同: cleavage and polyadenylation specificity factor

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/05/23 11:00:21」(JST)

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Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. It is the first protein to bind to the signaling region near the cleavage site of the pre-mRNA, to which the poly(A) tail will be added by polynucleotide adenylyltransferase. The upstream signaling region has the canonical nucleotide sequence AAUAAA, which is highly conserved across the vast majority of pre-mRNAs. A second downstream signaling region, located on the portion of the pre-mRNA that is cleaved before polyadenylation, consists of a GU-rich region required for efficient processing.

Structure

CPSF is a protein complex consisting of four proteins: CPSF-73, CPSF-100, CPSF-30 and CPSF-160.

CPSF-73 is a zinc-dependent hydrolase which cleaves the mRNA precursor just downstream the polyadenylation signal sequence AAUAAA.^[1]

CPSF-160 is the largest subunit of CPSF and directly binds to the AAUAAA polyadenylation signal.^[2]

CPSF recruits additional proteins to the 3' region. Identified proteins that are coordinated by CPSF activity include: cleavage stimulatory factor and the two poorly understood cleavage factors. The binding of the polynucleotide adenylyltransferase responsible for actually synthesizing the tail is a necessary prerequisite for cleavage, thus ensuring that cleavage and polyadenylation are tightly coupled processes.

Genes

CPSF1, CPSF2, CPSF3, CPSF4, CPSF6

References

^ Mandel, Corey R.; Kaneko, Syuzo; Zhang, Hailong; Gebauer, Damara; Vethantham, Vasupradha; Manley, James L.; Tong, Liang (26 November 2006). "Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease". Nature 444 (7121): 953–956. doi:10.1038/nature05363. PMID 17128255. Retrieved 17 December 2014.
^ Murthy, Kanneganti G K; Manley, James L (1 November 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes & Development 9 (21): 2672–2683. doi:10.1101/gad.9.21.2672. PMID 7590244. Retrieved 17 December 2014.

Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott MP, Zipursky SL, Darnell J. (2004). Molecular Cell Biology. WH Freeman: New York, NY. 5th ed.
Murthy, KG; Manley, JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Gene Dev 9: 2672–2683. doi:10.1101/gad.9.21.2672. PMID 7590244.

External links

Cleavage And Polyadenylation Specificity Factor at the US National Library of Medicine Medical Subject Headings (MeSH)

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English Journal

[Preliminary experiences of endoscopic CO2 laser cauterization for treatment of congenital pyriform sinus fistula].

Chen L1, Liang L2, Luo X2, Guo M2, Zhang S2, Lu Z2, Xu M2.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery.Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi.2014 Jul;49(7):582-5.
OBJECTIVE: To evaluate the effectiveness of endoscopic CO2 laser cauterization (ECLC) as a definitive treatment of congenital pyriform sinus fistula (CPSF).METHODS: Eleven patients with CPSF underwent ECLC between January 2011 to March 2013 at Guangdong General Hospital.Of the 11 patients aging from
PMID 25257274

Pharmacists' influences on prescribing: Validating a clinical pharmacy services survey in the Western Pacific Region.

Penm J1, Chaar B2, Rose G3, Moles R2.
Research in social & administrative pharmacy : RSAP.Res Social Adm Pharm.2014 Jun 19. pii: S1551-7411(14)00037-0. doi: 10.1016/j.sapharm.2014.04.001. [Epub ahead of print]
BACKGROUND: Hospital pharmacists around the world are becoming increasingly involved in promoting the responsible use of medicines through clinical pharmacy services. This is reflected in the Basel Statements developed by the International Pharmaceutical Federation Hospital Pharmacy Section, particu
PMID 25042568

3'-End processing of histone pre-mRNAs in Drosophila: U7 snRNP is associated with FLASH and polyadenylation factors.

Sabath I, Skrajna A, Yang XC, Dadlez M, Marzluff WF, Dominski Z.
RNA (New York, N.Y.).RNA.2013 Dec;19(12):1726-44. doi: 10.1261/rna.040360.113. Epub 2013 Oct 21.
3'-End cleavage of animal replication-dependent histone pre-mRNAs is controlled by the U7 snRNP. Lsm11, the largest component of the U7-specific Sm ring, interacts with FLASH, and in mammalian nuclear extracts these two proteins form a platform that recruits the CPSF73 endonuclease and other polyade
PMID 24145821