関: heterogeneous nuclear RNA、hnRNA

WordNet

the 18th letter of the Roman alphabet (同)r

PrepTutorEJDIC

resistance / 17歳以下父兄同伴映画の表示 / rook
ribonucleic acid・リボ核酸

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1. HIV-1 RNA定量の技術および解釈 techniques and interpretation of hiv 1 rna quantitation
2. 分子遺伝学の原理 principles of molecular genetics
3. 慢性C型肝炎ウイルス感染の診断および評価 diagnosis and evaluation of chronic hepatitis c virus infection
4. 遺伝学およびゲノミクスのためのツール：遺伝子発現プロファイリング tools for genetics and genomics gene expression profiling
5. HIV感染患者における慢性C型肝炎治療効果の予測因子 predictors of response to treatment of chronic hepatitis c infection in hiv infected patients

English Journal

Transcriptional repression of the Ahr gene by LHCGR signaling in preovulatory granulosa cells is controlled by chromatin accessibility.

Teino I, Matvere A, Kuuse S, Ingerpuu S, Maimets T, Kristjuhan A, Tiido T.Author information Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia.AbstractRecent advances in establishing the role of the aryl hydrocarbon receptor (Ahr) in normophysiology have discovered its fundamental role, amongst others, in female reproduction. Considering previous studies suggesting the hormonal modulation of Ahr, we aimed to investigate whether in murine granulosa cells (GCs) the gonadotropins regulate Ahr expression and how this is mechanistically implemented. We found that the FSH-like substance--pregnant mare serum gonadotropin--led to stimulation of Ahr expression. More importantly hCG produced relatively rapid reduction of Ahr mRNA in GCs of preovulatory follicles. We show for the first time that LHCGR signaling in regulating the Ahr message involves protein kinase A pathway and is attributable to decreased transcription rate. Finally, we found that Ahr promoter accessibility was decreased by hCG, implicating chromatin remodeling in Ahr gene regulation by LH.
Molecular and cellular endocrinology.Mol Cell Endocrinol.2014 Jan 25;382(1):292-301. doi: 10.1016/j.mce.2013.10.011. Epub 2013 Oct 18.
Recent advances in establishing the role of the aryl hydrocarbon receptor (Ahr) in normophysiology have discovered its fundamental role, amongst others, in female reproduction. Considering previous studies suggesting the hormonal modulation of Ahr, we aimed to investigate whether in murine granulosa
PMID 24145128

Rapid induction of the growth hormone gene transcription by glucocorticoids in vitro:possible involvement of membrane glucocorticoid receptors and phosphatidylinositol 3-kinase activation.

Nogami H, Yamamoto N, Hiraoka Y, Aiso S, Sugimoto K, Yoshida S, Shutoh F, Hisano S.Author information Laboratry of Neuroendocrinology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, 305-8575, Japan.AbstractThe regulation of transcription of the growth hormone (GH) gene by glucocorticoids was studied in MtT/S cells, a cell line derived from estrogen induced mammotropic tumor in the rat, and in the primary culture of the anterior pituitary gland of adult mice. The levels of the GH heteronuclear RNA (GH hnRNA), which are mainly determined by the transcription rate, increased by 25-fold during 24 h in response to dexamethasone (DEX, 1μM) in MtT/S cells which have been cultured in the medium containing charcoal-stripped serum for 7 days. The stimulatory effect of DEX on the GH hnRNA levels was detectable as early as 30 min. This rapid effect of DEX did not require on-going protein synthesis, while it was considered that DEX requires the presence of unknown cellular proteins produced independent of DEX stimulation. In contrast, on-going protein synthesis was required for DEX action when incubated for 6h as has been observed in the previous studies. The specific inhibitor of glucocorticoid receptor, RU486, inhibited both rapid (30 min) and delayed (6 h) effects of glucocorticoids on GH hnRNA levels. Membrane impermeable glucocorticoid, corticosterone-bovine serum albumin conjugate (CSBSA), was found to have the similar effects as those of DEX and free corticosterone (CS), suggesting that glucocorticoids regulate GH gene transcription at least in part through the membrane bound receptors. From pharmacological studies, it was suggested that phosphatidylinositol 3-kinase (PI3-kinase) activation is involved in the rapid effects, but not in the delayed effects of glucocorticoids. This also suggests that the delayed effects of glucocorticoids depend on the mechanisms other than the activation of PI3-kinase. Finally, both rapid and delayed effects of CS and CSBSA were observed not only in MtT/S cells, but also in the mouse pituitary cells in primary culture. Therefore, it is possible that the membrane initiated action of glucocorticoids is involved in the regulation of the GH-transcription in the normal pituitary cells as well as in the pituitary tumor cells. This article is protected by copyright. All rights reserved.
Journal of neuroendocrinology.J Neuroendocrinol.2014 Jan 15. doi: 10.1111/jne.12132. [Epub ahead of print]
The regulation of transcription of the growth hormone (GH) gene by glucocorticoids was studied in MtT/S cells, a cell line derived from estrogen induced mammotropic tumor in the rat, and in the primary culture of the anterior pituitary gland of adult mice. The levels of the GH heteronuclear RNA (GH
PMID 24428719

Role of Sam68 in post-transcriptional gene regulation.

Sánchez-Jiménez F, Sánchez-Margalet V.Author information Department of Medical Biochemistry and Molecular Biology and Immunology, UGC Clinical Biochemistry, Virgen Macarena University Hospital, Avenue. Sánchez Pizjuan 4, Medical School, University of Seville, Seville 41009, Spain. margalet@us.es.AbstractThe STAR family of proteins links signaling pathways to various aspects of post-transcriptional regulation and processing of RNAs. Sam68 belongs to this class of heteronuclear ribonucleoprotein particle K (hnRNP K) homology (KH) single domain-containing family of RNA-binding proteins that also contains some domains predicted to bind critical components in signal transduction pathways. In response to phosphorylation and other post-transcriptional modifications, Sam68 has been shown to have the ability to link signal transduction pathways to downstream effects regulating RNA metabolism, including transcription, alternative splicing or RNA transport. In addition to its function as a docking protein in some signaling pathways, this prototypic STAR protein has been identified to have a nuclear localization and to take part in the formation of both nuclear and cytosolic multi-molecular complexes such as Sam68 nuclear bodies and stress granules. Coupling with other proteins and RNA targets, Sam68 may play a role in the regulation of differential expression and mRNA processing and translation according to internal and external signals, thus mediating important physiological functions, such as cell death, proliferation or cell differentiation.
International journal of molecular sciences.Int J Mol Sci.2013 Nov 28;14(12):23402-19. doi: 10.3390/ijms141223402.
The STAR family of proteins links signaling pathways to various aspects of post-transcriptional regulation and processing of RNAs. Sam68 belongs to this class of heteronuclear ribonucleoprotein particle K (hnRNP K) homology (KH) single domain-containing family of RNA-binding proteins that also conta
PMID 24287914

Japanese Journal

STRUCTURAL ANALYSIS OF A RNA-BINDING PROTEIN, RbpA1,IN Anabaena variabilis M3 WITH HETERONUCLEAR MULTI-DIMENSIONAL NMR SPECTROSCOPY

Plant and cell physiology 41(Supplement), s43, 2000
NAID 110003722094

Rapid and sensitive vasopressin heteronuclear RNA responses to changes in plasma osmolality

J Neuroendocrinol 11, 337-341, 1999
NAID 30014446327

Different Expression of Immediate-Early Genes in the Rat Paraventricular Nucleus Induced by Stress:Relation to Corticotropin-Releasing Factor Gene Transcription