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pH-dependent effect of pectinase secretion in Penicillium griseoroseum recombinant strains.

Teixeira JA, Corrêa TL, de Queiroz MV, de Araújo EF.SourceDepartment of Microbiology/BIOAGRO, Federal University of Viçosa (Universidade Federal de Viçosa), Viçosa-MG, Brazil.
Journal of basic microbiology.J Basic Microbiol.2013 Apr 2. doi: 10.1002/jobm.201200325. [Epub ahead of print]
A number of parameters, including culture medium pH, affect growth and enzyme production by microorganisms. In the present study, the production and secretion of pectin lyase (PL) and polygalacturonase (PG) by recombinant strains of Penicillium griseoroseum cultured in mineral-buffered media (MBM; i
PMID 23553535

Structural and catalytic insights into the algal prostaglandin H synthase reveal atypical features of the first non-animal cyclooxygenase.

Varvas K, Kasvandik S, Hansen K, Järving I, Morell I, Samel N.SourceDepartment of Chemistry, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
Biochimica et biophysica acta.Biochim Biophys Acta.2013 Apr;1831(4):863-71. doi: 10.1016/j.bbalip.2012.11.010. Epub 2012 Dec 5.
Prostaglandin H synthases (PGHSs) have been identified in the majority of vertebrate and invertebrate animals, and most recently in the red alga Gracilaria vermiculophylla. Here we report on the cloning, expression and characterization of the algal PGHS, which shares only about 20% of the amino acid
PMID 23220097

Overproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene.

Teixeira JA, Ribeiro JB, Gonçalves DB, de Queiroz MV, de Araújo EF.SourceDepartamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Applied biochemistry and biotechnology.Appl Biochem Biotechnol.2013 Mar;169(6):1965-77. doi: 10.1007/s12010-013-0121-6. Epub 2013 Jan 26.
Inactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase
PMID 23354503