関: beta1 integrin、integrin beta1

WordNet

any substance (as a toxin or enzyme) that stimulates an immune response in the body (especially the production of antibodies)

PrepTutorEJDIC

抗原(生物の体内にはいって免疫体を作る物質)
certificate of deposit / (また『C.D.』)Civil Defense民間防衛

UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

1. 前立腺癌のスクリーニング screening for prostate cancer
2. 前立腺特異的抗原（PSA）の測定 measurement of prostate specific antigen
3. 抗原提示細胞 antigen presenting cells
4. 胎児および新生児における免疫細胞の発達 the development of immune cells in the fetus and neonate
5. BおよびTリンパ球の正常な発生 normal b and t lymphocyte development

English Journal

Isolation and characterization of lung resident mesenchymal stem cells capable of differentiating into alveolar epithelial type II cells.

Gong X, Sun Z, Cui D, Xu X, Zhu H, Wang L, Qian W, Han X.Author information Immunology and Reproductive Biology Laboratory, Medical College of Nanjing University, Nanjing, 210093, China.AbstractControversies and risks continue to be reported about exogenous mesenchymal stem cell-based therapies. In contrast with employing exogenous stem cell, making use of lung resident mesenchymal stem cells (LR-MSCs) could be advantageous. Our study seeked to isolate the LR-MSCs and explore their potential to differentiate into alveolar epithelial type II cells (ATII cells). Total lung cells were first precultured, from which the Sca-1+ CD45- CD31- population was purified using fluorescence activated cell sorting (FACS). By these methods, it would seem that the Sca-1+ CD45- CD31- cells were LR-MSCs. Similar to bone marrow derived mesenchymal stem cells (BM-MSCs), these cells express Sca-1, CD29, CD90, CD44 and CD106, but not CD31 or CD45. They share the same gene expression file with the BM-MSCs and have a similar DNA content during long-term culturing. Furthermore, they could be serially passaged with all these properties being sustained. Above all, LR-MSCs could differentiate into ATII cells when co-cultured with ATII cells in a trans-well system. These findings demonstrated that the Sca-1+ CD45- CD31- cells appear to be LR-MSCs that can differentiate into ATII cells. This approach may hold promise for their use in the treatment of lung disease.
Cell biology international.Cell Biol Int.2014 Jan 8. doi: 10.1002/cbin.10240. [Epub ahead of print]
Controversies and risks continue to be reported about exogenous mesenchymal stem cell-based therapies. In contrast with employing exogenous stem cell, making use of lung resident mesenchymal stem cells (LR-MSCs) could be advantageous. Our study seeked to isolate the LR-MSCs and explore their potenti
PMID 24403246

Oxytocin-Gly-Lys-Arg stimulates cardiomyogenesis by targeting cardiac side population cells.

Danalache BA, Yu C, Gutkowska J, Jankowski M.Author information B Danalache, Cardiovascular Biochemistry, Centre de recherche, CHUM-Hotel-Dieu, Montreal, Canada.AbstractThe functional oxytocin (OT) system is expressed in the human and rodent heart. OT stimulates differentiation of cardiac stem cells into contracting cardiomyocytes (CM). In the present study, we investigated OT receptors (OTR) expressed in cells of cardiac side population (SP) and the abilities of these cells to differentiate into CM in response to the treatment with OT-Gly-Lys-Arg (OT-GKR), a dominant and biologically active form of OT in the fetal rodent heart. Immunocytochemistry of whole rat embryo at mid-gestation (E11), revealed parallel staining in the heart of OTR and the ABCG2 (brcp1) antigen, the marker of the side population (SP) phenotype. Using flow cytometry, the SP cells were selected from the newborn CM stained with Höechst 33342. 5.32% ± 0.06% of side population and 15.2% ± 1.10 of main population expressed OTR on the cell surface. The OTR was detected in CD29 (6.6%) then in CD31 (4.7%) but less frequently in CD45 (0.7%) positive SP cell subpopulations. Specifically, the phenotype of SP CD31- cell, but not SP CD31+ cells, proliferates in the presence of OT-GKR and develops large cell aggregates. Then, OT-GKR treatment induced the apparition of beating cell colonies after 11 days (10 ± 2.78 %), which increased until day 16 (52 ± 1.21%). The cells in contractile colonies expressed the markers of a CM phenotype such as troponin, cardiac myosin light chain 2 and actinin. Finally, SP cells stimulated by OT-GKR induced endothelial phenotype. These results suggest that the C-terminally extended OT molecule stimulate cardiac differentiation of SP CD31- cells and is involved in heart growth.
The Journal of endocrinology.J Endocrinol.2014 Jan 8. [Epub ahead of print]
The functional oxytocin (OT) system is expressed in the human and rodent heart. OT stimulates differentiation of cardiac stem cells into contracting cardiomyocytes (CM). In the present study, we investigated OT receptors (OTR) expressed in cells of cardiac side population (SP) and the abilities of t
PMID 24403294

Functional differences in mesenchymal stromal cells from human dental pulp and periodontal ligament.

Vasandan AB, Shankar SR, Prasad P, Sowmya Jahnavi V, Bhonde RR, Jyothi Prasanna S.Author information School of Regenerative Medicine (SORM), Manipal University, Bangalore, India.AbstractClinically reported reparative benefits of mesenchymal stromal cells (MSCs) are majorly attributed to strong immune-modulatory abilities not exactly shared by fibroblasts. However, MSCs remain heterogeneous populations, with unique tissue-specific subsets, and lack of clear-cut assays defining therapeutic stromal subsets adds further ambiguity to the field. In this context, in-depth evaluation of cellular characteristics of MSCs from proximal oro-facial tissues: dental pulp (DPSCs) and periodontal ligament (PDLSCs) from identical donors provides an opportunity to evaluate exclusive niche-specific influences on multipotency and immune-modulation. Exhaustive cell surface profiling of DPSCs and PDLSCs indicated key differences in expression of mesenchymal (CD105) and pluripotent/multipotent stem cell-associated cell surface antigens: SSEA4, CD117, CD123 and CD29. DPSCs and PDLSCs exhibited strong chondrogenic potential, but only DPSCs exhibited adipogenic and osteogenic propensities. PDLSCs expressed immuno-stimulatory/immune-adhesive ligands like HLA-DR and CD50, upon priming with IFNγ, unlike DPSCs, indicating differential response patterns to pro-inflammatory cytokines. Both DPSCs and PDLSCs were hypo-immunogenic and did not elicit robust allogeneic responses despite exposure to IFNγ or TNFα. Interestingly, only DPSCs attenuated mitogen-induced lympho-proliferative responses and priming with either IFNγ or TNFα enhanced immuno-modulation capacity. In contrast, primed or unprimed PDLSCs lacked the ability to suppress polyclonal T cell blast responses. This study indicates that stromal cells from even topographically related tissues do not necessarily share identical MSC properties and emphasizes the need for a thorough functional testing of MSCs from diverse sources with respect to multipotency, immune parameters and response to pro-inflammatory cytokines before translational usage.
Journal of cellular and molecular medicine.J Cell Mol Med.2014 Jan 3. doi: 10.1111/jcmm.12192. [Epub ahead of print]
Clinically reported reparative benefits of mesenchymal stromal cells (MSCs) are majorly attributed to strong immune-modulatory abilities not exactly shared by fibroblasts. However, MSCs remain heterogeneous populations, with unique tissue-specific subsets, and lack of clear-cut assays defining thera
PMID 24393246