WordNet

a way of doing something, especially a systematic way; implies an orderly logical arrangement (usually in steps)
a family of compounds derived from horseradish and radishes and onions and mustards; source of the hotness of those plants and preparations

PrepTutorEJDIC

〈C〉(特に秩序だった)(…の)『方法』,方式《+『of』+『名』(do『ing』)》 / 〈C〉〈U〉(思考行・行為・行動の)きちょうめんさ;秩序,筋道

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1. 乳幼児および思春期前の小児における通常の成長パターン normal growth patterns in infants and prepubertal children
2. 在胎月齢の生後評価 postnatal assessment of gestational age
3. Fertility awareness法での避妊 fertility awareness based methods of pregnancy prevention
4. 低身長の小児や青年への診断的アプローチ diagnostic approach to children and adolescents with short stature
5. ハーブ剤および栄養補助食品による肝毒性 hepatotoxicity due to herbal medications and dietary supplements

English Journal

New method of peptide cleavage based on Edman degradation.

Bąchor R, Kluczyk A, Stefanowicz P, Szewczuk Z.Author information Faculty of Chemistry, University of Wrocław, ul. F. Joliot-Curie 14, 50-383 , Wrocław, Poland.AbstractA straightforward cleavage method for N- acylated peptides based on the phenylthiohydantoin (PTH) formation is presented. The procedure could be applied to acid-stable resins, such as TentaGel HL-NH[Formula: see text]. We designed a cleavable linker that consists of a lysine residue with the [Formula: see text]-amino group blocked by Boc, whereas the [Formula: see text]-amino group is used for peptide synthesis. After the peptide assembly is completed, the protecting groups in peptide side chains are removed using trifluoroacetic acid, thus liberating also the [Formula: see text]-amino group of the lysine in the linker. Then the reaction with phenyl isothiocyanate followed by acidolysis causes an efficient peptide release from the resin as a stable PTH derivative. Furthermore, the application of a fixed charge tag in the form of 2-(4-aza-1-azoniabicyclo[2.2.2]octylammonium)acetyl group increases ionization efficiency and reduces the detection limit, allowing ESI-MS/MS sequencing of peptides in the subfemtomolar range. The proposed strategy is compatible with standard conditions during one-bead-one-compound peptide library synthesis. The applicability of the developed strategy in combinatorial chemistry was confirmed using a small training library of [Formula: see text]-chymotrypsin substrates.
Molecular diversity.Mol Divers.2013 Aug;17(3):605-11. doi: 10.1007/s11030-013-9453-y. Epub 2013 May 21.
A straightforward cleavage method for N- acylated peptides based on the phenylthiohydantoin (PTH) formation is presented. The procedure could be applied to acid-stable resins, such as TentaGel HL-NH[Formula: see text]. We designed a cleavable linker that consists of a lysine residue with the [Formul
PMID 23690169

HI-bone: a scoring system for identifying phenylisothiocyanate-derivatized peptides based on precursor mass and high intensity fragment ions.

Perez-Riverol Y, Sánchez A, Noda J, Borges D, Carvalho PC, Wang R, Vizcaíno JA, Betancourt L, Ramos Y, Duarte G, Nogueira FC, González LJ, Padrón G, Tabb DL, Hermjakob H, Domont GB, Besada V.Author information Department of Proteomics, Center for Genetic Engineering and Biotechnology, Cubanacán, Playa, Ciudad de la Habana, Cuba.AbstractPeptide sequence matching algorithms used for peptide identification by tandem mass spectrometry (MS/MS) enumerate theoretical peptides from the database, predict their fragment ions, and match them to the experimental MS/MS spectra. Here, we present an approach for scoring MS/MS identifications based on the high mass accuracy matching of precursor ions, the identification of a high intensity b1 fragment ion, and partial sequence tags from phenylthiocarbamoyl-derivatized peptides. This derivatization process boosts the b1 fragment ion signal, which turns it into a powerful feature for peptide identification. We demonstrate the effectiveness of our scoring system by implementing it on a computational tool called "HI-bone" and by identifying mass spectra of an Escherichia coli sample acquired on an Orbitrap Velos instrument using Higher-energy C-trap dissociation. Following this strategy, we identified 1614 peptide spectrum matches with a peptide false discovery rate (FDR) below 1%. These results were significantly higher than those from Mascot and SEQUEST using a similar FDR.
Analytical chemistry.Anal Chem.2013 Apr 2;85(7):3515-20. doi: 10.1021/ac303239g. Epub 2013 Mar 20.
Peptide sequence matching algorithms used for peptide identification by tandem mass spectrometry (MS/MS) enumerate theoretical peptides from the database, predict their fragment ions, and match them to the experimental MS/MS spectra. Here, we present an approach for scoring MS/MS identifications bas
PMID 23448308

Japanese Journal

組換えヒト血清アルブミンの構造・組成分析

藥學雜誌 117(4), 220-232, 1997-04-25
NAID 10016243280

Improved method for identification and determination of ε-(γ-glutamyl)-lysine crosslink in protein using proteolytic digestion and derivatization with phenyl isothiocyanate followed by high-performance liquid chromatography separation