放射線ハイブリッドマッピング
- 関
- radiation hybrid、radiation hybrid map
WordNet
- produced by crossbreeding (同)intercrossed
- (genetics) an organism that is the offspring of genetically dissimilar parents or stock; especially offspring produced by breeding plants or animals of different varieties or breeds or species; "a mule is a cross between a horse and a donkey" (同)crossbreed, cross
- a composite of mixed origin; "the vice-presidency is a hybrid of administrative and legislative offices"
- the spontaneous emission of a stream of particles or electromagnetic rays in nuclear decay (同)radioactivity
- energy that is radiated or transmitted in the form of rays or waves or particles
- the act of spreading outward from a central source
- a radial arrangement of nerve fibers connecting different parts of the brain
- the spread of a group of organisms into new habitats
- (genetics) the process of locating genes on a chromosome (同)chromosome_mapping
PrepTutorEJDIC
- (動物植の)雑種,(物の)混成物 / 混種語(語源の異なる二つ以上の要素から成る語;たとえばshortageは英語(short)とフランス語(hybridage)の混種語) / 雑種(混成)の
- 〈U〉(光・熱・放射能などの)発散,放射 / 〈C〉放射物;放射線;輻射(ふくしゃ)熱 / 〈C〉(喜び・幸福感などの)発散《+『of』+『名』》 / =radioactivity
- (数学で)関数 / 地図製作 / (時間の)割当て
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/12/09 06:36:35」(JST)
[Wiki en表示]
|
|
This article does not cite any references or sources. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. (February 2011) |
Radiation hybrid mapping (also known as RH mapping) is a technique for mapping mammalian chromosomes.
Radiation hybrid mapping uses X-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs.
The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosome.
UpToDate Contents
全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.
English Journal
- Stronger transferability but lower variability in transcriptomic- than in anonymous microsatellites: evidence from Hylid frogs.
- Dufresnes C, Brelsford A, Béziers P, Perrin N.Author information Department of Ecology & Evolution, University of Lausanne, Biophore Building, Lausanne , 1015, Switzerland.AbstractA simple way to quickly optimize microsatellites in nonmodel organisms is to reuse loci available in closely related taxa; however, this approach can be limited by the stochastic and low cross-amplification success experienced in some groups (e.g. amphibians). An efficient alternative is to develop loci from transcriptome sequences. Transcriptomic microsatellites have been found to vary in their levels of cross-species amplification and variability, but this has to date never been tested in amphibians. Here, we compare the patterns of cross-amplification and levels of polymorphism of 18 published anonymous microsatellites isolated from genomic DNA vs. 17 loci derived from a transcriptome, across nine species of tree frogs (Hyla arborea and Hyla cinerea group). We established a clear negative relationship between divergence time and amplification success, which was much steeper for anonymous than transcriptomic markers, with half-lives (time at which 50% of the markers still amplify) of 1.1 and 37 My, respectively. Transcriptomic markers are significantly less polymorphic than anonymous loci, but remain variable across diverged taxa. We conclude that the exploitation of amphibian transcriptomes for developing microsatellites seems an optimal approach for multispecies surveys (e.g. analyses of hybrid zones, comparative linkage mapping), whereas anonymous microsatellites may be more informative for fine-scale analyses of intraspecific variation. Moreover, our results confirm the pattern that microsatellite cross-amplification is greatly variable among amphibians and should be assessed independently within target lineages. Finally, we provide a bank of microsatellites for Palaearctic tree frogs (so far only available for H. arborea), which will be useful for conservation and evolutionary studies in this radiation.
- Molecular ecology resources.Mol Ecol Resour.2013 Dec 17. doi: 10.1111/1755-0998.12215. [Epub ahead of print]
- A simple way to quickly optimize microsatellites in nonmodel organisms is to reuse loci available in closely related taxa; however, this approach can be limited by the stochastic and low cross-amplification success experienced in some groups (e.g. amphibians). An efficient alternative is to develop
- PMID 24345298
- Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat.
- Bassi FM, Kumar A, Zhang Q, Paux E, Huttner E, Kilian A, Dizon R, Feuillet C, Xu SS, Kianian SF.Author information Department of Plant Sciences, North Dakota State University, Fargo, ND 58102, USA. filippo.bassi@ndsu.eduAbstractSince the dawn of wheat cytogenetics, chromosome 3B has been known to harbor a gene(s) that, when removed, causes chromosome desynapsis and gametic sterility. The lack of natural genetic diversity for this gene(s) has prevented any attempt to fine map and further characterize it. Here, gamma radiation treatment was used to create artificial diversity for this locus. A total of 696 radiation hybrid lines were genotyped with a custom mini array of 140 DArT markers, selected to evenly span the whole 3B chromosome. The resulting map spanned 2,852 centi Ray with a calculated resolution of 0.384 Mb. Phenotyping for the occurrence of meiotic desynapsis was conducted by measuring the level of gametic sterility as seeds produced per spikelet and pollen viability at booting. Composite interval mapping revealed a single QTL with LOD of 16.2 and r (2) of 25.6 % between markers wmc326 and wPt-8983 on the long arm of chromosome 3B. By independent analysis, the location of the QTL was confirmed to be within the deletion bin 3BL7-0.63-1.00 and to correspond to a single gene located ~1.4 Mb away from wPt-8983. The meiotic behavior of lines lacking this gene was characterized cytogenetically to reveal striking similarities with mutants for the dy locus, located on the syntenic chromosome 3 of maize. This represents the first example to date of employing radiation hybrids for QTL analysis. The success achieved by this approach provides an ideal starting point for the final cloning of this interesting gene involved in meiosis of cereals.
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik.Theor Appl Genet.2013 Aug;126(8):1977-90. doi: 10.1007/s00122-013-2111-z. Epub 2013 May 29.
- Since the dawn of wheat cytogenetics, chromosome 3B has been known to harbor a gene(s) that, when removed, causes chromosome desynapsis and gametic sterility. The lack of natural genetic diversity for this gene(s) has prevented any attempt to fine map and further characterize it. Here, gamma radiati
- PMID 23715938
- A radiation hybrid map of chromosome 1D reveals synteny conservation at a wheat speciation locus.
- Michalak de Jimenez MK, Bassi FM, Ghavami F, Simons K, Dizon R, Seetan RI, Alnemer LM, Denton AM, Doğramacı M, Šimková H, Doležel J, Seth K, Luo MC, Dvorak J, Gu YQ, Kianian SF.Author information Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108-6050, USA.AbstractThe species cytoplasm specific (scs) genes affect nuclear-cytoplasmic interactions in interspecific hybrids. A radiation hybrid (RH) mapping population of 188 individuals was employed to refine the location of the scs (ae) locus on Triticum aestivum chromosome 1D. "Wheat Zapper," a comparative genomics tool, was used to predict synteny between wheat chromosome 1D, Oryza sativa, Brachypodium distachyon, and Sorghum bicolor. A total of 57 markers were developed based on synteny or literature and genotyped to produce a RH map spanning 205.2 cR. A test-cross methodology was devised for phenotyping of RH progenies, and through forward genetic, the scs (ae) locus was pinpointed to a 1.1 Mb-segment containing eight genes. Further, the high resolution provided by RH mapping, combined with chromosome-wise synteny analysis, located the ancestral point of fusion between the telomeric and centromeric repeats of two paleochromosomes that originated chromosome 1D. Also, it indicated that the centromere of this chromosome is likely the result of a neocentromerization event, rather than the conservation of an ancestral centromere as previously believed. Interestingly, location of scs locus in the vicinity of paleofusion is not associated with the expected disruption of synteny, but rather with a good degree of conservation across grass species. Indeed, these observations advocate the evolutionary importance of this locus as suggested by "Maan's scs hypothesis."
- Functional & integrative genomics.Funct Integr Genomics.2013 Mar;13(1):19-32. doi: 10.1007/s10142-013-0318-3. Epub 2013 Mar 12.
- The species cytoplasm specific (scs) genes affect nuclear-cytoplasmic interactions in interspecific hybrids. A radiation hybrid (RH) mapping population of 188 individuals was employed to refine the location of the scs (ae) locus on Triticum aestivum chromosome 1D. "Wheat Zapper," a comparative genom
- PMID 23479086
Japanese Journal
- Usefulness of CARTO 3 System in Ablating Paroxysmal Atrial Fibrillation
- Kimura Masaomi,Okumura Ken
- Journal of Arrhythmia 27(Supplement), MS3_1-MS3_1, 2011
- … Three-dimensional (3D) electroanatomical mapping system has been useful in identifying the critical area for the sustenance of tachycardias. … 3D mapping system also is useful in reducing the procedure time and the radiation exposure time during AF ablation. … This new system is characterized by a hybrid magnetic and current technology and enables exact localizations of Lasso catheter placed in the PV and ablation catheter. …
- NAID 130002130153
- SNP detection and radiation hybrid mapping in horses of nine candidate genes for temperament
- ウマのセロトニントランスポーター遺伝子の多型同定,RHマッピング,不安傾向との関連解析(短報)(動物行動学)
- 桃沢 幸秀,武内 ゆかり,戸崎 晃明,菊水 健史,長谷川 晃久,RAUDSEPP Terje,CHOWDHARY Bhanu P.,楠瀬 良,森 裕司
- The journal of veterinary medical science 68(6), 619-621, 2006-06-25
- 本研究ではウマの不安傾向に影響すると考えられるセロトニントランスポーター遺伝子について解析を行った.翻訳領域の配列は他の哺乳動物と高い相同性を示し,サラブレッド10頭の塩基配列を比較することで4つの多型が同定された.またRHマッピング法により同遺伝子が染色体11番上のneurofibromin 1遺伝子から26.92cRの地点に位置することが明らかとなった.さらに67頭のサラブレッドを用いて多型と …
- NAID 110004763164
Related Links
- Bibliography Cox, David R., Margit Burmeister, Price E. Roydon, Suwon Kim, and Richard M. Myers. "Radiation Hybrid Mapping: A Somatic Cell Genetic Method for Constructing High-Resolution Maps of Mammalian ...
- Radiation Hybrid Mapping Radiation hybrid mapping is a genetic technique that was originally developed for constructing long-range maps of mammalian chromosomes. It is based on a statistical method to determine not only the ...
★リンクテーブル★
[★]
- 英
- radiation hybrid mapping
- 関
- 放射線ハイブリッド、放射線ハイブリッドマッピング、放射線ハイブリッド地図
[★]
- 関
- radiation hybrid、radiation hybrid mapping
[★]
- 関
- radiation hybrid map、radiation hybrid mapping
[★]
- 英
- radiation hybrid mapping
- 関
- 放射線ハイブリッド地図作成
[★]
- 関
- bastard、chimera、complexed、crossbreeding、filial、hybridization、mongrel
[★]
- 関
- locate、map
[★]
- 関
- radiation hybrid map、radiation hybrid mapping
[★]