- 関
- electrofocusing、IEF、isoelectric focusing
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/02/15 01:24:05」(JST)
[Wiki en表示]
Chromatofocusing is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture according to differences in their isoelectric point. Chromatofocusing utilizes ion exchange resins and is typically performed on fast protein liquid chromatography (FPLC) or similar equipment capable of producing continuous buffer gradients though this is not a requirement. In contrast to typical ion exchange chromatography, where bound molecules are eluted from the resin by increasing the ionic strength of the buffer environment, chromatofocusing elutes bound species by altering the pH of the buffer. This changes the net surface charge of bound molecules, altering their avidity for the resin. As the changing pH of the buffer system traverses the pI of a given molecule, that molecule will elute from the resin as it will no longer possess a net surface charge (a requisite for molecular binding to ion exchange resins). Chromatofocusing is a powerful purification technique with respect to proteins as it can resolve very similar species only differing by 0.02 pH units that may not separate well, or at all, using traditional ion exchange strategies. A major drawback to this technique is that some proteins will aggregate when they are present at relatively high concentrations and carry no net surface charge. This can cause blockage of the resin, which is highly problematic when using sealed columns of ion exchange resin on FPLC equipment, resulting in pressure buildup and possible equipment failure. Apparent aggregation issues can sometimes be overcome by limiting the sample concentration and use of buffer additives that deter aggregate formation.
English Journal
- Purification and Characterization of a Fucoidanase (FNase S) from a Marine Bacterium Sphingomonas paucimobilis PF-1.
- Kim WJ1,2, Park JW3, Park JK4, Choi DJ5, Park YI6.
- Marine drugs.Mar Drugs.2015 Jul 16;13(7):4398-417. doi: 10.3390/md13074398.
- The Search for enzyme activities that efficiently degrade marine polysaccharides is becoming an increasingly important area for both structural analysis and production of lower-molecular weight oligosaccharides. In this study, an endo-acting fucoidanase that degrades Miyeokgui fucoidan (MF), a sulfa
- PMID 26193285
- Displacement chromatography of proteins using a retained pH front in a hydrophobic charge induction chromatography column.
- Pinto ND1, Frey DD2.
- Journal of chromatography. A.J Chromatogr A.2015 Mar 27;1387:53-9. doi: 10.1016/j.chroma.2015.01.087. Epub 2015 Feb 7.
- The chromatographic separation of two proteins into a displacement train of two adjoined rectangular bands was accomplished using a novel method for hydrophobic charge induction chromatography (HCIC) which employs a self-sharpening pH front as the displacer. This method exploits the fact that protei
- PMID 25702080
- Purification of Δ(5)-3-ketosteroid isomerase from Digitalis lanata.
- Meitinger N1, Geiger D2, Augusto TW2, Maia de Pádua R3, Kreis W2.
- Phytochemistry.Phytochemistry.2015 Jan;109:6-13. doi: 10.1016/j.phytochem.2014.10.025. Epub 2014 Nov 14.
- The isomerization of 5-pregnene-3,20-dione into 4-pregnene-3,20-dione was investigated to shed further light on cardenolide biosynthesis and to characterize the enzymes involved in cardenolide formation. It was shown that the Δ(5)-3-ketosteroid isomerase of Digitalis lanata, which catalyzes this is
- PMID 25468533
Japanese Journal
- Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive Methylenediphosphonic Acid (Mass Spectrometry)
- Journal of the Mass Spectrometry Society of Japan = 質量分析 62(6), 1-9, 2014-12
- NAID 40020288141
- Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive Methylenediphosphonic Acid
- Reducing sample complexity of polyclonal human autoantibodies by chromatofocusing
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 878(24), 2249-2254, 2010-08-15
- NAID 10028040330
Related Links
- Chromatofocusing is a protein-separation technique that was introduced by Sluyterman and his colleagues between 1977 and 1981 (1 – 5). Chromatofocusing combines the advantage of high-capacity ion-exchange procedures with the ...
- Separate your proteins based on isoelectric point using our chromatofocusing columns, media, and buffers ... We offer several products for chromatofocusing. This includes Mono P column 5/50 for rapid scouting of elution conditions ...
★リンクテーブル★
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- 英
- isoelectric focusing、IEF、chromatofocusing、electrofocusing
- 関
- クロマトフォーカシング、等電点分離法
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- 関
- chromatofocusing、electrofocusing、IEF
- 同
- IEF＆antibody heterogeneity
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- 関
- chromatofocusing、IEF、isoelectric focusing
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- 関
- chromatofocusing、electrofocusing、isoelectric focusing
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- 英
- chromatofocusing
- 関
- 等電点電気泳動