WordNet

the two innermost layers of the meninges; cerebrospinal fluid circulates between these innermost layers
put a coat on; cover the surface of; furnish with a surface; "coat the cake with chocolate" (同)surface
cover or provide with a coat
growth of hair or wool or fur covering the body of an animal (同)pelage
an outer garment that has sleeves and covers the body from shoulder down; worn outdoors
form a coat over; "Dirt had coated her face" (同)cake
of the yellowish-beige color of buff leather
a soft thick undyed leather from the skins of e.g. buffalo or oxen
an implement consisting of soft material mounted on a block; used for polishing (as in manicuring) (同)buffer
bare skin; naked; "swimming in the buff"
polish and make shiny; "buff the wooden floors"; "buff my shoes" (同)burnish, furbish
a thin layer covering something; "a second coat of paint" (同)coat
a decorative texture or appearance of a surface (or the substance that gives it that appearance); "the boat had a metallic finish"; "he applied a coat of a clear finish"; "when the finish is too thin it is difficult to apply evenly" (同)finish, finishing
a heavy fabric suitable for coats
having or dressed in a coat
having a coating; covered with an outer layer or film; often used in combination; "coated paper has a smooth polished coating especially suitable for halftone printing"; "sugar-coated pills"

PrepTutorEJDIC

『コート』,オーバー,外とう / (男子背広・婦人スーツの)『上着』;(婦人・子供の)長上着 / (動物の)毛,毛皮;(植物の)外被,皮 / 表面をおおうもの;(ペンキ・ニスなどの)塗り;(金属などの)めっき / 〈人〉‘に'上旦を旦せる / 〈ほこりなどが〉〈症など〉'を'『おおう』;(ペンキなどで)〈壁など〉'を'塗る《+『名』+『with』+『名』》
〈U〉もみ皮(牛・水牛などから採った黄褐色の皮;レンズなどをみがくのに用いる) / 〈U〉黄褐色 / 〈U〉(物をみがくのに用いる皮を張った)とぎ革 / 〈U〉《話》《おもに英》素肌(すはだ) / 〈C〉《話》ファン,…狂(fan) / もみ皮製の / 黄褐色の / 〈金属〉'を'もみ皮でみがく
〈C〉(ペンキなどの)上塗り,塗装;(食物の)ころも,皮 / 〈U〉上旦用の布地

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/08/05 10:00:43」(JST)

wiki en

Blood components after centrifugation.

Human blood fractionated by centrifugation. Plasma (upper layer), buffy coat (middle, white coloured layer) and erythrocyte (red blood cell) layer (bottom) can be seen.

The buffy coat is the fraction of an anticoagulated blood sample that contains most of the white blood cells and platelets following density gradient centrifugation of the blood.

Description

After centrifugation, one can distinguish a layer of clear fluid (the plasma), a layer of red fluid containing most of the red blood cells, and a thin layer in between. Making up less than 1% of the total volume of the blood sample, the buffy coat (so-called because it is usually buff in hue), contains most of the white blood cells and platelets. The buffy coat is used, for example, to extract DNA from the blood of mammals (since mammalian red blood cells are anucleate and do not contain DNA).

The buffy coat is usually whitish in color, but is sometimes green if the blood sample contains large amounts of neutrophils (which are high in green myeloperoxidase). The layer beneath the buffy coat contains granulocytes and red blood cells.

Preparation

The following is a standard protocol for preparing a buffy coat from whole human blood.^[1] Biohazard precautions for working with potentially infectious material such as human body fluids should be strictly adhered to.

Collect whole blood in tube containing an anticoagulant such as sodium heparin.
Dilute fresh blood using an equal volume of calcium- and magnesium-free PBS with 2% v/v FBS.
Centrifuge at 200 x g for 10 minutes at room temperature.
Using a pipette, carefully remove the concentrated white blood cells at the plasma/erythrocyte interface. Higher numbers of cells can be collected by removing some of the serum and red blood cells, which can be lysed later if necessary.

Diagnostic uses of the buffy coat

Quantitative buffy coat (QBC) is a laboratory test to detect infection with malaria or other blood parasites. The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the fluorescing parasites can then be observed under ultraviolet light at the interface between red blood cells and buffy coat. This test is more sensitive than the conventional thick smear and in > 90% of cases the species of parasite can also be identified.
In cases of extremely low white blood cell count, it may be difficult to perform a manual differential of the various types of white cells, and it may be virtually impossible to obtain an automated differential. In such cases the medical technologist may obtain a buffy coat, from which a blood smear is made. This smear contains a much higher number of white blood cells than whole blood.

References

^ "Human Immunology Portal - Preparing a Buffy Coat from Whole Blood". Retrieved 1 July 2014.

Marieb, Elaine N. (2007). Human Anatomy & Physiology (Seventh Edition ed.). San Francisco: Pearson Benjamin Cummings. ISBN 0-8053-5910-9.

UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

1. 血液成分の調製 preparation of blood components
2. ヒトエーリキア症およびアナプラズマ症 human ehrlichiosis and anaplasmosis
3. Rh（D）同種免疫の予防 prevention of rh d alloimmunization
4. 血小板輸血療法の臨床上および検査上の特徴 clinical and laboratory aspects of platelet transfusion therapy
5. 成人における24時間携帯血圧測定および白衣高血圧症 ambulatory blood pressure monitoring and white coat hypertension in adults

English Journal

Azithromycin suppresses human osteoclast formation and activity in vitro.

Gannon SC, Cantley MD, Haynes DR, Hirsch R, Bartold PM.SourceColgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, South Australia, Australia.
Journal of cellular physiology.J Cell Physiol.2013 May;228(5):1098-107. doi: 10.1002/jcp.24259.
Azithromycin is an antibiotic with anti-inflammatory properties used as an adjunct to treat periodontitis, a common inflammatory mediated condition featuring pathologic alveolar bone resorption. This study aimed to determine the effect of azithromycin on human osteoclast formation and resorptive act
PMID 23065774

Detection of cytokeratin-20-positive cells in preoperative and postoperative blood samples from colorectal cancer patients by real-time RT-PCR.

Samija I, Lukac J, Mubrin MK, Kirac I, Kovacevic D, Kusic Z, Mubrin MK, Kovac Evic D.SourceDepartment of Oncology and Nuclear Medicine, University Hospital Center "Sestre milosrdnice", Zagreb - Croatia.
The International journal of biological markers.Int J Biol Markers.2013 Apr 5:0. doi: 10.5301/jbm.5000003. [Epub ahead of print]
Background: Detection of circulating cancer cells by reverse transcription polymerase chain reaction (RT-PCR) has been studied as a prognostic marker in patients with colorectal cancer (CRC) but so far with conflicting results regarding specificity and prognostic value. In this study cytokeratin-20
PMID 23558939

Japanese Journal

S状結腸癌による髄膜癌腫症の1例

日本臨床外科学会雑誌 = The journal of the Japan Surgical Association 74(1), 124-128, 2013-01-25
NAID 10031165540

Short (GT)n Microsatellite Repeats in the Heme Oxygenase-1 Gene Promoter Are Associated with Antioxidant and Anti-Inflammatory Status in Mexican Pediatric Patients with Sepsis

The Tohoku Journal of Experimental Medicine 231(3), 201-209, 2013
NAID 130004720756

Quantitation and Human Monocyte Cytotoxicity of the Polymerization Agent 1-Hydroxycyclohexyl Phenyl Ketone (Irgacure 184) from Three Brands of Aqueous Injection Solution

Biological & Pharmaceutical Bulletin 35(10), 1821-1825, 2012-10
NAID 120005468279

Related Pictures

★リンクテーブル★

リンク元	「バフィコート」「leptomeningeal」「leptomeninges」「pial」
関連記事	「coat」「coated」「coating」

「バフィコート」

　　[★]

英: buffy coat
同: バフィーコート
関: 軟膜

[show details]

バフィコート : 約 73 件
バッフィコート : 約 33 件

「leptomeningeal」

　　[★]

adj.

軟髄膜の、軟膜の

関: buffy coat、leptomeninges、pia mater、pial

「leptomeninges」

　　[★]

n.

軟膜、軟髄膜

関: buffy coat、leptomeningeal、pia mater、pial

「pial」

　　[★]

adj.

軟膜の

関: buffy coat、leptomeningeal、leptomeninges、pia mater

「coat」

　　[★]

n.

コート、被膜、外被

v.

コーティングする

関: coating、crust、envelope、integumental、integumentary、investment、tegument、tunicate

「coated」

　　[★]

被覆の

関: blanket、coverage、covering

「coating」

　　[★]

コーティング

関: coat

[1] "Human Immunology Portal - Preparing a Buffy Coat from Whole Blood". Retrieved 1 July 2014.

匿名

検索

案内

案内

buffy coat