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Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus).

Baldassini WA1, Braga CP2, Chardulo LA1, Silva JA1, Malheiros JM3, de Albuquerque LG3, Fernandes TT4, Padilha Pde M4.
Food chemistry.Food Chem.2015 Feb 15;169:65-72. doi: 10.1016/j.foodchem.2014.07.131. Epub 2014 Aug 7.
The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence
PMID 25236199

Carbon storage in recombinant Escherichia coli during growth on glycerol and lactic acid.

Weiner M1, Tröndle J, Albermann C, Sprenger GA, Weuster-Botz D.
Biotechnology and bioengineering.Biotechnol Bioeng.2014 Dec;111(12):2508-19. doi: 10.1002/bit.25306. Epub 2014 Jul 29.
A fed-batch process was studied with lactate and glycerol supply in the growth phase and glycerol supply during L-phenylalanine production with recombinant E. coli K-12. Lactic acid feeding was necessary for growth because the genes encoding the PEP-consuming pyruvate kinase isoenzymes (pykA, pykF)
PMID 24902947

Metabolic Engineering of Acinetobacter baylyi ADP1 for Improved Growth on Gluconate and Glucose.

Kannisto M1, Aho T2, Karp M2, Santala V2.
Applied and environmental microbiology.Appl Environ Microbiol.2014 Nov 15;80(22):7021-7. doi: 10.1128/AEM.01837-14. Epub 2014 Sep 5.
A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi
PMID 25192990

諸杉健志,MOROSUGI Takeshi
法政大学大学院紀要（理工学・工学研究科編） (56), 2015-03-24
… The phosphoenolpyruvate (PEP)-dependent carbohydrate:phosphotransferase system (PTS), a major sugar uptake system widely distributed among bacteria, plays key roles in various aspects of signaling, including chemotaxis. … In chemotaxis, unphosphorylated EI inhibits the activity of the histidine kinase CheA, leading to an attractant response to the substrate sugar. …
NAID 120005616101

神内伸也,新藤由梨,内海有香,岩田直洋,岡﨑真理,鈴木史子,飯塚博,浅野哲,松崎広和,日比野康英
日本補完代替医療学会誌 11(1), 57-66, 2014-03
… Furthermore, the expression level of GLUT2 and activation of AMP kinase (AMPK) and glycogen synthase kinase 3β (GSk3β) was also determined by western blot analysis. … The gene expression and enzyme activities of both glucose-6-phosphatase and phosphoenolpyruvate carboxykinase were suppressed, whereas those of glucokinase were increased in the mice with WER intake and pioglitazone administration. …
NAID 120005520986

神内伸也,新藤由梨,内海有香,岩田直洋,岡真理,鈴木史子,飯塚博,浅野哲,松崎広和,日比野康英
日本補完代替医療学会誌 11(1), 57-66, 2014
【目的】霊芝菌糸体培養培地抽出物 (WER) の長期摂取による 2 型糖尿病マウスの血糖上昇抑制作用を明らかにするために，本研究では肝臓での糖質代謝について検討した．【方法】KK-Ay マウスに WER を混合した飼料を自由摂取させ，8 週間飼育した．飼育終了後の肝臓における糖代謝関連酵素の発現量および酵素活性を解析した．さらに，グリコーゲン量を測定するとともに，GLUT2 発現量および AMP …
NAID 130004461550

英: pyruvate kinase, PK
同: ホスホエノールピルビン酸キナーゼ phosphoenolpyruvate kinase、ホスホエノールトランスホスホリラーゼ phosphoenol transphosphorylase
関: 解糖系

CH₂-C(OPO₃^2-)-COOH + ADP + H⁺ －(ピルビン酸キナーゼ)→  CH₃-CO-COO^- + ATP

リンク元	「ピルビン酸キナーゼ」
関連記事	「kinase」「phosphoenolpyruvate」