WordNet

a substance from which another substance is formed (especially by a metabolic reaction)
a person who goes before or announces the coming of another (同)forerunner

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Quantitative evaluation of alternatively spliced mRNA isoforms by label-free real-time plasmonic sensing.

Huertas CS1, Carrascosa LG1, Bonnal S2, Valcárcel J3, Lechuga LM4.
Biosensors & bioelectronics.Biosens Bioelectron.2016 Apr 15;78:118-25. doi: 10.1016/j.bios.2015.11.023. Epub 2015 Nov 10.
Alternative splicing of mRNA precursors enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status. Its deregulation is strongly linked to disease onset and progression. Current methodologies for monitoring alternative splicing deman
PMID 26599481

Therapeutic antidepressant potential of a conjugated siRNA silencing the serotonin transporter after intranasal administration.

Ferrés-Coy A1,2,3, Galofré M1,2,3, Pilar-Cuéllar F3,4, Vidal R3,4, Paz V1,2,3, Ruiz-Bronchal E1,2,3, Campa L1,2,3, Pazos Á3,4, Caso JR3,5, Leza JC3,5, Alvarado G6, Montefeltro A6, Valdizán EM3,4, Artigas F1,2,3, Bortolozzi A1,2,3.
Molecular psychiatry.Mol Psychiatry.2016 Mar;21(3):328-38. doi: 10.1038/mp.2015.80. Epub 2015 Jun 23.
Major depression brings about a heavy socio-economic burden worldwide due to its high prevalence and the low efficacy of antidepressant drugs, mostly inhibiting the serotonin transporter (SERT). As a result, ~80% of patients show recurrent or chronic depression, resulting in a poor quality of life a
PMID 26100539

Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons.

Krishnaswami SR1, Grindberg RV2, Novotny M1, Venepally P3, Lacar B4, Bhutani K1, Linker SB4, Pham S4, Erwin JA4, Miller JA5, Hodge R5, McCarthy JK1, Kelder M4, McCorrison J1, Aevermann BD1, Fuertes FD1,6, Scheuermann RH1, Lee J7, Lein ES5, Schork N1, McConnell MJ8, Gage FH4, Lasken RS1.
Nature protocols.Nat Protoc.2016 Mar;11(3):499-524. doi: 10.1038/nprot.2016.015. Epub 2016 Feb 18.
A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for cDNA synthesis and Nextera XT bar
PMID 26890679