電気泳動移動度シフト解析 electrophoretic mobility shift assay
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/09/30 18:42:25」(JST)
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English Journal
- Investigation of the mechanism involved in the As2O3-regulated decrease in MDR1 expression in leukemia cells.
- Gao F1, Liu J2, Dong WW2, Wang W2, Wang Y2, Cai D3, Zheng Z2, Sun K1.Author information 1Department of Genetics, China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China.2Laboratory Animal Center, China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China.3The First Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China.AbstractArsenic trioxide (As2O3) inhibits the expression of P-glycoprotein (P-gp) in leukemia cells; however, the mechanism behind this inhibition is unclear. The present study aimed to explore the effect of As2O3 on the expression and regulation of P-gp in leukemia cells, and elucidate the mechanism of the reversal of drug resistance. In the present study, electrophoretic mobility shift assay results indicated that p65 binds to the NF-κB binding site of MDR1, specifically in K562/D cells. Expression of p65 and phosphorylated IκB was reduced, while the expression of IκB was increased in K562/D cells treated with As2O3. The activity of luciferase increased up to 9-fold with 40 ng/ml TNF-α, and it was suppressed by ~25% following treatment with 1 µM As2O3. These findings suggest that As2O3 reverses the P-gp-induced drug resistance of leukemia cells through the NF-κB pathway. As2O3 may inhibit the activity of phosphorylase to inhibit IκB phosphorylation, thereby inhibiting NF-κB activity and MDR1 gene expression, leading to reversal of drug resistance.
- Oncology reports.Oncol Rep.2014 Feb;31(2):926-32. doi: 10.3892/or.2013.2930. Epub 2013 Dec 16.
- Arsenic trioxide (As2O3) inhibits the expression of P-glycoprotein (P-gp) in leukemia cells; however, the mechanism behind this inhibition is unclear. The present study aimed to explore the effect of As2O3 on the expression and regulation of P-gp in leukemia cells, and elucidate the mechanism of the
- PMID 24337533
- Coordinated Transcriptional Regulation of Hspa1a Gene by Multiple Transcription Factors: Crucial Roles for HSF-1, NF-Y, NF-κB, and CREB.
- Sasi BK1, Sonawane PJ1, Gupta V1, Sahu BS1, Mahapatra NR2.Author information 1Cardiovascular Genetics Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India.2Cardiovascular Genetics Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India. Electronic address: nmahapatra@iitm.ac.in.AbstractAlthough the transcript level of inducible heat shock protein 70.3 (Hsp70.3, also known as Hspa1a) is altered in various disease states, its transcriptional regulation remains incompletely understood. Here, we systematically analyzed the Hspa1a promoter to identify major cis elements and transcription factors that may govern the constitutive/inducible gene expression. Computational analyses coupled with extensive in vitro (promoter-reporter activity and electrophoretic mobility shift assays) and in vivo (chromatin immunoprecipitation assays) revealed interaction of several transcription factors with Hspa1a promoter motifs: HSF-1 (heat shock factor 1) at -114/-97bp and -788/-777bp, NF-Y (nuclear transcription factor Y) at -73/-58bp, NF-κB (nuclear factor kappa B) at -133/-124bp, and CREB (cAMP response element binding protein) at -483/-476bp. Consistently, siRNA (small interfering RNA)-mediated down-regulation of each of these transcription factors caused substantial reduction of endogenous Hspa1a expression. Heat-shock-induced activation of Hspa1a was coordinately regulated by HSF-1 and NF-Y/NF-κB. The Hspa1a expression was augmented by TNF-α (tumor necrosis factor-alpha) and forskolin in NF-κB and CREB-dependent manners, respectively. NF-κB and CREB also activated Hspa1a transcription in cardiac myoblasts upon exposure to ischemia-like conditions. Taken together, this study discovered previously unknown roles for NF-κB and CREB to regulate Hspa1a expression and a coordinated action by several transcription factors for Hspa1a transactivation under heat-shock/ischemia-like conditions and thereby provided new insights into the mechanism of Hspa1a regulation.
- Journal of molecular biology.J Mol Biol.2014 Jan 9;426(1):116-35. doi: 10.1016/j.jmb.2013.09.008. Epub 2013 Sep 14.
- Although the transcript level of inducible heat shock protein 70.3 (Hsp70.3, also known as Hspa1a) is altered in various disease states, its transcriptional regulation remains incompletely understood. Here, we systematically analyzed the Hspa1a promoter to identify major cis elements and transcripti
- PMID 24041570
- Differential response of DU145 and PC3 prostate cancer cells to ionizing radiation: Role of reactive oxygen species, GSH and Nrf2 in radiosensitivity.
- Jayakumar S, Kunwar A, Sandur SK, Pandey BN, Chaubey RC.Author information Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.AbstractBACKGROUND: Radioresistance is the major impediment in radiotherapy of many cancers including prostate cancer, necessitating the need to understand the factors contributing to radioresistance in tumor cells. In the present study, the role of cellular redox and redox sensitive transcription factor, Nrf2 in the radiosensitivity of prostate cancer cell lines PC3 and DU145, has been investigated.
- Biochimica et biophysica acta.Biochim Biophys Acta.2014 Jan;1840(1):485-94. doi: 10.1016/j.bbagen.2013.10.006. Epub 2013 Oct 9.
- BACKGROUND: Radioresistance is the major impediment in radiotherapy of many cancers including prostate cancer, necessitating the need to understand the factors contributing to radioresistance in tumor cells. In the present study, the role of cellular redox and redox sensitive transcription factor, N
- PMID 24121106
Japanese Journal
- 欧州連合における海洋関連法制 : 欧州海上安全庁(EMSA)の活動を中心に (特集 海の安全と法)
- 武田 美智代
- 外国の立法 : 立法情報・翻訳・解説 (259), 28-36, 2014-03
- NAID 40020014206
- The Transcriptional Regulatory Mechanism of the Peroxisomal Ascorbate Peroxidase (pAPX) Gene Cloned from an Extreme Halophyte, Salicornia brachiata
- Tiwari Vivekanand,Chaturvedi Amit Kumar,Mishra Avinash [他]
- Plant and cell physiology 55(1), 201-217, 2014-01
- NAID 40019968956
- The establishment of the Asian maritime safety and security organization(AMSSO)
- HABARA Keiji
- Journal of maritime researches 3(1), 15-30, 2013-03
- NAID 110009621525
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| リンク元 | 「電気泳動移動度シフト解析」「electrophoretic mobility shift assay」 |
| 関連記事 | 「EMS」「EM」 |
「電気泳動移動度シフト解析」
「electrophoretic mobility shift assay」
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