直接免疫蛍光法

WordNet

straightforward in means or manner or behavior or language or action; "a direct question"; "a direct response"; "a direct approach"
having no intervening persons, agents, conditions; "in direct sunlight"; "in direct contact with the voters"; "direct exposure to the disease"; "a direct link"; "the direct cause of the accident"; "direct vote" (同)unmediated
in precisely the same words used by a writer or speaker; "a direct quotation"; "repeated their dialog verbatim" (同)verbatim
(of a current) flowing in one direction only; "direct current"
be in charge of
being an immediate result or consequence; "a direct result of the accident"
command with authority; "He directed the children to do their homework"
direct in spatial dimensions; proceeding without deviation or interruption; straight and short; "a direct route"; "a direct flight"; "a direct hit"
give directions to; point somebody into a certain direction; "I directed them towards the town hall"
guide the actors in (plays and films)
lacking compromising or mitigating elements; exact; "the direct opposite"
moving from west to east on the celestial sphere; or--for planets--around the sun in the same direction as the Earth
similar in nature or effect or relation to another quantity; "a term is in direct proportion to another term if it increases (or decreases) as the other increases (or decreases)"
(immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissue
manageable by a supervising agent; "a directed program of study"
(often used in combination) having a specified direction; "a positively directed vector"; "goal-directed"
showing the way by conducting or leading; imposing direction on; "felt his mothers directing arm around him"; "the directional role of science on industrial progress" (同)directional, directive, guiding

PrepTutorEJDIC

『まっすぐな』,一直線の(straight),直進する / (列車などが途中駅に寄らずに)直行 / 直系の / 『直接の』,じかの / 『率直な』,単刀直入の / 全くの,絶対の / 原典どおりの / 『まっすぐに』,直行で / 〈事〉‘を'『指揮する』,監督する / 《文》〈人〉‘に'『命令する』,指図する / 〈人〉‘に'(…への)『道を教える』《+『名』〈人〉+『to』+『名』》 / (…に)〈目・注意・努力など〉‘を'『向ける』《+『名』+『to』(『at, toward』)+『名』(do『ing』)》 / 〈音楽・演劇・映画など〉‘を'『指揮する』,演出する,監督する / (…に)〈手紙など〉‘を'あてる《+『名』+『to』+『名』》
恐ろしい,ものすごい;不吉な / 差し迫った(urgent);極端な

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/03/31 23:34:12」(JST)

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Direct FA stained mouse brain impression smear reveals the presence of the bacterium Chlamydia psittaci. 400X.

A direct fluorescent antibody (DFA or dFA) (also known as "Direct immunofluorescence"^[1]) is an antibody that has been tagged in a direct fluorescent antibody test. Its name derives from the fact that it directly tests the presence of an antigen with the tagged antibody, unlike western blotting, which uses an indirect method of detection, where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached to the secondary antibody.

Commercial DFA testing kits are available, which contain fluorescently labelled antibodies, designed to specifically target unique antigens present in the bacteria or virus, but not present in mammals (Eukaryotes). This technique can be used to quickly determine if a subject has a specific viral or bacterial infection.

In the case of respiratory viruses, many of which have similar broad symptoms, detection can be carried out using nasal wash samples from the subject with the suspected infection. Although shedding cells in the respiratory tract can be obtained, it is often in low numbers, and so an alternative method can be adopted where compatible cell culture can be exposed to infected nasal wash samples, so if the virus is present it can be grown up to a larger quantity, which can then give a clearer positive or negative reading.

As with all types of fluorescence microscopy, the correct absorption wavelength needs to be determined in order to excite the fluorophore tag attached to the antibody, and detect the fluorescence given off, which indicates which cells are positive for the presence of the virus or bacteria being detected.

Direct fluorescent antibody can also be used to detect parasitic infections, as was pioneered by Sadun, et al. (1960).

References

^ Pober, Jordan S.; Abbas, Abul K.; Lichtman, Andrew H. (2000). Cellular and molecular immunology. Philadelphia: Saunders. ISBN 0-7216-8233-2.

External links

Direct Fluorescent Antibody Technique at the US National Library of Medicine Medical Subject Headings (MeSH)

Wikimedia Commons has media related to: Direct fluorescent antibody stain

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