サブトラクティブハイブリダイゼーション、差引きハイブリダイゼーション法
WordNet
- (genetics) the act of mixing different species or varieties of animals or plants and thus to produce hybrids (同)hybridisation, crossbreeding, crossing, cross, interbreeding, hybridizing
- constituting or involving subtraction; "a subtractive correction"
PrepTutorEJDIC
- 引き算の;控除の
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2012/11/06 21:48:07」(JST)
[Wiki en表示]
Subtractive hybridization is a technology that allows for PCR-based amplification of only cDNA fragments that differ between a control (driver) and experimental transcriptome. Differences in relative abundance of transcripts are highlighted, as are genetic differences between species. The technique relies on the removal of dsDNA formed by hybridization between a control and test sample, thus eliminating cDNAs or gDNAs of similar abundance, and retaining differentially expressed, or variable in sequence, transcripts or genomic sequences.
Suppression subtractive hybridization has also been successfully used to identify strain- or species-specific DNA sequences in a variety of bacteria including Vibrio species.
See also
- Representational Difference Analysis
External links
- Overview at evrogen.com
- Munir S, Singh S, Kaur K, Kapur V (2004). "Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells". Biol Proced Online 6: 94–104. doi:10.1251/bpo77. PMC 420231. PMID 15181476. //www.ncbi.nlm.nih.gov/pmc/articles/PMC420231/. link
- Diatchenko L, Lau Y, Campbell A, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E, Siebert P (1996). "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries". Proc Natl Acad Sci USA 93 (12): 6025–30. doi:10.1073/pnas.93.12.6025. PMC 39182. PMID 8650213. //www.ncbi.nlm.nih.gov/pmc/articles/PMC39182/.
- Galbraith E, Antonopoulos D, White B (2004). "Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome: the rumen as a model". Environ Microbiol 6 (9): 928–37. doi:10.1111/j.1462-2920.2004.00575.x. PMID 15305918.
UpToDate Contents
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English Journal
- Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis.
- Deng WW, Zhang M, Wu JQ, Jiang ZZ, Tang L, Li YY, Wei CL, Jiang CJ, Wan XC.SourceKey Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
- Journal of plant physiology.J Plant Physiol.2013 Feb 15;170(3):272-82. doi: 10.1016/j.jplph.2012.10.010. Epub 2012 Dec 8.
- Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) is considered to be a key enzyme in lignin biosynthesis, but little was known about CADs in tea plants (Camellia sinensis). A full-length cDNA sequence (CsCAD2) was isolated by suppressive subtractive hybridization (SSH) in Ectropis oblique feeding-
- PMID 23228629
- Identification of genes involved in indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.) by suppression subtractive hybridization.
- Wei K, Wang L, Cheng H, Zhang C, Ma C, Zhang L, Gong W, Wu L.SourceNational Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, P. R. China.
- Gene.Gene.2013 Feb 10;514(2):91-8. doi: 10.1016/j.gene.2012.11.008. Epub 2012 Nov 29.
- The plant hormone auxin plays a key role in adventitious rooting. To increase our understanding of genes involved in adventitious root formation, we identified transcripts differentially expressed in single nodal cuttings of Camellia sinensis treated with or without indole-3-butyric acid (IBA) by su
- PMID 23201417
Japanese Journal
- Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization
- Species identification of Chinese medicinal plant Fallopia multiflora (Thunb.) Haraldson by suppression subtraction hybridization
- Construction and Analysis of Subtractive cDNA Library of Muscular Tissue in Hybrid of Chicken-Quail Related to Myogenesis
Related Links
- Subtractive hybridization is a technology that allows for PCR-based amplification of only cDNA fragments that differ between a control (driver) and experimental transcriptome. Differences in relative abundance of transcripts are highlighted, ...
- Subtractive hybridization is a powerful technique to study gene expression in specific tissues or cell types or at a specific stage. Traditional procedures often are technically demanding and labour-intensive methods that require large amounts ...
Related Pictures
![Suppressive Subtractive Hybridization](https://1mg.info/0/3/h/0539.jpg)
![Restriction Site Associated Dna](https://1mg.info/0/3/h/0540.jpg)
![Suppression Subtractive Hybridization](https://1mg.info/0/3/h/0541.jpg)
![subtractive hybridization and rna](https://1mg.info/0/3/h/0542.jpg)
![of Suppression Subtractive Hybridization](https://1mg.info/0/3/h/0543.jpg)
★リンクテーブル★
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- 英
- subtractive hybridization
- 関
- サブトラクティブハイブリダイゼーション
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- 英
- subtractive hybridization
- 関
- 差引きハイブリダイゼーション法
[★]
- 関
- cross、crossbred、crossbreeding、crossing、hybrid、hybridisation、hybridize、interbreed
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- 関
- subtract、subtraction