- 関
- BHK cell
WordNet
- a project of personal concern to someone; "this project is his baby"
- a very young mammal; "baby rabbits"
- a very young child (birth to 1 year) who has not yet begun to walk or talk; "the baby began to cry again"; "she held the baby in her arms"; "it sounds simple, but when you have your own baby it is all so different" (同)babe, infant
- (slang) sometimes used as a term of address for attractive young women (同)babe, sister
- an unborn child; a human fetus; "I felt healthy and very feminine carrying the baby"; "it was great to feel my baby moving about inside"
- the youngest member of a group (not necessarily young); "the baby of the family"; "the baby of the Supreme Court"
- small room in which a monk or nun lives (同)cubicle
- a device that delivers an electric current as the result of a chemical reaction (同)electric cell
- a room where a prisoner is kept (同)jail cell, prison cell
- (biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals
- any small compartment; "the cells of a honeycomb"
- a small unit serving as part of or as the nucleus of a larger political movement (同)cadre
- either of two bean-shaped excretory organs that filter wastes (especially urea) from the blood and excrete them and water in urine; "urine passes out of the kidney through ureters to the bladder"
- short-tailed Old World burrowing rodent with large cheek pouches
PrepTutorEJDIC
- 『赤ん坊,赤ちゃん』 / (家族・グループの中で)いちばん年少の人,最年少者 / 赤ん坊みたいな人 / (また『babe』)《米俗》《特に若い女の子への呼びかけた用いて》かわいこちゃん / 赤ん坊の[ような],赤ちゃん用の / 《話》…'を'赤ちゃんのように扱う,甘やかす
- (刑務所の)『独房』;(修道院の)小さい独居室 / (ミツバチの)みつ房,巣穴 / 小さい部屋 / 『細胞』 / 電池 / 花粉室 / (共産党などの)細胞
- 腎臓;(食品としての)羊(豚など)の腎臓 / 《文》気質,性質,たち(nature)
- ハムスター,チョウセンネズミ
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2014/05/16 20:19:20」(JST)
[Wiki en表示]
Baby Hamster Kidney fibroblasts (aka BHK cells) are an adhesive cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays occasionally used, subclone 13 which originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.
Contents
- 1 BHK and virus infection
- 2 Utilization
- 3 Recommended growth medium
- 4 References
BHK and virus infection
BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain) BHK-21 cells are resistant to poliovirus 2. the cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes.
Utilization
The BHK-21 cells are useful for transformations and for stable and temporary transfections.
Recommended growth medium
high- glucose DMEM FBS fetal bovine serum 5% (when you freeze, add 10%) GLU glutamine 1% PSA regular antibiotics 1%
Splitting :10 by Trypsin[1][2]
References
- ^ http://www.olympusmicro.com/primer/techniques/fluorescence/gallery/cells/bhk/bhkcells.html
- ^ תוצאות חיפוש תמונות ב-Google עבור http://www.clinical-virology.org/gallery/images/cpes/uninfected_BHK21_cells.jpg
UpToDate Contents
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English Journal
- Gene expression profiling for mechanistic understanding of cellular aggregation in mammalian cell perfusion cultures.
- Liu M, Goudar CT.SourceCell Culture Development, Global Biological Development, Bayer HealthCare, 800 Dwight Way, Berkeley, California 94710; telephone: 805-410-2005; fax: 805-447-1010.
- Biotechnology and bioengineering.Biotechnol Bioeng.2013 Feb;110(2):483-90. doi: 10.1002/bit.24730. Epub 2012 Oct 18.
- Aggregation of baby hamster kidney (BHK) cells cultivated in perfusion mode for manufacturing recombinant proteins was characterized. The potential impact of cultivation time on cell aggregation for an aggregating culture (cell line A) was studied by comparing expression profiles of 84 genes in the
- PMID 23007466
- Topical photodynamic therapy using transfersomal aluminum phthalocyanine tetrasulfonate: in vitro and in vivo study.
- Kassab K, El Fadeel DA, Fadel M.SourcePhotobiology and Cell Photosensitization Lab, Department of Laser Medical Applications, National Institute of Laser Enhanced Science, Cairo University, Cairo, Egypt.
- Lasers in medical science.Lasers Med Sci.2013 Jan 5. [Epub ahead of print]
- The efficacy of transfersomes (flexible liposomes) as a novel technique for topical delivery of the hydrophilic tetra-anionic photodynamic sensitizer aluminum (III) phthalocyanine tetrasulfonate (AlPcS4) was investigated, on mammalian fibroblasts and on Balb/c mice dorsal skin. AlPcS4 was loaded in
- PMID 23291878
Japanese Journal
- Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection
- Kim Eunmi,Okumura Megumi,Sawa Hirofumi,Miyazaki Tadaaki,Fujikura Daisuke,Yamada Shuhei,Sugahara Kazuyuki,Sasaki Michihito,Kimura Takashi
- Biochemical and Biophysical Research Communications 409(4), 717-722, 2011-06-17
- … Soluble CS-E (sCS-E) derived from squid cartilage inhibited JEV infection in African green monkey kidney-derived Vero cells and baby hamster kidney-derived BHK cells by interfering with viral attachment. … In contrast, sCS-E enhanced viral infection in the mouse neuroblastoma cell line Neuro-2a, despite the fact that viral attachment to Neuro-2a cells was inhibited by sCS-E. …
- NAID 80021801833
- Isolation of Trypanosoma (Megatrypanum) theileri from Dairy Cattle in Taiwan
- LEE Yen-Feng,CHENG Ching-Chang,LIN Nai-Nu,LIU Shih-An,TUNG Kwong-Chung,CHIU Yung-Tsung
- Journal of Veterinary Medical Science advpub(0), 0911280079, 2010
- … However, a highly distinctive morphological feature of trypanosome appeared in baby hamster kidney (BHK) cell culture inoculated with non-thrown blood buffy coat. … theileri isolate in Taiwan (TWTth1) can be periodicaly passaged in BHK cell culture for more than one year and retain good re-cryopreservation viability. …
- NAID 130000149938
Related Links
- BHK cell BHK cell Baby hamster kidney cell. An established diploid cell line descended from a clone (Clone 13) isolated by Stoker and McPherson from a rapidly growing primary culture of newborn Syrian hamster kidney tissue. BHK ...
- Acronym Definition BHKC British Hand Knitting Confederation (UK) BHKC Baby Hamster Kidney Cell (biology)
Related Pictures
★リンクテーブル★
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- 関
- baby hamster kidney cell
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- 英
- baby hamster kidney cell、BHK cell
- 関
- BHK細胞
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- 関
- infant、infantile、newborn infant、suckling、sucklings
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ハムスター
- 関
- Chinese hamster、Cricetinae、Cricetulus
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細胞
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- 関
- renal cell
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腎臓
- 同
- KUB