Protein expression systems are widely used in biotechnology and medicine for the efficient and economic production of therapeutic proteins. Today, cultivated Chinese hamster ovary (CHO) cells are the market dominating mammalian cell-line for the production of complex therapeutic proteins. Despite th
Role of cysteine-protease CGHC motifs of ER-60, a protein disulfide isomerase, in hepatic apolipoprotein B100 degradation.
Rutledge AC1, Qiu W, Zhang R, Urade R, Adeli K.
Archives of biochemistry and biophysics.Arch Biochem Biophys.2013 Sep 1;537(1):104-12. doi: 10.1016/j.abb.2013.06.013. Epub 2013 Jul 1.
Apolipoprotein B100 (apoB), the structural component of very low density lipoproteins (VLDL), is susceptible to misfolding and subsequent degradation by several intracellular pathways. ER-60, which has been implicated in apoB degradation, is a protein disulfide isomerase (PDI) that forms or rearrang
Cell-free systems: functional modules for synthetic and chemical biology.
Stech M1, Brödel AK, Quast RB, Sachse R, Kubick S.
Advances in biochemical engineering/biotechnology.Adv Biochem Eng Biotechnol.2013;137:67-102. doi: 10.1007/10_2013_185.
: The main goal of cell-free protein synthesis is to produce correctly folded and functional proteins in reasonable amounts for further downstream applications. Especially for eukaryotic proteins, functionality is often directly linked to the presence of posttranslational modifications. Thus, it is