antigen-binding fragment

• 抗原結合性フラグメント

関

Fab、Fab fragment

WordNet

1. executed with proper legal authority; "a binding contract"
2. the protective covering on the front, back, and spine of a book; "the book had a leather binding" (同)book binding, cover, back
3. strip sewn over or along an edge for reinforcement or decoration
4. the capacity to attract and hold something
5. a piece broken off or cut off of something else; "a fragment of rock"
6. an incomplete piece; "fragments of a play"
7. any substance (as a toxin or enzyme) that stimulates an immune response in the body (especially the production of antibodies)

PrepTutorEJDIC

1. 義務的な,拘束力ある / 〈U〉しばること;〈C〉しばる物 / 〈C〉製本,装丁 / 〈U〉縁(‘ふち')取り材料
2. (…の)『破片』,断片《+『of』+『名』》 / (特に詩文などの)未完の部分,断章《+『of』+『名』》 / ばらばらになる,砕ける / …‘を'ばらばらに壊す
3. 抗原(生物の体内にはいって免疫体を作る物質)

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2017/03/01 15:30:03」(JST)

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[Wiki en表示]

An antibody digested by papain yields three fragments: two Fab fragments and one Fc fragment

An antibody digested by pepsin yields two fragments: a F(ab')₂ fragment and a pFc' fragment

The fragment antigen-binding (Fab) fragment is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. The variable domain contains the paratope (the antigen-binding site), comprising a set of complementarity determining regions, at the amino terminal end of the monomer. Each arm of the Y thus binds an epitope on the antigen.

In an experimental setting, Fc and Fab fragments can be generated in the laboratory. The enzyme papain can be used to cleave an immunoglobulin monomer into two Fab fragments and an Fc fragment. The enzyme pepsin cleaves below the hinge region, so a F(ab')₂ fragment and a pFc' fragment is formed. Recently another enzyme for generation of F(ab')₂ has been commercially available. The enzyme IdeS (Immunoglobulin degrading enzyme from Streptococcus pyogenes, trade name FabRICATOR) cleaves IgG in a sequence specific manner at neutral pH. The F(ab')₂ fragment can be split into two Fab' fragments by mild reduction.

The variable regions of the heavy and light chains can be fused together to form a single-chain variable fragment (scFv), which is only half the size of the Fab fragment, yet retains the original specificity of the parent immunoglobulin.

Heavy and light chains, variable and constant regions of an antibody.

See also

• Fc region

References

UpToDate Contents

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• 1. 免疫グロブリンの機能および臨床応用 function and clinical applications of immunoglobulins
• 2. 免疫グロブリンの構造 structure of immunoglobulins
• 3. 体液性免疫応答 the humoral immune response
• 4. Antigen-presenting cells
• 5. 補体経路 complement pathways

English Journal

• Photoimmunotherapy targeting prostate-specific membrane antigen: are antibody fragments as effective as antibodies?

• Watanabe R1, Hanaoka H1, Sato K1, Nagaya T1, Harada T1, Mitsunaga M1, Kim I2, Paik CH3, Wu AM4, Choyke PL1, Kobayashi H5.
• Journal of nuclear medicine : official publication, Society of Nuclear Medicine.J Nucl Med.2015 Jan;56(1):140-4. doi: 10.2967/jnumed.114.149526. Epub 2014 Dec 11.
• Photoimmunotherapy is a highly cell-selective cancer therapy based on an armed antibody conjugate with a phthalocyanine-based photosensitizer, IR700. Photoimmunotherapy induces rapid and highly specific necrosis in targeted cancer cells after exposure to near-infrared (NIR) light. Cells not expressi
• PMID 25500827

• Effects of Fab' fragments of specific egg yolk antibody (IgY-Fab') against Shewanella putrefaciens on the preservation of refrigerated turbot.

• Zhang Q1, Lin H, Sui J, Wang J, Cao L.
• Journal of the science of food and agriculture.J Sci Food Agric.2015 Jan;95(1):136-40. doi: 10.1002/jsfa.6693. Epub 2014 May 21.
• BACKGROUND: In our previous studies the specific egg yolk antibody (IgY) against Shewanella putrefaciens (one of the specific spoilage organisms for marine products during aerobic chilling storage) demonstrated significant activity to prolong the shelf life of refrigerated fish. The exploitation of
• PMID 24737722

• Enhanced production of functional extracellular single chain variable fragment against HIV-1 matrix protein from Escherichia coli by sequential simplex optimization.

• Intachai K1, Singboottra P, Leksawasdi N, Kasinrerk W, Tayapiwatana C, Butr-Indr B.
• Preparative biochemistry & biotechnology.Prep Biochem Biotechnol.2015;45(1):56-68. doi: 10.1080/10826068.2014.887580.
• The optimal culture condition for extracellular recombinant single chain variable fragment anti HIV-1 p17 protein (scFv anti-p17) production in Escherichia coli HB2151 was investigated by the sequential simplex optimization (SS) method. Five variable parameters were submitted in the fermentation pro
• PMID 24884357

Japanese Journal

• Double-Fluorescent-Labeled Single-Chain Antibodies Showing Antigen-Dependent Fluorescence Ratio Change

• Bulletin of the Chemical Society of Japan 89(5), 573-580, 2016
• NAID 130005150877

• Double-Fluorescent-Labeled Single-Chain Antibodies Showing Antigen-Dependent Fluorescence Ratio Change

• Bulletin of the Chemical Society of Japan advpub(0), 2016
• NAID 130005124580

• 1S-Ep02 抗原結合により光る抗体Q-bodyの開発とそのバイオ計測への応用(抗体工学の新潮流:シーズからニーズへ,シンポジウム)

• 日本生物工学会大会講演要旨集 67, 168, 2015-09-25
• NAID 110010009080

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• Antigen binding fragment - ウィキまとめ

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「抗原結合性フラグメント」

　　[★]

英

antigen-binding fragment、Fab、Fab fragment

関

Fabフラグメント、免疫グロブリンFabフラグメント

「binding」

　　[★]

• n.

• 結合、バインディング

• adj.

• 結合性の

関

affinity、associate、bind、bond、bonding、combine、conjoin、conjugate、conjugation、conjunction、connect、connection、connective、connectivity、couple、dock、engage、engagement、join、ligate、linkage、symphysial、symphysic、union

　 　 　 　 　

「fragment」

　　[★]

• n.

• 断片、破片、フラグメント

• v.

• 断片化する、細分化する

関

fragmentation、piece、shred、sliver、splinter、subdivision