DMEM

• ダルベッコ変法イーグル培地

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Dulbecco's modified Eagle medium

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/08/02 00:35:22」(JST)

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Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.

It contains:

• amino acids
• salts (calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, and monosodium phosphate)
• glucose
• vitamins (folic acid, nicotinamide, riboflavin, B₁₂)

A variation of this EMEM, called Dulbecco’s modified Eagle's medium (DMEM), (Dulbecco/Vogt modified Eagle's minimal essential medium), contains approximately four times as much of the vitamins and amino acids present in the original formula and two to four times as much glucose. Additionally, it contains iron and phenol red. DMEM is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.

See also

• RPMI (Roswell Park Memorial Institute medium), for lymph cells
• GMEM (Glasgow's Minimal Essential Medium)

References

English Journal

• Brushite cement additives inhibit attachment to cell culture beads.

• Jamshidi P, Bridson RH, Wright AJ, Grover LM.SourceSchool of Chemical Engineering, University of Birmingham, Edgbaston B15 2TT, UK; telephone: +44-121-414-3887; fax: +44-121-414-5324.
• Biotechnology and bioengineering.Biotechnol Bioeng.2013 May;110(5):1487-94. doi: 10.1002/bit.24806. Epub 2013 Jan 17.
• Brushite-forming calcium phosphate cements are of great interest as bone replacement materials because they are resorbable in physiological conditions. Cell-attached culture beads formed from this material could be of great use for cell therapy. Despite a significant amount of work on optimizing the
• PMID 23242924

• Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody.

• Zhang H, Wang H, Liu M, Zhang T, Zhang J, Wang X, Xiang W.SourceSchool of Life Science, Northeast Agricultural University, Harbin, 150030, China.
• Cytotechnology.Cytotechnology.2013 May;65(3):363-78. doi: 10.1007/s10616-012-9488-4. Epub 2012 Aug 21.
• A serum-free medium (CHO-SFM) together with a fed-batch process was developed for the cultivation of a recombinant GS-CHO cell line producing TNFR-Fc. According to the metabolic characteristics of GS-CHO cell, a basal medium was prepared by supplementing DMEM:F12:RPMI1640 (2:1:1) with amino acids, i
• PMID 22907508

• Lectin from Canavalia brasiliensis (ConBr) protects hippocampal slices against glutamate neurotoxicity in a manner dependent of PI3K/Akt pathway.

• Jacques AV, Rieger DK, Maestri M, Lopes MW, Peres TV, Gonçalves FM, Pedro DZ, Tasca CI, López MG, Egea J, Nascimento KS, Cavada BS, Leal RB.SourceDepartamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, SC, Brazil.
• Neurochemistry international.Neurochem Int.2013 May;62(6):836-42. doi: 10.1016/j.neuint.2013.02.020. Epub 2013 Feb 26.
• The excitotoxicity induced by excessive activation of the glutamatergic neurotransmission pathway is involved in several neuropathologies. In this sense, molecules that prevent the release of glutamate or the excessive activation of its receptors can be useful in preventing the neuronal cell death o
• PMID 23454192

Japanese Journal

• Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in porous hyaluronic acid scaffold(CELL AND TISSUE ENGINEERING)

• Yoon In-Soo,Chung Chung Wook,Sung Jong-Hyuk,Cho Hyun-Jong,Kim Jung Sun,Shim Won-Sik,Shim Chang-Koo,Chung Suk-Jae,Kim Dae-Duk
• Journal of bioscience and bioengineering 112(4), 402-408, 2011-10
• … serum-free DMEM-HG supplemented with 10ng/mL of transforming growth factor-β1 (TGF-β1), 50nM ascorbate, 100nM dexamethasone, and 5μg/mL of ITS), better proliferation and chondrogenic differentiation of AD-MSCs were obtained in the 3D HA scaffold culture as compared to the micromass culture, a standard 3D culture system. …
• NAID 110008761499

• Research on the Isolation of Mouse Leydig Cells Using Differential Digestion with a Low Concentration of Collagenase

• SUN Jie,ZHONG Liang,ZHU Yingjian,LIU Guohua
• The Journal of reproduction and development 57(3), 433-436, 2011-06-01
• … Cells obtained were cultured in low glucose DMEM with 10% FBS. …
• NAID 10029050947

Related Links

• DMEM - Dulbecco's Modified Eagle Medium | Life Technologies

Gibco® DMEMは、哺乳類細胞培養に優れた品質と一貫性をお届けします。 ... Gibco® GlutaMAX 培地は、標準的な細胞培養用培地であり、接着培養および浮遊培養のいずれにおいても分解およびアンモニア蓄積を防ぐために、安定型のL ...

• ダルベッコ改変イーグル培地 | オンラインカタログ｜lonza ...

DMEM（ダルベッコ改変イーグル培地）は、哺乳類細胞の培養に幅広く使用されています。高グルコース培地は、高密度の浮遊培養に適しており、低グルコース培地は接着培養に使用されます。

Related Pictures

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★リンクテーブル★

リンク元	「Dulbecco's modified Eagle medium」「ダルベッコ変法イーグル培地」「ダルベッコのMEM培地」
関連記事	「DM」

「Dulbecco's modified Eagle medium」

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ダルベッコ変法イーグル培地、Dulbecco変法イーグル培地

関

DMEM

「ダルベッコ変法イーグル培地」

　　[★]

英

Dulbecco's modified Eagle medium、DMEM

関

Dulbecco変法イーグル培地

「ダルベッコのMEM培地」

　　[★]

英

Dulbecco Modified Eagle Medium DMEM

「DM」

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