |
AmiGO / QuickGO |
| Search |
| PMC |
articles |
| PubMed |
articles |
| NCBI |
proteins |
|
| cyclooxygenase 1 |
|
Crystallographic structure of prostaglandin H2 synthase-1 complex with flurbiprofen.[1]
|
| Identifiers |
| Symbol |
PTGS1 |
| Alt. symbols |
COX-1 |
| Entrez |
5742 |
| HUGO |
9604 |
| OMIM |
176805 |
| PDB |
1CQE |
| RefSeq |
NM_080591 |
| UniProt |
P23219 |
| Other data |
| EC number |
1.14.99.1 |
| Locus |
Chr. 9 q32-q33.3 |
| cyclooxygenase 2 |
|
Cyclooxygenase-2 (Prostaglandin Synthase-2) in complex with a COX-2 selective inhibitor.[2]
|
| Identifiers |
| Symbol |
PTGS2 |
| Alt. symbols |
COX-2 |
| Entrez |
5743 |
| HUGO |
9605 |
| OMIM |
600262 |
| PDB |
6COX |
| RefSeq |
NM_000963 |
| UniProt |
P35354 |
| Other data |
| EC number |
1.14.99.1 |
| Locus |
Chr. 1 q25.2-25.3 |
Cyclooxygenase (COX), officially known as prostaglandin-endoperoxide synthase (PTGS), is an enzyme (specifically, a family of isozymes, EC 1.14.99.1) that is responsible for formation of prostanoids, including thromboxane and prostaglandins such as prostacyclin, from arachidonic acid.[3]
Pharmaceutical inhibition of COX can provide relief from the symptoms of inflammation and pain.[3] Nonsteroidal anti-inflammatory drugs (NSAIDs), such as aspirin and ibuprofen, exert their effects through inhibition of COX. Those that are specific to the COX-2 isozyme are called COX-2 inhibitors. The active metabolite (AM404) of paracetamol believed to provide most or all of its analgesic effects is a COX inhibitor and this is believed to provide part of its effect.[4]
In medicine, the root symbol "COX" is encountered more often than "PTGS". In genetics, "PTGS" is officially used for this family of genes and proteins, because the root symbol "COX" was already used for the cytochrome c oxidase family. Thus the two isozymes found in humans, PTGS1 and PTGS2, are frequently called COX-1 and COX-2 in the medical literature. The names "prostaglandin synthase (PHS)", "prostaglandin synthetase (PHS)", and "prostaglandin-endoperoxide synthetase (PES)" are older terms still sometimes used to refer to COX.
Contents
- 1 Pharmacology
- 1.1 Classical NSAIDs
- 1.2 Newer NSAIDs
- 1.3 Natural COX inhibition
- 1.4 Cardiovascular side-effects of COX inhibitors
- 2 See also
- 3 References
- 4 External links
Pharmacology
In terms of their molecular biology, COX-1 and COX-2 are of similar molecular weight, approximately 70 and 72 kDa, respectively, and having 65% amino acid sequence homology and near-identical catalytic sites. The most significant difference between the isoenzymes, which allows for selective inhibition, is the substitution of isoleucine at position 523 in COX-1 with valine in COX-2. The smaller Val523 residue in COX-2 allows access to a hydrophobic side-pocket in the enzyme (which Ile523 sterically hinders). Drug molecules, such as DuP-697 and the coxibs derived from it, bind to this alternative site and are considered to be selective inhibitors of COX-2.
Classical NSAIDs
See also: Mechanism of action of aspirin
The main COX inhibitors are the non-steroidal anti-inflammatory drugs (NSAIDs).
The classical COX inhibitors are not selective and inhibit all types of COX. The resulting inhibition of prostaglandin and thromboxane synthesis has the effect of reduced inflammation, as well as antipyretic, antithrombotic and analgesic effects. The most frequent adverse effect of NSAIDs is irritation of the gastric mucosa as prostaglandins normally have a protective role in the gastrointestinal tract. Some NSAIDs are also acidic which may cause additional damage to the gastrointestinal tract.
Newer NSAIDs
Selectivity for COX-2 is the main feature of celecoxib, etoricoxib, and other members of this drug class. Because COX-2 is usually specific to inflamed tissue, there is much less gastric irritation associated with COX-2 inhibitors, with a decreased risk of peptic ulceration. The selectivity of COX-2 does not seem to negate other side-effects of NSAIDs, most notably an increased risk of renal failure, and there is evidence that indicates an increase in the risk of heart attack, thrombosis, and stroke through an increase of thromboxane unbalanced by prostacyclin (which is reduced by COX-2 inhibition).[citation needed] Rofecoxib (brand name Vioxx) was withdrawn in 2004 because of such concerns. Some other COX-2 selective NSAIDs, such as celecoxib, and etoricoxib, are still on the market.
Natural COX inhibition
Culinary mushrooms, like maitake, may be able to partially inhibit COX-1 and COX-2.[5][6]
A variety of flavonoids have been found to inhibit COX-2.[7]
Fish oils provide alternative fatty acids to arachidonic acid. These acids can be turned into some anti-inflammatory prostacyclins by COX instead of pro-inflammatory prostaglandins.[8]
Hyperforin has been shown to inhibit COX-1 around 3-18 times as much as aspirin.[9]
Calcitriol (vitamin D) significantly inhibits the expression of the COX-2 gene.[10]
Caution should be exercised in combining low dose aspirin with COX-2 inhibitors due to potential increased damage to the gastric mucosa. COX-2 is upregulated when COX-1 is suppressed with aspirin, which is thought to be important in enhancing mucosal defense mechanisms and lessening the erosion by aspirin.[11]
Cardiovascular side-effects of COX inhibitors
COX-2 inhibitors have been found to increase the risk of atherothrombosis even with short-term use. A 2006 analysis of 138 randomised trials and almost 150 000 participants[12] showed that selective COX-2 inhibitors are associated with a moderately increased risk of vascular events, mainly due to a twofold increased risk of myocardial infarction, and also that high-dose regimens of some traditional NSAIDs (such as diclofenac and ibuprofen, but not naproxen) are associated with a similar increase in risk of vascular events.
Fish oils (e.g., cod liver oil) have been proposed as a reasonable alternative for the treatment of rheumatoid arthritis and other conditions as a consequence of the fact that they provide less cardiovascular risk than other treatments including NSAIDs.[8]
See also
- Arachidonic acid
- Cyclooxygenase 1
- Cyclooxygenase 2
- NSAID
- Discovery and development of COX-2 selective inhibitors
- COX-2 selective inhibitor
- COX-3 (not functional in humans)
References
- ^ PDB: 1CQE; Picot D, Loll PJ, Garavito RM (January 1994). "The X-ray crystal structure of the membrane protein prostaglandin H2 synthase-1". Nature. 367 (6460): 243–9. doi:10.1038/367243a0. PMID 8121489.
- ^ PDB: 6COX; Kurumbail RG, Stevens AM, Gierse JK, McDonald JJ, Stegeman RA, Pak JY, Gildehaus D, Miyashiro JM, Penning TD, Seibert K, Isakson PC, Stallings WC (1996). "Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agents". Nature. 384 (6610): 644–8. doi:10.1038/384644a0. PMID 8967954.
- ^ a b Litalien C, Beaulieu P (2011). "Chapter 117 – Molecular Mechanisms of Drug Actions: From Receptors to Effectors". In Fuhrman BP, Zimmerman JJ. Pediatric Critical Care (4th ed.). Philadelphia, PA: Elsevier Saunders. pp. 1553–1568. doi:10.1016/B978-0-323-07307-3.10117-X. ISBN 978-0-323-07307-3.
Arachidonic acid is a component of membrane phospholipids released either in a one-step process, after phospholipase A2 (PLA2) action, or a two-step process, after phospholipase C and DAG lipase actions. Arachidonic acid is then metabolized by cyclooxygenase (COX) and 5-lipoxygenase, resulting in the synthesis of prostaglandins and leukotrienes, respectively. These intracellular messengers play an important role in the regulation of signal transduction implicated in pain and inflammatory responses.
- ^ Högestätt ED, Jönsson BA, Ermund A, Andersson DA, Björk H, Alexander JP, Cravatt BF, Basbaum AI, Zygmunt PM (September 2005). "Conversion of acetaminophen to the bioactive N-acylphenolamine AM404 via fatty acid amide hydrolase-dependent arachidonic acid conjugation in the nervous system" (pdf). The Journal of Biological Chemistry. 280 (36): 31405–12. doi:10.1074/jbc.M501489200. PMID 15987694.
- ^ Zhang Y, Mills GL, Nair MG (December 2002). "Cyclooxygenase inhibitory and antioxidant compounds from the mycelia of the edible mushroom Grifola frondosa". Journal of Agricultural and Food Chemistry. 50 (26): 7581–5. doi:10.1021/jf0257648. PMID 12475274.
- ^ Zhang Y, Mills GL, Nair MG (2003). "Cyclooxygenase inhibitory and antioxidant compounds from the fruiting body of an edible mushroom, Agrocybe aegerita". Phytomedicine. 10 (5): 386–90. doi:10.1078/0944-7113-00272. PMID 12834003.
- ^ O'Leary KA, de Pascual-Teresa S, de Pascual-Tereasa S, Needs PW, Bao YP, O'Brien NM, Williamson G (July 2004). "Effect of flavonoids and vitamin E on cyclooxygenase-2 (COX-2) transcription". Mutation Research. 551 (1-2): 245–54. doi:10.1016/j.mrfmmm.2004.01.015. PMID 15225597.
- ^ a b Cleland LG, James MJ, Proudman SM (2006). "Fish oil: what the prescriber needs to know". Arthritis Research & Therapy. 8 (1): 202. doi:10.1186/ar1876. PMC 1526555 . PMID 16542466.
- ^ Albert D, Zündorf I, Dingermann T, Müller WE, Steinhilber D, Werz O (December 2002). "Hyperforin is a dual inhibitor of cyclooxygenase-1 and 5-lipoxygenase". Biochemical Pharmacology. 64 (12): 1767–75. doi:10.1016/s0006-2952(02)01387-4. PMID 12445866.
- ^ Moreno J, Krishnan AV, Peehl DM, Feldman D (July–August 2006). "Mechanisms of vitamin D-mediated growth inhibition in prostate cancer cells: inhibition of the prostaglandin pathway". Anticancer Research. 26 (4A): 2525–30. PMID 16886660.
- ^ Wallace JL (October 2008). "Prostaglandins, NSAIDs, and gastric mucosal protection: why doesn't the stomach digest itself?". Physiological Reviews. 88 (4): 1547–65. doi:10.1152/physrev.00004.2008. PMID 18923189.
- ^ Kearney PM, Baigent C, Godwin J, Halls H, Emberson JR, Patrono C (June 2006). "Do selective cyclo-oxygenase-2 inhibitors and traditional non-steroidal anti-inflammatory drugs increase the risk of atherothrombosis? Meta-analysis of randomised trials". BMJ. 332 (7553): 1302–8. doi:10.1136/bmj.332.7553.1302. PMC 1473048 . PMID 16740558.
External links
- The Cyclooxygenase Protein
- Cyclooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)
- GONUTS Page: Cyclooxygenase
- Cyclooxygenase: Proteopedia, life in 3D
- A discussion of the enzymatic mechanism, including interactive 3D models[permanent dead link]
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Metabolism: lipid metabolism - eicosanoid metabolism enzymes
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| Precursor |
- Phospholipase C
- Diacylglycerol lipase
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| Prostanoids |
- PGE synthase
- Prostaglandin-E2 9-reductase
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| Leukotrienes |
- 5-Lipoxygenase activating protein/Arachidonate 5-lipoxygenase
- LTA4 hydrolase (B4 synthesis)
- LTC4 synthase
- Gamma-glutamyl transpeptidase
- LTD4 hydrolase
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| Ungrouped |
|
|
Prostanoid signaling modulators
|
Receptor
(ligands) |
|
Enzyme
(inhibitors) |
COX
(PTGS) |
|
| PGD2S |
- Retinoids
- Selenium (selenium tetrachloride, sodium selenite, selenium disulfide)
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| PGES |
HQL-79
|
| PGFS |
Bimatoprost
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| PGI2S |
Tranylcypromine
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| TXAS |
- Camonagrel
- Dazmegrel
- Dazoxiben
- Furegrelate
- Isbogrel
- Midazogrel
- Nafagrel
- Nicogrelate
- Ozagrel
- Picotamide
- Pirmagrel
- Ridogrel
- Rolafagrel
- Samixogrel
- Terbogrel
- U63557A
|
|
| Others |
- Precursors: Linoleic acid
- γ-Linolenic acid (gamolenic acid)
- Dihomo-γ-linolenic acid
- Diacylglycerol
- Arachidonic acid
- Prostaglandin G2
- Prostaglandin H2
|
- See also
- Receptor/signaling modulators
- Leukotriene signaling modulators
- Nuclear receptor modulators
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|
Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14)
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| 1.14.11: 2-oxoglutarate |
- Prolyl hydroxylase
- Lysyl hydroxylase
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| 1.14.13: NADH or NADPH |
- Flavin-containing monooxygenase
- Nitric oxide synthase
- Cholesterol 7 alpha-hydroxylase
- Methane monooxygenase
- 3A4
- Lanosterol 14 alpha-demethylase
- 24-hydroxycholesterol 7α-hydroxylase
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| 1.14.14: reduced flavin or flavoprotein |
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| 1.14.15: reduced iron-sulfur protein |
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| 1.14.16: reduced pteridine (BH4 dependent) |
- Phenylalanine hydroxylase
- Tyrosine hydroxylase
- Tryptophan hydroxylase
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| 1.14.17: reduced ascorbate |
- Dopamine beta-hydroxylase
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| 1.14.18-19: other |
- Tyrosinase
- Stearoyl-CoA desaturase-1
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| 1.14.99 - miscellaneous |
- Cyclooxygenase
- Heme oxygenase (HMOX1)
- Squalene monooxygenase
- 17A1
- 21A2
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|
Enzymes
|
| Activity |
- Active site
- Binding site
- Catalytic triad
- Oxyanion hole
- Enzyme promiscuity
- Catalytically perfect enzyme
- Coenzyme
- Cofactor
- Enzyme catalysis
|
| Regulation |
- Allosteric regulation
- Cooperativity
- Enzyme inhibitor
- Enzyme activator
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| Classification |
- EC number
- Enzyme superfamily
- Enzyme family
- List of enzymes
|
| Kinetics |
- Enzyme kinetics
- Eadie–Hofstee diagram
- Hanes–Woolf plot
- Lineweaver–Burk plot
- Michaelis–Menten kinetics
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| Types |
- EC1 Oxidoreductases (list)
- EC2 Transferases (list)
- EC3 Hydrolases (list)
- EC4 Lyases (list)
- EC5 Isomerases (list)
- EC6 Ligases (list)
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