DNAポリメラーゼα
- 関
- DNAポリメラーゼI、DNA polymerase I、Klenow fragment、Pol I
WordNet
- first in order of importance; "the alpha male in the group of chimpanzees"; "the alpha star in a constellation is the brightest or main star"
- the 1st letter of the Greek alphabet
- the beginning of a series or sequence; "the Alpha and Omega, the first and the last, the beginning and the end"--Revelations
- early testing stage of a software or hardware product; "alpha version"
- the 4th letter of the Roman alphabet (同)d
- an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA
- any high mountain
PrepTutorEJDIC
- アルファ(ギリシア語アルファベットの第1字A,α;英語のA,aに相当) / アルファ星(星座の主星)
- deuteriumの化学記号
- deoxyribonucleic acidディオキシリボ核酸
- 高山,高峰
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/11/21 21:35:18」(JST)
[Wiki en表示]
| DNA-directed DNA polymerase |
| Identifiers |
| EC number |
2.7.7.7 |
| CAS number |
9012-90-2 |
| Databases |
| IntEnz |
IntEnz view |
| BRENDA |
BRENDA entry |
| ExPASy |
NiceZyme view |
| KEGG |
KEGG entry |
| MetaCyc |
metabolic pathway |
| PRIAM |
profile |
| PDB structures |
RCSB PDB PDBe PDBsum |
| Search |
| PMC |
articles |
| PubMed |
articles |
| NCBI |
proteins |
|
DNA polymerase alpha also known as Pol α is an enzyme complex found in eukaryotes that is involved in initiation of DNA replication. The DNA polymerase alpha complex consists of 4 subunits: POLA1, POLA2, PRIM1, and PRIM2.[1]
Pol α has limited processivity and lacks 3′ exonuclease activity for proofreading errors. Thus it is not well suited to efficiently and accurately copy long templates (unlike Pol Delta and Epsilon). Instead it plays a more limited role in replication. Pol α is responsible for the initiation of DNA replication at origins of replication (on both the leading and lagging strands) and during synthesis of Okazaki fragments on the lagging strand. The Pol α complex (pol α-DNA primase complex) consists of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2 respectively. Once primase has created the RNA primer, Pol α starts replication elongating the primer with ~20 nucleotides.[1]
References
- ^ a b Lehman IR, Kaguni LS (March 1989). "DNA polymerase alpha" (PDF). J. Biol. Chem. 264 (8): 4265–8. PMID 2647732.
External links
- DNA polymerase alpha at the US National Library of Medicine Medical Subject Headings (MeSH)
|
DNA replication (comparing Prokaryotic to Eukaryotic)
|
|
| Initiation |
Prokaryotic
(initiation) |
|
|
Eukaryotic
(preparation in
G1 phase) |
- Origin recognition complex
- ORC1
- ORC2
- ORC3
- ORC4
- ORC5
- ORC6
- Minichromosome maintenance
- MCM2
- MCM3
- MCM4
- MCM5
- MCM6
- MCM7
- Autonomously replicating sequence
- Single-strand binding protein
|
|
| Both |
- Origin of replication/Ori/Replicon
- Replication fork
- Lagging and leading strands
- Okazaki fragments
- Primer
|
|
|
| Replication |
Prokaryotic
(elongation) |
- DNA polymerase III holoenzyme
- dnaC
- dnaE
- dnaH
- dnaN
- dnaQ
- dnaT
- dnaX
- holA
- holB
- holC
- holD
- holE
- Replisome
- DNA ligase
- DNA clamp
- Topoisomerase
- Prokaryotic DNA polymerase: DNA polymerase I
|
|
Eukaryotic
(synthesis in
S phase) |
- Replication factor C
- Flap endonuclease
- Topoisomerase
- Replication protein A
- Eukaryotic DNA polymerase:
- alpha
- delta
- epsilon
- Control of chromosome duplication
|
|
| Both |
- Movement: Processivity
- DNA ligase
|
|
|
| Termination |
|
|
|
Index of genetics
|
|
| Description |
- Gene expression
- DNA
- replication
- cycle
- recombination
- repair
- binding proteins
- Transcription
- factors
- regulators
- nucleic acids
- RNA
- RNA binding proteins
- ribonucleoproteins
- repeated sequence
- modification
- Translation
- ribosome
- modification
- nexins
- Proteins
- domains
- Structure
- primary
- secondary
- tertiary
- quaternary
|
|
| Disease |
- Replication and repair
- Transcription factor
- Transcription
- Translation
|
|
|
This article incorporates text from the United States National Library of Medicine, which is in the public domain.
UpToDate Contents
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English Journal
- Tracking Mitochondrial DNA In Situ.
- Ligasová A1, Koberna K2.
- Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2016;1351:81-92. doi: 10.1007/978-1-4939-3040-1_7.
- The methods of the detection of (1) non-labeled and (2) BrdU-labeled mitochondrial DNA (mtDNA) are described. They are based on the production of singlet oxygen by monovalent copper ions and the subsequent induction of DNA gaps. The ends of interrupted DNA serve as origins for the labeling of mtDNA
- PMID 26530676
- Molecular mechanism of anticancer effect of Sclerotium rolfsii lectin in HT29 cells involves differential expression of genes associated with multiple signaling pathways: A microarray analysis.
- Barkeer S1, Guha N2, Hothpet V1, Saligrama Adavigowda D2, Hegde P1, Padmanaban A2, Yu LG3, Swamy BM4, Inamdar SR5.
- Glycobiology.Glycobiology.2015 Dec;25(12):1375-91. doi: 10.1093/glycob/cwv067. Epub 2015 Sep 7.
- Sclerotium rolfsii lectin (SRL) is a lectin isolated from fungus S. rolfsii and has high binding specificity toward the oncofetal Thomsen-Friedenreich carbohydrate antigen (Galβ1-3GalNAc-α-O-Ser/Thr, T or TF), which is expressed in more than 90% of human cancers. Our previous studies have shown th
- PMID 26347523
- TG1050, an immunotherapeutic to treat chronic hepatitis B, induces robust T cells and exerts an antiviral effect in HBV-persistent mice.
- Martin P1, Dubois C1, Jacquier E1, Dion S2, Mancini-Bourgine M2, Godon O2, Kratzer R1, Lelu-Santolaria K1, Evlachev A1, Meritet JF3, Schlesinger Y4, Villeval D4, Strub JM5, Van Dorsselaer A5, Marchand JB4, Geist M4, Brandely R4, Findeli A4, Boukhebza H1, Menguy T4, Silvestre N4, Michel ML2, Inchauspé G1.
- Gut.Gut.2015 Dec;64(12):1961-71. doi: 10.1136/gutjnl-2014-308041. Epub 2014 Nov 26.
- OBJECTIVE: To assess a new adenovirus-based immunotherapy as a novel treatment approach to chronic hepatitis B (CHB).METHODS: TG1050 is a non-replicative adenovirus serotype 5 encoding a unique large fusion protein composed of a truncated HBV Core, a modified HBV Polymerase and two HBV Envelope doma
- PMID 25429051
Japanese Journal
- アダリムマブ投与中にニューモシスチス肺炎を発症した関節リウマチ患者2例の報告
- 池内 秀和,梅元 あずさ,月田 真祐子,櫻井 則之,前嶋 明人,黒岩 卓,廣村 桂樹,野島 美久
- 日本臨床免疫学会会誌 = Japanese journal of clinical immunology 34(5), 420-425, 2011-10-31
- … 症例とも胸部CTで間質性陰影を認め，β-D-グルカン高値であり，PCPと診断した．入院同日よりスルファメトキサゾール/トリメトプリム合剤を開始，70 Torr以下の低酸素血症があり高用量のPSLも併用した．症状は改善し，それぞれ第8，第16病日に退院した．喀痰Pneumocystis jirovecii DNAは両症例で陽性であり，β-D-グルカンと共に早期診断に有用であった．早期にST合剤を開始できたことも良好な予後につながったと考えられた． …
- NAID 10029898181
- Molecular identification of novel alpha- and gammaherpesviruses from cetaceans stranded on Japanese coasts
- Miyoshi Kanna,Nishida Shin,Sone Emi [他]
- Zoological science 28(2), 126-133, 2011-02
- NAID 40018702777
- 3.ALPHA.,23-Isopropylidenedioxyolean-12-en-27-oic Acid, a Triterpene Isolated from Aceriphyllum rossii, Induces Apoptosis in Human Cervical Cancer HeLa Cells through Mitochondrial Dysfunction and Endoplasmic Reticulum Stress
- Won So-Jung,Ki Yo Sook,Chung Kyung-Sook [他],Choi Jung-Hye,Bae Ki Hwan,Lee Kyung-Tae
- Biological and Pharmaceutical Bulletin 33(9), 1620-1626, 2010
- … Treatment with IPA significantly increased externalization of phosphatidylserine residues and apoptotic DNA fragmentation as shown by Annexin V staining and 4′,6-diamidino-2-phenylindole-dihydrochloride (DAPI) staining, respectively. … In addition, IPA induced the activations of caspase-8, -9, -3, and cleavage of poly(ADP ribose) polymerase (PARP-1) in HeLa cells. …
- NAID 130000322367
Related Links
- DNA polymerase alpha Heterotetrameric polymerase which has intrinsic DNA primase activity. Required for initial DNA synthesis of leading strand at origins and for priming of Okazaki ...
- Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase ...
★リンクテーブル★
[★]
- 関
- DNA polymerase alpha、DNA polymerase I、Klenow fragment、RNA polymerase I
[★]
クレノウ断片、クレノー断片、Klenow断片
- 関
- DNA polymerase alpha、DNA polymerase I、Pol I
[★]
[★]
- 英
- deoxyribonucleic acid
- 同
- デオキシリボ核酸
- 関
- リボ核酸 RNA
[★]
α、アルファ
- 関
- alfa